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slow-learners - WordPress.com
slow-learners - WordPress.com

... 5. What are the characteristics of a wind pollinated flowers? 6. Trace the development of a mature ovule from a megaspore mother cell/ 7. What is double fertilization? Explain. 8. Differentiate between monoecious and dioecious plants. Give an example of each. ...
Structure od DNA and replication
Structure od DNA and replication

... one strand becomingASthe leading strand, the other the Biology. Gnetic control of protein structure and function lagging strand. ...
06MicrobialGenetExamII
06MicrobialGenetExamII

... of cell division, there are only two copies of the plasmid per cell. Assuming that the plasmid is sorted randomly, calculate (or draw) how often the plasmid should be lost by chance each generation. (5pts) ...
synthesis Gene Cluster of Streptomyces clavuligerus
synthesis Gene Cluster of Streptomyces clavuligerus

... protein indicating that promoter sequences upstream of ca-ORF4 are expressed in E. coli and confirming that the gene encodes an ArgJ-homologous protein with ornithine N-acetyltransferase activity but no acetylornithinase activity. Therefore we confirm that in S. clavuligerus there are at least two g ...
Advances and Perspectives in Genetics of Congenital Thyroid
Advances and Perspectives in Genetics of Congenital Thyroid

... pathway which includes the complex DUOX1/DUOX2// DUOXA1/DUOXA2 [5-7]. The DUOX1 and DUOX2 genes encoding similar proteins that are inserted in the apical membrane of thyroid cells. These proteins are known as dual oxidase because they have both a peroxidase homology ectodomain (peroxidase-like domai ...
Study Guide
Study Guide

... trisomic; those that have received just one copy of a chromosome are said to be monosomic for the chromosome.  Fig 15.12 shows non-disjunction. This leads to trisomy of which the most common example is Down’s syndrome (an aneuploid condition-chromosome 21). You should understand how this happens.  ...
Evolution of cis-regulatory elements in duplicated genes of yeast
Evolution of cis-regulatory elements in duplicated genes of yeast

... mutations are more common than beneficial mutations and that many eukaryotic genes have modular regulatory regions, Lynch and Force suggested that subfunctionalization might be the dominant mode of duplicate evolution [5]. In its original formulation, the subfunctionalization scenario suggests that ...
Construction of a Genetic Linkage Map in Man Using Restriction Fragment Length Polymorphisms.
Construction of a Genetic Linkage Map in Man Using Restriction Fragment Length Polymorphisms.

... unusual inheritance, since the probe may be detecting sequences that, although homologous, are genetically unlinked. Another interesting possibility is that RFLPs could result from the activity of DNA modifying enzymes [20]. In this case, pedigree analysis would show us the linkage relationship of t ...
Detecting phenotype-specific interactions between
Detecting phenotype-specific interactions between

... • Represent differentially expressed genes, in a phenotype, and their biological functions as a matrix – vector space model with biological processes as column vectors • Find associations between pairs of biological processes • Compare these associations with the corresponding associations in the ab ...
Document
Document

... was performed as described by Church and Gilbert (1984), at 50°C overnight. Three washes were made in 2 x SSC, 0.1 % (w/v) sodium dodecyl sulfate (SDS) at room temperature (5 min each), followed by 1 x SSC, 0.1% SDS washes ...
Chapter 20
Chapter 20

... genes as well as many noncoding nucleotide sequences. • A scientist may only be interested in one small gene, so to work directly with specific genes, scientists prepare gene-sized pieces of DNA in identical copies, a process called DNA cloning ...
printer-friendly version of benchmark
printer-friendly version of benchmark

... Mutation is a term that is often associated with negative or unusual consequences. The media uses mutations as an entertaining theme to the plots of many shows such as Heroes, X-men, Spiderman, and Teenage Mutant Ninja Turtles. The main characters in these shows have undergone radical transformation ...
Hormona del Crecimiento y Dopaje Genético
Hormona del Crecimiento y Dopaje Genético

