Mutations in FUS, an RNA Processing Protein, Cause Familial
... or H517Q FUS/TLS–GFP fusion proteins. Immunoblotting of fractions followed by immunostaining with an antibody to GFP demonstrated a substantially higher ratio of soluble cytosolic to soluble nuclear FUS/TLS for both mutants (Fig. 3B). Additionally, a higher ratio of total insoluble to soluble nuclea ...
... or H517Q FUS/TLS–GFP fusion proteins. Immunoblotting of fractions followed by immunostaining with an antibody to GFP demonstrated a substantially higher ratio of soluble cytosolic to soluble nuclear FUS/TLS for both mutants (Fig. 3B). Additionally, a higher ratio of total insoluble to soluble nuclea ...
Reactome Functional Interaction (FI) Network
... Analysis Workflow – Cancer Microarray Experiment: Gene expression profiling of pancreatic tumour (vs normal tissue). Output: list of genes that show significant changes in expression. Downstream analysis: 1. Validation of the top genes sorted by fold-change (or p-value): artificial cut-off, informa ...
... Analysis Workflow – Cancer Microarray Experiment: Gene expression profiling of pancreatic tumour (vs normal tissue). Output: list of genes that show significant changes in expression. Downstream analysis: 1. Validation of the top genes sorted by fold-change (or p-value): artificial cut-off, informa ...
Temperature-dependent expression of virulence genes in fish
... 2010; Steinmann and Dersch, 2013). In addition, bacteria also have response mechanisms to drastic changes in temperature. The paradigms of this adaptation are the cold and heat shock response systems under which the bacteria induce a fast response to sudden and extreme temperature shift (Guisbert et ...
... 2010; Steinmann and Dersch, 2013). In addition, bacteria also have response mechanisms to drastic changes in temperature. The paradigms of this adaptation are the cold and heat shock response systems under which the bacteria induce a fast response to sudden and extreme temperature shift (Guisbert et ...
secondary school improvement programme - Sci
... individual expresses both traits, example: blood groups. Protein A and protein B are coded by alleles A and B. If no protein A or B is present, then these cells will be coded by the allele O. This means that there are three possible alleles for this one gene locus in humans. When there are more than ...
... individual expresses both traits, example: blood groups. Protein A and protein B are coded by alleles A and B. If no protein A or B is present, then these cells will be coded by the allele O. This means that there are three possible alleles for this one gene locus in humans. When there are more than ...
Exploring Important Biological Concepts Using Biology Workbench
... Enzymes function in a lock and key manner in that there is a complementary fit between an active site on the enzyme and the substrate. Missense mutations that affect the active site are more likely to alter function than mutations affecting other regions of the molecule, so residues in the active si ...
... Enzymes function in a lock and key manner in that there is a complementary fit between an active site on the enzyme and the substrate. Missense mutations that affect the active site are more likely to alter function than mutations affecting other regions of the molecule, so residues in the active si ...
Gradzial-Variety Development - California Cling Peach Board
... integrity defines the processing and fresh market peach industries. In order to achieve the durable fruit textures needed for bulk handling, the processing peach industry almost exclusively utilizes the nonmelting/clingstone peach rather then the melting/freestone peach used for fresh market. The cl ...
... integrity defines the processing and fresh market peach industries. In order to achieve the durable fruit textures needed for bulk handling, the processing peach industry almost exclusively utilizes the nonmelting/clingstone peach rather then the melting/freestone peach used for fresh market. The cl ...
A natural recessive resistance gene against potato virus
... PVY-0 and -1 but is overcome by PVY-1,2. Further studies revealed another characteristic feature of potyvirus resistance genes: resistance is often not restricted to a single potyvirus. Thus, pvr22 is also associated with resistance to a second potyvirus, the TEV. Whether a single locus or two tight ...
... PVY-0 and -1 but is overcome by PVY-1,2. Further studies revealed another characteristic feature of potyvirus resistance genes: resistance is often not restricted to a single potyvirus. Thus, pvr22 is also associated with resistance to a second potyvirus, the TEV. Whether a single locus or two tight ...
Journal of Medical Genetics: Large
... In the present study, we have identified CACNA1A rearrangements in 8 patients. Although, we have not undertaken fine mapping of these rearrangements to assess which predict translational frameshift, a number of points indicate they are likely to be pathogenic, not least their absence in a panel of ...
... In the present study, we have identified CACNA1A rearrangements in 8 patients. Although, we have not undertaken fine mapping of these rearrangements to assess which predict translational frameshift, a number of points indicate they are likely to be pathogenic, not least their absence in a panel of ...
Becker Muscular Dystrophy (BMD)
... Boys with BMD can sometimes be slow to learn to walk. Symptoms usually occur later in childhood, beginning with cramps during exercise. Most people with BMD are not very athletic in childhood and may struggle with sport at school. In early adulthood, it may become difficult to walk quickly, run, cli ...
... Boys with BMD can sometimes be slow to learn to walk. Symptoms usually occur later in childhood, beginning with cramps during exercise. Most people with BMD are not very athletic in childhood and may struggle with sport at school. In early adulthood, it may become difficult to walk quickly, run, cli ...
Thyroid Nodules
... • It increases the risk of benign and malignant thyroid nodules,associated with multicentric cancers, and shifts the incidence of thyroid cancer to an earlier age group • Children seem more predisposed to the effects of radiation than adults ...
... • It increases the risk of benign and malignant thyroid nodules,associated with multicentric cancers, and shifts the incidence of thyroid cancer to an earlier age group • Children seem more predisposed to the effects of radiation than adults ...
