New Developments in the Embryology Laboratory
... nucleus (small nuclear RNAs)and in the nucleolus (small nucleolar RNAs) are important components of transcriptomics • Proportion of mRNAs in a transcriptome account for only 1-2% of total RNA content in somatic cells ...
... nucleus (small nuclear RNAs)and in the nucleolus (small nucleolar RNAs) are important components of transcriptomics • Proportion of mRNAs in a transcriptome account for only 1-2% of total RNA content in somatic cells ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... P22pro-I and P22pro-3 are specialized transducing derivatives of phage P22 that carry the proA and proB genes of Salmonella typhimurium. These genes lie immediately adjacent to the prophage attachment site on the bacterial chromosome. By examining DNA heteroduplexes in the electron microscope, we fo ...
... P22pro-I and P22pro-3 are specialized transducing derivatives of phage P22 that carry the proA and proB genes of Salmonella typhimurium. These genes lie immediately adjacent to the prophage attachment site on the bacterial chromosome. By examining DNA heteroduplexes in the electron microscope, we fo ...
BLOTTING.142
... the basis of size by electrophoresis. The large fragments move more slowly than ...
... the basis of size by electrophoresis. The large fragments move more slowly than ...
DNA
... More Terminology • The genome is an organism’s complete set of DNA • a bacteria contains about 600,000 DNA base pairs • human and mouse genomes have some 3 billion ...
... More Terminology • The genome is an organism’s complete set of DNA • a bacteria contains about 600,000 DNA base pairs • human and mouse genomes have some 3 billion ...
93134006蔡家妮 期中作業二
... Asia. The 57·3 kb Vibrio pathogenicity island-2 (VPI-2) is confined predominantly to toxigenic V. cholerae O1 and O139 serogroup isolates and encodes 52 ORFs (VC1758 to VC1809), which include homologues of an integrase (VC1758), a restriction modification system, a sialic acid metabolism gene cluste ...
... Asia. The 57·3 kb Vibrio pathogenicity island-2 (VPI-2) is confined predominantly to toxigenic V. cholerae O1 and O139 serogroup isolates and encodes 52 ORFs (VC1758 to VC1809), which include homologues of an integrase (VC1758), a restriction modification system, a sialic acid metabolism gene cluste ...
BMC Evolutionary Biology D- and L-lactate dehydrogenases during invertebrate evolution
... the L-specific NAD-dependent dehydrogenases, while D(+)-LDHs belong to the D-isomer specific 2-hydroxy acid dehydrogenases and the FAD-binding oxidoreductase/transferase type 4 family. L-LDH has been among the most studied enzyme families, but very little is known about the structure, function, and ...
... the L-specific NAD-dependent dehydrogenases, while D(+)-LDHs belong to the D-isomer specific 2-hydroxy acid dehydrogenases and the FAD-binding oxidoreductase/transferase type 4 family. L-LDH has been among the most studied enzyme families, but very little is known about the structure, function, and ...
Mutation Analysis of the Ornithine Transcarbamylase (OTC) Gene in
... two known and three novel mutations of the OTC gene in five Japanese patients including two neonatal-onset, one late-onset, and two symptomatic female patients. Known nonsense mutations (c.578G>A and c.421C>T) were detected in a neonatal-onset male and a symptomatic female patient, respectively. Mut ...
... two known and three novel mutations of the OTC gene in five Japanese patients including two neonatal-onset, one late-onset, and two symptomatic female patients. Known nonsense mutations (c.578G>A and c.421C>T) were detected in a neonatal-onset male and a symptomatic female patient, respectively. Mut ...
Gel Electrophoresis
... needed for stretching and reorientation, larger molecules have less time left for migration in the electric field. In PFGE, the resulting electrophoretic mobilities depend on the pulse time: DNA molecules with fragment sizes up to about 10 megabases (Mb) can be resolved. Pulse times of 1 s to 90 min ...
... needed for stretching and reorientation, larger molecules have less time left for migration in the electric field. In PFGE, the resulting electrophoretic mobilities depend on the pulse time: DNA molecules with fragment sizes up to about 10 megabases (Mb) can be resolved. Pulse times of 1 s to 90 min ...
"big IB objectives"-use the blank paper technique
... 3.4.1 – explain DNA replication in terms of unwinding the double helix and separation of the strands by helicase, followed by formation of the new complementary strands by DNA polymerase 3.4.2 – explain the significance of complementary base pairing in the conservation of the base sequence of DNA 3 ...
... 3.4.1 – explain DNA replication in terms of unwinding the double helix and separation of the strands by helicase, followed by formation of the new complementary strands by DNA polymerase 3.4.2 – explain the significance of complementary base pairing in the conservation of the base sequence of DNA 3 ...
Structure of a Plasmodium yoelii gene
... Isolation and sequence of a P. yoelii clone Screenings with M15 of 2xlO 4 plaques from a genomic library AZAP, yielded 24 positive phage clones with different signal intensities, of which the inserts of four phage clones (denoted YEL3, 5, 6 and 11) were excised and subcloned into pBluescript. They w ...
... Isolation and sequence of a P. yoelii clone Screenings with M15 of 2xlO 4 plaques from a genomic library AZAP, yielded 24 positive phage clones with different signal intensities, of which the inserts of four phage clones (denoted YEL3, 5, 6 and 11) were excised and subcloned into pBluescript. They w ...
