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1) Lecture notes: effects of bile salts on cholesterol metabolism
1) Lecture notes: effects of bile salts on cholesterol metabolism

... The mechanism of how a gene is switched on is very complicated and important. It usually requires special proteins that bind to the REGULATORY ELEMENT of the gene. These proteins are usually called TRANSCRIPTION FACTORS. We will study these proteins in detail, for the March and April lectures conti ...
Nucleic Acid Chemistry
Nucleic Acid Chemistry

... • Leading strand – 3’ end of template – As opens up, DNA polymerase binds – Makes new DNA 5’ - 3’ • Same direction as opening of helix ...
DNA Replication - Gadjah Mada University
DNA Replication - Gadjah Mada University

...  Only some of the genes in a cell are active at any given time, and activity also varies by tissue type and developmental stage.  Regulation of gene expression is not completely understood, but it has been shown to involve an array of controlling signals. a. Jacob and Monod (1961) proposed the ope ...
Regulation of Gene Expression
Regulation of Gene Expression

Lecture 7 - School of Science and Technology
Lecture 7 - School of Science and Technology

... • As size of genomes varies dramatically from 10,000 bp for simple viruses up to several billion bp in higher animals and plants, the number of sequences covering the whole genome also varies very significantly 10 – 106. • DNA fragments presented in DB have not only very different lengths but also d ...
Working with Data The Hershey–Chase Experiment
Working with Data The Hershey–Chase Experiment

... either 32P-labeled DNA or 35S-labeled proteins were allowed to infect bacteria. Following infection, the cultures were agitated in a blender to detach viruses from the bacterial cells and then centrifuged. Bacterial cells formed a pellet at the bottom, while the viral particles remained in the super ...
Biology_ch_11_genetics - Miami Beach Senior High School
Biology_ch_11_genetics - Miami Beach Senior High School

... is a double-stranded molecule .  The shape of the DNA molecule is a double-helix (think of a spiral staircase or like a twisted ladder).  The sides of the ladder are composed of alternating sugars (deoxyribose) and ...
Microbial Genetics
Microbial Genetics

... • Plasmids: self replicating circular molecules of NDA • Transposes: small segments of DNA that can move into different parts of the genome. • Can these have an effect on Evolution? ...
Lecture 17 Protein synthesis pp101-110
Lecture 17 Protein synthesis pp101-110

... • RNA Polymerase, An enzyme that oversees the synthesis of RNA Unwinds the DNA template (17 base pair at a time) ...
Proposal for 431 531 - Oregon State University
Proposal for 431 531 - Oregon State University

... In 1986, I was able to do a reasonable job of presenting the principles of plant genetics to an undergraduate audience with little or no background in the subject. I was able to offer sufficient additional material to justify offering the course for graduate credit, particularly for students new to ...
Proposal for 431 531 - Oregon State University
Proposal for 431 531 - Oregon State University

... In 1986, I was able to do a reasonable job of presenting the principles of plant genetics to an undergraduate audience with little or no background in the subject. I was able to offer sufficient additional material to justify offering the course for graduate credit, particularly for students new to ...
Modeling DNA Sequenc..
Modeling DNA Sequenc..

... All major processes in animal development are driven forward by regulatory genes, i.e. genes that express transcription factors Development events are not discrete and the regulatory networks that control development are often connected to other networks that control prior and surrounding processes ...
DNA  1. Evidence for DNA as the genetic material.
DNA 1. Evidence for DNA as the genetic material.

... Remember that DNA polymerase only works in the 5’ to 3’ direction and that it must attach each new nucleotide to a previous nucleotide. At the ends of the chromosomes this is not possible (because there is not a previous nucleotide) on the lagging strand. ii. Each time the cell divides, one strand o ...
Exclusive Highly-Specific Kits and Antibodies for DNA
Exclusive Highly-Specific Kits and Antibodies for DNA

... pathway to demethylate DNA involving a repair mechanism converting hmC to C and, as such open up entirely new perspectives in epigenetic studies. Since its discovery in neuronal Purkinje, granule and ES cells, studies of this new modified DNA base have been limited by the lack of high quality, valid ...
Genetic Transformation computer exercise v02 r01
Genetic Transformation computer exercise v02 r01

... an algorithm (a step-by-step procedure) to compare the order of nucleotide bases in the sequences and then lines them up so that the number of identical bases is maximized. The alignment program will point out those bases that are identical (indicated by an asterisk - ), those that are similar (:), ...
Biology Spring Semester Review
Biology Spring Semester Review

... 3. Predict the genotypes of parents based on the phenotype of the offspring. 4. A homozygous black and a homozygous white dog are crossed. Describe the phenotype of the offspring if the mode of inheritance is: complete dominance, incomplete dominance, and codominance. 5. Be able to use a Punnett Squ ...
Recombinant DNA Technology Biotechnology
Recombinant DNA Technology Biotechnology

... You have transformed bacteria with the plasmid containing the human insulin gene. Only 10% of the bacteria take up the recombinant DNA molecule. Predit which petri plate will have more bacteria growth, the one + ampicillin or the one – ...
of the protein - Duplin County Schools
of the protein - Duplin County Schools

... your DNA. The section of DNA that codes for one protein is called a gene A gene is a section of DNA that determines the sequence of amino acids in a protein. Therefore, the gene determines the shape and therefore, the function of the protein it codes for. ...
Nucleoside Phosphoramidate Monoesters: Potential
Nucleoside Phosphoramidate Monoesters: Potential

... Formation of RNA Polymerase II pre-initiation complex ...
Name: Date: Transcription and Translation Worksheet – ANSWER
Name: Date: Transcription and Translation Worksheet – ANSWER

... 6) If a substitution occurred to the 6th base in the DNA template strand, such that cytosine was changed to thymine, would the final protein change? Why? No. Initially, the DNA strand had the triplet TTC – this created the mRNA codon AAG. If we change the template to TTT, the new codon would be AAA. ...
Regulation of Gene Expression Outline Objectives are first and
Regulation of Gene Expression Outline Objectives are first and

... 2. Methylation + Acetylation = gene expression ( usually lower though) 3. No methylation + Acetylation = gene expression which is higher than number 2 . Obj 3: Explain Alternative Splicing Introns -spliced out of the primary RNA transcript before they can be translated into protein Variant mRNAs-gen ...
5 Conclusion - Duke Computer Science
5 Conclusion - Duke Computer Science

1 - cellbiochem.ca
1 - cellbiochem.ca

... Type II restriction enzymes Blunt end-generating RE (e.g. EcoR V): EcoR V generates 5’ phosphate and 3’ OH ends ...
CHS H Bio Final Exam Review Sheet
CHS H Bio Final Exam Review Sheet

... What does DNA store in a cell? If you were looking at a nucleotide, how could you tell if the nitrogen base was a purine or a pyrimidine? What is the base pairing rule? If a DNA sample had 20% Guanine, what would the percents of the other three types of nucleotides be? What does semi-conservative me ...
Biology 321 Spring 2011 Answers to Assignment Set #5
Biology 321 Spring 2011 Answers to Assignment Set #5

... e. It is in the first codon position so likely toe be a neutral missense  Problem 19 Answer not available just yet. Will post it on FRiday  Problem 20 a. Review definition of polymorphism in earlier lecture b. silent or same sense mutation c. Neutral missense mutation (note legend at bottom of tab ...
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Non-coding DNA

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