FUNCTION IN PHYSARUM POLYCEPHALUM mitochondria of
... Physarum mitochondria. The biochemical properties of this protein are not clear. It is not known how mitochondrial DNA is held in its supercoiled configuration. However, the undigested filaments released by osmotic shock often were irregular and the diameter of the fibers varied from 200 to 70 A, wh ...
... Physarum mitochondria. The biochemical properties of this protein are not clear. It is not known how mitochondrial DNA is held in its supercoiled configuration. However, the undigested filaments released by osmotic shock often were irregular and the diameter of the fibers varied from 200 to 70 A, wh ...
For Official Use ENV/JM/BIO(2006)6/REV3 Working
... belonging to the same species, and transformation frequencies can vary up to four orders of magnitude among transformable isolates of a species (Sikorski et al., 2002; Maamar and Dubnau, 2005). The uptake of DNA can serve as a nutrient source, for DNA repair or as a source of genetic innovation (Dub ...
... belonging to the same species, and transformation frequencies can vary up to four orders of magnitude among transformable isolates of a species (Sikorski et al., 2002; Maamar and Dubnau, 2005). The uptake of DNA can serve as a nutrient source, for DNA repair or as a source of genetic innovation (Dub ...
Mycobacterium kyorinense sp. nov., a novel, slow
... Sequence analysis revealed that the 16S rRNA gene sequence of the newly identified mycobacterium was closest to that of M. celatum ATCC 51130 (type 2) with 18 base mismatches out of 1469 bp (98.8 % identity). It was also highly similar to that of M. celatum NCTC 12882 (type 3) with 25 base mismatche ...
... Sequence analysis revealed that the 16S rRNA gene sequence of the newly identified mycobacterium was closest to that of M. celatum ATCC 51130 (type 2) with 18 base mismatches out of 1469 bp (98.8 % identity). It was also highly similar to that of M. celatum NCTC 12882 (type 3) with 25 base mismatche ...
Efficient Screening of CRISPR/Cas9
... to simultaneously target a gene of interest and a marker gene, ebony, which is a recessive gene that produces dark body color and has the further advantage of not being a commonly used transgenic marker. We found that Drosophila broods containing higher numbers of CRISPR-induced ebony mutations (“ja ...
... to simultaneously target a gene of interest and a marker gene, ebony, which is a recessive gene that produces dark body color and has the further advantage of not being a commonly used transgenic marker. We found that Drosophila broods containing higher numbers of CRISPR-induced ebony mutations (“ja ...
Bruce Wallace Biotechnology Lab Program Student Guide 5th
... reason is related to the cost of these materials and the difficulty involved with obtaining them. For example, you will be given some specially engineered plasmids (DNA) in the next laboratory. If this DNA were sold “by the pound,” it would cost around $360,000,000 per pound. So don’t be surprised i ...
... reason is related to the cost of these materials and the difficulty involved with obtaining them. For example, you will be given some specially engineered plasmids (DNA) in the next laboratory. If this DNA were sold “by the pound,” it would cost around $360,000,000 per pound. So don’t be surprised i ...
DNA Chips: Genes to Disease
... are expressed (transcribed) in different tissues under different conditions. This is what gives different tissues their different phenotypes (appearance and function). *Note: Gametes contain half of the genes that somatic cells do, and enucleated cells (such as mature red blood cells) do not contain ...
... are expressed (transcribed) in different tissues under different conditions. This is what gives different tissues their different phenotypes (appearance and function). *Note: Gametes contain half of the genes that somatic cells do, and enucleated cells (such as mature red blood cells) do not contain ...
c-Myc co-ordinates mRNA cap methylation and ribosomal RNA
... Figure 1. Expression of the mRNA cap methyltransferase complex, RNMT–RAM, is required for 45S rRNA production. HeLa cells were transfected into two independent RAM siRNAs, an RNMT siRNA and a non-targeting control siRNA, for 48 h. (A) Expression of RNMT, RAM and GAPDH was analysed by western blot. ( ...
