- LSHTM Research Online
... limited by the technical constraints of STM screens, which allow pools of only 102 to 103 mutants to be analyzed. While these studies proved useful for identifying a limited number of virulence factors and even potential live-vaccine candidates (11), they were able to assay only a small portion of t ...
... limited by the technical constraints of STM screens, which allow pools of only 102 to 103 mutants to be analyzed. While these studies proved useful for identifying a limited number of virulence factors and even potential live-vaccine candidates (11), they were able to assay only a small portion of t ...
Beyond traditional paternity and identification cases
... Based on the information above, one may generate all possible pedigrees containing only these individuals. However, one will frequently be interested in pedigrees involving persons not included in the original group. For example, to describe that a woman has three children with the same man, it is n ...
... Based on the information above, one may generate all possible pedigrees containing only these individuals. However, one will frequently be interested in pedigrees involving persons not included in the original group. For example, to describe that a woman has three children with the same man, it is n ...
Mating-Type Genes From the Homothallic Fungus Sordaria
... macrospora genomic DNA and cosmid DNAs revealed that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region ...
... macrospora genomic DNA and cosmid DNAs revealed that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region ...
Full-Text PDF
... In all eukaryotic organisms, genome replication depends on activity of the primosome, a four-subunit complex of DNA primase and DNA polymerase α (Polα) [1]. The primosome initiates synthesis of both the leading and lagging strands by making chimeric RNA-DNA primers, which are required for the loadin ...
... In all eukaryotic organisms, genome replication depends on activity of the primosome, a four-subunit complex of DNA primase and DNA polymerase α (Polα) [1]. The primosome initiates synthesis of both the leading and lagging strands by making chimeric RNA-DNA primers, which are required for the loadin ...
Part 4 Student Handout - URMC
... They look to see where your DNA pieces end up on the gel. If you have the HD gene, some of your gene copies won’t move as far in the gel as normal pieces do. They’ll be closer to the wells on the gel than the normal genes. ...
... They look to see where your DNA pieces end up on the gel. If you have the HD gene, some of your gene copies won’t move as far in the gel as normal pieces do. They’ll be closer to the wells on the gel than the normal genes. ...
Biomart/ GENOME ALIGNMENT III
... and the mouse sequence. Even in remote organisms such as fugu and zebrafish some of the human exons are conserved. Between rat and mouse and human in the region 1000 bp upstream of the first exons parts of the sequence are conserved as well. These might correspond to the regulatory motifs, responsib ...
... and the mouse sequence. Even in remote organisms such as fugu and zebrafish some of the human exons are conserved. Between rat and mouse and human in the region 1000 bp upstream of the first exons parts of the sequence are conserved as well. These might correspond to the regulatory motifs, responsib ...
Bioinformatics Dr. Víctor Treviño Pabellón Tec
... the contribution of tht alignment to the msa. For example, if an extra copy of one of the sequences is added to the alignment project, then for sequence pairs that do not include that sequence will increase, indicating a lesser role because the contributions of that pair have been out-voted by the a ...
... the contribution of tht alignment to the msa. For example, if an extra copy of one of the sequences is added to the alignment project, then for sequence pairs that do not include that sequence will increase, indicating a lesser role because the contributions of that pair have been out-voted by the a ...
Unit 8 - OpenWetWare
... the information needed to reconstruct the other half by the mechanism of base pairing. In most prokaryotes, DNA replication begins at a single point and continues in two directions. ...
... the information needed to reconstruct the other half by the mechanism of base pairing. In most prokaryotes, DNA replication begins at a single point and continues in two directions. ...
Cloning of genes from genomic DNA: Part 3
... little bit of each (PCR product and genomic) on an agarose gel to see what we have. Before we can begin the restriction enzyme digests, we need to “clean up” our PCR products. As they are now, the PCR reaction contains the PCR buffer (which is not correct for the enzymes) and lots of unused oligonuc ...
... little bit of each (PCR product and genomic) on an agarose gel to see what we have. Before we can begin the restriction enzyme digests, we need to “clean up” our PCR products. As they are now, the PCR reaction contains the PCR buffer (which is not correct for the enzymes) and lots of unused oligonuc ...
