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12 BOC314 Practical 1
12 BOC314 Practical 1

... To find the genes within the genomic sequence is a massive task in itself. Once apparent, otherwise uncharacterised coding regions must be assigned a function. Thereafter, the interactions between genes and gene products must be understood at all levels, not merely in the context of the pathways wit ...
2016 Course Outline
2016 Course Outline

...  Explain the role of enzymes as catalysts that lower the activation energy of biochemical reactions. Identify factors, such as pH and temperature, that have an effect on enzymes ...
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... First Doctoral Thesis in Molecular Biology, University of Paris Doctoral Thesis in Molecular Biology, University of Paris ...
A. thaliana genotyping with a CAPS marker for a pks3
A. thaliana genotyping with a CAPS marker for a pks3

... wild-type control. By analyzing the F2 generation for the presence of the pks3-7 mutation using a specific CAPS marker, it was possible to follow the mutant allele through various crosses. The results of a CAPS analysis using the QIAxcel system are shown in Figure 1. In lanes 2, 4, and 6, DNA fragme ...
Diffusion Demonstration
Diffusion Demonstration

... Osmosis: when water moves across the plasma membrane by passive transport ...
Protein Synthesis (Transcription and Translation)
Protein Synthesis (Transcription and Translation)

... nucleotides in each gene contains information for assembling the string of amino acids that make up a single protein. • The ribosomes required to make proteins cannot read DNA. • Therefore, for DNA to code for proteins, an RNA molecule must be made. • Ribosomes can read RNA. ...
protein synthesis
protein synthesis

... Stages of Protein Synthesis I. Transcription (nucleus) ...
RFLPs, PCR, Gel Electrophoresis
RFLPs, PCR, Gel Electrophoresis

... PCR is a technique used to clone (amplify) DNA ...
The basic unit of an immunoglobulin (Ig) molecule is composed of
The basic unit of an immunoglobulin (Ig) molecule is composed of

... of subgroup VI for which the complete sequence is known (22). Since V chains are assigned to the same subgroup if they share 70$ or greater homology (22,23), we conclude that EB4 c e l l s produce subgroup VI V^ chains. A characteristic feature of the fully sequenced members of subgroup VI, which is ...
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... 15. Explain what DNA polymerase is by breaking the word into its parts. _______________________________________________________________ 16. Write a short analogy to explain what replication is. _______________________________________________________________ ...
Restriction Enzymes, Gel Electrophoresis and Mapping DNA
Restriction Enzymes, Gel Electrophoresis and Mapping DNA

... We can make DNA, we can try to characterize it using biochemistry, we can study crude sequence information with C0t1/2 and hybridization studies; but we are limited in our pursuit of specific, single genes. If what we really want is to study, for example, the gene that is defective in cystic fibrosi ...
CH 11 Review
CH 11 Review

... 32. During metaphase I of meiosis, paired homologous chromosomes line up across the center of the cell. 33. As the cell moves into anaphase I, the homologous pairs of chromosomes separate and spindle fibers pull each homologous chromosome pair toward opposite ends of the cell. 34. The next phase is ...
Biology High School Standards Review Worksheet 1. The Chemistry
Biology High School Standards Review Worksheet 1. The Chemistry

... 6. Metaphase II ...
The Unseen Genome: Beyond DNA
The Unseen Genome: Beyond DNA

... outside the DNA sequence altogether. The tools of genetic engineering worked best on conventional genes and proteins, however, so scientists looked hardest where the light was brightest. In recent years, geneticists have been exploring the less visible parts of the genome more thoroughly, in search ...
Computational methods for the analysis of bacterial gene regulation
Computational methods for the analysis of bacterial gene regulation

... Transcriptome experiments provide an indication of the expression of all the annotated genes in an organism. Most of these experiments are performed using DNA microarrays, but other techniques such as DNA macroarrays and large scale quantitative rtPCR are also available 21. A ...
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BIO EXAM NOTES

... zygote: a cell formed by the fusion of two gametes fertilization: in humans, the joining of male and female gametes haploid: a cell that contains half the number of chromosomes as the parent cell diploid: a cell that contains pairs of homologous chromosomes synapsis: the aligning of homologous chrom ...
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ss_tn_biol_04_using_variation

... How genetic modification is an extension of selective breeding. Life-saving benefits of genetic engineering/genetic modification, such as producing insulin to treat diabetes. How genetic modification is used to produce new strains of crops, and the ongoing debate about genetic modification. ...
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Section 3.3: Carbon Compounds Building Blocks of Cells • The parts

... In a complex organism, cells recognize neighboring cells by the short, branched chains of varying sugar units on their outer surface. ...
BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 9 – Mutations
BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 9 – Mutations

... UV light causes pyrimidine dimers, such as thymine dimers Ionizing radiation (x rays, gamma rays) cause ds DNA breaks Bleomycin (anti cancer drug) causes ds breaks Base analogs – what are they? A common example is 5-bromouracil (can base pair sometimes with G) Intercalating agents – know examples; i ...
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DNA_fingerprinting

Human Genetic Mutations
Human Genetic Mutations

... What does it cause? _____________________________________________________ Nondisjunction-Chromosomes _____________ TO SEPARATE during ________________ ...
Think about what you have learned about the structure of DNA
Think about what you have learned about the structure of DNA

... characteristics of cells and their functions. (Cells carry on functions needed to sustain life. They grow, divide, take in nutrients, provide energy, and remove wastes. Specialized cells perform specialized functions in multicellular organisms.) Students understand that hereditary information is con ...
Human Genome
Human Genome

... • In females – one X chromosome is randomly turned off. • It forms a dense region in the nucleus called a Barr body. • Males don’t have Barr bodies because their X chromosome is active. • Ex. Calico Cats- X chromosome carries the allele for coat color and can carry more than 1 color. The X chromosom ...
Process 1 - Scioly.org
Process 1 - Scioly.org

... processes and relate these events to cell and organism continuity. * Comparing & analyzing data - stages of mitosis & meiosis their similarities and differences * Analyzing cell cycle data–timing for normal vs. abnormal cells * Understanding and interpreting information on prokaryotic cell reproduct ...
A4.3.1HowDoChromosomesCarryInformation
A4.3.1HowDoChromosomesCarryInformation

... 6. Where are centromeres located on chromosomes? Make a sketch of a chromosome and indicate where its centromere is located. 7. Where are telomeres located on chromosomes? Make a sketch of a chromosome and indicate where its telomeres are located. 8. From the variation window, select one of the chro ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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