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Gene Therapy: The Molecular Bandage for Treating Genetic Disorders
Gene Therapy: The Molecular Bandage for Treating Genetic Disorders

... hemophilia and thalassaemia), with genes being introduced into stem cells from the bone marrow, which give rise to all the specialized cell types in the blood. The strategy is to prepare a bone extract containing several billion cells, transfect these with a retrovirus-based vector, and then re-impl ...
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Chromosomal changes associated with changes in development

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File - Molecular Biology 2

... DNA samples but not from others, which means that the presence or absence of the amplified fragment is polymorphic in the population of organisms. In most organisms it is usually straightforward to identify a large number of RAPDs that can serve as genetic markers for many different kinds of genetic ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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