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Variations in the correlation of Gene Ontology annotations with
Variations in the correlation of Gene Ontology annotations with

... probabilistic perspective. Two alternative views can be taken, i) that it is necessary to cover all annotations assigned to each gene (i.e. all genes have all assigned functions), or ii) that it is only necessary to cover some annotation made to each gene (i.e. alternative assignments can hold in di ...
Slides
Slides

... – Strongest evidence – However, will not be able to find species-specific ones ...
click here to file
click here to file

... CXCR4 Introduction • G protein coupled receptor (GPCR) • Activated by SDF-1, a chemokine • Thought to be important to two major diseases • HIV- Acts as co-receptor for M Tropic • Possible cure for M Tropic infections • Cancer- Expressed more in breast and other cancers • Aids in metastasis of cance ...
Active-site Determination of a Pyrimidine Dimer
Active-site Determination of a Pyrimidine Dimer

... One mechanism for the repair of UV-induced DNA damage is the base excision repair pathway. The initial step in this pathway and the speci®city for the type of damage that is to be repaired reside in DNA glycosylase/abasic (AP) lyases. Cleavage of the glycosyl bond of the 50 pyrimidine of a cyclobuta ...
manual genetic jigsaw v3.4 LR
manual genetic jigsaw v3.4 LR

... The origin of replication (ori) is the place where the plasmid DNA replication starts. As plasmids are self-replicating molecules, they borrow many of the proteins needed for replication from their bacterial host in a semi-parasitic way. However, as many plasmids offer a selective advantage to their ...
Document
Document

... • Very stringent = clean dot plot, little signal ...
Protein Synthesis
Protein Synthesis

... nucleus to translation of proteins on ribosomes in the cytoplasm. 4b. Students know how to apply the genetic coding rules to predict the sequence of amino acids from a sequence of codons in RNA. Time Length: 30 minutes Prerequisite Knowledge: Students know the basics of transcription and translation ...
12–1 DNA - cloudfront.net
12–1 DNA - cloudfront.net

... and phosphate groups of each nucleotide. The nucleotides can be joined together in any order. ...
Motifs and motif prediction methods I - BIDD
Motifs and motif prediction methods I - BIDD

... 7. Score that sequence with its new start position. 8. Compare this new score with its original score. 9. If newscore >= oldscore, then jump to that new start position, else jump to that new start position with probability = newscore ...
Isolation and Characterization of Mutations in the b-Tubulin Gene of Saccharomyces cerevisiae .
Isolation and Characterization of Mutations in the b-Tubulin Gene of Saccharomyces cerevisiae .

... transformation with 0 5 5 % of the volume of such a preparation of yeast DNA, selecting ampicillin resistance. For recovery of integrated plasmids, the yeast DNA was cleaved with the appropriate restriction enzyme, phenol extracted and ethanol precipitated and ligated at a concentration of 0.5 Pg/ml ...
presentation - Genome-to-Genome Distance Calculator
presentation - Genome-to-Genome Distance Calculator

... please find below the results of your GGDC job. A short explanation is found at the end of this e­mail. Further information is provided on our website at http://www.gbdp.org/species/. If you use this service in a publication, please cite the appropriate references listed ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 13. Describe the importance of flavones and flavanoids. 14. How is the presence of ES complexes determined? Explain. 15. Explain the different types of soil. 16. How is N-terminal of an amino acid determined by dansyl chloride method? 17. What is competitive inhibition? Explain with an example. 18. ...
unit II - SP College
unit II - SP College

... tandem arrays with approximately 16,000 copies per genome. Several RU sequences were cloned and sequenced to reveal conserved regions of conventional DNA sequences interspersed with four domains of microsatellite repeats biased in composition with purines on one strand and pyrimidines on the other, ...
Molecular Evolution
Molecular Evolution

... Thus, a protein in which the active sites constitute only 1% of its sequence will be less constrained, and therefore will evolve more quickly than a protein that devotes 50% of its sequence to performing specific biochemical or physiological tasks. ...
Brooker Chapter 14
Brooker Chapter 14

... Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
Modulation of base excision repair of 8
Modulation of base excision repair of 8

... decrease of fluorescence, compared with cells transfected with the reference plasmids harbouring the unmodified oligonucleotide (Figure 1B–F). The effect had the same magnitude for 8-oxoG located in the transcribed DNA strand and one in the complementary DNA strand. In cells, fixed 8 h after transfecti ...
Expression profiling reveals off
Expression profiling reveals off

... by single nucleotide changes in the siRNA sequence2,6, we would not have predicted that this limited degree of sequence similarity would be sufficient for transcript silencing. However, to test this possibility, we systematically substituted the nucleotide at each position of the siRNA sequence and ...
Supplementary Methods
Supplementary Methods

... mutant (nup145∆Cter) was generated by in-frame insertion of the protein A or the GFP coding sequence followed by a stop codon through homologous recombination in the genomic copy at amino acid position 606. The PCR product used was generated using oligonucleotides 113 and 114 (Table 2). GFP-tagged p ...
Restriction Enzyme digestion of DNA
Restriction Enzyme digestion of DNA

... it with EcoR1, you’ll get another cut. So every time EcoR1 recognizes this palindrome on your plasmid, it will cut through the DNA. So when it opens up the DNA, may get a couple of unpaired bases, and those unpaired bases are called sticky ends, and if you throw some nucleotides from different speci ...
The role of DNA shape in protein-DNA recognition
The role of DNA shape in protein-DNA recognition

... residue across all homeodomains, inserts into a narrow region formed by a 4-base-pair (bp) A-tract. An example of a long and very narrow A-tract that binds a2-Arg 7 from the MATa1–MATa2 complex with DNA is shown (Fig. 3b)17. In contrast, a2-Arg 4 inserts into a shallower region at one end of the A-t ...
Transcript for the LearnGenetics Simulation
Transcript for the LearnGenetics Simulation

... But DNA strands are molecules so tiny that you can’t see them even under most microscopes. Is there a way to sort and measure DNA strands in your tube even though you can’t see or touch them? There is! It’s called gel electrophoresis (pronounced ee-LEK-tro-fo-REE-sis) (Press FORWARD to continue) ...
Inferring Gene Regulatory Networks from Time
Inferring Gene Regulatory Networks from Time

... the measured data instead of created artificially. Bayesian networks, on the other hand, do not allow the existence of loops. Bayesian networks rely on the joint probability distribution of the estimated network to be decomposable in a product of conditional probability distributions. This decompos ...
Limitations of Pseudogenes in Identifying Gene Losses
Limitations of Pseudogenes in Identifying Gene Losses

... the v5.3 genome assembly and annotation to determine if any of these potential gene losses are simply due to gaps in the v4.3 assembly. The 114 query sequences were searched against the v5.3 D. melanogaster genome using BLASTn with the same criteria as before. Interestingly, 23 query genes hit very ...
Investigation of the premelanosome protein
Investigation of the premelanosome protein

... Re-sequencing of parts of the rabbit PMEL gene and identification of intronic polymorphisms To identify polymorphisms that could be useful to evaluate their possible co-segregation with the black-blue spotted phenotype in the F1 families, fragments of the rabbit PMEL gene including 4 out of 11 exons ...
Vertebrate Gene Cis-Regulation - A computational tour of the human
Vertebrate Gene Cis-Regulation - A computational tour of the human

... ~106 substrings do not code for protein What do they do then? [Science 2004 Breakthrough of the Year, 5th runner up] http://cs273a.stanford.edu [Bejerano Aut07/08] ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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