Inglés
... Access to sequence data is critical, and much of the new sequence data are distributed over the Internet. The Internet also provides a means to distribute software, and enables researchers to perform sophisticated analyses on remote servers. It was fortuitous, but fortunate, that the Human Genome Pr ...
... Access to sequence data is critical, and much of the new sequence data are distributed over the Internet. The Internet also provides a means to distribute software, and enables researchers to perform sophisticated analyses on remote servers. It was fortuitous, but fortunate, that the Human Genome Pr ...
P-Element Transformation with period Locus DNA Restores
... transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We do not have enough molecular information to interpret the apparently subnormal activity of the two rescuing DNA s ...
... transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We do not have enough molecular information to interpret the apparently subnormal activity of the two rescuing DNA s ...
OsHUS1 Facilitates Accurate Meiotic Recombination in Rice
... Natural Science Foundation of China (31230038 and 31170288). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: [email protected] ...
... Natural Science Foundation of China (31230038 and 31170288). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: [email protected] ...
voltammetric methods for determination of some anticancer drugs
... DNA is a structurally polymorphic macromolecule which, depending on the nucleotide sequence and environmental conditions, can adopt a variety of conformations. The double helical structre of DNA consists of two strands, each of them on the outside of the double helice and formed by altemating phosph ...
... DNA is a structurally polymorphic macromolecule which, depending on the nucleotide sequence and environmental conditions, can adopt a variety of conformations. The double helical structre of DNA consists of two strands, each of them on the outside of the double helice and formed by altemating phosph ...
Allele Mining Strategies: Principles and Utilisation for Blast
... through gene pyramiding. The actual identification of candidate DNA markers linked to resistance genes using fine mapping may well allow rice breeders to efficiently transfer these genes from donor cultivars into new, elite rice cultivars using marker-assisted selection. There is also a need to conn ...
... through gene pyramiding. The actual identification of candidate DNA markers linked to resistance genes using fine mapping may well allow rice breeders to efficiently transfer these genes from donor cultivars into new, elite rice cultivars using marker-assisted selection. There is also a need to conn ...
Carbapenemase and virulence factors of
... assay. When these experiments failed, plasmid DNA was extracted with NucleoBondw Xtra (Macherey-Nagel, Germany) and transferred by electroporation into a bacterial recipient. Selection was performed on agar plates supplemented with ticarcillin (32 mg/L) and rifampicin (300 mg/L) for the mating-out a ...
... assay. When these experiments failed, plasmid DNA was extracted with NucleoBondw Xtra (Macherey-Nagel, Germany) and transferred by electroporation into a bacterial recipient. Selection was performed on agar plates supplemented with ticarcillin (32 mg/L) and rifampicin (300 mg/L) for the mating-out a ...
Divergent Evolution of Duplicate Genes Leads to Genetic
... interaction between the LD1.5 alleles and HPA genotypes argues that HPA is involved in LD1 epistasis. Crosses to the emb2196 mutant led to the same conclusion when Col and Cvi alleles were compared versus the stronger emb2196 allele (Fig. 3B). However, a heterozygous seedling for emb2196 mutation (C ...
... interaction between the LD1.5 alleles and HPA genotypes argues that HPA is involved in LD1 epistasis. Crosses to the emb2196 mutant led to the same conclusion when Col and Cvi alleles were compared versus the stronger emb2196 allele (Fig. 3B). However, a heterozygous seedling for emb2196 mutation (C ...
Highly precise and developmentally programmed genome
... thousands of short, non-coding germline sequences (Internal Eliminated Sequences or IESs), each one flanked by two TA dinucleotides. It has been reported previously that these genome rearrangements are initiated by the introduction of developmentally programmed DNA double-strand breaks (DSBs), which ...
... thousands of short, non-coding germline sequences (Internal Eliminated Sequences or IESs), each one flanked by two TA dinucleotides. It has been reported previously that these genome rearrangements are initiated by the introduction of developmentally programmed DNA double-strand breaks (DSBs), which ...
Joint analysis of the influence of CYP11B1 and DGAT1 genetic
... DGAT1K) of son j within sire i, b1 and b2 are the regression coefficients representing half of the gene substitution effect (α/2), and eij is the random residual effect including polygenic and environmental effects. Sires were included as fixed effects because they represent highly selected animals ...
... DGAT1K) of son j within sire i, b1 and b2 are the regression coefficients representing half of the gene substitution effect (α/2), and eij is the random residual effect including polygenic and environmental effects. Sires were included as fixed effects because they represent highly selected animals ...
Biotechnology Explorer™ Ligation and Transformation - Bio-Rad
... base pairs (bp) long, although most plasmids used in cloning are 2,000–10,000 bp. Bacteria may naturally contain many copies of a single plasmid, or single copies of others. Plasmids are able to replicate independently of the host DNA and most plasmids carry at least one gene. Frequently these genes ...
