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Slide 1
Slide 1

... The publicly available microarray dataset from the Hedenfalk et al. (2001) breast cancer study consists of gene-expression data for three classes of tumour, BCRA1related cancer, BCRA2-related cancer, and sporadic cases of breast cancer. ...
Διαφάνεια 1
Διαφάνεια 1

... • Investigations revealed that certain miRNAs (epimiRNAs) themselves counteract CpG methylation. • Regulate the components of epigenetic machinery, creating a tightly controlled feedback mechanism. • Histone modification is another epigenetic mechanism that can affect miRNA expression as shown in br ...
We describe a method for the formation of hybrid
We describe a method for the formation of hybrid

... HEp2 (CCL23) cells. Thirteen recombinants lacked the unique EcoRI site located downstream of the IFN-al coding sequence and probably contained entirely a2-specific coding sequences; they were not analyzed further. Forty-four of the remaining plasmids were sequenced between the upstream Bglll site an ...
Exam1 2012 Life Student
Exam1 2012 Life Student

... ____ 72. What process occurs in both the aerobic and anaerobic pathways? a. glycolysis b. Krebs cycle c. electron transport chain d. fermentation e. anaerobic processes ____ 73. The role of oxygen in cellular respiration is to a. combine with carbon atoms to form carbon dioxide. b. allow glycolysis ...
(HOM) genes. Antennapedia and Bithorax Complexes (WR
(HOM) genes. Antennapedia and Bithorax Complexes (WR

... there was a gradient of a repressor molecule, highest in T2 (where no BX-C genes are expressed) and lowest in A8 (where all are expressed). Further, he postulated that the promoter region of each gene in the BX-C had a different affinity for the repressor, with iab8 having the highest affinity (and ...
Lab Practicum #2
Lab Practicum #2

... 5. What happens in conjugation? Know possible conjugation results for the following matings: F+ x F-, Hfr x F-. Given locations (F-plasmid versus chromosome) and types of antibiotic resistance genes (AmpR, StrR, NalR) for different E. coli strains, be able to predict which will grow on different ant ...
Bb - gpisd
Bb - gpisd

... ________mutations – allows organism to ____________ ______: provides _______________ __________ mutations – ________ harmful nor helpful to organism ...
What is the correct term for twins that are born attached together?
What is the correct term for twins that are born attached together?

... ________ A family record that shows how a trait is inherited over several generations is called a _____________ A. karyotype B. Punnett square C. pedigree D. periodic table ________ A person that has one copy of a recessive autosomal allele and does not express the trait but can pass it on to his/h ...
Topic 7.1 Replication and DNA Structure
Topic 7.1 Replication and DNA Structure

... 7.1 DNA Structure & Replication Essential Idea: The structure of DNA is ideally suited to its function. DNA is a double helix, consisting of two anti-parallel chains of polynucleotides that are held together by hydrogen bonds between complementary bases on the different strands. This structure allow ...
SADDLEBACK COLLEGE BIOLOGY 20 EXAMINATION 3 STUDY
SADDLEBACK COLLEGE BIOLOGY 20 EXAMINATION 3 STUDY

... 5. Discuss the 5 control factors of cell division and briefly why cancer cells are easier to grow in the lab than other cells. 6. Briefly discuss how horizontal gene transfer can increase genetic diversity in asexually reproducing prokaryotes. 7. Compare and contrast PCR and RFLP. When would one be ...
Physiological Homeostasis means …………
Physiological Homeostasis means …………

... Base is added in, will shift the reading frame and all amino acids ...
Chapter 7 Note taking Form
Chapter 7 Note taking Form

...  An organism's ___________________________ represents the two alleles inherited for a given trait such as CC or cc. For an organism to be a ____________________, the genotype must include one copy of a recessive allele (Ex. Bb). Carriers ____________________ exhibit the physical trait, but have a _ ...
microsatellite marker analysis in the treatment and diagnosis of
microsatellite marker analysis in the treatment and diagnosis of

