Adaptation of Sucrose Metabolism in the Escherichia coli Wild
... sucrose on MacConkey sucrose (1%) indicator plates. Two different Csc-positive clones, one from each subcloning experiment, were further analyzed. A 5.5-kb EcoRI and a 9.3-kb PvuII insert in pSU18 were identified, and the plasmids were designated pJBL101 and pJBL103, respectively (Fig. 1). JM109/pJB ...
... sucrose on MacConkey sucrose (1%) indicator plates. Two different Csc-positive clones, one from each subcloning experiment, were further analyzed. A 5.5-kb EcoRI and a 9.3-kb PvuII insert in pSU18 were identified, and the plasmids were designated pJBL101 and pJBL103, respectively (Fig. 1). JM109/pJB ...
Boundary elements and nuclear organization
... possess gene regulatory properties (Forrester et al., 1999; Fernandez et al., 2001). Recently, physical clustering of co-expressed genes was found prevalent throughout eukaryotic genomes, including yeast, Drosophila and humans, reinforcing the notion of transcriptional compartmentalization through p ...
... possess gene regulatory properties (Forrester et al., 1999; Fernandez et al., 2001). Recently, physical clustering of co-expressed genes was found prevalent throughout eukaryotic genomes, including yeast, Drosophila and humans, reinforcing the notion of transcriptional compartmentalization through p ...
Multiple Mechanisms Contribute to Lateral Transfer of an
... ATCC 27551 that encodes genes for organophosphate degradation (opd), revealed the existence of a sitespecific integrase (int) gene with an attachment site attP, typically seen in integrative mobilizable elements (IME). In agreement with this sequence information, site-specific recombination was observ ...
... ATCC 27551 that encodes genes for organophosphate degradation (opd), revealed the existence of a sitespecific integrase (int) gene with an attachment site attP, typically seen in integrative mobilizable elements (IME). In agreement with this sequence information, site-specific recombination was observ ...
巴西橡胶Pto类抗病同源序列的克隆与系统发育重建
... the predicted amino acid sequences of the seven hevea Pto-RGCs and the corresponding region of the tomato Pto protein (Fig. 1). The alignment revealed that several features of the Pto protein are highly conserved in the hevea Pto-RGCs such as the STK subdomains internal to the degenerate primer sequ ...
... the predicted amino acid sequences of the seven hevea Pto-RGCs and the corresponding region of the tomato Pto protein (Fig. 1). The alignment revealed that several features of the Pto protein are highly conserved in the hevea Pto-RGCs such as the STK subdomains internal to the degenerate primer sequ ...
entire lesson plan PDF
... Biotechnology can be defined as the controlled and deliberate manipulation of biological systems (whether living cells or cell components) for the efficient manufacture or processing of useful products. The fact that living organisms have evolved such an enormous spectrum of biological capabilities ...
... Biotechnology can be defined as the controlled and deliberate manipulation of biological systems (whether living cells or cell components) for the efficient manufacture or processing of useful products. The fact that living organisms have evolved such an enormous spectrum of biological capabilities ...
BioACTS Quarter THREE
... Yes, because it saves time, energy, and resources; new perspectives on shared data can expand and /or guide additional research. Avery repeated Griffith’s research experiment on transformation. Rosalind Franklin’s X-ray diffraction data helped Watson and Crick to make the first correct 3-D model of ...
... Yes, because it saves time, energy, and resources; new perspectives on shared data can expand and /or guide additional research. Avery repeated Griffith’s research experiment on transformation. Rosalind Franklin’s X-ray diffraction data helped Watson and Crick to make the first correct 3-D model of ...
2- pcr primer design and reaction optimisation
... the likelihood of annealing to sequences other than the chosen target is very low. For example, there is a 1⁄4 chance (4-1) of finding an A, G, C or T in any given DNA sequence; there is a 1/16 chance (4-2) of finding any dinucleotide sequence (eg. AG); a 1/256 chance of finding a given 4-base seque ...