... base pairs (bp) long, although most plasmids used in cloning are 2,000–10,000 bp. Bacteria may naturally contain many copies of a single plasmid, or single copies of others. Plasmids are able to replicate independently of the host DNA and most plasmids carry at least one gene. Frequently these genes ...
Principles and challenges of genome
... signal intensities can be used to extract DNA methylation information at the corresponding loci on the array. DMH is one of several techniques that incorporate precutting of genomic DNA with the restriction enzyme MseI. The recognition site of MseI is AATT, so it cuts frequently in the genome but re ...
... signal intensities can be used to extract DNA methylation information at the corresponding loci on the array. DMH is one of several techniques that incorporate precutting of genomic DNA with the restriction enzyme MseI. The recognition site of MseI is AATT, so it cuts frequently in the genome but re ...
Ribosome Profiling
... technique has been developed, known as Ribosome profiling (RP). RP is defined as a genome wide mapping of nucleotides on mRNA occupied by ribosomes during translation at a specific time. Generally polysome profiling is used to distinguish mRNA molecules involved in active translation from the non-tr ...
... technique has been developed, known as Ribosome profiling (RP). RP is defined as a genome wide mapping of nucleotides on mRNA occupied by ribosomes during translation at a specific time. Generally polysome profiling is used to distinguish mRNA molecules involved in active translation from the non-tr ...
Functional genomics of plant photosynthesis in
... tetratricopeptide-like repeats (TPRs) in its C-terminal domain. TPR motifs might coordinate the assembly of proteins into multisubunit complexes, and indeed the Nac2 protein was shown to be part of a high molecular weight complex associated with non-polysomal RNA. RNA binding by Nac2 has not been sh ...
... tetratricopeptide-like repeats (TPRs) in its C-terminal domain. TPR motifs might coordinate the assembly of proteins into multisubunit complexes, and indeed the Nac2 protein was shown to be part of a high molecular weight complex associated with non-polysomal RNA. RNA binding by Nac2 has not been sh ...
Lampbrush Chromosomes of the Chicken
... from adult laying hens have been used in these studies. Fortuitously, LBC loops appear to be at their maximum extension in oocytes of this size range. By the time oocytes reach 'x,3-mm diam, the LBC stage begins to decline and both chromosomes and loops begin to contract. In making chicken LBC prepa ...
... from adult laying hens have been used in these studies. Fortuitously, LBC loops appear to be at their maximum extension in oocytes of this size range. By the time oocytes reach 'x,3-mm diam, the LBC stage begins to decline and both chromosomes and loops begin to contract. In making chicken LBC prepa ...
Genetic data indicate that proteins containing the GGDEF domain
... pYhcK £occulated heavily (Fig. 3B,C). Strong £uorescence was observed in and around these aggregates in the microscope after staining with Calco£uor (data not shown). Plasmid pYhcK caused the strongest aggregation in the liquid culture (Fig. 3C). These results support the data presented by Amikam an ...
... pYhcK £occulated heavily (Fig. 3B,C). Strong £uorescence was observed in and around these aggregates in the microscope after staining with Calco£uor (data not shown). Plasmid pYhcK caused the strongest aggregation in the liquid culture (Fig. 3C). These results support the data presented by Amikam an ...
Martina Šeruga Musić, Mladen Krajačić, Dijana Škorić
... For direct amplification of phytoplasma gene or pseudogene regions, three different primer pairs were used: 16R738f/16R1232r (GIBB et al., 1995), to amplify a part of 16S rRNA gene; fTufu/rTufu (SCHNEIDER et al., 1997), to amplify a part of tuf gene; and G35p/m (DAVIS et al., 1992), to amplify dnaB ...
... For direct amplification of phytoplasma gene or pseudogene regions, three different primer pairs were used: 16R738f/16R1232r (GIBB et al., 1995), to amplify a part of 16S rRNA gene; fTufu/rTufu (SCHNEIDER et al., 1997), to amplify a part of tuf gene; and G35p/m (DAVIS et al., 1992), to amplify dnaB ...
GROW`N`GLOW: THE ACE1 TWO-HYBRID
... MoBiTec's ACE1-based Grow'n'Glow yeast two-hybrid system is a version of a system originally developed by Fields and Song1. The yeast two-hybrid system has proven to be a powerful tool for identifying proteins from an expression library which can interact with one's protein of interest. The technolo ...
... MoBiTec's ACE1-based Grow'n'Glow yeast two-hybrid system is a version of a system originally developed by Fields and Song1. The yeast two-hybrid system has proven to be a powerful tool for identifying proteins from an expression library which can interact with one's protein of interest. The technolo ...
Gene Regulatory Network of Ikaros in T cell development and
... Ikaros is a master regulator of lymphogenesis, especially critical for lymphoid differentiation and maturation. Recurrent genomic lesions of Ikaros have been found associated with high risks of relapse of leukemia and poor outcome of therapy. However, it remains unclear about the gene regulatory net ...
