Lab 9: Regulation of lactose metabolism
... site, the other is specific for binding galactoside molecules (lactose molecules and other galactosides that are analogs of lactose). As long as there is no lactose in the cell media, the repressor protein remains bound to the DNA at the operator. However, when lactose is present, lactose binds the ...
... site, the other is specific for binding galactoside molecules (lactose molecules and other galactosides that are analogs of lactose). As long as there is no lactose in the cell media, the repressor protein remains bound to the DNA at the operator. However, when lactose is present, lactose binds the ...
PDF
... cleavage of the pre-mRNA, followed by synthesis of a polyadenylate tail onto the upstream cleavage product. Polyadenylation influences many aspects of mRNA metabolism: transcription termination by RNAP II, mRNA stability, mRNA export to the cytoplasm and the efficiency of translation are all depende ...
... cleavage of the pre-mRNA, followed by synthesis of a polyadenylate tail onto the upstream cleavage product. Polyadenylation influences many aspects of mRNA metabolism: transcription termination by RNAP II, mRNA stability, mRNA export to the cytoplasm and the efficiency of translation are all depende ...
Dynamic Model of the Process of Protein Synthesis in Eukaryotic Cells
... leaves the 5’ terminal cap and moves along the 5’ Untranslated Region (UTR) until the AUG codon is encountered. This UTR might be unstructured in which sc migrates along easily without encountering hinders or energy barriers. The 5’ UTR might however contain barriers, for example secondary structure ...
... leaves the 5’ terminal cap and moves along the 5’ Untranslated Region (UTR) until the AUG codon is encountered. This UTR might be unstructured in which sc migrates along easily without encountering hinders or energy barriers. The 5’ UTR might however contain barriers, for example secondary structure ...
Decoding the message_2 - Molecular-Biology-Resource
... Review base pairing in DNA (A with T and G with C) vs. RNA (A with U and G with C) This activity can also be done backwards (from translation to transcription); students will learn how to determine the DNA sequences for a polypeptide/protein This activity drastically simplifies how protein synthesis ...
... Review base pairing in DNA (A with T and G with C) vs. RNA (A with U and G with C) This activity can also be done backwards (from translation to transcription); students will learn how to determine the DNA sequences for a polypeptide/protein This activity drastically simplifies how protein synthesis ...
Sequence Alignment - Bilkent University
... Schematic representation of the multiple alignment of the mRNAs of a microsomal glutathione transferase homolog gene with the genomic sequence. Three GenBank mRNAs (blue) align to the same genomic locus on chromosome 9, NT_008541 (red). Three ESTs that map to this locus are presented (purple), 38 ot ...
... Schematic representation of the multiple alignment of the mRNAs of a microsomal glutathione transferase homolog gene with the genomic sequence. Three GenBank mRNAs (blue) align to the same genomic locus on chromosome 9, NT_008541 (red). Three ESTs that map to this locus are presented (purple), 38 ot ...
RNA Biology: Structures to the people! | eLife
... he structures of molecules often hold the key to understanding their roles in cells. Thus, when Watson and Crick proposed the double-helix structure for DNA, they immediately speculated on how DNA may replicate. Unfortunately, working out the structures of RNA molecules is challenging, and the techn ...
... he structures of molecules often hold the key to understanding their roles in cells. Thus, when Watson and Crick proposed the double-helix structure for DNA, they immediately speculated on how DNA may replicate. Unfortunately, working out the structures of RNA molecules is challenging, and the techn ...
Site-Directed Mutagenesis of the Proposed Catalytic Amino Acids
... cleavage releases the capsid protein from the nascent polyprotein (1, 23). The remaining cleavages occur during insertion of the protein into the endoplasmic reticulum, subsequent processing, and transport to the cell membrane (3, 9) and are postulated to be effected by cellular proteases active in ...
... cleavage releases the capsid protein from the nascent polyprotein (1, 23). The remaining cleavages occur during insertion of the protein into the endoplasmic reticulum, subsequent processing, and transport to the cell membrane (3, 9) and are postulated to be effected by cellular proteases active in ...
NON-CANONICAL TRANSCRIPTION INITIATION: THE EXPANDING
... is the moment when it contacts the 3.2 region of sigma subunits of RNAP. The 3.2 loop of sigma 70 (Tth residues 321 to 327) physically occupies the path of the elongating RNA and must be displaced to form a 5bp POST-translocated RNA:DNA hybrid (Bae et al., 2015).The 5’triphosphate of the 5nt-long PR ...
