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I n o v
I n o v

... The immobilization and hybridization of the DNA probe on the nano-Au–CNT/PANnano films were investigated with differential pulse voltammetry (DPV) and cyclic voltammetry (CV) using methylene blue (MB) as indicator, and electrochemical impedance spectroscopy (EIS) using [Fe(CN)6]3/4 as redox probe. ...
Virus induced gene silencing, a post transcriptional gene silencing
Virus induced gene silencing, a post transcriptional gene silencing

... Therefore, a number of viral-derived vectors have been developed (Table 1) and many procedures have been optimized by the researchers. TRV system was efficiently optimized for efficient silencing of Solanaceous plants [14; 15], and the system was also applied for tomato to study role of fruit ripeni ...
References - Plant Developmental Biology
References - Plant Developmental Biology

... progenitors in the embryo and apical meristems. Our primary goal is to gain experience in the interpretation of the mature structure of wild-type Arabidopsis plants (and the developmental basis for that structure) as a baseline for future comparison with mutant phenotypes. In this lab we will use se ...
Life Sciences P2 GR 12 Exemplar 2014 Eng Memo
Life Sciences P2 GR 12 Exemplar 2014 Eng Memo

...  Repeat the investigation  Take many samples each time and calculate the average mortality (Mark first TWO only) (any 2) ...
The Mobile Genetic Element Alu in the Human Genome
The Mobile Genetic Element Alu in the Human Genome

... in their own retroposition. Being transcribed by RNA polymerase III from internal promoters allows SINEs to remain transcriptionally active even after retroposition to new chromosomal sites. The best characterized LINE is the L1 family (Rogers 1985), which is present in excess of 50,000 copies inter ...
Recent Statistical Approaches
Recent Statistical Approaches

... active with novel analysis strategies and tools being published weekly”, and the value of many of these methods is questionable. Some results produced by using these methods are so anomalous that a breed of ‘forensic’ statisticians (Ambroise and McLachlan, 2002; Baggerly et al., 2003) who doggedly d ...
Recessive mutations
Recessive mutations

... Characteristics of Mutations at the DNA Level • Expanding Trinucleotide Repeats – may arise as a result of formation of hairpin structures during DNA replication – could also be due to unequal crossing over when repeated regions do not align properly ...
bb2013_03 - Territory Stories
bb2013_03 - Territory Stories

... attached to the skull. The size of scurs is quite variable; ranging from small scabs to growths about 15 cm long, although they are usually short). The gene for scurs is transmitted separately to the horn/poll gene and so has no effect on the presence or absence of horns. Scurs can only be seen in a ...
telomeres - Atlas of Genetics and Cytogenetics in Oncology and
telomeres - Atlas of Genetics and Cytogenetics in Oncology and

... Protection from cellular exonucleases Protection from non-homologous end joining Allow cells to differentiate between natural chromosome ends and damaged DNA Preserves integrity of chromosomes by allowing replication to occur without loss of coding sequences ...
Migration and mutation
Migration and mutation

... [2] A fixed number of migrant genes comes from the mainland to the island. A migration rate of m then corresponds to a migration of 2Ne m individual genes from the mainland to the island. This looks superficially the same as [1] but it isn’t the same. Each of these genes is sampled, rather than a fi ...
Epigenetic Inactivation of Chalcone Synthase-A
Epigenetic Inactivation of Chalcone Synthase-A

... C002 plants were treated with 5-azacytidine (Fig. 3C) or trichostatin A (Fig. 3D). The frequencies of cytosine methylation at CpG/CpNpG/CpNpN sites were reduced to 62.9%/55.8%/15.5% and 44.1%/46.1%/13.0% by treatments with 5-azacytidine and trichostatin A, respectively. The CaMV 35S promoter contain ...
Gene Section JUN (V-Jun sarcoma virus 17 oncogene homolog (avian))
Gene Section JUN (V-Jun sarcoma virus 17 oncogene homolog (avian))

... been demonstrated in several experimental systems. However, evidence indicating an anti-apoptotic activity of JUN has also been provided by the fact that deficiency of Jun gene causes massive hepatocyte apoptosis. The potential oncogenic transformation of JUN has been revealed by overexpression expe ...
Document
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... Using a parent DNA strand, adds free-floating nucleotides (A, T, G, & C’s) covalently to the new strand being constructed. ...
DINE-1 - Biological Sciences
DINE-1 - Biological Sciences