Point Mutation Analysis of PMP22 in Patients Referred for Hereditary
... splice site) and c.434delT (protein reading frame shift) mutations are clearly deleterious. The remaining base change, c.79-13T>A, is potentially deleterious based on the in silico data, but RNA analysis would be required to prove causality, and at present we have been unable to obtain an RNA sample ...
... splice site) and c.434delT (protein reading frame shift) mutations are clearly deleterious. The remaining base change, c.79-13T>A, is potentially deleterious based on the in silico data, but RNA analysis would be required to prove causality, and at present we have been unable to obtain an RNA sample ...
December 8, 2006 - Brandeis University
... (Beckingham, 2005). A Gal 4 P element, for instance, can express any gene of interest after being inserted into genomic DNA and activating a promoter to control a gene (Beckingham, 2005). Employing Gal4, the Ptpmeg gene was over-expressed in an attempt to determine which gene is responsible for the ...
... (Beckingham, 2005). A Gal 4 P element, for instance, can express any gene of interest after being inserted into genomic DNA and activating a promoter to control a gene (Beckingham, 2005). Employing Gal4, the Ptpmeg gene was over-expressed in an attempt to determine which gene is responsible for the ...
somatic hypermutation of the 5' noncoding region of the Frequent MARTINOrrI*t,
... fraction of malignant cells in the pathologic specimen was at least 70% as determined by cytofluorimetric analysis of cellsurface markers and/or rearrangement analysis of antigen receptor genes (17). Genomic DNA was isolated from the tumors and the BCL6 genomic configuration was assessed as ...
... fraction of malignant cells in the pathologic specimen was at least 70% as determined by cytofluorimetric analysis of cellsurface markers and/or rearrangement analysis of antigen receptor genes (17). Genomic DNA was isolated from the tumors and the BCL6 genomic configuration was assessed as ...
Ethnic differences in prostate cancer
... Measures of PSA level and tumour differentiation from the time of diagnosis and before commencing any treatment were identified. A Delphi sub-study investigated the appropriate use of diagnostic investigations and management strategies. Four urologists and one oncologist working in the PROCESS study ...
... Measures of PSA level and tumour differentiation from the time of diagnosis and before commencing any treatment were identified. A Delphi sub-study investigated the appropriate use of diagnostic investigations and management strategies. Four urologists and one oncologist working in the PROCESS study ...
Biocurator_Training_3
... You cannot mess up GONUTS by making lots of protein pages! Feel free to make a protein page on GONUTS, even if you don’t end up annotating it. ...
... You cannot mess up GONUTS by making lots of protein pages! Feel free to make a protein page on GONUTS, even if you don’t end up annotating it. ...
A Genetic Linkage Map of Mouse Chromosome 10
... a nick translation kit (Amersham) for whole plasmids or a multiprime DNA labeling kit (Amersham) for gel-purified fragments. Southern blot filters hybridized with 32P-labeled probes were routinely washed three times in 0.5 X SSCP, 0.1 % SDS for 30 min/wash at 65 O with the following exceptions. Sout ...
... a nick translation kit (Amersham) for whole plasmids or a multiprime DNA labeling kit (Amersham) for gel-purified fragments. Southern blot filters hybridized with 32P-labeled probes were routinely washed three times in 0.5 X SSCP, 0.1 % SDS for 30 min/wash at 65 O with the following exceptions. Sout ...
Yet viruses cannot be included in the tree of life - Université Paris-Sud
... viruses; the distinction is important in this debate). If virus-like implies self-replicating elements, we might agree, but if it implies genetic elements that are dependent for replication on other systems (what viruses actually are), we disagree. It would seem more logical that life derives from t ...
... viruses; the distinction is important in this debate). If virus-like implies self-replicating elements, we might agree, but if it implies genetic elements that are dependent for replication on other systems (what viruses actually are), we disagree. It would seem more logical that life derives from t ...
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... expression was first investigated in day-13.5 embryos (Fig. 2). In wild-type embryos Pax-1 was expressed along the entire axis in the region surrounding the anlagen of the vertebral bodies, the pedicles of the neural arches and the proximal parts of the ribs. In addition, Pax-1 expression was observ ...
... expression was first investigated in day-13.5 embryos (Fig. 2). In wild-type embryos Pax-1 was expressed along the entire axis in the region surrounding the anlagen of the vertebral bodies, the pedicles of the neural arches and the proximal parts of the ribs. In addition, Pax-1 expression was observ ...
Chapter 12 Using Chromosome Kits to Assemble Human Karyotypes
... First, count the chromosomes on the Biophoto® Sheets; the normal number is 46. The trisomy 21 Down syndrome has 47 chromosomes. The extra chromosome is a 21st and is caused by nondisjunction (homologous chromosomes that do not separate during meiosis). The 14/21 and 21/21 translocation carriers have ...
... First, count the chromosomes on the Biophoto® Sheets; the normal number is 46. The trisomy 21 Down syndrome has 47 chromosomes. The extra chromosome is a 21st and is caused by nondisjunction (homologous chromosomes that do not separate during meiosis). The 14/21 and 21/21 translocation carriers have ...
Translational control of regA, a key gene controlling
... to cure the Reg phenotype. The constructs tested were all modified versions of the plasmid pVcRegA1 (Kirk et al., 1999), which contains the native regA locus (Fig. 2) and which was also used as the positive control for all the transformation experiments reported here. In each mutant construct one of ...
... to cure the Reg phenotype. The constructs tested were all modified versions of the plasmid pVcRegA1 (Kirk et al., 1999), which contains the native regA locus (Fig. 2) and which was also used as the positive control for all the transformation experiments reported here. In each mutant construct one of ...