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Chapter 14 Notes - Gonzaga High School
Chapter 14 Notes - Gonzaga High School

... Mendel’s laws of independent assortment and segregation explain heritable variation in terms of alternative forms of genes that are passed along according to simple rule of probability. These laws apply not just to garden peas, but to all other diploid organisms that reproduce by sexual reproduction ...
Updated map of duplicated regions in the yeast genome
Updated map of duplicated regions in the yeast genome

... the amount of the genome placed into paired regions, under the assumption that the hypothesis that the entire genome was duplicated in a single event is correct. The core of the new map, with 52 pairs of regions containing three or more duplicated genes, is largely unchanged from our original map. 3 ...
What does PCR stand for?
What does PCR stand for?

... Due to osmosis, cells would burst before we could collect DNA ...
Genes can be switched on and off by the protein CTCF
Genes can be switched on and off by the protein CTCF

... protein with similar functions was examined, called BORIS. The fragments of DNA where CTCF was bound were identified using a method called PCR (polymerase chain reaction). The results showed that CTCF and BORIS could bind to the same target, and suggested they did so at different stages of developme ...
Supplementary Methods S2: Exome Sequencing
Supplementary Methods S2: Exome Sequencing

... SNVs from VarScan and SomaticSniper were merged into a single non-redundant file. To remove false positives from paralogous alignments, local mis-alignments, sequencing error, and other factors, we filtered SNVs to remove any with strand bias, read position bias, or multiple high-quality mismatches ...
Human Cytomegalovirus UL34 Early and late Proteins Are Essential
Human Cytomegalovirus UL34 Early and late Proteins Are Essential

... analyses of the HCMV genome. We extended their results by constructing and studying recombinant viruses using the bacterial artificial chromosome (BAC) that contains the HCMV AD169 genome, pHB5 [8]. HCMV-BACs that either entirely lacked UL34 (UL34), contained UL34 with a mutation in the ATG initiat ...
Matlab Tutorial: Bacterial gene expression
Matlab Tutorial: Bacterial gene expression

... to bias resulting from the level of fluorescence within each cell. Following the procedure outlined in the example on the cell division time in E. coli, once we have performed the thresholding, we will be left with a mask image with discrete regions that we identify as cells denoted by the different ...
Database Searches for similar sequences
Database Searches for similar sequences

... Blast III, Extending Hits • extend hits in both directions (with or without allowing gaps) • Residues will be added until the incremental score drops below a threshold (S) ...
CHAPTER 2 Genome Sequence Acquisition and
CHAPTER 2 Genome Sequence Acquisition and

... However, there is evidence that muscular dystrophy has a signaling component, and it might be possible that the dystrophin gene produces truncated proteins that might be functional DNA binding proteins. Therefore, we should not conclude that dystrophin does or does not have a transcriptional functio ...
Measurement of flowering time
Measurement of flowering time

... 2006). It remains to be shown whether any of the three tomato members of CO family group Ia plays a role in tomato flowering, since the results reported so far do not exclude this possibility (Ben-Naim et al., 2006). Whether other CO family genes from potato have a stronger effect on flowering is st ...
3333f00schedule
3333f00schedule

... FINAL EXAM: Monday, 10 December, 8:00 AM to 10:00 AM ...
Subfunctionalization: How often does it occur? How long does it take?
Subfunctionalization: How often does it occur? How long does it take?

Unit 9: DNA, RNA, and Proteins
Unit 9: DNA, RNA, and Proteins

... problem with DNA replication. • DNA polymerase III can only work in a 5’ → 3’ direction. The 5’ ends of each daughter DNA strand cannot be replicated. • DNA polymerase III needs to attach to a 3’ end in order to add new nucleotides • This is why RNA primase is required in DNA replication. • Since th ...
Form 1259i - Information about DNA testing for visa and citizenship
Form 1259i - Information about DNA testing for visa and citizenship

... complete and sign the client consent pro forma (DNA Laboratory to Disclose DNA Results to the Department of Immigration and Border Protection) that the department sent in the DNA testing offer letter (which you will also have to take to show the DNA sample collector). The client consent pro forma is ...
Open full article - About the Conference MendelNet 2016
Open full article - About the Conference MendelNet 2016

... determination in cannabis is complex and can even be reversed or modified by chemical treatment and environmental factors (Chailakhyan 1979, Mohan Ram and Sett 1979). At the same time, male flowers are able to develop on female plants under extreme conditions (Clarke 1997). BK8, although being a med ...
Chapter 8.qxp
Chapter 8.qxp

... would yield only 16 possible words, or “codons.” But triplet combinations produce 64 possible codons, which would be plenty. Little else was obvious at the time about how genes might be translated into proteins. Today we understand that gene sequences do use three-letter codons to specify individual ...
M-protein and other intrinsic virulence factors of Streptococcus
M-protein and other intrinsic virulence factors of Streptococcus

... transfer that help them compete in particular ecological niches. Genetic elements can be transferred on DNA stretches, within viruses, or by intercellular contacts. For example, bacteriophages carrying toxin genes can be inserted into bacterial chromosomes and re-program Streptococcus pyogenes to pr ...
BMC Genomics
BMC Genomics

... transfer that help them compete in particular ecological niches. Genetic elements can be transferred on DNA stretches, within viruses, or by intercellular contacts. For example, bacteriophages carrying toxin genes can be inserted into bacterial chromosomes and re-program Streptococcus pyogenes to pr ...
bioinformatics
bioinformatics

... ancestor. Two such segments are called segmental homologs (SH). When dealing with an incompletely mapped genome, knowing that two segments are homologous is useful in that it suggests that other (unmapped) features within those same segments may have homologous counterparts in the opposite segment. ...
Missense mutations in the PAX6 gene in aniridia.
Missense mutations in the PAX6 gene in aniridia.

... segmentation gene in Drosophila melanogaster.1-2 The encoded proteins are transcriptional regulators with DNA binding through a conserved domain consisting of 128 amino acids (paired box).3 Some Pax genes share another conserved domain, homeobox, which also provides DNA binding.4'5 The PAX6 gene has ...
Genetics Project
Genetics Project

...  Collects and passes out group materials and work  Communicates with the teacher  Assigns a ‘Daily Participation Grade’ for each member of the group  Collects and grades homework for the group when necessary ...
Lesson Overview
Lesson Overview

... How does DNA replication differ in prokaryotic cells and eukaryotic cells? In eukaryotic cells, replication may begin at dozens or even hundreds of places on the DNA molecule, proceeding in both directions until each chromosome is ...
The complete nucleotide sequence of the chick a
The complete nucleotide sequence of the chick a

... described by Maxam and Gilbert (lit) and the cleaved fragments fractionated on thin urea-acrylamide gels (17) which were autoradiographed with x-ray film (Kodak) in the presence of intensifying screens (Dupont, Cronex) at -70*C. Based on several sequence determinations of the same region using diffe ...
Comparative genomics exercises - Genome curation on emerging
Comparative genomics exercises - Genome curation on emerging

... (lines). For each genome, the top three frames show the forward orientation and bottom three frames show the reverse orientation. The black vertical lines in the each frame represent stop codons. * Right-click on a gene/exon  View  View Selected features to get information about that gene. * Use ...
Missense mutations in the PAX6 gene in aniridia.
Missense mutations in the PAX6 gene in aniridia.

... segmentation gene in Drosophila melanogaster.1-2 The encoded proteins are transcriptional regulators with DNA binding through a conserved domain consisting of 128 amino acids (paired box).3 Some Pax genes share another conserved domain, homeobox, which also provides DNA binding.4'5 The PAX6 gene has ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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