... is the moment when it contacts the 3.2 region of sigma subunits of RNAP. The 3.2 loop of sigma 70 (Tth residues 321 to 327) physically occupies the path of the elongating RNA and must be displaced to form a 5bp POST-translocated RNA:DNA hybrid (Bae et al., 2015).The 5’triphosphate of the 5nt-long PR ...
Chapter 11 Lecture PowerPoint - McGraw Hill Higher Education
... and can activate the intact promoter, or the core element alone, and it mediates the activation by the UPE ...
... and can activate the intact promoter, or the core element alone, and it mediates the activation by the UPE ...
Transcription Networks
... expression programs. This network map establishes a high connectivity between gene expression programs and cellular functions through networks of transcriptional regulatory molecules which in turn regulate other molecular players in transcription. ...
... expression programs. This network map establishes a high connectivity between gene expression programs and cellular functions through networks of transcriptional regulatory molecules which in turn regulate other molecular players in transcription. ...
10 Day Lesson Plan - Joseph L. Anderson
... DNA. There is a slight difference in the sugar phosphate structure. RNA has one base that is different than DNA. This base replaces thymine and is called uracil. RNA also comes in two different types, messenger and transfer. Messenger RNA is made in the cell nucleus as a polymerase unwinds and copie ...
... DNA. There is a slight difference in the sugar phosphate structure. RNA has one base that is different than DNA. This base replaces thymine and is called uracil. RNA also comes in two different types, messenger and transfer. Messenger RNA is made in the cell nucleus as a polymerase unwinds and copie ...
Descriptions of translation related genes that
... Small cytosolic ribosomal protein, involved in translation initiation and metabolic processes Component of heteroheptameric complexes, involved in mRNA processing Ribonucleoprotein involved in regulating mRNA translation, transport, processing PUF protein family member, involved in DNA metabolism an ...
... Small cytosolic ribosomal protein, involved in translation initiation and metabolic processes Component of heteroheptameric complexes, involved in mRNA processing Ribonucleoprotein involved in regulating mRNA translation, transport, processing PUF protein family member, involved in DNA metabolism an ...
molecular biology
... Transcription detail http://wwwclass.unl.edu/biochem/gp2/m_biology/an imation/m_animations/gene2.swf ...
... Transcription detail http://wwwclass.unl.edu/biochem/gp2/m_biology/an imation/m_animations/gene2.swf ...
PDF
... During development, translational control of mRNAs regulates gene expression. Translational control is usually achieved through binding of trans-acting factors to mRNA untranslated regions but, on p. 589, Mary Lou King and coworkers reveal a novel, structure-based mechanism for translational repress ...
... During development, translational control of mRNAs regulates gene expression. Translational control is usually achieved through binding of trans-acting factors to mRNA untranslated regions but, on p. 589, Mary Lou King and coworkers reveal a novel, structure-based mechanism for translational repress ...
PDF
... During development, translational control of mRNAs regulates gene expression. Translational control is usually achieved through binding of trans-acting factors to mRNA untranslated regions but, on p. 589, Mary Lou King and coworkers reveal a novel, structure-based mechanism for translational repress ...
... During development, translational control of mRNAs regulates gene expression. Translational control is usually achieved through binding of trans-acting factors to mRNA untranslated regions but, on p. 589, Mary Lou King and coworkers reveal a novel, structure-based mechanism for translational repress ...
10858_2015_9967_MOESM1_ESM
... S1). Transcriptions performed in the absence of DMSO clearly show heterogeneous product formation with the main products being significantly larger than the desired RNA. Incorporation of a G (35 nt. transcript) and C (39 nt. transcript) considerably reduces the transcription of additional nucleotide ...
... S1). Transcriptions performed in the absence of DMSO clearly show heterogeneous product formation with the main products being significantly larger than the desired RNA. Incorporation of a G (35 nt. transcript) and C (39 nt. transcript) considerably reduces the transcription of additional nucleotide ...
A mRNA localized to the vegetal cortex of Xenopus
... Pasceri, 1989; Yuge et al., 1990). Embryological experiments have also mapped the specification of endoderm (Wylie et al., 1987), the germ cell lineage (Whitington and Dixon, 1975) and mesoderm (Nieuwkoop, 1973) to the vegetal hemisphere. Of all these processes, only mesoderm induction has been char ...
