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1 How DNA Makes Stuff
1 How DNA Makes Stuff

... Once the transcription factors are in place, transcription can begin. The workhorse for this process is a collection of enzymes called RNA polymerase. There are a few of these, but the one most intimately connected with the process of making proteins is called RNA polymerase II (also called RNAP II ...
CIS 595 Bioinformatics
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... transcription alone (sometimes referred to as the primary transcript) would contain both coding (exon) and noncoding (intron) sequences. Before it can be translated into protein, the two ends of the RNA are modified, the introns are removed by an enzymatically catalyzed RNA splicing reaction, and th ...
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NCEA Level 2 Biology (91159) 2015
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large bases - De Anza College
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BDOL Interactive Chalkboard
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... that binds to an operator DNA sequence to prevent RNA polymerase from binding. • Same as in lac operon. • However, tryptophan acts as a co-repressor, not an inducer. That is, the repressor protein only binds to the operator if tryptophan is also bound to the repressor. • The repressor is coded for b ...
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... cDNA was then purified with the QIAquick PCR purification kit (Qiagen). This elution of about 55 ul of purified cyaninelabeled cDNA was stored at 4 C, and used in less than 24 hours. Hybridization: For each competitive hybridization, the labeled target cDNAs from two samples were used. One cDNA was ...
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Access Slides
Access Slides

... Activation of the HNF-4 Gene during CaCo-2 Cell Differentiation and Mapping of the Upstream Regulatory Region. (A) Total RNAs prepared from CaCo-2 cells at the indicated hours after reaching confluence were analyzed by RT-PCR using specific primers HNF-4, Enh-3’, Int.1, and ARP PO as control. Quan ...
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Messenger RNA



Messenger RNA (mRNA) is a large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence of the protein products of gene expression. Following transcription of primary transcript mRNA (known as pre-mRNA) by RNA polymerase, processed, mature mRNA is translated into a polymer of amino acids: a protein, as summarized in the central dogma of molecular biology.As in DNA, mRNA genetic information is in the sequence of nucleotides, which are arranged into codons consisting of three bases each. Each codon encodes for a specific amino acid, except the stop codons, which terminate protein synthesis. This process of translation of codons into amino acids requires two other types of RNA: Transfer RNA (tRNA), that mediates recognition of the codon and provides the corresponding amino acid, and ribosomal RNA (rRNA), that is the central component of the ribosome's protein-manufacturing machinery.The existence of mRNA was first suggested by Jacques Monod and François Jacob, and subsequently discovered by Jacob, Sydney Brenner and Matthew Meselson at the California Institute of Technology in 1961.
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