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1 LIFE SCIENCES PAPER 1 SEPTEMBER 2008 LIFE SCIENCES
1 LIFE SCIENCES PAPER 1 SEPTEMBER 2008 LIFE SCIENCES

... The sequence of bases in a molecule of DNA codes for proteins. Different sequences of bases code for different proteins. Although the base sequence AGT codes for serine, other sequences may also code for this same amino acid. There are four sequences which code for the amino acid glycine; these are ...
Chapter 13
Chapter 13

... bands (one RFLP from each chromosome). • When comparing the DNA fragment patterns of two or more specimens, one merely looks for a match between the band sets. • A high degree of discrimination can be achieved by using a number of different probes and combining their frequencies. CRIMINALISTICS An I ...
Activity #5b. Plasmid DNA Isolation, Restriction Enzyme Digestion
Activity #5b. Plasmid DNA Isolation, Restriction Enzyme Digestion

... of specific base sequences. However, these do not provide information on the function of the DNA. To examine the function of a specific DNA, it must be studied in vivo (in life). What is the function of DNA? DNA contains genes encoding proteins (usually) that do something for the organism. Two genes ...
DNA
DNA

... bands (one RFLP from each chromosome). • When comparing the DNA fragment patterns of two or more specimens, one merely looks for a match between the band sets. • A high degree of discrimination can be achieved by using a number of different probes and combining their frequencies. CRIMINALISTICS An I ...
Αρχές Ιατρικής Γενετικής - e
Αρχές Ιατρικής Γενετικής - e

... syndrome. Note his coarse facial features, crouched stance, thickened digits, and protuberant abdomen. B, Transgenic mice with a targeted disruption of α-liduronidase. Progressive coarsening of the face is apparent as 8-week-old mice (left) grow to become 52-week-old mice ...
Full Text
Full Text

... Early embryos of the crustacean Artemia franciscana (brine shrimp) can get into cryptobiosis under adverse environmental conditions. Under these circumstances, the embryo arrests all metabolic activities, gets dehydrated and is surrounded by a hard shell. These cysts are viable for long periods and ...
Basic molecular genetics for epidemiologists
Basic molecular genetics for epidemiologists

... general increase in the number of epidemiological research articles that apply basic science methods in their studies, resulting in what is known as both molecular and genetic epidemiology, is evident. Actually, genetics has come into the epidemiological scene with plenty of new sophisticated concep ...
Intraspecies variation in bacterial genomes: the need for a
Intraspecies variation in bacterial genomes: the need for a

... phages/cryptic prophages in Escherichia coli K-12 that are introduced into the chromosome on trans(Ref. 1). Some are simply parasitic and not an integral posons and then moved between strains. The part of the genome, but others carry genes that make chromosome can then be cut into large-sized fragth ...
Chapter 4 DNA, RNA, and the Flow of Genetic Information
Chapter 4 DNA, RNA, and the Flow of Genetic Information

... Ans: The genetic code has no punctuation. The sequence of bases is read sequentially from a fixed starting point codon, AUG. After the reading begins, every set of three bases codes for one amino acid in the protein sequence. If an insertion or a deletion occurs, there can be a completely different ...
C:\BOB\HSC\Exams 05\Supps\Biology 3201 August 2005.wpd
C:\BOB\HSC\Exams 05\Supps\Biology 3201 August 2005.wpd

... In pea plants, spherical seed shape (S) is dominant to dented seed shape (s) and yellow seeds (Y) are dominant to green seeds (y). If two pea plants, each heterozygous for both traits, are crossed, what is the probability of the offspring ...
Document
Document

... produces desired product • Mutation: Mutagens cause mutations that might result in a microbe with a desirable trait • Site-directed mutagenesis: Change a specific DNA code to change a protein • Select and culture microbe with the desired mutation ...
LIGATION AND TRANSFORMATION
LIGATION AND TRANSFORMATION

