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4. Protein Synthesis and Biotechnology
4. Protein Synthesis and Biotechnology

... subunits: a five-carbon pentose sugar, a phosphoric acid group, and one of four nitrogen bases. (For DNA these nitrogen bases are adenine, guanine, cytosine, or thymine.) DNA and RNA differ in a number of major ways. A DNA nucleotide contains a deoxyribose sugar, but RNA contains ribose sugar. The n ...
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... Secondary (2o) structure is a regular repeating structure due to folding of the polypeptide chain. The main types are alpha-helix and beta sheet (either parallel or anti-parallel). Secondary structure is maintained by hydrogen bonds formed between a hydrogen (donor) attached to the nitrogen in the b ...
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... • Translational regulatory proteins – recognize sequences in mRNA and inhibit translation (sometimes at the start codon) • Antisense RNA – a RNA strand that is complementary to mRNA binds to the mRNA and keeps it from being translated ...
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... Eukaryotic translation initiation sequences from sequence being amplified. Increases efficiency of translation initiation. • 6–10 bases upstream of promoter. Improves efficiency of promoter. • 3- to 6-base spacer between promoter sequence and Kozak sequence. Ensures transcription starts ...
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... The control of gene expression by transcription activation and repression has been studied extensively in bacteria. As an example, the E. coli lac operon, which encodes 3 genes (lacZYA) involved in lactose metabolism, uses both mechanisms of control (Fig. 7.3). A specific repressor protein (the lac ...
Observations and Analysis of Snork DNA
Observations and Analysis of Snork DNA

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... By recreating certain conditions thought to exist on primitive Earth 3.7 billion years ago, the UCSD researchers produced a chemical reaction between uracil--one of four nucleic acid bases found in RNA--and formaldehyde, a simple molecule thought to have been abundant on prebiotic Earth. The result ...
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... once it finds a double-stranded RNA molecule, cuts it up with an endonuclease (Dicer), separates the two strands, and then proceeds to destroy other single-stranded RNA molecules that are complementary to one of those sequences. dsRNAs direct the creation of small interfering RNAs (siRNAs) which tar ...
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... pathway, 1 DNA damage is an intracellular signal that is passed via 2 protein kinases and leads to activation of 3 p53. Activated p53 promotes transcription of the gene for a protein that inhibits the cell cycle. The resulting suppression of cell division ensures that the damaged DNA is not replicat ...
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... specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP10 gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. ...
DNA Replication and Protein Synthesis
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... them from one another.  Base pairing occurs between incoming RNA nucleotides and the DNA nucleotides of the gene (template) • recall RNA uses uracil instead of thymine ...
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... TRANSLATION/PROTEIN SYNTHESIS 30. What is produced in translation? PROTEIN OR POLYPEPTIDE 31. Ribosomes are made up of rRNA , 2 PARTS . Know the steps of translation: 32. Identify the following structures in the diagram: A: DNA TEMPLATE B: DNA COMPLIMENTARY STRAND C: NUCLEOTIDE D: mRNA ...
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Chapter 17 lecture notes
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... Many eukaryotic genes code for a set of closely related polypeptides in a process called alternative splicing. ...
The Transcription Process
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... formed during transcription, thereby releasing the newly synthesized RNA. In eukaryotes, termination of transcription occurs by different processes, depending upon the exact polymerase utilized. For pol I genes, transcription is stopped using a termination factor, through a mechanism similar to rho- ...
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... protein genes, have 5’ mG caps and most often have poly-A tails. A few exceptions to this paradigm were known (for example, ribosomal RNA and transfer RNA, which are ...
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chapter 17 from gene to protein

... began in the early 1960s.  Marshall Nirenberg determined the first match: UUU coded for the amino acid phenylalanine.  He created an artificial mRNA molecule entirely of uracil and added it to a test tube mixture of amino acids, ribosomes, and other components for protein synthesis.  This “poly-U ...
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Targeting the GAA-Repeat Region with Oligonucleotides for the

... expansion of GAA/TTC triplet repeats in the first intron of FXN gene. Repeat expansion beyond a certain threshold causes transcriptional defects which reduce FXN mRNA and protein levels. Despite long-standing research in the pathogenesis of FRDA, the means by which GAA-repeat number elevation leads ...
Biological information flow
Biological information flow

... different patterns, generating proteins with different functions. ...
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Messenger RNA



Messenger RNA (mRNA) is a large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence of the protein products of gene expression. Following transcription of primary transcript mRNA (known as pre-mRNA) by RNA polymerase, processed, mature mRNA is translated into a polymer of amino acids: a protein, as summarized in the central dogma of molecular biology.As in DNA, mRNA genetic information is in the sequence of nucleotides, which are arranged into codons consisting of three bases each. Each codon encodes for a specific amino acid, except the stop codons, which terminate protein synthesis. This process of translation of codons into amino acids requires two other types of RNA: Transfer RNA (tRNA), that mediates recognition of the codon and provides the corresponding amino acid, and ribosomal RNA (rRNA), that is the central component of the ribosome's protein-manufacturing machinery.The existence of mRNA was first suggested by Jacques Monod and François Jacob, and subsequently discovered by Jacob, Sydney Brenner and Matthew Meselson at the California Institute of Technology in 1961.
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