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Mining Single Nucleotide Polymorphisms from public sequence
Mining Single Nucleotide Polymorphisms from public sequence

... SNPs (single nucleotide polymorphisms) are abundant and useful genetic markers. Software exists to mine them from public data sets, but this doesn’t work in real time. GRID technology could help to deliver up-to-date alignments to users for any query sequence with putative SNPs marked up. Related us ...
Chloroplast DNA and Molecular Phylogeny
Chloroplast DNA and Molecular Phylogeny

... ring restriction fragment patterns and maps in order to study evolutionary relationships among different genera within a family (reviewed in refs. 7 and 8). Unfortunately, the phylogenetic insights gained in these studies have been necessarily limited by the small numbers of taxa examined. As mentio ...
A comparison of DNA quantification values obtained by
A comparison of DNA quantification values obtained by

... There are a range of methods available for the quantification of DNA including absorbance, agarose gel electrophoresis and fluorescent DNAbinding dyes. The traditional method involves measurement of the absorbance of the sample using a UV spectrophotometer. DNA has a maximal absorbance near 260 nm s ...
Week 6 Summary final
Week 6 Summary final

... • SDS and Molecular Radius – SDS-coated proteins are linear molecules, 18 Angstroms wide w/length proportional to their molecular weight. – Molecular radius and mobility in the gel is determined by side chains in AA sequence, and the length (i.e. molecular weight of the protein). – SDS-coated protei ...
The Murine Interleukin-3 Receptor a Subunit Gene
The Murine Interleukin-3 Receptor a Subunit Gene

... (Y subunit (GM-CSFRa) gene in the pseudoautosomal region of the X and Y chromosomes, the mlL-3Ra gene (113ra) is located in the proximal region of mouse chromosome 14, separated from the mouse GM-CSFRa gene, which is on chromosome 19. The mlL-3Ra gene spans about 10 kb and is divided into 12 exons. ...
Histones and histone-modifying enzymes
Histones and histone-modifying enzymes

... species, but these genes are more closely related to those identified in B. petri (Figure ...
Weighted gene co-expression network analysis (WGCNA) and
Weighted gene co-expression network analysis (WGCNA) and

... • WGCNA is also useful for inter-species comparison of gene expression levels • NEO can estimate edge orientation in a weighted gene coexpression network if relevant genetic marker data is available • NEO can also perform marker selection ...
A conserved repetitive DNA element located in the centromeres of
A conserved repetitive DNA element located in the centromeres of

... 44% GC and 56% AT. Sequence analysis did not reveal any special characteristics that often are associated with repetitive DNA families, such as internal short repeats, inverted repeats, and palindromes. ...
Solutions for Recombinant DNA Unit Exam
Solutions for Recombinant DNA Unit Exam

... 2. cDNA libraries lack the endogenous promoters found in the genome. 3. One may have multiple cDNAs for the same gene due to alternative splicing. 4. cDNA libraries will not contain genes that are not expressed in the cells used to generate the cDNA library. ii) You transform arg– bacterial cells wi ...
Final project
Final project

... Find 5 orthologous protein sequences for your favorite gene making sure that each sequence is coming from a different species. Extract FASTA sequences for these 5 proteins using the NCBI Entrez system, and get multiple alignments using CLUSTALW program. Copy and paste the colored output of CLUSTALW ...
Prof Martin`s extra notes
Prof Martin`s extra notes

... DNA Bending / Flexure Substructure can result in inherent bends in the duplex DNA and in bendability (which has a directionality to it). Very important element of recognition for many known protein-DNA complexes... Classic example: CAP (also known as CRP) transcription regulator. ...
Prediction of Effective genome size in metagenomics samples
Prediction of Effective genome size in metagenomics samples

... Read length also affects genome size inversely ...
Document
Document

... 4. Crime Scene Investigators search in areas of the genome that are unique from individual to individual and are “anonymous” (control no known trait or function) The areas examined are Short Tandem Repeats or STR’s ...
Genetics and Genomics in Medicine Chapter 1 Questions
Genetics and Genomics in Medicine Chapter 1 Questions

... ___1____ DNA, the G-rich strand has about 30 repeats that are ____5____. This short ____5____ overhang is able to bend backwards to base pair with complementary repeat sequences on the C-strand (after displacing the G-rich strand over a short region). The resulting structure, known as a ___6____ pro ...
HSA HW Packet #4
HSA HW Packet #4

... 1. A scientist cloned a goat. Which of these is a true statement about the cloned goat? A. It has new genes and traits. B. It lacks the genes for reproduction. C. It has genes that are identical to the original goat. D. It looks the same as the original goat but has different genes. 2. What is gel e ...
ppt for
ppt for

... varies among organs, lineages and chromosomes, owing to differences in selective pressures: transcriptome change was slow in nervous tissues and rapid in testes, slower in rodents than in apes and monotremes, and rapid for the X chromosome right after its formation.Although gene expression evolution ...
Chapter 16 Outline
Chapter 16 Outline

...  The mixtures were spun in a centrifuge, which separated the heavier bacterial cells in the pellet from lighter free phages and parts of phage in the liquid supernatant.  They then tested the pellet and supernatant of the separate treatments for the presence of radioactivity.  Hershey and Chase ...
Document
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... defined as time it takes for germ-line DNA to replicate i.e. from one gamete to the next Since most mutations occur at this point, rate of substitution under neutral theory is a function of both mutation rate and generation time: k = m/g General conclusion from molecular data is that the clock is ge ...
mRNA Codon
mRNA Codon

... Proteins are vital to living organisms. They are involved in chemical reactions, oxygen transport, muscle contraction, sensory perception, blood clotting, and many other activities. The great variety of roles requires equal variety in the structure of protein molecules. This variety is achieved by m ...
Inferring causal genomic alterations in breast cancer using gene
Inferring causal genomic alterations in breast cancer using gene

... (less than) or equal to the observed value if NS>0 (NS<0) ...
Diabetes mellitus
Diabetes mellitus

... crtI (carotene desaturase) from the soil bacterium Erwinia uredovora ...
Section 13.2 Summary – pages 341
Section 13.2 Summary – pages 341

... • Because DNA segments that are near each other on a chromosome tend to be inherited together, markers are often used as indirect ways of tracking the inheritance pattern of a gene that has not yet been identified, but whose approximate location is known. ...
Leukaemia Section t(5;14)(q33;q24) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(5;14)(q33;q24) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... 14q22.1 Note This gene is involved only in this translocation. DNA/RNA 31 exons spanning 111.3 Kb on 14q22.1. Transcription is from telomere to centromere. 4-5 alternative transcripts. Protein Homooligomer. Interacts with GSK3B (GSK3-beta) via its C-terminus domain, it also interacts with C14ORF166 ...
Introduction You are going to investigate a genetic disease that
Introduction You are going to investigate a genetic disease that

... has numerous alleles (i.e. numerous forms in which it can occur), making STRs highly informative for identifying individuals. In the lab there is also a pipetting robot and an automated DNA sequence analyzer to separate individual genotypes. Although this equipment makes life (relatively) easy the k ...
Alison Keiper - The Progress of Gene Therapy
Alison Keiper - The Progress of Gene Therapy

... treatment.    Gene  therapy  is  more  likely  to  be  successful  when  there  is  only  one   mutated  gene  as  the  root  of  a  disease,  but,  unfortunately,  some  of  the  most  common   diseases  like  heart  disease,  arth ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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