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Defining the Epigenetic Mechanism of Asymmetric Cell Division of
Defining the Epigenetic Mechanism of Asymmetric Cell Division of

... chromatids such that one specific member of the two is imprinted. The cell inheriting the template (arbitrarily named) Watson strand-containing chromatid is switching competent, and its sister cell, inheriting the template Crick strand-containing chromatid, is always incompetent (Klar 1987a, 1990, 20 ...
Inactivation of Antibiotics and the Dissemination of
Inactivation of Antibiotics and the Dissemination of

... ~-[actam substrate. Other resistance mechanisms evolve in response to continued 13-1actam use. In some cases, increased resistance resuhs from increased expression of the [3-1actamase through an up-promoter mutation (10), or chromosomal 13-1actamase genes can be overexpressed in highly resistant str ...
AnsteadSeniorHonorsThesis
AnsteadSeniorHonorsThesis

... chromosome, which span multiple genes. To measure the results, two genetic markers will be attached to the mutants with deficiencies: curly wings (compared to the wild-type straight wings), located on the second chromosome, and stubble-hair (compared to the bristles found in wild-type), located on t ...
Supplementary Data The complete 12 Mb genome and
Supplementary Data The complete 12 Mb genome and

... reported by contrasting rpoB(R)N426H strain to wild type strain data. Gene-sets with Normalized Enrichment Score (NES) > 1.70 and False Discovery Rate (FDR) < 0.1 in at least one of the contrasts are reported. Green and red colors indicate, respectively, up-regulation and down-regulation in test str ...
The msh2 Gene of Schizosaccharomyces pombe Is
The msh2 Gene of Schizosaccharomyces pombe Is

... DNA-binding domain. Cytological analysis revealed that during meiotic prophase of msh2-defective cells, chromosomal structures were frequently formed; such structures are rarely found in the wild type. Our data show that besides having a function in mismatch repair, S. pombe msh2 is required for cor ...
20Sexual Reproduction, Meiosis, and Genetic Recombination
20Sexual Reproduction, Meiosis, and Genetic Recombination

... base sequence. Mutations are rare events, and beneficial mutations are even rarer. But when beneficial mutations do arise, it is clearly advantageous to preserve them in the population. It can be even more beneficial to bring mutations together in various combinations—and therein lies an advantage o ...
Cover Letter
Cover Letter

... One thing is interesting is that genes 1 to 37 are forward, the rest of the genes are all reverse. Selected notes from PECAAN which cover debatable and difficult calls. CDS 4408 - 4668 /note=According to Phage DB blast, similarity shown to structural protein; according to HHPRED, similarity to tail ...
ARTICLES - Weizmann Institute of Science
ARTICLES - Weizmann Institute of Science

... for histone–DNA association3); second, when constructing the model we represent the two-fold symmetry axis of the nucleosome structure1 by including the reverse complement of each sequence in the nucleosome collection. More sophisticated nucleosome–DNA interaction models based on mixture models15 or ...
Structural organization of the transfer RNA gene clusters of cholera
Structural organization of the transfer RNA gene clusters of cholera

... phage φ 149 genome. The results presented here show that the tRNA genes are contained in two HindIII fragments, 11 and 3·4 kb in length. These two fragments do not bear any sequence homology and the smallest fragment alone carried the genes for tRNAs specific for nine different amino acids. Secondly ...
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA

... mitochondrial genome is 16,569 base pairs (Fig. 1). Despite its small size, mtDNA encodes 22 transfer RNAs, 2 ribosomal RNAs and 13 polypeptides which are required for OXPHOS. Although the number 1000 is widely quoted as the mtDNA complement of a typical cell, there is in fact considerable variation ...
Introduction to Bioinformatics.
Introduction to Bioinformatics.

