"Using Model Organism Databases (MODs)". In: Current - SGD-Wiki
... “Model organisms” are nonhuman organisms that are typically used for biological research. The resulting data can be used as a framework for the interpretation and understanding of similar data from humans or other medically or economically important species. Popular model organisms include budding y ...
... “Model organisms” are nonhuman organisms that are typically used for biological research. The resulting data can be used as a framework for the interpretation and understanding of similar data from humans or other medically or economically important species. Popular model organisms include budding y ...
The Differential Killing of Genes by Inversions in Prokaryotic Genomes
... from ftp://www.ncbi.nlm.nih.gov. The two pairs of genomes were chosen because, according to parameters of asymmetry in each of these genomes, the genes lying on the leading strand form a set distinct from the set of genes lying on the lagging strand. We have extracted amino acid sequences of ortholo ...
... from ftp://www.ncbi.nlm.nih.gov. The two pairs of genomes were chosen because, according to parameters of asymmetry in each of these genomes, the genes lying on the leading strand form a set distinct from the set of genes lying on the lagging strand. We have extracted amino acid sequences of ortholo ...
SHORT COMMUNICATION A Procedure for Isolating
... susceptible to EcoRI and BamHI and gives a limit digest restriction pattern similar to that from vegetative DNA. No attempt has been made to establish if there are minor differences. The mechanism by which spore lysis is achieved is not fully understood. Could & Hitchins (1963) and many others have ...
... susceptible to EcoRI and BamHI and gives a limit digest restriction pattern similar to that from vegetative DNA. No attempt has been made to establish if there are minor differences. The mechanism by which spore lysis is achieved is not fully understood. Could & Hitchins (1963) and many others have ...
Making sense of deep sequencing
... into the always controversial discussion of which technology is better and for what application. Therefore, the remainder of this review focuses on principles that are important and likely to remain so regardless of the particular way sequencing is performed. Get ready to sequence. What is a sequenc ...
... into the always controversial discussion of which technology is better and for what application. Therefore, the remainder of this review focuses on principles that are important and likely to remain so regardless of the particular way sequencing is performed. Get ready to sequence. What is a sequenc ...
Finding Regulatory Motifs
... Why (15,4)-motif is hard to find? • Goal: recover original pattern P from its (unknown!) instances: P1 , P2 , … , P20 • Problem: Although P and Pi are similar for each i (4 mutations for a (15,4) motif), given two different instances Pi and Pj, they may differ twice as much (4 + 4 = 8 mutations for ...
... Why (15,4)-motif is hard to find? • Goal: recover original pattern P from its (unknown!) instances: P1 , P2 , … , P20 • Problem: Although P and Pi are similar for each i (4 mutations for a (15,4) motif), given two different instances Pi and Pj, they may differ twice as much (4 + 4 = 8 mutations for ...
GDP-HiFi DNA Polymerase
... Conc. 1 U/μl Store at -20°C Description GDP-HiFi is a new recombinant enzyme with genetic modification for its amino acid sequence, which results 70 times better fidelity than Taq DNA polymerase and an extremely fast elongation rate (as fast as 15 seconds per kb). GDP-HiFi has higher stability at high ...
... Conc. 1 U/μl Store at -20°C Description GDP-HiFi is a new recombinant enzyme with genetic modification for its amino acid sequence, which results 70 times better fidelity than Taq DNA polymerase and an extremely fast elongation rate (as fast as 15 seconds per kb). GDP-HiFi has higher stability at high ...
Practical English (2)
... about imaginary people and events, rather than books about real people or event; if something is a fiction, it is not true, although people sometimes pretend that is is true. 2 gene, genetic: a gene is a part of a cell in a living thing which controls its physical characteristics, growth, and develo ...
... about imaginary people and events, rather than books about real people or event; if something is a fiction, it is not true, although people sometimes pretend that is is true. 2 gene, genetic: a gene is a part of a cell in a living thing which controls its physical characteristics, growth, and develo ...