... (at 3-5 mM) can alleviate some of this inhibition. However, when eluting DNA from gels for use with enzymes an agarose that is made specifically for use with enzymes should be used. Monovalent cations (e.g., Na+and K+) at moderate concentrations (100-200 mM) can also inhibit ligation. However, when ...
1 Lecture 5 Molecular biology Dr. Israa Al
1 Lecture 5 Molecular biology Dr. Israa Al

Homeotic genes - Teacherschoice
Homeotic genes - Teacherschoice

Homeotic genes
Homeotic genes

... Genes that affect embryo development by specifying the character of a body segment. The classic example is Antennapedia, a gene that, when mutated, causes a fruitfly to grow a leg in place of antenna. Homeotic genes are homeobox genes that are responsible for segment identity in metazoan organisms. ...
Lecture No - Taleem-E
Lecture No - Taleem-E

... compute a distance between DNA sequences (strings over A,C,G,T, or protein sequences (over an alphabet of 20 amino acids), for various purposes, e.g.: • to find genes or proteins that may have shared functions or properties • to infer family relationships and evolutionary trees over different organi ...
Profiling Complex Communities with Highly Accurate Single
Profiling Complex Communities with Highly Accurate Single

... Determining compositions and functional capabilities of complex populations is often challenging, especially for sequencing technologies with short reads that do not uniquely identify organisms or genes. Long-read sequencing improves the resolution of these mixed communities, but adoption for this a ...
Document
Document

... • The functions of human genes and other DNA regions often are revealed by studying their parallels in nonhumans. – Researchers have learned a great deal about the function of human genes by examining their counterparts in simpler model organisms such as the mouse. ...
Engineering of diffraction-quality crystals of the NF-κB
Engineering of diffraction-quality crystals of the NF-κB

... of human N F - K B P50, 14 mostly charged residues comprising the NLS, are invisible in the electron density maps. Tyr-351 in human N F - K B P50 (Tyr-326 in N F - K B P52) is the last residue involved in secondary structure interactions of the C-terminal Ig-domain (P-strand g). We suspected that an ...
Final Exam Study Guide 2015
Final Exam Study Guide 2015

... ◦ Know how and why dominant and recessive disorders have behaved differently over time ◦ Understand that genetic disorders affect phenotype by altering a gene’s protein product ...
Blueprint of Life
Blueprint of Life

... told to prey on the 'worms' in the field (collect as many toothpicks as they can) in a given time. After 3 minutes, the 'stick-birds' are driven from the field by the 'farmer' (teacher). They escape back to the classroom. Tally and compare the numbers of green and cream toothpicks recovered. Calcula ...
Blue Box PowerPoint Presentation Template
Blue Box PowerPoint Presentation Template

... a few predisposing genes Works well even when many different mutations or alleles in those few genes increase risk for the disease Requires no hypothesis about the nature of the defect in the disorder usually requires a model of the pattern of inheritance ...
Recombinant DNA Technology
Recombinant DNA Technology

... • Stable transfection with viral vectors. The virus carries the DNA into the cell more efficiently than any other method. – You need to select the rare cells that have incorporated the DNA using a drug resistance gene as part of the transformation vector. The only cells that survive treatment with t ...
mutations
mutations

... archaebacterial, and eukaryotic sequences. indicate the four clusters where RifR mutations have been identified in E. coli. Mutations that confer RifR in E. coli and M. tuberculosis are indicated directly above (for E. coli) or below (for M. tuberculosis) as follows: D for deletions, V for insertion ...
pEGFP-C1 - Newcastle University Staff Publishing Service
pEGFP-C1 - Newcastle University Staff Publishing Service

... the fusion protein in vivo. The target gene should be cloned into pEGFP-C1 so that it is in frame with the EGFP coding sequences, with no intervening in-frame stop codons. The recombinant EGFP vector can be transfected into mammalian cells using any standard transfection method. If required, stable ...
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Non-coding DNA

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