... the likelihood of annealing to sequences other than the chosen target is very low. For example, there is a 1⁄4 chance (4-1) of finding an A, G, C or T in any given DNA sequence; there is a 1/16 chance (4-2) of finding any dinucleotide sequence (eg. AG); a 1/256 chance of finding a given 4-base seque ...
... invention, there is provided a method of and products for other cells, such that these other cells are now actively diagnosing a prostate disorder by determining an altered transcribing and expressing the PSR genes and the mRNA is level of PSR protein in a biological sample derived from a present. I ...
Identification of markers tightly linked to tomato yellow
... The tomato (Solanum lycopersicon) is an economically important species of the Solanaceae family, and it is cultivated all over the world for human consumption. Recently, tomato crops have often been infected by tomato yellow leaf curl virus (TYLCV), which causes significant yield losses in tomato (S ...
... The tomato (Solanum lycopersicon) is an economically important species of the Solanaceae family, and it is cultivated all over the world for human consumption. Recently, tomato crops have often been infected by tomato yellow leaf curl virus (TYLCV), which causes significant yield losses in tomato (S ...
NMSU IBC rDNA Worksheet for PI - Office of Research Compliance
... Caution - Special care should be used in the evaluation of containment conditions for some experiments with transgenic animals. For example, such experiments might lead to the creation of novel mechanisms or increased transmission of a recombinant pathogen or production of undesirable traits in the ...
... Caution - Special care should be used in the evaluation of containment conditions for some experiments with transgenic animals. For example, such experiments might lead to the creation of novel mechanisms or increased transmission of a recombinant pathogen or production of undesirable traits in the ...
Genetics - Muscular Dystrophy Canada
... given protein at a specific time and in specific cells. Each gene codes for a particular protein that will have its own responsibilities in cells. One gene might make a protein all the time, in all cells; another gene might make a protein in liver cells for a short period of time. The engine of a ge ...
... given protein at a specific time and in specific cells. Each gene codes for a particular protein that will have its own responsibilities in cells. One gene might make a protein all the time, in all cells; another gene might make a protein in liver cells for a short period of time. The engine of a ge ...
gmod-arthrobase-07dec - IUBio Archive for Biology
... TIFF (Uncompressed) decompressor are needed to see this picture. ...
... TIFF (Uncompressed) decompressor are needed to see this picture. ...
lntraclonal mating in Trypanosoma brucei is
... matings. In the three successful out-crosses, hybrids were found among trypanosomes derived from the salivary glands but not midguts of individual flies. This brings the total number of crosses in which hybrids have been found only in the salivary glands to five and strengthens the view that the sal ...
... matings. In the three successful out-crosses, hybrids were found among trypanosomes derived from the salivary glands but not midguts of individual flies. This brings the total number of crosses in which hybrids have been found only in the salivary glands to five and strengthens the view that the sal ...
Unit IX: Identification of a Gram
... Another approach to identify bacteria is based on the Restriction Fragment Length Polymorphism (RFLP) of 16SrRNA genes (16SrDNA); its results in a type of DNA-fingerprint. The secondary structure of the 16SrRNA (Figure 27.1) is highly conserved and functions well as a molecular chronometer. For this ...
... Another approach to identify bacteria is based on the Restriction Fragment Length Polymorphism (RFLP) of 16SrRNA genes (16SrDNA); its results in a type of DNA-fingerprint. The secondary structure of the 16SrRNA (Figure 27.1) is highly conserved and functions well as a molecular chronometer. For this ...
TILLING. Traditional Mutagenesis Meets
... A key advantage of high-throughput TILLING over competing methods is that the approximate position of each detected mutation is inferred from the size of the fragment, which greatly facilitates subsequent sequencing. Furthermore, the double-end labeling strategy provides confirmation within the pool ...
