1Representation and analysis of DNA sequences
... determines excessive computing costs in the case of studies involving feature extraction at the scale of whole chromosomes, multiresolution analysis, comparative genomic analysis, or quantitative variability analysis [20, 21, 22]. Converting the DNA sequences into digital signals [23, 24] opens the ...
... determines excessive computing costs in the case of studies involving feature extraction at the scale of whole chromosomes, multiresolution analysis, comparative genomic analysis, or quantitative variability analysis [20, 21, 22]. Converting the DNA sequences into digital signals [23, 24] opens the ...
The Value of MLPA in Waardenburg Syndrome - MRC
... Since PAX3 was cloned (Baldwin et al., 1992), no other gene has been discovered as an etiology for WS 1 or 3. Point mutations in PAX3 have been identified in more than 90% of affected individuals with WS 1 or 3. In contrast, WS2 is genetically heterogeneous, with only 10–15% of affected individuals ...
... Since PAX3 was cloned (Baldwin et al., 1992), no other gene has been discovered as an etiology for WS 1 or 3. Point mutations in PAX3 have been identified in more than 90% of affected individuals with WS 1 or 3. In contrast, WS2 is genetically heterogeneous, with only 10–15% of affected individuals ...
Bayesian Partition Models for Identifying Expression Quantitative
... Mapping genetic loci for multiple traits simultaneously has also been shown to be more powerful than mapping single traits at a time (Jiang and Zeng, 1995). Although for a known small set of correlated traits, one can conduct QTL mapping for a few principal components (Mangin et al., 1998), this typ ...
... Mapping genetic loci for multiple traits simultaneously has also been shown to be more powerful than mapping single traits at a time (Jiang and Zeng, 1995). Although for a known small set of correlated traits, one can conduct QTL mapping for a few principal components (Mangin et al., 1998), this typ ...
The Co-Evolution of Genes and Culture Pedigrees and the
... 11. Individual 4, Generation IV in Family B is the sister of Individuals 8 and 9 in the question above. What is the probability that Individual 4 is homozygous? 50% Heterozygous? 50% A pedigree chart is a tool that helps illustrate how a trait is inherited from one generation to the next. Below is ...
... 11. Individual 4, Generation IV in Family B is the sister of Individuals 8 and 9 in the question above. What is the probability that Individual 4 is homozygous? 50% Heterozygous? 50% A pedigree chart is a tool that helps illustrate how a trait is inherited from one generation to the next. Below is ...
Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
Week 2. DNA isolation and PCR
... I like to show the PCR video (https://www.youtube.com/watch?v=2KoLnIwoZKU) in the laboratory and provide my own commentary to ensure that students understand the PCR process. As a class, I then ask the students to volunteer the expected sizes of the AS1 and AS2 PCR products they calculated in their ...
... I like to show the PCR video (https://www.youtube.com/watch?v=2KoLnIwoZKU) in the laboratory and provide my own commentary to ensure that students understand the PCR process. As a class, I then ask the students to volunteer the expected sizes of the AS1 and AS2 PCR products they calculated in their ...
Non-destructive DNA extraction methods that yield DNA barcodes in
... species, and voucher specimens (Chen et al., 2010). Thus, by using this mechanism, species, old and new alike, could be barcoded efficiently. DNA extraction of Spiders to Gain Barcodes Since there are about 37,000 identified species of spiders and 40,000 suspected species of spiders, barcoding each ...
... species, and voucher specimens (Chen et al., 2010). Thus, by using this mechanism, species, old and new alike, could be barcoded efficiently. DNA extraction of Spiders to Gain Barcodes Since there are about 37,000 identified species of spiders and 40,000 suspected species of spiders, barcoding each ...
Physiological and Molecular Plant Pathology (2001) 59, 33-43
... protein size (16-19 kDa), amino acid sequence and gene organization. They are closely related to allergens, such as Bet v 1, the major birch pollen allergen [5] and Mal d 1, the major apple allergen [41]. The induction of PR10 genes has been described in several plant species upon pathogen attack or ...
... protein size (16-19 kDa), amino acid sequence and gene organization. They are closely related to allergens, such as Bet v 1, the major birch pollen allergen [5] and Mal d 1, the major apple allergen [41]. The induction of PR10 genes has been described in several plant species upon pathogen attack or ...
Plant centromeres: structure and control Eric J Richards and R Kelly
... to the highly reiterated repeats, there is a variety of other sequences, including low-copy sequences [22,32-35], transposable elements [36] and telomere-similar repeats [22,28,37], found specifically or primarily in centromere regions in various plant species. The growing collection of plant centro ...
