Profile of Edward M. De Robertis - Howard Hughes Medical Institute
... follow in his father’s footsteps. “Consequently, I did not have to invest any energy in choosing a career,” he says. Students interested in biology were steered toward the field of medicine, so De Robertis went down that path. He earned a degree in medicine in 1971 from the University of Uruguay’s Sc ...
... follow in his father’s footsteps. “Consequently, I did not have to invest any energy in choosing a career,” he says. Students interested in biology were steered toward the field of medicine, so De Robertis went down that path. He earned a degree in medicine in 1971 from the University of Uruguay’s Sc ...
Meiosis
... of chromosomes can produce many combinations of chromosomes when it produces sex cells, just as many different hands can be dealt from one pack of cards. When one of these sex cells unites with another, a new organism containing two sets of genetic information is formed. This new organism’s genetic ...
... of chromosomes can produce many combinations of chromosomes when it produces sex cells, just as many different hands can be dealt from one pack of cards. When one of these sex cells unites with another, a new organism containing two sets of genetic information is formed. This new organism’s genetic ...
Characterization of a cDNA Clone Encoding Multiple Copies of the
... clones were screened on Hybond-N membranes (Amersham Intemational Corp.), at a density of 5000 plaque-forming units 135 mm filter. Plaque lifts were done as recommended by the manufacturer. The membranes were hybridized in 6 x SSC (1 x SSC: 0.15 M NaCl and 0.0 15 M Na-citrate), 5 x Denhardt’s (accor ...
... clones were screened on Hybond-N membranes (Amersham Intemational Corp.), at a density of 5000 plaque-forming units 135 mm filter. Plaque lifts were done as recommended by the manufacturer. The membranes were hybridized in 6 x SSC (1 x SSC: 0.15 M NaCl and 0.0 15 M Na-citrate), 5 x Denhardt’s (accor ...
Study on the Genetic Transformation of Gentian by Gene Recombinant
... Figure 7. The hairy roots of transgene gentian mediated by Ti and the hairy root transformed by R1000 5. Study on induced hairy root mediated — ...
... Figure 7. The hairy roots of transgene gentian mediated by Ti and the hairy root transformed by R1000 5. Study on induced hairy root mediated — ...
rolduc meeting. feel connected!
... ‘feel connected’. First and foremost it symbolizes our aim to make all participants at the Retreat feel part of the ever expanding human genetics family. This family comprises ‘veterans’, but also many new PhD students from the different centres in the Netherlands and, this year for the first time, ...
... ‘feel connected’. First and foremost it symbolizes our aim to make all participants at the Retreat feel part of the ever expanding human genetics family. This family comprises ‘veterans’, but also many new PhD students from the different centres in the Netherlands and, this year for the first time, ...
Gene Section VIP (vasoactive intestinal peptide) Atlas of Genetics and Cytogenetics
... VIP binds with high affinity to 2 GPCR (VPAC1 and VPAC2) which are members of the class II or class B secretin-like receptors but not PAC1 which binds PACAP with high affinity (Harmar et al., 2012). The activated VPAC1 or VPAC2 interacts with a stimulatory guanine nucleotide binding protein (Gs) cau ...
... VIP binds with high affinity to 2 GPCR (VPAC1 and VPAC2) which are members of the class II or class B secretin-like receptors but not PAC1 which binds PACAP with high affinity (Harmar et al., 2012). The activated VPAC1 or VPAC2 interacts with a stimulatory guanine nucleotide binding protein (Gs) cau ...
reproductive cell fate transition in plants - Development
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
Molecular Genetic Testing For BRAF Mutations
... TaqMan® Mutation Detection Assay to detect the V600E mutation in exon 15 of BRAF in tumor (somatic) cells. The sensitivity of the TaqMan assay is ~0.1% mutant DNA in a wild-type background. Poor DNA quality, insufficient DNA quantity or the presence of PCR inhibitors can result in uninterpretable or ...
... TaqMan® Mutation Detection Assay to detect the V600E mutation in exon 15 of BRAF in tumor (somatic) cells. The sensitivity of the TaqMan assay is ~0.1% mutant DNA in a wild-type background. Poor DNA quality, insufficient DNA quantity or the presence of PCR inhibitors can result in uninterpretable or ...
