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Drug-specific Sites of Topoisomerase II DNA
Drug-specific Sites of Topoisomerase II DNA

... DNA topology through concerted DNA breakage and religation ac tivities (3). An important function of DNA topoisomerasesconsists in relieving the torsional stress generated during DNA replication and transcription (4). Type H DNA topoisomerase has an essential role in the decatenation ofdaughter DNA ...
PTC Day 2 Powerpoint
PTC Day 2 Powerpoint

... PCR amplified 221 base pairs of DNA from the PTC ...
(Pierce, 2014). Figure 3. a gel after PCR reaction. L refers to which
(Pierce, 2014). Figure 3. a gel after PCR reaction. L refers to which

... Figure 2. G418 plasmid contains EGFP and ampicillin ...
Guide to Scoring the National Pre-Build Model
Guide to Scoring the National Pre-Build Model

... 4. Alpha  helices  are  right-­‐handed  (2.5  pts,  0.5  pts  each).  In  order  to  receive  the  points,  check  that  the  alpha  helices   in  the  model  are  right  handed  as  shown  in  the  figure  above  and  to  the  ri ...
BIOL4 - gale-force-glyn
BIOL4 - gale-force-glyn

... such as light, temperature, humidity and air movement affect transpiration? ...
Module 1: Introduction
Module 1: Introduction

... GO Structure • Terms are related within a hierarchy – is-a – part-of ...
101. The Role of Rigidity in DNA Looping
101. The Role of Rigidity in DNA Looping

... protein with sufficient flexibility to bind DNA half-sites with a variety of spacings and orientations (21), and is likely to provide an unstructured connection between the AraC DNA binding domains (Fig. 2). The presence of long repeated sequences coding for the two DNA binding domains could generat ...
Genomic Analysis of Arabidopsis Thaliana
Genomic Analysis of Arabidopsis Thaliana

... Bowman J. (1993). Arabidopsis, An Atlas of Morphology and Development. New York, NY: SpringerVerlag. 450 pp. [The growth and development of Arabidopsis is introduced with numerous pictures of the wild type and many mutants. The arrangement of various aspects of continuous morphogenesis in plants mak ...
Comparison between Human and Mouse genomes
Comparison between Human and Mouse genomes

... Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in g ...
Copying our Genes
Copying our Genes

... Housed in the nucleus of your cells, DNA holds the key to your genetic destiny. The DNA code tells an amazing story about the person you will grow up to be. Each gene controls production of a protein, which ultimately affects a trait in your body. The tools of modern day molecular biology give scien ...
Ontologies
Ontologies

... parents. Hint: In a simple hierarchy, seed was always a part of fruit, but Ontology can represent its multiple lineages. Answer: The two immediate parents are whole plant (GRO:0005000) and fruit (GRO:0006009). ...
Programmed Materials Synthesis with DNA
Programmed Materials Synthesis with DNA

... possess flexible junctions,49 the geometric assignment relies on topology and not actual geometry. In principle, by controlling the number of “arms” in a given branched DNA structure, the connectivities of more complex DNA structures can be controlled. However, thus far it has proven very difficult ...
No Slide Title
No Slide Title

... expression pattern (knock-in) • advantages – can generate a true loss-of-function alleles – precise control over integration sites – prescreening of ES cells for phenotypes possible – can also “knock in” genes • disadvantages – not trivial to set up – may not be possible to study dominant lethal phe ...
Characterization of Two Rice MADS Box Genes That Control
Characterization of Two Rice MADS Box Genes That Control

... protein (Figs. 1 and 2). This region is the most conserved region as observed from other MADS box genes. The second conserved domain, the K box, is located between the residues 95 and 160 in both OsMADS7 and OsMADS8 (Figs. 1 and 2) . The genes contain two variable regions, the I region between the M ...
(FA-SAT) in a Cat Fibrosarcoma Might Be Related to Chromosomal
(FA-SAT) in a Cat Fibrosarcoma Might Be Related to Chromosomal

... marker chromosomes, could be essential for centromere function, thus leading to the retention of these variable marker chromosomes. The presence of blocks of satellite DNA sequences in other regions of the marker chromosomes might be the result of chromosomal rearrangements. All these suppositions, ...
(S) tet Resistance Determinant Element Containing the Tetracycline
(S) tet Resistance Determinant Element Containing the Tetracycline

... initially identified in a multiresistant Listeria monocytogenes strain on a 37-kb conjugative plasmid, pIP811 (2). Subsequently tet(S) has been found on plasmid pK214 from Lactococcus lactis (8) and in the chromosome of Enterococcus faecalis (3). The tet(S) gene in L. lactis and L. monocytogenes is ...
Identification of Specific DNA Binding Residues in the
Identification of Specific DNA Binding Residues in the

... of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India ...
Gene targeting by hybridization-hydrolysis process
Gene targeting by hybridization-hydrolysis process

... A new technology used to specifically target any transcript from a complex population of single-strand cDNA molecules was applied to dramatically decrease the abundance of selected genes in cDNA libraries. This innovative procedure offers new alternatives to previous efforts focused on normalizing t ...
The Macaque Genome: Lessons from Comparative
The Macaque Genome: Lessons from Comparative

... about the relatedness among organisms and the traits that make them similar or different, can often be inferred by examining them at the DNA level, i.e., by looking at their DNA sequences. Comparing the DNA sequence of our own genome to other animal genomes (a field of study known as comparative gen ...
Poster - Pacific Biosciences
Poster - Pacific Biosciences

... As a cost-effective alternative to whole genome human sequencing, targeted sequencing of specific regions, such as exomes or panels of relevant genes, has become increasingly common. These methods typically include direct PCR amplification of the genomic DNA of interest, or the capture of these targ ...
Mutation, Transposition, and Recombination
Mutation, Transposition, and Recombination

... overlaps the plot for best fitness. This indicates that the populations lost all the diversity and all the individuals have the same genetic makeup. Obviously, if populations converge to this stage before finding a good solution, they become irrevocably stuck in that point if no other, nonrecombinat ...
Slide 1
Slide 1

... protein functional in a new way So, now we have a genome that can do all the ‘old stuff’ (with the original gene), but it can now do something NEW. Selection may favor these organisms. ...
Genome Projector: zoomable genome map with multiple views
Genome Projector: zoomable genome map with multiple views

... which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ...
The Amino Terminus of the Yeast F1-ATPase B
The Amino Terminus of the Yeast F1-ATPase B

Mei-S332, a Drosophila Protein Required for Sister
Mei-S332, a Drosophila Protein Required for Sister

... DNA, we found that the four transcript forms arise by alternative splicing and polyadenylation (data not shown). Despite differences in processing of the S’and S’untranslated regions in the different mei-S332 transcripts, all four cDNAs sequenced share the identical long open reading frame and thus ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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