... Figure 1. Expression of the mRNA cap methyltransferase complex, RNMT–RAM, is required for 45S rRNA production. HeLa cells were transfected into two independent RAM siRNAs, an RNMT siRNA and a non-targeting control siRNA, for 48 h. (A) Expression of RNMT, RAM and GAPDH was analysed by western blot. ( ...
Geneticseasy
... and move toward positive end of gel d. The fragments separate because smaller molecules move faster than larger ones. This results in a pattern of bands e. Photographic film is used to visualize the DNA fragments. This pattern can serve as a person’s DNA fingerprint ...
... and move toward positive end of gel d. The fragments separate because smaller molecules move faster than larger ones. This results in a pattern of bands e. Photographic film is used to visualize the DNA fragments. This pattern can serve as a person’s DNA fingerprint ...
Free Sample
... ____ 13. In the MN blood group system, a person who inherits an "M" allele and an "N" allele expresses both M and N antigens on the RBCs. Which of the following is true? a. M is dominant to N. b. N is dominant to M. c. M an N are codominant alleles. d. M and N are located on the same chromosome. ___ ...
... ____ 13. In the MN blood group system, a person who inherits an "M" allele and an "N" allele expresses both M and N antigens on the RBCs. Which of the following is true? a. M is dominant to N. b. N is dominant to M. c. M an N are codominant alleles. d. M and N are located on the same chromosome. ___ ...
Cell differentiation during sexual development of the
... During sexual development, mycelial cells from most filamentous fungi differentiate into typical fruiting bodies. Here, we describe the isolation and characterization of the Sordaria macrospora developmental mutant per5, which exhibits a sterile phenotype with defects in fruiting body maturation. Cy ...
... During sexual development, mycelial cells from most filamentous fungi differentiate into typical fruiting bodies. Here, we describe the isolation and characterization of the Sordaria macrospora developmental mutant per5, which exhibits a sterile phenotype with defects in fruiting body maturation. Cy ...
controversy and its implications Genetic hitchhiking versus
... et al. 2003) and several plant species such as wild tomatoes (Stephan & Langley 1998; Roselius et al. 2005)), it has provoked extensive modelling and analysis efforts. The development of methods for distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An importa ...
... et al. 2003) and several plant species such as wild tomatoes (Stephan & Langley 1998; Roselius et al. 2005)), it has provoked extensive modelling and analysis efforts. The development of methods for distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An importa ...
controversy and its implications Genetic hitchhiking versus
... et al. 2003) and several plant species such as wild tomatoes (Stephan & Langley 1998; Roselius et al. 2005)), it has provoked extensive modelling and analysis efforts. The development of methods for distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An importa ...
... et al. 2003) and several plant species such as wild tomatoes (Stephan & Langley 1998; Roselius et al. 2005)), it has provoked extensive modelling and analysis efforts. The development of methods for distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An importa ...
Comparative Genomics
... Comparative Genomics Allows us to achieve a greater understanding of vertebrate evolution Tells us what is common and what is unique between different species at the genome level The function of human genes and other regions may be revealed by studying their counterparts in lower organisms ...
... Comparative Genomics Allows us to achieve a greater understanding of vertebrate evolution Tells us what is common and what is unique between different species at the genome level The function of human genes and other regions may be revealed by studying their counterparts in lower organisms ...
Molecular Plant-Microbe Interactions 13:
... 1.6.1.19) activity was determined with a colorimetric reaction according to Yonaha and Toyama (1980). Glutamate decarboxylase (GAD, EC 4.1.1.15) activity was measured by the method of Fonda (1985) as modified by Vézina et al. (1988). The bacteria used to measure GAD activity were grown in a minimal ...
... 1.6.1.19) activity was determined with a colorimetric reaction according to Yonaha and Toyama (1980). Glutamate decarboxylase (GAD, EC 4.1.1.15) activity was measured by the method of Fonda (1985) as modified by Vézina et al. (1988). The bacteria used to measure GAD activity were grown in a minimal ...
DNA Diversity in Sex-Linked and Autosomal Genes of the Plant
... those of S. latifolia and S. dioica from the SlX1 and SlY1 sequences, the total number of sites in this analysis was 1,012 nt. To estimate the recombination statistic, CHud (Hudson 1987), and for the FS neutrality tests (Fu 1997), we also used DNAsp, version 3.5, and P values for the FS neutrality t ...