... Pasceri, 1989; Yuge et al., 1990). Embryological experiments have also mapped the specification of endoderm (Wylie et al., 1987), the germ cell lineage (Whitington and Dixon, 1975) and mesoderm (Nieuwkoop, 1973) to the vegetal hemisphere. Of all these processes, only mesoderm induction has been char ...
Chapter_10_HB_Molecular_Biology
... 10.7 Genetic information written in codons is translated into amino acid sequences • Genetic information flows from DNA RNA protein • Nucleotide monomers represent letters in an alphabet that can form words in a language ...
... 10.7 Genetic information written in codons is translated into amino acid sequences • Genetic information flows from DNA RNA protein • Nucleotide monomers represent letters in an alphabet that can form words in a language ...
Reverse transcription-pcr (rt-pcr)
... The reaction tube of RT-PCR contains the following: Reverse transcriptase: the enzyme catalyses the reverse transcription reaction. There are several commercially available enzymes usually isolated from retroviruses. Buffer.! dNTPs (like those used in PCR), and they will be incorporated into the ...
... The reaction tube of RT-PCR contains the following: Reverse transcriptase: the enzyme catalyses the reverse transcription reaction. There are several commercially available enzymes usually isolated from retroviruses. Buffer.! dNTPs (like those used in PCR), and they will be incorporated into the ...
Eukaryotic Initiation
... • Reading frame and elongation – Most destructive mutations are frameshift mutations – Some mRNAs have recoding signal • Causes the ribosome to change its reading frame • shift to –1 frame or to +1 frame • common in viral mRNA’s ...
... • Reading frame and elongation – Most destructive mutations are frameshift mutations – Some mRNAs have recoding signal • Causes the ribosome to change its reading frame • shift to –1 frame or to +1 frame • common in viral mRNA’s ...
Having it both ways: transcription factors that bind DNA and RNA
... Besides illuminating the intricacy of biological systems, the existence of transcription factors that can bind both DNA and RNA raises at least three intriguing issues. The ®rst issue is structural. How does a single polypeptide chain make sequence-speci®c contacts with two nucleic acids whose secon ...
... Besides illuminating the intricacy of biological systems, the existence of transcription factors that can bind both DNA and RNA raises at least three intriguing issues. The ®rst issue is structural. How does a single polypeptide chain make sequence-speci®c contacts with two nucleic acids whose secon ...
Slide 1
... D, which is an exonuclease, trims seven nucleotides from this new 3′ end and then pauses while ribonuclease P makes a cut at the start of the cloverleaf, forming the 5′ end of the mature mRNA. Ribonuclease D then removes two more nucleotides, creating the 3′ end of the mature molecule. With this tRN ...
... D, which is an exonuclease, trims seven nucleotides from this new 3′ end and then pauses while ribonuclease P makes a cut at the start of the cloverleaf, forming the 5′ end of the mature mRNA. Ribonuclease D then removes two more nucleotides, creating the 3′ end of the mature molecule. With this tRN ...
Document
... • As researchers learned more about proteins – The made minor revision to the one gene–one ...
... • As researchers learned more about proteins – The made minor revision to the one gene–one ...
MS Word document - Sequence Ontology
... transcripts. There is a time difference between primary and processed transcripts, that cannot be captured by either isa or part_of relationships. For example, in the current model, cap is part_of mRNA is part_of processed_transcript is part_of transcript is part_of gene. BUT cap is not part of a ge ...
... transcripts. There is a time difference between primary and processed transcripts, that cannot be captured by either isa or part_of relationships. For example, in the current model, cap is part_of mRNA is part_of processed_transcript is part_of transcript is part_of gene. BUT cap is not part of a ge ...
Messenger RNA
Messenger RNA (mRNA) is a large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence of the protein products of gene expression. Following transcription of primary transcript mRNA (known as pre-mRNA) by RNA polymerase, processed, mature mRNA is translated into a polymer of amino acids: a protein, as summarized in the central dogma of molecular biology.As in DNA, mRNA genetic information is in the sequence of nucleotides, which are arranged into codons consisting of three bases each. Each codon encodes for a specific amino acid, except the stop codons, which terminate protein synthesis. This process of translation of codons into amino acids requires two other types of RNA: Transfer RNA (tRNA), that mediates recognition of the codon and provides the corresponding amino acid, and ribosomal RNA (rRNA), that is the central component of the ribosome's protein-manufacturing machinery.The existence of mRNA was first suggested by Jacques Monod and François Jacob, and subsequently discovered by Jacob, Sydney Brenner and Matthew Meselson at the California Institute of Technology in 1961.