... to the highly reiterated repeats, there is a variety of other sequences, including low-copy sequences [22,32-35], transposable elements [36] and telomere-similar repeats [22,28,37], found specifically or primarily in centromere regions in various plant species. The growing collection of plant centro ...
Amplicon metagenomics for elucidating microbial community
... authors argue that 16s rRNA might not be as convenient of a marker since it has variable copy numbers, with some taxa having up to 15 copies of this gene (Kembe et al, 2012). As a consequence, those taxa might be overrepresented in the final set of sequences and that could give rise to biased commun ...
... authors argue that 16s rRNA might not be as convenient of a marker since it has variable copy numbers, with some taxa having up to 15 copies of this gene (Kembe et al, 2012). As a consequence, those taxa might be overrepresented in the final set of sequences and that could give rise to biased commun ...
Analysis of DNA transcription termination sequences of gene coding
... The proteins involved in the synthesis of PHAs are encoded by the pha gene cluster containing two polymerase genes phaC1 and phaC2, a depolymerase gene phaZ and three regulatory genes phaD, phaF and phaI. Even though the key genes engaged in the process of PHAs synthesis are known, there is a lack o ...
... The proteins involved in the synthesis of PHAs are encoded by the pha gene cluster containing two polymerase genes phaC1 and phaC2, a depolymerase gene phaZ and three regulatory genes phaD, phaF and phaI. Even though the key genes engaged in the process of PHAs synthesis are known, there is a lack o ...
PDF - American Society of Mammalogists
... effects may be caused by several mechanisms including sex chromosomes and maternal inheritance of mitochondrial DNA. Recently, a class of mammalian autosomal genes has emerged that shows expression of only 1 parental allele. This phenomenon has been termed ‘‘genomic imprinting.’’ Genomic imprinting ...
... effects may be caused by several mechanisms including sex chromosomes and maternal inheritance of mitochondrial DNA. Recently, a class of mammalian autosomal genes has emerged that shows expression of only 1 parental allele. This phenomenon has been termed ‘‘genomic imprinting.’’ Genomic imprinting ...
ELMER: An R/Bioconductor Tool Inferring Regulatory Element
... capture changes in individual molecular subtypes occurring in 20% or more of the cases. This number can be set arbitrarily as an input to the get.diff.meth function in the ELMER, and should be tuned based on sample sizes in individual studies. The one tailed t-test was used to rule out the null hyp ...
... capture changes in individual molecular subtypes occurring in 20% or more of the cases. This number can be set arbitrarily as an input to the get.diff.meth function in the ELMER, and should be tuned based on sample sizes in individual studies. The one tailed t-test was used to rule out the null hyp ...
University of Groningen DNA-based asymmetric catalysis
... primary coordination sphere provided by the metal complex have the most significant effect on the enantioselectivity, while the fine tuning of the enantioselectivity is governed by the modification of the secondary coordination sphere provided by the protein.27 These two parameters combined should i ...
... primary coordination sphere provided by the metal complex have the most significant effect on the enantioselectivity, while the fine tuning of the enantioselectivity is governed by the modification of the secondary coordination sphere provided by the protein.27 These two parameters combined should i ...
Survey of Conserved Alternative Splicing Events
... proteins regulate AS in a tissue-specific and stress-responsive manner. Pre-mRNAs encoding SR proteins are often alternatively spliced, and these AS events may be important for the regulation of AS events of other pre-mRNAs. In this study, we analyzed AS events of SR proteins in Arabidopsis thaliana ...
... proteins regulate AS in a tissue-specific and stress-responsive manner. Pre-mRNAs encoding SR proteins are often alternatively spliced, and these AS events may be important for the regulation of AS events of other pre-mRNAs. In this study, we analyzed AS events of SR proteins in Arabidopsis thaliana ...
Transgenic and Gene Targeting Core
... responsibility for such mice and all risks of harm they may cause including, but not limited to, any injury resulting from the handling of the mice. ...
... responsibility for such mice and all risks of harm they may cause including, but not limited to, any injury resulting from the handling of the mice. ...
Role of histone and DNA methylation in gene regulation
... to be the main de novo DNA methylation activities in Arabidopsis, required for example for the establishment but not subsequent maintenance of silencing at FWA and SUPERMAN (SUP) loci [26]. DRM1/2 are also required for non-CG methylation at other chromosomal targets [27,28]. The discovery of the inv ...
... to be the main de novo DNA methylation activities in Arabidopsis, required for example for the establishment but not subsequent maintenance of silencing at FWA and SUPERMAN (SUP) loci [26]. DRM1/2 are also required for non-CG methylation at other chromosomal targets [27,28]. The discovery of the inv ...
