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Biology Performance Level Descriptors
Biology Performance Level Descriptors

... Standards for Biology. A student at this level has a sophisticated ability to describe genetic patterns of inheritance and how traits are defined by instructions encoded in many discrete genes, explain that Earth’s present-day species descended from common ancestral species due to variation and the ...
Interpretation of Complex Forensic DNA Mixtures
Interpretation of Complex Forensic DNA Mixtures

... come from the suspect. The suspect’s profile, if present, would be masked by the major profile at two additional loci. Only the victim’s alleles were evident at the remaining 7 loci. Although there are several strategies for interpreting such data, a conservative stance is suggested. Calculation of ...
Water - The Burge
Water - The Burge

... • DNA has three major functions! 1. DNA controls cellular activities including reproduction – DNA carries a code. Genetic instructions are encoded in the sequence of bases strung together in DNA. – DNA from male and DNA from female together become the genetic information of offspring in sexual repr ...
A Novel Method to Detect Identities in tRNA Genes Using Sequence
A Novel Method to Detect Identities in tRNA Genes Using Sequence

... We applied the method to Class I tRNAs to detect characteristic sites. We found that about 40% of characteristic sites that we detected are identities that have been detected experimentally, and that the remaining characteristic sites are in T and D domains which are the elbow regions of tRNAs. This ...
Dennis Vaughn1,John Jackson1, Matt Moscou24,Karin Werner24
Dennis Vaughn1,John Jackson1, Matt Moscou24,Karin Werner24

... 2003). Bioinformatic analysis was conducted to determine the genes, based on expression patterns, most likely to have been knocked out in m9467 and m9468. Primers were designed for those 48 genes and PCR (polymerase chain reaction) was used to identify the deletions. ...
() - Summer Programs
() - Summer Programs

... S/U grades will be based on the following assessments: ASSESSMENT TYPE Assignments: Seven homework problem sets Lab report detailing the construction of the recombinant plasmid and the expression and purification of the fusion protein. Exams: none Lab Notebook: a complete, accurate and up to date re ...
CLARK LAP Wednesday March 26 2014 STRAWBERRY DNA
CLARK LAP Wednesday March 26 2014 STRAWBERRY DNA

... through the cheesecloth and into the tall glass until there is very little liquid left in the funnel (only wet pulp remains). How does the filtered strawberry liquid look? • Pour the filtered strawberry liquid from the tall glass into the small glass jar so that the jar is one quarter full. • Measur ...
group_presentation
group_presentation

... difference, either a protein is not made at all, made in inadequate amounts, or made in a defective form. ...
introduction
introduction

... The 4 samples were simultaneously sequenced in one GS-FLX run using one 70X75mm PicoTiter plate device (Roche Diagnostics GmbH) and one GS LR-70 sequencing kit (Roche Diagnostics GmbH). Briefly, the 70X75mm Pico-Titer plate was divided in 4 regions using the Medium Regions Bead Loading Gasket (Roche ...
Barcode - Statistical Center for HIV/AIDS Research and Prevention
Barcode - Statistical Center for HIV/AIDS Research and Prevention

... Genetic Screens • The way a genetic screen is designed can profoundly influence which genes are uncovered • Different screen platforms yield different results (i.e. libraries, viruses, cell lines, transfection conditions and efficiencies, readouts) • Some weak hits may be the most important unlike ...
lab6
lab6

... • Specifying -maxw 20 makes MEME run faster since it does not have to consider motifs longer than 20. ...
Suracell: My Test Results
Suracell: My Test Results

... How do we know the efficiency of your genes in each category? Our DNA contains approximately 30,000 genes. Within our cells, our DNA is organized into 23 pairs of chromosomes. Genetic variations in our DNA are called SNPs (Single Nucleotide Polymorphisms). If we compare the DNA of two individuals, w ...
What is DNA?
What is DNA?

... Page 1 ...
Recombinant DNA cloning technology
Recombinant DNA cloning technology

... The enzyme recognizes a sequence that has two GC bp, two CG bp, one AT bp, and one TA bp in a particular order. Since genome has 40% GC, the chance of finding a GC or a CG pair is 0.2 while the chance of finding an AT or a TA pair is 0.3. The chance of finding the 6 base pairs in this sequence is: P ...
PCR
PCR

... Diabetes caused by mutations in the HNF1A (encoding hepatocyte nuclear factor-1 alpha) and GCK4 (encoding glucokinase 4) genes is one of the most common types of maturity onset diabetes of the young (MODY). HNF1α is a transcription factor that is important for the normal development of beta cells. M ...
AP BIOLOGY - Bremen High School District 228
AP BIOLOGY - Bremen High School District 228

... DNA polymerase follows the helicase so closely that there is no chance for the strands to come back together. Single-strand binding proteins bind the unwound DNA and prevent the double helix from reforming. The helicase pushes the two strands so far apart that they have no chance of finding each oth ...
THR_Paper2_CRISPR
THR_Paper2_CRISPR

... acquired immunity through recognizing and cutting exogenous genetic elements and inserting them into its own genetic code for future resistance. It is famously known for being the most powerful gene editing technology of our time. ...
Flip Folder 6 KEY - Madison County Schools
Flip Folder 6 KEY - Madison County Schools

... c. Single-strand binding proteins The 2 strands of a double helix bind together by hydrogen bonds between the bases. These bonds are essentially like magnets (weak bonds between opposing charges). Single strand binding proteins hold the single strands away from each other so they don’t reanneal behi ...
Ch. 5: Presentation Slides
Ch. 5: Presentation Slides

... • DNA fragments on a gel can often be visualized by staining with ethidium bromide, a dye that binds DNA • Particular DNA fragments can be isolated by cutting out the small region of the gel that contains the fragment and removing the DNA from the gel. • Specific DNA fragments are identified by hybr ...
No Slide Title
No Slide Title

... of an invariable gene product. rRNA and histone genes ...
Convergent evolution of antifreeze glycoproteins in
Convergent evolution of antifreeze glycoproteins in

... (notothenioid) or reduced number (Arctic cod) of spacer residues in the mature AFGPs indicate that their respective AFGP genes are transcribed and translated into large polyproteins that are posttranslationally cleaved. The near-identical AFGP protein structures from the two fishes have led to sugge ...
Lecture 10 Analyzing the DNA by array and deep sequencing (1)
Lecture 10 Analyzing the DNA by array and deep sequencing (1)

... Copy Number Aberration (CNA): ...
Molecular Biology and Evolution
Molecular Biology and Evolution

... FIG. 2.—A, phylogenetic analysis. The phylogenetic tree was inferred from the concatenated protein sequences of Cox1, Cox2, Cox3, Cob, Atp6 and Atp9 (Atp9 of animals was not included because it is nuclear encoded). Only unambiguously aligned portions of these protein sequences were used in the analy ...
Forward Genetic Screens: Strategies and challenges
Forward Genetic Screens: Strategies and challenges

... Amsterdam and Hopkins, 2006 ...
Click www.ondix.com to visit our student-to
Click www.ondix.com to visit our student-to

... detected in prokaryotes. Bacterial expression vectors are usually plasmids with strong promoters, ribosome-binding sites, and transcription terminators. Eukaryotic proteins are made by inserting cDNA into an expression vector. Also, antibodies can be used to screen clones from expression-vector cDNA ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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