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1_genomics
1_genomics

... and Frederick Sanger at the U.K. ...
Slajd 1
Slajd 1

... SDS-PAGE • SDS is an ionic detergent that readily binds to protein and gives it an overall negative charge. This allows the protein to travel towards the (+) charge, and the fragments separate according to its molecular weight as it travels. ...
DNA Computer Review
DNA Computer Review

... a. Go to http://www.courses.fas.harvard.edu/~biotext/animations/TRANSLATE20b.swf b. What do tRNA’s do? c. What part of the tRNA binds to the codons of the mRNA? d. Go to http://learn.genetics.utah.edu/content/begin/dna/transcribe/ e. Go to http://www.biostudio.com/demo_freeman_protein_synthesis.htm ...
DNA and RNA
DNA and RNA

... • Hydrogen bonds hold base pairs together • Nobel Prize 1958 ...
recombinant dna lab
recombinant dna lab

... DNA fragments from donor cells must become part of the genetic material of living cells before the genes they contain can be activated. For example, DNA fragments may be combined with bacterial DNA so that they can later be inserted into a bacterial cell. Bacteria often contain small circular DNA mo ...
File
File

File - Mr. Polls Science
File - Mr. Polls Science

... protein which in turn codes for a trait. Hence you hear it commonly referred to as the gene for baldness or the gene for blue eyes. Meanwhile, DNA is the chemical that genes and chromosomes are made of. DNA is called a nucleic acid because it was first found in the nucleus. We now know that DNA is a ...
ASviewer: Visualizing the transcript structure and functional
ASviewer: Visualizing the transcript structure and functional

... Summary: Alternative splicing (AS) produces diverse transcript structures by differential use of splice sites. Comparing the gene structure and functional domains of splice variants is an essential but nontrivial task with numerous gene predictions available publicly. We developed a novel viewer (AS ...
Workshop #4 - Chemistry part2
Workshop #4 - Chemistry part2

... 1. What are the building block unit of proteins? How do these building blocks differ from each other? 2. List three structural differences and one functional difference between DNA and RNA. PROVIDE THE APPROPRIATE TERM TO COMPLETE EACH STATEMENT. 1. The most abundant protein in your body is collagen ...
Molecular Genetics Notes
Molecular Genetics Notes

... the genetic message to the ribosome where proteins are produced ...
Lab22
Lab22

... 95°C, 30sec = denaturation: separate the double stranded DNA template (break H-bonds) 50-60°C, 30sec = annealing: allows primers to complementary base pair with target sequence on template DNA, temp varied depending on %GC content 72°C, 1min/kb DNA = extension: optimal temp for DNA polymerase to syn ...
Annotation of Drosophila virilis
Annotation of Drosophila virilis

... frame with coding region of similarity (+3)  For each putative intron/exon boundary compare location of BLASTX result to locate exact first and last base of the exon such that the conserved amino acids are linked together in a single long open reading frame ...
Human Genome Project
Human Genome Project

... Energy and the National Institute of Health (NIH) in 1989. NIH provided three million dollars to initiate the project; mostly it was a government sponsored research. In 1998 Craig Venter a former researcher of NIH during early 1990s and his firm, Celera Genomics launched a similar type of project wh ...
Gregor Mendel Mendel`s 7 Pea Plant Traits
Gregor Mendel Mendel`s 7 Pea Plant Traits

... Contains Genes GENE - region of DNA that produces a functional protein Eg. The Purple Pigment Protein which give a flower its purple color are “encoded” by a specific set of genes. ...
Collect, analyze and synthesize
Collect, analyze and synthesize

... frame with coding region of similarity (+3) l For each putative intron/exon boundary compare location of BLASTX result to locate exact first and last base of the exon such that the conserved amino acids are linked together in a single long open reading frame l ...
Document
Document

... A. Genes and Protein 1.Gene - a specific sequence of bases in DNA that determines the sequence of amino acids in a protein ...
GENETIC ENGINEERING (ppt)
GENETIC ENGINEERING (ppt)

... a Chinook salmon that allows the fish to produce growth hormone yearround. Scientists were able to keep the hormone active by using a gene from an eel-like fish called an ocean pout, which acts as an “on switch” for the hormone. If the FDA approves the sale of the salmon, it will be the first time t ...
Making Sentences of DNA
Making Sentences of DNA

... the sequence of the DNA where it says DNA strand in the table. 2. With the DNA sequences you must now transcribe the DNA into mRNA (the message). 3. You will now translate the message by translating the mRNA to find the tRNA anti-codons you will look for in the cytoplasm ...
Central Dogma: Molecular GeneKcs
Central Dogma: Molecular GeneKcs

... Genome Projects of some eukaryotic organisms ...
Lecture #12 - Suraj @ LUMS
Lecture #12 - Suraj @ LUMS

... Lecture 12 DNA Replication Molecular Biology of the Gene ...
Cloning and Characterization of the KlDIM1 Gene
Cloning and Characterization of the KlDIM1 Gene

... 320-amino acid protein which shows 81% identity to ScDim1p from S. cerevisiae and 25% identity to ksgAp from Escherichia coli. Complementation of the kasugamycin-resistant ksgA-mutant of E. coli lacking dimethylase activity demonstrates that KlDim1p is the functional homologue of the bacterial enzym ...
GMO and Biotechnology
GMO and Biotechnology

... Foreign DNA is common (via nature) in most genomes, Transgenes must be expressed in order to function, Promoters control where, when and how much protein is produced. ...
Lect11_DNAMethylation
Lect11_DNAMethylation

Nucleic Acids
Nucleic Acids

... • Adenine (A) and thymine (T) form strong hydrogen bonds to each other but not to C or G • Guanine (G) and cytosine (C) form strong hydrogen bonds to each other but not to A or T ...
Polymers
Polymers

... History of DNA  Scientists were aware that DNA had a sugar phosphate background and had bases A, T, G and C but were not sure about the actual shape of DNA.  Wilkins and Franklin used X-ray crystallography to create images of DNA’s structure.  Watson discovered that the base pairs A to T were ex ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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