Notions of Biochemistry and Molecular Biology Manipulating DNA
... brachydactyly (short fingers, 1905), congenital cataracts (1906), Huntington’s disease ...
... brachydactyly (short fingers, 1905), congenital cataracts (1906), Huntington’s disease ...
Slide 1
... And Wikipedia continues in this vein by describing 2 types of (IT-related) ontology: “A domain ontology (or domain-specific ontology) models a specific domain, or part of the world. It represents the particular meanings of terms as they apply to that domain.” (Wikipedia, 2008) “An upper ontology (o ...
... And Wikipedia continues in this vein by describing 2 types of (IT-related) ontology: “A domain ontology (or domain-specific ontology) models a specific domain, or part of the world. It represents the particular meanings of terms as they apply to that domain.” (Wikipedia, 2008) “An upper ontology (o ...
91.510_ch8_part2
... Functions can be assigned to proteins Disadvantages: Detects only pairwise protein interactions False-negative results (as for affinity chromatography) -- bait may be mislocalized -- transient interactions may be missed -- some complexes require special conditions -- bias against hydrophobic protein ...
... Functions can be assigned to proteins Disadvantages: Detects only pairwise protein interactions False-negative results (as for affinity chromatography) -- bait may be mislocalized -- transient interactions may be missed -- some complexes require special conditions -- bias against hydrophobic protein ...
Errors in the Code
... synthesized. Silent mutations are very useful in phylogenetics as we will see later in the course. Slide 5 Missense mutations occur when a base in the DNA is changed, resulting in a codon for a different amino acid. The resulting polypeptide has one incorrect amino acid in its sequence. These mutati ...
... synthesized. Silent mutations are very useful in phylogenetics as we will see later in the course. Slide 5 Missense mutations occur when a base in the DNA is changed, resulting in a codon for a different amino acid. The resulting polypeptide has one incorrect amino acid in its sequence. These mutati ...
Use of mass spectrometry-derived data to annotate nucleotide and
... spectrometry at the low levels usually available in biological experiments. The major reasons for this are: (i) some of the peptides derived from a given protein might not ionize well in the mass spectrometer and thus result in very small peaks; (ii) the peptides might be too large or too small for ...
... spectrometry at the low levels usually available in biological experiments. The major reasons for this are: (i) some of the peptides derived from a given protein might not ionize well in the mass spectrometer and thus result in very small peaks; (ii) the peptides might be too large or too small for ...
Positions, Beliefs and Values.indd
... The basic idea behind gene therapy is to add a normally functioning copy of a gene to cells of the affected tissue. This is often done using an engineered virus as a vector. Gene therapy will only work if the normal gene is delivered to a large number of correct cells –several million in fact. Once ...
... The basic idea behind gene therapy is to add a normally functioning copy of a gene to cells of the affected tissue. This is often done using an engineered virus as a vector. Gene therapy will only work if the normal gene is delivered to a large number of correct cells –several million in fact. Once ...
Drug resistance of bacteria commensal with Drosophila
... glycosidic bonds (50 µg/ml in food). Antibiotics were added as liquid food cooled below 50°C, from stock solutions in ethanol (o-phenyl phenol), methanol (tetracycline), or water (ceftriaxone, spectinomycin). The principal was to follow sharp reduction in the bacterial load with simultaneous exposu ...
... glycosidic bonds (50 µg/ml in food). Antibiotics were added as liquid food cooled below 50°C, from stock solutions in ethanol (o-phenyl phenol), methanol (tetracycline), or water (ceftriaxone, spectinomycin). The principal was to follow sharp reduction in the bacterial load with simultaneous exposu ...
Nucleic Acids: DNA and RNA - E
... The double-stranded DNA molecule has the unique ability that it can make exact copies of itself, or self-replicate. When more DNA is required by an organism (such as during reproduction or cell growth) the hydrogen bonds between the nucleotide bases break and the two single strands of DNA separate. ...