Gene Section NOTCH2 (Notch homolog 2 (Drosophila)) Atlas of Genetics and Cytogenetics
... furin-like convertase in the trans-golgi network before it reaches the plasma membrane to yield an active form. Cleavage results in a C-terminal fragment and a Nterminal fragment, linked by disulfide bridges. Following ligand binding, it is first cleaved by ADAM17 metallopeptidase to yield a membran ...
... furin-like convertase in the trans-golgi network before it reaches the plasma membrane to yield an active form. Cleavage results in a C-terminal fragment and a Nterminal fragment, linked by disulfide bridges. Following ligand binding, it is first cleaved by ADAM17 metallopeptidase to yield a membran ...
2.3 Carbon-Based Molecules
... 2.3 Carbon-Based Molecules Carbon atoms have unique bonding properties. • Carbon forms covalent bonds with up to four other atoms, including other carbon atoms. • Carbon-based molecules have three general types of structures. – straight chain – branched chain – ring ...
... 2.3 Carbon-Based Molecules Carbon atoms have unique bonding properties. • Carbon forms covalent bonds with up to four other atoms, including other carbon atoms. • Carbon-based molecules have three general types of structures. – straight chain – branched chain – ring ...
Structure, expression and chromosomal location of the Oct
... kb. It is unclear what the larger fragment represents because there is no BssHI1 site in the gene. However, there is a BssHII site in a cosmid containing Oct-4 (see below). The same pulsed field gel was then probed with pH2IIa, a generic probe for class I genes (Steinmetz et al., 1981). Although pre ...
... kb. It is unclear what the larger fragment represents because there is no BssHI1 site in the gene. However, there is a BssHII site in a cosmid containing Oct-4 (see below). The same pulsed field gel was then probed with pH2IIa, a generic probe for class I genes (Steinmetz et al., 1981). Although pre ...
CTY Genetics Syllabus
... (M. Chalfie et al) (30 minutes) GFP as a living cell label • Lecture/Class Notes- Variation, Selection, Speciation, Darwin and Hardy/Weinberg (45 minutes) • Student Worksheet on Hardy-Weinberg (15 minutes) • Student DNA Sample Isolation for Hardy-Weinberg Lab (50 minutes) Afternoon (120 minutes) • H ...
... (M. Chalfie et al) (30 minutes) GFP as a living cell label • Lecture/Class Notes- Variation, Selection, Speciation, Darwin and Hardy/Weinberg (45 minutes) • Student Worksheet on Hardy-Weinberg (15 minutes) • Student DNA Sample Isolation for Hardy-Weinberg Lab (50 minutes) Afternoon (120 minutes) • H ...
Gene Section CDC25A (cell division cycle 25A) Atlas of Genetics and Cytogenetics
... patients CDC25A is reported to be overexpressed. - Although the mechanism of CDC25A upregulation in human patients is not yet clear, however, in some breast cancer cell lines it was reported that CDC25A overexpression is mainly due to increased protein stability as oppose to gene amplification or tr ...
... patients CDC25A is reported to be overexpressed. - Although the mechanism of CDC25A upregulation in human patients is not yet clear, however, in some breast cancer cell lines it was reported that CDC25A overexpression is mainly due to increased protein stability as oppose to gene amplification or tr ...
Gene Duplication - Semantic Scholar
... Gene duplication typically occurs by one of three mutational mechanisms: unequal crossing-over, retroposition, and chromosomal (or genome) duplication. Zhang 2003 summarizes the main features of these mechanisms. Kaessmann, et al. 2009 provides detailed information a ...
... Gene duplication typically occurs by one of three mutational mechanisms: unequal crossing-over, retroposition, and chromosomal (or genome) duplication. Zhang 2003 summarizes the main features of these mechanisms. Kaessmann, et al. 2009 provides detailed information a ...
Sequences of Primate Insulin Genes Support
... and at residue 37 in the C-peptide portion of the precursor, which are VaI, Pro, and Leu, respectively. In addition, there are several other notable differences between the human, chimpanzee, and African green monkey insulin gene sequences. The first is a deletion of 48 bp in the chimpanzee insulin ...
... and at residue 37 in the C-peptide portion of the precursor, which are VaI, Pro, and Leu, respectively. In addition, there are several other notable differences between the human, chimpanzee, and African green monkey insulin gene sequences. The first is a deletion of 48 bp in the chimpanzee insulin ...