Application Note: Targeted sequencing and chromosomal haplotype
... are tens of kilobases in length. The Targeted Locus Amplification (TLA) Technology1 from Cergentis enables the targeted, hypothesis-neutral, amplification of any genomic locus of interest over 50 kb using just one primer pair complementary to a short locusspecific sequence. TLA is a strategy to sele ...
... are tens of kilobases in length. The Targeted Locus Amplification (TLA) Technology1 from Cergentis enables the targeted, hypothesis-neutral, amplification of any genomic locus of interest over 50 kb using just one primer pair complementary to a short locusspecific sequence. TLA is a strategy to sele ...
Complete Elimination of Endosymbiotic Algae from Paramecium
... 256 M. Tanaka et al. single species or not. We have previously cloned endosymbiotic algae from P. bursaria and characterization of each clone was carried out (Nishihara et al. 1998). On the other hand, we have reported a new technique to produce algae-free paramecia by treating green paramecia with ...
... 256 M. Tanaka et al. single species or not. We have previously cloned endosymbiotic algae from P. bursaria and characterization of each clone was carried out (Nishihara et al. 1998). On the other hand, we have reported a new technique to produce algae-free paramecia by treating green paramecia with ...
Specialized adaptation of a lactic acid bacterium to the milk
... development [11–16], and several of the clustered regularly interspaced short palindromic repeats (CRISPR) regions not present in both CNRZ1066 and LMG 18311 that are involved in bacteriophage defense [17, 18]. Interestingly, long before even the notion of a genome had even been conceived, S. ther ...
... development [11–16], and several of the clustered regularly interspaced short palindromic repeats (CRISPR) regions not present in both CNRZ1066 and LMG 18311 that are involved in bacteriophage defense [17, 18]. Interestingly, long before even the notion of a genome had even been conceived, S. ther ...
Properties and sequence of the coenzyme B12
... K. pneumoniae and K. oxytoca that this gene product is not required for dehydratase activity [4,18,23]. However, the gene product of K. oxytoca is suspected to be involved in reactivation of glycerol-inactivated diol dehydratase or the enzyme-cyanocobalamin complex [24]. The proteins encoded by the ...
... K. pneumoniae and K. oxytoca that this gene product is not required for dehydratase activity [4,18,23]. However, the gene product of K. oxytoca is suspected to be involved in reactivation of glycerol-inactivated diol dehydratase or the enzyme-cyanocobalamin complex [24]. The proteins encoded by the ...
Insights into three whole-genome duplications gleaned
... (Figure 1), this complex pattern implies multiple changes of the GC content within the different lineages of Paramecium. Such changes could reflect a difference in the mutation spectrum or in processes such as biased gene conversion. In the future, sequencing the genomes of more P. aurelia species a ...
... (Figure 1), this complex pattern implies multiple changes of the GC content within the different lineages of Paramecium. Such changes could reflect a difference in the mutation spectrum or in processes such as biased gene conversion. In the future, sequencing the genomes of more P. aurelia species a ...
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... resistance genes that are effective against the leaf, stem and yellow rust pathogens, have been designated. The gene of importance for this study is leaf rust resistance gene Lr37, which maps to chromosome 2AS (Bariana & McIntosh, 1993). The gene is effective against leaf rust pathotypes UVPrt2 and ...
... resistance genes that are effective against the leaf, stem and yellow rust pathogens, have been designated. The gene of importance for this study is leaf rust resistance gene Lr37, which maps to chromosome 2AS (Bariana & McIntosh, 1993). The gene is effective against leaf rust pathotypes UVPrt2 and ...
FEBS Letters
... smaller subunit o f the hydrogenase dimer, sequence identity to the A. variabilis gene product 61% on an amino acid basis) and hoxU (coding for the smaller subunit of the diaphorase part, sequence identity to A. variabilis = 63%). However, hoxF (coding for the larger subunit of the diaphorase part) ...
... smaller subunit o f the hydrogenase dimer, sequence identity to the A. variabilis gene product 61% on an amino acid basis) and hoxU (coding for the smaller subunit of the diaphorase part, sequence identity to A. variabilis = 63%). However, hoxF (coding for the larger subunit of the diaphorase part) ...
Analysis of the histone H3 gene family in Arabidopsis and
... Plant Molecular Biology and Biotechnology laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Institute of Land and Food Resources, The University of Melbourne, Parkville, Victoria 3010, Australia ...
... Plant Molecular Biology and Biotechnology laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Institute of Land and Food Resources, The University of Melbourne, Parkville, Victoria 3010, Australia ...
Document
... along to future generations, and express that information as it carries out all the processes of life. The major steps involved in handling genetic information are illustrated by the central dogma of molecular biology (Figure I-1-1). Genetic information is stored in the base sequence of DNA molecule ...
... along to future generations, and express that information as it carries out all the processes of life. The major steps involved in handling genetic information are illustrated by the central dogma of molecular biology (Figure I-1-1). Genetic information is stored in the base sequence of DNA molecule ...
Recent retrotransposition events have not affected
... expression in five tissues (data from Khaitovich et al, Science, 2005) Expression divergence is the Euclidean distance between expression levels in one or more tissues ...
... expression in five tissues (data from Khaitovich et al, Science, 2005) Expression divergence is the Euclidean distance between expression levels in one or more tissues ...