PDF
... Maternal effects have been genetically defined by mutations that affect the next generation when inherited from the mother. Maternal effect genes play an essential role in early development in many animal species (Riechmann and Ephrussi, 2001; Sardet et al., 2004). In plants, the maternal contributi ...
... Maternal effects have been genetically defined by mutations that affect the next generation when inherited from the mother. Maternal effect genes play an essential role in early development in many animal species (Riechmann and Ephrussi, 2001; Sardet et al., 2004). In plants, the maternal contributi ...
Inheritance of Red Green - Department Of Biological Sciences
... These data are consistent with a model in which the loci responsible for inherited variations in color vision correspond to the genes that encode the apoproteins of the three cone pigments. In support of this model, Rushton, as well as Alpem and Wake, have M OST HUMANS CAN MATCH ANY COLOR EITHER BY ...
... These data are consistent with a model in which the loci responsible for inherited variations in color vision correspond to the genes that encode the apoproteins of the three cone pigments. In support of this model, Rushton, as well as Alpem and Wake, have M OST HUMANS CAN MATCH ANY COLOR EITHER BY ...
An Introduction to Hidden Markov Models
... • Each state path has a different annotation for the location of the 5’ splice site (white boxes). • The likelihood of a splice site at a specific position of the sequence can be calculated by taking the probability of all state paths that assign the splice site to that position and dividing it by t ...
... • Each state path has a different annotation for the location of the 5’ splice site (white boxes). • The likelihood of a splice site at a specific position of the sequence can be calculated by taking the probability of all state paths that assign the splice site to that position and dividing it by t ...
Protein splicing elements: inteins and exteins
... All of the known inteins have sequence similarity to homing endonucleases (reviewed in 14,15,17-20,22-23), but not all of them have been tested for endonuclease activity. Once endonuclease activity has been established, intein-derived endonucleases should be named according to the standard homing en ...
... All of the known inteins have sequence similarity to homing endonucleases (reviewed in 14,15,17-20,22-23), but not all of them have been tested for endonuclease activity. Once endonuclease activity has been established, intein-derived endonucleases should be named according to the standard homing en ...
Journal of Bacteriology
... metals to the free thiols of periplasmic proteins which are normally oxidized by the dsbA gene product. Jungmann et al. (8) have shown that in yeast, mutants deficient in specific ubiquitin-conjugating enzymes are hypersensitive to Cd21. Moreover, mutants in the proteasome were also hypersensitive t ...
... metals to the free thiols of periplasmic proteins which are normally oxidized by the dsbA gene product. Jungmann et al. (8) have shown that in yeast, mutants deficient in specific ubiquitin-conjugating enzymes are hypersensitive to Cd21. Moreover, mutants in the proteasome were also hypersensitive t ...
Article A Distinct Mitochondrial Genome with DUI
... eukaryotes, and each mitochondrion possesses multiple copies of species-specific mitochondrial genome. Mitochondrial DNA (mtDNA), albeit circular, doublestranded and short (&16.5 kb), is of crucial importance because genetic variation within the encoded sequence of mitochondrial protein subunits can ...
... eukaryotes, and each mitochondrion possesses multiple copies of species-specific mitochondrial genome. Mitochondrial DNA (mtDNA), albeit circular, doublestranded and short (&16.5 kb), is of crucial importance because genetic variation within the encoded sequence of mitochondrial protein subunits can ...
Use of QTL analysis in physiological research
... genes are located within the two-LOD intervals of various QTLs, making them likely candidates for the observed variation. However, for various Susy QTLs, no Susy gene has been described in the 2-LOD interval, and hence the QTL is likely to be caused by another gene, the identity of which has to be r ...
... genes are located within the two-LOD intervals of various QTLs, making them likely candidates for the observed variation. However, for various Susy QTLs, no Susy gene has been described in the 2-LOD interval, and hence the QTL is likely to be caused by another gene, the identity of which has to be r ...
Bruce Wallace Biotechnology Lab Program Student Guide 5th
... molecular biology have been modified through genetic engineering to facilitate gene cloning and protein production (gene expression) in bacteria. Antibiotic resistant genes have been engineered into these plasmids and function as selectable markers—that is to say, these genes allow us to select betw ...