Trachemys scripta elegans Red-Eared Turtle ( Fc)
... IgY is a low m.w. serum Ab encoded by the y gene in nonmammalian tetrapods, including birds (6–8), reptiles (9, 10), and amphibians (11–14). The gene is thought to have developed from m by a duplication event (2, 15) because of their sequence homology and similar gene structures. It is also thought ...
... IgY is a low m.w. serum Ab encoded by the y gene in nonmammalian tetrapods, including birds (6–8), reptiles (9, 10), and amphibians (11–14). The gene is thought to have developed from m by a duplication event (2, 15) because of their sequence homology and similar gene structures. It is also thought ...
Initiation of recombination suppression and PAR formation during
... sex chromosomes might diverge largely because of not only their ancient origin, but also an unrecognized mechanism that accelerates Y degeneration. It is needed to understand the both processes of recombination suppression and PAR formation during early sex chromosome differentiation for revealing t ...
... sex chromosomes might diverge largely because of not only their ancient origin, but also an unrecognized mechanism that accelerates Y degeneration. It is needed to understand the both processes of recombination suppression and PAR formation during early sex chromosome differentiation for revealing t ...
Xylitol production using recombinant Saccharomyces
... exposure to UV light. The 1 kb-BamHI fragment containing the neo r gene was isolated from plasmid pIDXR and labeled by the random priming method using a Genius kit (Boehringer Mannheim). The probe could hybridize with both pITyXR and pIDXR. Prehybridization, hybridization, and subsequent nonradioact ...
... exposure to UV light. The 1 kb-BamHI fragment containing the neo r gene was isolated from plasmid pIDXR and labeled by the random priming method using a Genius kit (Boehringer Mannheim). The probe could hybridize with both pITyXR and pIDXR. Prehybridization, hybridization, and subsequent nonradioact ...
final1-final-report-cig
... tumorigenesis. The specific aims we proposed are: 1) exploring the least conserved parts of BRCA2 protein 2) by studying the functional impact of BRCA2 variants of unknown clinical relevance identified in families at high risk of breast cancer. 3) In addition, we are developing new tools based on BR ...
... tumorigenesis. The specific aims we proposed are: 1) exploring the least conserved parts of BRCA2 protein 2) by studying the functional impact of BRCA2 variants of unknown clinical relevance identified in families at high risk of breast cancer. 3) In addition, we are developing new tools based on BR ...
Gene organization, transcription signals and processing of the
... detected between this ORF and those flanking other archaebacterial rRNA operons (unpublished work). Initiation and processing of the 16S RNA leader transcript It has been shown previously by S1 nuclease mapping that initiation of rRNA transcription occurs at a single site 175 bp upstream from the 16 ...
... detected between this ORF and those flanking other archaebacterial rRNA operons (unpublished work). Initiation and processing of the 16S RNA leader transcript It has been shown previously by S1 nuclease mapping that initiation of rRNA transcription occurs at a single site 175 bp upstream from the 16 ...
Eye on the Future: How can modern scientific knowledge help to
... diversity from a limited number of genes, and it is more important than has previously been recognised. The splicer zone is the protein complex that actually deals with doing this. I am going to give you a little primer of bacterial biochemistry because you know what is coming next, which is antibio ...
... diversity from a limited number of genes, and it is more important than has previously been recognised. The splicer zone is the protein complex that actually deals with doing this. I am going to give you a little primer of bacterial biochemistry because you know what is coming next, which is antibio ...
A Complete Mitochondrial Genome Sequence
... Summary of Illumina Genome Analyzer Sequencing and mtDNA Sequence Assembly A total of 49,125,583 Illumina Genome Analyzer (GA) single sequence reads, each 36 nucleotides in length, were produced from three independently prepared CPC98 Illumina GA libraries and sequenced across 14 lanes of three flow ...
... Summary of Illumina Genome Analyzer Sequencing and mtDNA Sequence Assembly A total of 49,125,583 Illumina Genome Analyzer (GA) single sequence reads, each 36 nucleotides in length, were produced from three independently prepared CPC98 Illumina GA libraries and sequenced across 14 lanes of three flow ...
miRNA pptx - NUS School of Computing
... use the expression level of a known target genes for that specific intergenic miRNA If its target genes are consistently down-regulated, then we can assume that the expression level of the intergenic miRNA gene is up-regulated ...
... use the expression level of a known target genes for that specific intergenic miRNA If its target genes are consistently down-regulated, then we can assume that the expression level of the intergenic miRNA gene is up-regulated ...