... Possible strategies for detection gene doping Screening • Detect plasmid vector sequences and Iimune response to viral vectors • Differentiate transgenic DNA from genomic DNA • Microarrays to detect changes in gene expression • Proteomics to detect changes in gene expression • DNA bar codes (difficu ...
Large-Scale Purification Of Plasmids pRIT4501 and - RIT
Large-Scale Purification Of Plasmids pRIT4501 and - RIT

... isopycnic points. The table also shows the density of phage λ DNA. After transferring the lysate to the ultracentrifuge tube, ethidium bromide is added. While ethidium bromide certainly facilitates collection of the DNA at the end of the centrifuge run by virtue of its fluorescence, it is actually a ...
DNA and Protein
DNA and Protein

... Not only did the DNA have to originate itself by random accident, but the translation machinery already had to be produced by accident—and also immediately! Without it, the information in the DNA could not be applied to the tissues. Instant death would be the result. “The code is meaningless unless ...
Genomic In Situ Hybridization (GISH) as a Tool to Identify
Genomic In Situ Hybridization (GISH) as a Tool to Identify

... Alien chromosome or chromosome segments were detected by the probe of H. californicus or H. angustifolius in their backcross progenies H. californicus was crossed with HA 410 and backcrossed several times to reduce the chromosome number close to the 2n=34 of the cultivated sunflower. When the chromo ...
Carcinomas with DNA Mismatch Repair Deficiency
Carcinomas with DNA Mismatch Repair Deficiency

... The DNA mismatch repair proteins are ubiquitously expressed in normal human tissues, particularly proliferating tissues, and nuclear expression in crypt epithelium and lymphocytes serves as an internal positive control for stain quality. In the setting of HNPCC, most hereditary and second-hit tumor ...
Nomenclature I
Nomenclature I

... Genetic Nomenclature for Mice, which states: “A locus is a point in the genome, identified by a marker, which can be mapped by some means. It does not necessarily correspond to a gene; it could, for example, be an anonymous non-coding DNA segment or a cytogenetic feature. A single gene may have seve ...
PDF
PDF

... to the conclusion that circular DNA persists and eventually may be amplified through early embryogenesis, but becomes degraded after the gastrula stage. However, it was also found that a small number of injected gene copies was integrated into the Xenopus genome and it was shown that such integrated ...
pdf
pdf

... regions to consider, with 51 unique flanking genes. There are 6 super-regions with at least 99 bp overlapping with ultra-conserved elements. At least one of the flanking genes for each of these 6 super-regions is a transcription factor located 1–314 kb away (IRX3, IRX5, IRX6, HOXD13, DMRT1, DMRT3, F ...
video slide
video slide

... fragments directly • Powerful computer programs are used to order fragments into a continuous sequence ...
pioneered
pioneered

... transmit pathogens in the laboratory doesn’t help people in the wider world. Even if scientists bred and released thousands of these transgenic mosquitoes, they wouldn’t have much effect on public health unless the genetically altered insects competed with (and eventually replaced) the local strains ...
retrovirus
retrovirus

... • Identification of the affected gene • A cDNA clone encoding the gene • A substantial disease burden and a favorable riskbenefit ratio • Sufficient knowledge of the molecular basis of the disease to be confident that the gene transfer will have the desired effect • Appropriate regulation of the gen ...
serious asthma should focus on the possible confounding role
serious asthma should focus on the possible confounding role

... genetic elements known as short and long interspersed nuclear elements, long terminal repeat-superfamilies and direct transposition of TE-containing genomic DNA. Formerly regarded as junk DNA, it is now becoming increasingly evident that TEs often function to regulate and fine tune gene expression [ ...
Senior General Knowledge - Famous Scientists
Senior General Knowledge - Famous Scientists

... 7. Francis Crick, and James Watson and Maurice Wilkins shared the 1962 Nobel Prize for Physiology or Medicine for solving the structure of DNA. 8. Rosalind Franklin (1920 – 1958) was a British biophysicist who is best known for her work on the molecular structures of coal and graphite, and X-ray dif ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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