Structural and Functional Studies of Insertion Element IS200
Structural and Functional Studies of Insertion Element IS200

... right end of hisD98-l: : ISZOO. Since the deletions were selected for hid gene expression. all must retain the hi& gene. Two exceptions are deletions his-9543 and 9545 (see Fig. 4). which express hid’ despite removal of a small. apparent,ly dispensable. part of the hid? gene. To examine whether the ...
The Theory of Electrophoresis
The Theory of Electrophoresis

... biomolecules (protein & nucleic acids) using an electric field. The factors which govern the movement of the biomolecules through the electric field include: • charge, • size, • shape of the biomolecules ...
Gene targeting: vector design and construction
Gene targeting: vector design and construction

... your gene of interest? – Degree of homology may depend on the gene. – look at the genes surrounding your gene. If you find synteny, it is OK. • Which chromosome? Three copies of chromosome #2; single copy of chromosome Z in DT40, which was derived from female chicken. • Analyze/map genomic structure ...
Bio 100 Placement Study Guidelines
Bio 100 Placement Study Guidelines

... 2.! Explain how hydrogen bonds contribute to the structure of DNA. 3.! Use a template strand of nucleotides to create a complementary strand. 4.! Explain the roles of DNA, RNA, and protein in gene function. 5.! Describe the events in transcription. 6.! Explain how RNA is processed during transcripti ...
Plants` Epigenetic Secrets
Plants` Epigenetic Secrets

... There are three different types of DNA methylation in plants: CG, CHH (where H is any base except G), and CHG. In Arabidopsis, CG methylation is found on some genes, but primarily on repeat sequences that make up transposons, as well as other repeat sequences in the genome. CHH methylation is found ...
Indications, Contraindications, and Limitations
Indications, Contraindications, and Limitations

... depth of 644X) to not impact assay performance in these regions. The BLAST analysis for the 271 BRCA2 oligonucleotide baits against the human genomic database did not identify any sequences with significant homology. ...
Teacher Guide - Science Take-Out
Teacher Guide - Science Take-Out

... Before the 1980’s, growth hormone for injections was isolated from human pituitary glands ...
Tasting Phenylthiocarbamide (PTC): A New Integrative
Tasting Phenylthiocarbamide (PTC): A New Integrative

... using Fnu4H1. Following electrophoretic separation of the digestion products, nontaster homozygotes will still see only the 303 base pair fragment on the gel, while taster homozygotes will see two shorter fragments (239bp and 65bp) and taster heterozygotes will see all three fragment lengths. A typi ...
Part III: Laboratory – Electrophoresis
Part III: Laboratory – Electrophoresis

... approximately an eighth of an inch in diameter. If the leaves are too small, take tissue from multiple leaves (from the same plant) until you have the equivalent amount of leaf tissue. Note: Plants with the ago-1 phenotype are so small that you may have to use the entire plant. If you use the entire ...
Conceptual Integrated Science—Chapter 16
Conceptual Integrated Science—Chapter 16

... a new molecule of double-stranded DNA is created that is identical to the original. one old strand is used to assemble one new strand. the A nucleotide matches with the T and the C with the G. all of the above occur. ...
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2

... of DNA damage, double-strand DNA breaks (DSBs) are caused by ionizing radiation, genotoxic chemicals or errors in DNA replication (Kuzminov, 2001; Tonami et al., 2005). Failure to correctly repair DSBs can cause genome instability, mutations, cell cycle arrest and even cell death (Glazer & Glazunov, ...
Epigenetics and the environment: emerging
Epigenetics and the environment: emerging

... Box 1 | Altered epigenetic states and phenotypes in plants In plants, there are many natural examples of stable alterations in DNA methylation that confer specific phenotypes. These include the Colourless non-ripening (Cnr) locus in tomato, at which heritable hypermethylation induces repression and ...
Chapter 18: Gene Mutation and DNA Repair
Chapter 18: Gene Mutation and DNA Repair

... a. A change in a single base pair in the DNA. b. A mutation that does not change the amino acid sequence of the polypeptide. c. The addition or deletion of one or two nucleotides. d. A change that alters a single amino acid in the polypeptide. e. A physical change in the structure of the chromosome. ...
genes is conserved among species related to
genes is conserved among species related to

... 1996). Variation in expression of surface-exposed proteins is known as antigenic variation and is often found for (a)fimbrial adhesins of pathogenic or opportunistic bacteria that are able to adhere to the epithelial cell layer of the gastro-intestinal tract of the host. In this study we have determ ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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