... chromosome, we are constructing a physical map of the banded portion. Our initial efforts focused on assembling cosmid clones into contigs, but several regions were not represented in our libraries which. We have recently begun screening a new genomic library constructed using a Bacterial Artificial ...
uniprotkb-goa_aug2011
uniprotkb-goa_aug2011

... • Determines which GO terms are more often associated with a specified list of genes/proteins compared with a control list or rest of genome • Many tools available to do this analysis • User must decide which is best for their analysis ...
Human Apolipoprotein A-l-C-lll Gene Complex is Located on
Human Apolipoprotein A-l-C-lll Gene Complex is Located on

... related DNA sequences have been described for the globins, immunoglobulins, histocompatibility antigens, ribosomal proteins, myosin heavy chain, interferon, and other genes. Mapping of these families revealed that some were widely dispersed in the genome, whereas others, perhaps for functional reaso ...
CHAPTER 9
CHAPTER 9

... are allowed to mate, and then the mating is interrupted at various time points. The interruption occurs by agitation of the solution in which the bacteria are found. This type of study is used to map the locations of genes. It is necessary to interrupt mating so that you can vary the time and obtain ...
PartFourAnswers.doc
PartFourAnswers.doc

... Increasing the distance between sequence 1 (encoding the trp leader peptide) and sequence 2 will decrease attenuation under conditions of high [Trp]. In this situation, the ribosome, after completing translation of the leader, will not cover sequence 2. Hence the 2:3 stem-loop can form, preventing f ...
Water at DNA surfaces: Ultrafast dynamics in minor groove recognition
Water at DNA surfaces: Ultrafast dynamics in minor groove recognition

... ydration of DNA plays important role in its structure, conformation, and function. Of significance to the function is the selective recognition by DNA of small molecules (ref. 1 and references therein). X-ray crystallography, NMR, dielectric relaxation, and molecular dynamics simulation studies have ...
Epigenetics in mood disorders
Epigenetics in mood disorders

... Eric Nestler and colleagues have experimental documentation of the associations between histone modifications and changes in behavioral function in response to antidepressant treatment and ECS in the hippocampus of rodents, a brain region implicated in depression [8, 36, 37]. In mice subjected to ch ...
continued
continued

... – Transcription of a gene produces a very long RNA strand that contains introns and exons – This long strand, which extends beyond the first and last exons, is often called precursor mRNA, or premRNA – More nucleotides are added at the beginning and end of the pre-mRNA molecule, forming a “cap” and ...
Communique of GTTAC Meeting 18 September 2003 and 28
Communique of GTTAC Meeting 18 September 2003 and 28

... Liberty® cotton is tolerant to the herbicide glufosinate ammonium (also called phosphinothricin), the active constituent of herbicides Basta® and Liberty® (hence the name Liberty® cotton). It is expected that use of Liberty® cotton plants will allow more effective weed control in cotton crops by all ...
Communique of GTTAC Meeting 18 September 2003 and 28
Communique of GTTAC Meeting 18 September 2003 and 28

... Liberty® cotton is tolerant to the herbicide glufosinate ammonium (also called phosphinothricin), the active constituent of herbicides Basta® and Liberty® (hence the name Liberty® cotton). It is expected that use of Liberty® cotton plants will allow more effective weed control in cotton crops by all ...
shERWOOD-UltramiR shRNA
shERWOOD-UltramiR shRNA

... To benchmark the shERWOOD algorithm design against the early generation TRC and Hannon Elledge (GIPZ) shRNA designs, the Hannon lab (Knott et al 2014) performed a large scale screen using each of these designs to target 2200 genes that were likely to impact growth and survival based on gene ontology ...
Part 1: Have Your DNA and Eat It Too!
Part 1: Have Your DNA and Eat It Too!

... 1. Create a 9 base DNA sequence and record it in your notebook. You will use this sequence to create a DNA strand that is nine bases long. You must use at least one of each base. You can choose any order with a few exceptions. You are NOT allowed to choose the following 3 bases in order: ATC, ATT, A ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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