... molecular biology have been modified through genetic engineering to facilitate gene cloning and protein production (gene expression) in bacteria. Antibiotic resistant genes have been engineered into these plasmids and function as selectable markers—that is to say, these genes allow us to select betw ...
Identify differential APA usage from RNA
... one wants to analyze - these could be given using a gtf file or a GRanges object. The gtf file should have an attribute (metadata column for the GRanges object) called “gene id” that ends with “ PRE” or “ POST” to address respectively the short and the long isoforms. An element in the annotation is ...
... one wants to analyze - these could be given using a gtf file or a GRanges object. The gtf file should have an attribute (metadata column for the GRanges object) called “gene id” that ends with “ PRE” or “ POST” to address respectively the short and the long isoforms. An element in the annotation is ...
Polymorphism of FecB Gene in Nine Sheep Breeds or Strains and
... probably resulted from the method of breeding. Traditionally, sheep were maintained in family flocks of about three or four ewes, many families relying on neighbors for a ram in a village. The aim of breeding was high prolificacy and high quality of lambskins. Ewes and rams were selected on the basi ...
... probably resulted from the method of breeding. Traditionally, sheep were maintained in family flocks of about three or four ewes, many families relying on neighbors for a ram in a village. The aim of breeding was high prolificacy and high quality of lambskins. Ewes and rams were selected on the basi ...
Supporting Information Legends
... in RAP-DB. The gray arrow represents LOC_Os04g59620, which is a paralog of LOC_Os04g59624. (b) Comparison of the nucleotide sequences of the third exon of LOC_Os04g59624 between WT (upper letters) and enl1 (lower letters). The alignment revealed the presence of a T->A substitution and a nearby smal ...
... in RAP-DB. The gray arrow represents LOC_Os04g59620, which is a paralog of LOC_Os04g59624. (b) Comparison of the nucleotide sequences of the third exon of LOC_Os04g59624 between WT (upper letters) and enl1 (lower letters). The alignment revealed the presence of a T->A substitution and a nearby smal ...
The nucleotide sequence of Saccharomyces cerevisiae
... IX contains 221 open reading frames (ORFs), of which approximately 30% have been sequenced previously. This chromosome shows features typical of a small Saccharomyces cerevisiae chromosome. The sequence derived for chromosome IX is 439,886 nucleotides in length, and 71.6% codes for proteins or predi ...
... IX contains 221 open reading frames (ORFs), of which approximately 30% have been sequenced previously. This chromosome shows features typical of a small Saccharomyces cerevisiae chromosome. The sequence derived for chromosome IX is 439,886 nucleotides in length, and 71.6% codes for proteins or predi ...
Extranuclear protection of chromosomal DNA from
... and Contributes to the Stress-Resistance Phenotype. A genome-wide ...
... and Contributes to the Stress-Resistance Phenotype. A genome-wide ...
Microcin B17 Blocks DNA Replication and Induces
... the colicins, is non-lethal for the producing cell, and is not stimulated by agents which induce the SOS response (Baquero & Moreno, 1984). In most cases microcin production is plasmiddependent and, hitherto, five types of microcins have been identified by cross-immunity, biochemical and genetic cri ...
... the colicins, is non-lethal for the producing cell, and is not stimulated by agents which induce the SOS response (Baquero & Moreno, 1984). In most cases microcin production is plasmiddependent and, hitherto, five types of microcins have been identified by cross-immunity, biochemical and genetic cri ...
An extreme cytoplasmic bottleneck in the modern European
... 1993) have con¢rmed these low levels of cytoplasmic diversity, although RFLP analysis of chloroplast DNA has historically revealed little intraspeci¢c diversity due to extremely low levels of nucleotide substitution in the chloroplast genome (Wolfe et al. 1987; Soltis et al. 1992). Low levels of fer ...
... 1993) have con¢rmed these low levels of cytoplasmic diversity, although RFLP analysis of chloroplast DNA has historically revealed little intraspeci¢c diversity due to extremely low levels of nucleotide substitution in the chloroplast genome (Wolfe et al. 1987; Soltis et al. 1992). Low levels of fer ...