... A key advantage of high-throughput TILLING over competing methods is that the approximate position of each detected mutation is inferred from the size of the fragment, which greatly facilitates subsequent sequencing. Furthermore, the double-end labeling strategy provides confirmation within the pool ...
Canadian Journal of Microbiology
... (Adhikari et al. 2001; Zinniel et al. 2002; Elvira-Recuenco and van Vuurde 2000). Owing to insufficient knowledge about the growth requirements of many microorgansims as well as the fact that cells may enter a viable but not culturable status (Tholozan et al. 1999), culture-dependent methods do not ...
... (Adhikari et al. 2001; Zinniel et al. 2002; Elvira-Recuenco and van Vuurde 2000). Owing to insufficient knowledge about the growth requirements of many microorgansims as well as the fact that cells may enter a viable but not culturable status (Tholozan et al. 1999), culture-dependent methods do not ...
What`s in a Genotype? - CEUR Workshop Proceedings
... down into reference and variant components, the genomic variation complement can be further decomposed into one or more variant single locus complements, representing the set of all complementary loci where at least one variant exists (Figure 1B). This complement is typically a pair of sequences for ...
... down into reference and variant components, the genomic variation complement can be further decomposed into one or more variant single locus complements, representing the set of all complementary loci where at least one variant exists (Figure 1B). This complement is typically a pair of sequences for ...
Human Ig heavy chain CDR3 regions in adult
... diversity is illustrated by the fact that this region forms the center of the antigen binding site and provides essential residues for antigen binding (2,3,18–22). Given the central location of the HCDR3 within the antigen-binding pocket, major changes in HCDR3 length, sequence and/or bulk are thoug ...
... diversity is illustrated by the fact that this region forms the center of the antigen binding site and provides essential residues for antigen binding (2,3,18–22). Given the central location of the HCDR3 within the antigen-binding pocket, major changes in HCDR3 length, sequence and/or bulk are thoug ...
MAMMALS THAT BREAK THE RULES:Genetics of Marsupials and
... follows its own rules of recombination. Linkage mapping in a dasyurid species showed that recombination rates are strikingly different in male and female marsupials. Rather than the minor male deficit in recombination standard for eutherians, marsupial females have far less recombination than males ...
... follows its own rules of recombination. Linkage mapping in a dasyurid species showed that recombination rates are strikingly different in male and female marsupials. Rather than the minor male deficit in recombination standard for eutherians, marsupial females have far less recombination than males ...
Molecualr Biology and Evolution
... Parsimony and some distance analyses of the nifK amino acid sequences provide support for vertical descent of nzyK, but other distance trees provide support for the lateral transfer of the gene. Bootstrap support was found for both hypotheses in all trees; the nifK data do not definitively favor one ...
... Parsimony and some distance analyses of the nifK amino acid sequences provide support for vertical descent of nzyK, but other distance trees provide support for the lateral transfer of the gene. Bootstrap support was found for both hypotheses in all trees; the nifK data do not definitively favor one ...
Garrett-Engele* C. M., Siegal*, M. L., Manoli, D. S., Williams, B. C., Li. H., and Baker, B. S.
... Comparisons of the phenotypes of her, dsx double mutant flies with those of flies that are mutant at just one of these genes showed that her and dsx act independently to regulate some aspects of sexual differentiation and function interdependently to control other aspects of sexual differentiation i ...
... Comparisons of the phenotypes of her, dsx double mutant flies with those of flies that are mutant at just one of these genes showed that her and dsx act independently to regulate some aspects of sexual differentiation and function interdependently to control other aspects of sexual differentiation i ...
Molecular Phylogenetics Basis of Phylogenetics
... ¾ a phylogeny describe the historical relationships among lineages or organisms or their parts (genes) ¾ using trees to test hypotheses ...
... ¾ a phylogeny describe the historical relationships among lineages or organisms or their parts (genes) ¾ using trees to test hypotheses ...