... contrast to family B, in which all affected persons died before 30 years of age. In family A, the founder of the mutation was the patient I-2. The affected children III-10 and III-12 received an associated with the disease haplotype 5-2-3 from their affected mothers (II-5 and II-7). The other childr ...
Biol120 Mock Final Examination (v2.0)
Biol120 Mock Final Examination (v2.0)

... Important note: This mock final exam was written by your Biology 120 Peer Mentors, not your professors! It is designed to help you test yourself on topics and concepts covered in class but should not be considered an exact preview of the final exam. 1. The plant cell wall is made of: a) Chitin b) Ce ...
Suracell: My Test Results
Suracell: My Test Results

... How do we know the efficiency of your genes in each category? Our DNA contains approximately 30,000 genes. Within our cells, our DNA is organized into 23 pairs of chromosomes. Genetic variations in our DNA are called SNPs (Single Nucleotide Polymorphisms). If we compare the DNA of two individuals, w ...
The_RAY_Manual
The_RAY_Manual

... E. coli as well as ES-cells, permitting a selection for the recombination product in E.coli. Cotransformed yeast colonies are pooled, extrachromosomal DNA is prepared and electroporated into E. coli. Bacterial transformants containing the recombination product are selected on plates containing kana ...
RECOMBINANT DNA USING BACTERIAL PLASMIDS
RECOMBINANT DNA USING BACTERIAL PLASMIDS

... RECOMBINANT DNA USING BACTERIAL PLASMIDS BACKGROUND: Bacteria have not only their normal DNA, they also have a circular DNA called a plasmid. It is a wonderful ally for biologists who desire to get bacteria to produce very specific proteins. The plasmids conveniently can be cut, fused with other DNA ...
chapter 27 - applied genetics
chapter 27 - applied genetics

... CYSTIC FIBROSIS SICKLE-CELL DISEASE PHENYLKETONURIA (PKU) HUNTINGTON DISEASE DOWN’S SYNDROME ...
CH 16 and 17 PowerPoint
CH 16 and 17 PowerPoint

... By the Numbers • The human genome contains 3 billion chemical nucleotide bases (A, C, T, and G). • The average gene consists of 3000 bases, but sizes vary greatly, with the largest known human gene being dystrophin at 2.4 million bases. ...
Gene Section USP7 (ubiquitin specific peptidase 7 (herpes virus- associated))
Gene Section USP7 (ubiquitin specific peptidase 7 (herpes virus- associated))

... acids, comprising three conserved domain architectures Finger, Palm, and Thumb. It has highly conserved Cys, Asp(I), His, and Asn/Asp(II) domains, which are responsible for deubiquitination activities. ...
Restriction Enzyme Digestion
Restriction Enzyme Digestion

... 2= Restriction enzyme activity is measured in “units.” One unit is defined as the amount of the enzyme required to digest 1 ug of DNA in 60 minutes. 10-fold overdigestion is recommended. In our lab, use 10 units of enzyme for DNA amounts of 1 ug or less. Add 10 units for each additional 0.1-1 ug of ...
File
File

... A zygote with twice the number of chromosomes as a gamete An egg with half the number of chromosomes as a zygote A gamete with twice the number of chromosomes as a zygote A zygote with half the number of chromosomes as a gamete ...
Gene splicing
Gene splicing

... RNAs bound by additional small proteins.  The small RNAs which make up the spliceosome are small nuclear RNAs (snRNA's).  The snRNAs combine with proteins to comprise, ...
Genetic Disorders
Genetic Disorders

... • Heterozygotes, representing 1:500, have 2-3x elevation of cholesterol levels with xanthomas and premature atherosclerosis • Homozygotes develop extensive xanthomas, as well as coronary, cerebral and peripheral vascular disease at an early age, and may develop MI before the age of 20 ...
PPT lecture slides
PPT lecture slides

... development of human obesity in an age-dependent fashion The 677C-->T mutation of MTHFR was present in 26.1% of adults with idiopathic osteonecrosis of the femur head and appears to have a role in the complex pathophysiology of the disease. Genomic deletion of DLC-1 was observed in 40% of breast tum ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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