... The double-stranded DNA molecule has the unique ability that it can make exact copies of itself, or self-replicate. When more DNA is required by an organism (such as during reproduction or cell growth) the hydrogen bonds between the nucleotide bases break and the two single strands of DNA separate. ...
Tracking bacterial DNA replication forks in vivo by pulsed field gel
... E. coli strain AQ2, derived from isolate 15, was pulse labeled with 14C-thymidine at various times during amino acid starvation and after the start of synchronous DNA synthesis. Intact chromosomal DNA was prepared in agarose and digested with the restriction enzyme Not I. The resulting Not I fragmen ...
... E. coli strain AQ2, derived from isolate 15, was pulse labeled with 14C-thymidine at various times during amino acid starvation and after the start of synchronous DNA synthesis. Intact chromosomal DNA was prepared in agarose and digested with the restriction enzyme Not I. The resulting Not I fragmen ...
Kinoshita, T et al.
... the imprinted pattern of FWA expression in A. halleri [34]. Thus, in A. halleri at least, the tandem repeat structure, but not the SINE-related sequence, is dispensable for imprinting. The relationship between genomic imprinting and transposon insertion is of interest because in mammals the paternal ...
... the imprinted pattern of FWA expression in A. halleri [34]. Thus, in A. halleri at least, the tandem repeat structure, but not the SINE-related sequence, is dispensable for imprinting. The relationship between genomic imprinting and transposon insertion is of interest because in mammals the paternal ...
Bacterial Gene Swapping in Nature
... concern: rising resistance to antibiotics in disease-causing bacteria. It turns out that bacteria, which are single-cell organisms, often donate antibiotic-resistance genes to other species of bacteria in the human body. Understanding when and how this transfer occurs should help investigators devel ...
... concern: rising resistance to antibiotics in disease-causing bacteria. It turns out that bacteria, which are single-cell organisms, often donate antibiotic-resistance genes to other species of bacteria in the human body. Understanding when and how this transfer occurs should help investigators devel ...
Chapter 13
... moves. It goes into the complex as one doublestranded molecule, and emerges as two double-stranded molecules. ...
... moves. It goes into the complex as one doublestranded molecule, and emerges as two double-stranded molecules. ...
The Regulatory Region of the Larabinose Operon: Its Isolation on a
... The large-scale purification described in detail in Materials and Methods yields 15 pg of purified fragment containing ara regulatory region DNA. The length homogeneity of this DNA was estimated by electron microscopy and electrophoresis on polyacrylamide gels. Measurement of all molecules visible o ...
... The large-scale purification described in detail in Materials and Methods yields 15 pg of purified fragment containing ara regulatory region DNA. The length homogeneity of this DNA was estimated by electron microscopy and electrophoresis on polyacrylamide gels. Measurement of all molecules visible o ...
Identification and characterization of the Arabidopsis gene encoding
... and bilins. Although UROS genes have been cloned from many organisms, the level of sequence conservation between them is low, making sequence similarity searches difficult. As an alternative approach to identify the UROS gene from plants, we used functional complementation, since this does not requi ...
... and bilins. Although UROS genes have been cloned from many organisms, the level of sequence conservation between them is low, making sequence similarity searches difficult. As an alternative approach to identify the UROS gene from plants, we used functional complementation, since this does not requi ...
Some Natural Language Processing (NLP) Tasks
... Gene Expression Data • in addition to DNA sequence data, we also use expression data to make our parses • microarrays enable the simultaneous measurement of the transcription levels of thousands of genes genes/ sequence positions ...
... Gene Expression Data • in addition to DNA sequence data, we also use expression data to make our parses • microarrays enable the simultaneous measurement of the transcription levels of thousands of genes genes/ sequence positions ...
... Ribonucleic acids (RNA) are made of a long single chain composed from nucleotides. Each nucleotide consists of a base, a ribose, sugar and a phosphate group. The sequence of nucleotides allows RNA to encode genetic information. All cellular organisms use messenger RNA (mRNA) to carry the genetic inf ...