Understanding SCID, or Bubble Boy Disease
... typically acquire serious and even lifethreatening infections, such as pneumonia, meningitis and bloodstream infections, in the first few months of life. There are five types of SCID, each caused by a different genetic defect. X-linked SCID is the most common type, caused by a genetic flaw that dama ...
... typically acquire serious and even lifethreatening infections, such as pneumonia, meningitis and bloodstream infections, in the first few months of life. There are five types of SCID, each caused by a different genetic defect. X-linked SCID is the most common type, caused by a genetic flaw that dama ...
Explaining the Likelihood Ratio in DNA Mixture
... social science. The LR is a standard measure of information that summarizes in a single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA ...
... social science. The LR is a standard measure of information that summarizes in a single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA ...
An assessment of factors affecting the likelihood
... naturally released in situ e.g. in soil or in planta, has not been shown. Information on the general composition, dynamics and succession of bacterial communities in the phytosphere of various agriculturally grown plants needs to be further determined and understood before the potential bacterial re ...
... naturally released in situ e.g. in soil or in planta, has not been shown. Information on the general composition, dynamics and succession of bacterial communities in the phytosphere of various agriculturally grown plants needs to be further determined and understood before the potential bacterial re ...
Explaining the Likelihood Ratio in DNA Mixture Interpretation
... social science. The LR is a standard measure of information that summarizes in a single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA ...
... social science. The LR is a standard measure of information that summarizes in a single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA ...
Application No. DIR 108 SUMMARY INFORMATION
... gene encodes glyphosate oxidoreductase, an enzyme capable of degrading glyphosate into nontoxic metabolites. The cp4 epsps gene encodes a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme. EPSPS enzymes participate in a biosynthetic pathway found in both plants and microorganisms that is re ...
... gene encodes glyphosate oxidoreductase, an enzyme capable of degrading glyphosate into nontoxic metabolites. The cp4 epsps gene encodes a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme. EPSPS enzymes participate in a biosynthetic pathway found in both plants and microorganisms that is re ...
Catalog# BTNM-8C PROTOCOL - G
... In the 1970s, scientists wanted to develop an economical method of producing large quantities of amylase. E. coli is a bacterium with an excellent transformation "track record." While it does not make amylase in nature, E. coli can be transformed in the laboratory to produce amylase. Transforming E. ...
... In the 1970s, scientists wanted to develop an economical method of producing large quantities of amylase. E. coli is a bacterium with an excellent transformation "track record." While it does not make amylase in nature, E. coli can be transformed in the laboratory to produce amylase. Transforming E. ...
Protein Electrophoresis, Teacher`s Guidebook (Cat. # BE-406)
... In the 1970s, scientists wanted to develop an economical method of producing large quantities of amylase. E. coli is a bacterium with an excellent transformation "track record." While it does not make amylase in nature, E. coli can be transformed in the laboratory to produce amylase. Transforming E. ...
... In the 1970s, scientists wanted to develop an economical method of producing large quantities of amylase. E. coli is a bacterium with an excellent transformation "track record." While it does not make amylase in nature, E. coli can be transformed in the laboratory to produce amylase. Transforming E. ...
htp Pseudomonas aeruginosa AMANDA SWANEPOEL
... colonizing these diverse environments is attributed to its ability to synthesize a large number of different virulence factors such as alginate, pili and lipopolysaccharides, and secreted virulence factors, including toxins, proteases and haemolysins (Lazdunski et al., 1990; Van Delden and Iglewski, ...
... colonizing these diverse environments is attributed to its ability to synthesize a large number of different virulence factors such as alginate, pili and lipopolysaccharides, and secreted virulence factors, including toxins, proteases and haemolysins (Lazdunski et al., 1990; Van Delden and Iglewski, ...
Argonaute2 Is Essential for Mammalian
... embryonic germ layers: ectoderm, endoderm, and mesoderm. The gene Brachyury is essential for mesoderm development, and shorttail mice, which were later found to be carrying a Brachyury mutation, have been known since 1927. In this study, we found a genetic interaction between Brachyury and another g ...
... embryonic germ layers: ectoderm, endoderm, and mesoderm. The gene Brachyury is essential for mesoderm development, and shorttail mice, which were later found to be carrying a Brachyury mutation, have been known since 1927. In this study, we found a genetic interaction between Brachyury and another g ...