... Ikaros is a master regulator of lymphogenesis, especially critical for lymphoid differentiation and maturation. Recurrent genomic lesions of Ikaros have been found associated with high risks of relapse of leukemia and poor outcome of therapy. However, it remains unclear about the gene regulatory net ...
Discovering the Distribution of Palindromic Sequences in the
... mutations on the SMAD4 database were converted into a readable .csv file (6 columns: (1) Segment (exon, etc.) (2) Type of mutation (3) Start location of the mutation (integer) (4) End location of the mutation (integer) (5) Nucleotide change (6) Type of protein change) titled “mutations.csv”. Three J ...
... mutations on the SMAD4 database were converted into a readable .csv file (6 columns: (1) Segment (exon, etc.) (2) Type of mutation (3) Start location of the mutation (integer) (4) End location of the mutation (integer) (5) Nucleotide change (6) Type of protein change) titled “mutations.csv”. Three J ...
Assessment by Molecular Dynamics Simulations of the Structural
... Sp1 consists of three consecutive zinc fingers that recognize a number of nucleotide sequences different from, but related to and sometimes overlapping, those recognized by the structurally better characterized early growth response protein 1 (EGR1, also known as Zif268, Krox-24, and NGFI-A). The ac ...
... Sp1 consists of three consecutive zinc fingers that recognize a number of nucleotide sequences different from, but related to and sometimes overlapping, those recognized by the structurally better characterized early growth response protein 1 (EGR1, also known as Zif268, Krox-24, and NGFI-A). The ac ...
First report of a tetracycline-inducible gene
... Mollicutes are a class of wall-less bacteria having arisen from ancestors common to low-G+C Gram-positive bacteria by a so-called regressive evolution (Weisburg et al., 1989). Various species are pathogenic to man, animals and plants, in which they may cause severe diseases. Due to their small genom ...
... Mollicutes are a class of wall-less bacteria having arisen from ancestors common to low-G+C Gram-positive bacteria by a so-called regressive evolution (Weisburg et al., 1989). Various species are pathogenic to man, animals and plants, in which they may cause severe diseases. Due to their small genom ...
- Wiley Online Library
... an optimum. An optimal strategy is one that (i) belongs to the strategy set and (ii) when given as input to the objective function, returns an output that is equal to or greater than that of all other strategies in the set. Formally, an optimal strategy is s* where U(s*) ‡ U(s) " s 2 S. Conversely, ...
... an optimum. An optimal strategy is one that (i) belongs to the strategy set and (ii) when given as input to the objective function, returns an output that is equal to or greater than that of all other strategies in the set. Formally, an optimal strategy is s* where U(s*) ‡ U(s) " s 2 S. Conversely, ...
MS26/CYP704B is required for anther and pollen wall
... To compare the wheat genes homologous to Ms26/CYP704B, genomic sequences corresponding to the three homeologs were obtained through BLAST (Basic Local Alignment Research Tool; [25]) of the TGACv1 wheat genome assembly (T. aestivum cv. Chinese Spring) (http:// plants.ensembl.org/Triticum_aestivum/Inf ...
... To compare the wheat genes homologous to Ms26/CYP704B, genomic sequences corresponding to the three homeologs were obtained through BLAST (Basic Local Alignment Research Tool; [25]) of the TGACv1 wheat genome assembly (T. aestivum cv. Chinese Spring) (http:// plants.ensembl.org/Triticum_aestivum/Inf ...
A formal theory of the selfish gene
... an optimum. An optimal strategy is one that (i) belongs to the strategy set and (ii) when given as input to the objective function, returns an output that is equal to or greater than that of all other strategies in the set. Formally, an optimal strategy is s* where U(s*) ‡ U(s) " s 2 S. Conversely, ...
... an optimum. An optimal strategy is one that (i) belongs to the strategy set and (ii) when given as input to the objective function, returns an output that is equal to or greater than that of all other strategies in the set. Formally, an optimal strategy is s* where U(s*) ‡ U(s) " s 2 S. Conversely, ...
The Novel Gene HOMOLOGOUS PAIRING
... little effect on bouquet formation (Pawlowski et al., 2004). The AHP2 of Arabidopsis (Arabidopsis thaliana) is homologous to Saccharomyces cerevisiae HOP2 (Leu et al., 1998) and S. pombe MEU13 (Nabeshima et al., 2001), which are known to act in monitoring homology between pairing partner at meiosis, ...
... little effect on bouquet formation (Pawlowski et al., 2004). The AHP2 of Arabidopsis (Arabidopsis thaliana) is homologous to Saccharomyces cerevisiae HOP2 (Leu et al., 1998) and S. pombe MEU13 (Nabeshima et al., 2001), which are known to act in monitoring homology between pairing partner at meiosis, ...