... those of S. latifolia and S. dioica from the SlX1 and SlY1 sequences, the total number of sites in this analysis was 1,012 nt. To estimate the recombination statistic, CHud (Hudson 1987), and for the FS neutrality tests (Fu 1997), we also used DNAsp, version 3.5, and P values for the FS neutrality t ...
W0=2, a stable aneuploid derivative of Candida
... Rustchenko-Bulgac, 1991). While the major part of this variation is in chromosome R and is due probably to unequal crossing over in the ribosomal DNA repeats, new chromosomal bands frequently appear in both the large and the small size ranges. Many of these are due to translocations (Thrash-Bingham ...
... Rustchenko-Bulgac, 1991). While the major part of this variation is in chromosome R and is due probably to unequal crossing over in the ribosomal DNA repeats, new chromosomal bands frequently appear in both the large and the small size ranges. Many of these are due to translocations (Thrash-Bingham ...
Potato chromosomes IX and XI carry genes for resistance to potato
... Swie_zyński et al. 1981; Mietkiewska 1994). Microsatellites, also called simple sequence repeats (SSRs), are an abundant and dispersed class of repetitive DNA throughout most eukaryotic genomes (Morgante and Olivieri 1993). However, the development of SSR markers for genetic mapping is still relati ...
... Swie_zyński et al. 1981; Mietkiewska 1994). Microsatellites, also called simple sequence repeats (SSRs), are an abundant and dispersed class of repetitive DNA throughout most eukaryotic genomes (Morgante and Olivieri 1993). However, the development of SSR markers for genetic mapping is still relati ...
Molecular Diagnostics 21
... The concentration of denaturant such as formamide in the buffer. The nature of the probe sequence can also impinge on the level of stringency. ...
... The concentration of denaturant such as formamide in the buffer. The nature of the probe sequence can also impinge on the level of stringency. ...
Epigenetic Mediation of Environmental Influences in Major Psychotic
... Environmental mediation of the epigenome can provide a mechanism for the gene-environment interactions currently being uncovered in psychiatric disorders, including psychosis.17,18 The pathogenic effect of a polymorphism associated with disrupted gene function is likely to be dependent upon the degr ...
... Environmental mediation of the epigenome can provide a mechanism for the gene-environment interactions currently being uncovered in psychiatric disorders, including psychosis.17,18 The pathogenic effect of a polymorphism associated with disrupted gene function is likely to be dependent upon the degr ...
Structural Studies on the Dosage Compensation Complex from
... chromosomes. In the XY system, females are homogametic (XX), whereas males are heterogametic (XY) (Charlesworth, 1996). While Y chromosomes are largely heterochromatic and contain few genes, the unequal distribution of X chromosomes would normally result in dramatic changes in gene dosage that could ...
... chromosomes. In the XY system, females are homogametic (XX), whereas males are heterogametic (XY) (Charlesworth, 1996). While Y chromosomes are largely heterochromatic and contain few genes, the unequal distribution of X chromosomes would normally result in dramatic changes in gene dosage that could ...
Cloning and analysis of the mobile element gypsy from D. virilis
... comparable to that of the 'usual' genes suggests that this element may have appeared in this species by horizontal transmission and has not yet changed significantly according to the predicted rate of divergence for an active element. Recently we obtained evidence for the horizontal transmission of ...
... comparable to that of the 'usual' genes suggests that this element may have appeared in this species by horizontal transmission and has not yet changed significantly according to the predicted rate of divergence for an active element. Recently we obtained evidence for the horizontal transmission of ...
Assessing Methods of Detecting Osteogenesis Imperfecta.
... The collagen and genetic samples were obtained from skin fibroblasts from a young boy suspected of having OI, as this is far less invasive than obtaining collagen from placental or amniotic cells. Protein analysis of collagen using PAGE is basically a matter of differentiating a control sample of no ...
... The collagen and genetic samples were obtained from skin fibroblasts from a young boy suspected of having OI, as this is far less invasive than obtaining collagen from placental or amniotic cells. Protein analysis of collagen using PAGE is basically a matter of differentiating a control sample of no ...