gene families
... least 30,000 and maybe 40,000 genes, apparently because of the complex aquatic environments it inhabits (several “species” in one genome!). The Ixodes scapularis deer tick (vector of Lyme disease) genome is huge at around 2.2 Gbp, and the Rhodnius prolixus kissing bug (vector of Chagas disease) is a ...
... least 30,000 and maybe 40,000 genes, apparently because of the complex aquatic environments it inhabits (several “species” in one genome!). The Ixodes scapularis deer tick (vector of Lyme disease) genome is huge at around 2.2 Gbp, and the Rhodnius prolixus kissing bug (vector of Chagas disease) is a ...
Microarray data calculations For study 1, the genes
... regression, similar to the microarray analysis as described above, with the difference of having the sample concentration, the test day, the perfusion procedure, repeats, and the best housekeeping gene among 18S ribosomal RNA, GAPDH and calnexin included. For each gene, the treatment was then accoun ...
... regression, similar to the microarray analysis as described above, with the difference of having the sample concentration, the test day, the perfusion procedure, repeats, and the best housekeeping gene among 18S ribosomal RNA, GAPDH and calnexin included. For each gene, the treatment was then accoun ...
Overview of Recombinant DNA Experiments Covered by
... 1) Those that can neither replicate nor generate nucleic acids that can replicate in any living cell (e.g. oligonucleotides or other synthetic that do not contain an origin of replication or contain elements known to interact with either DNA or RNA polymerase), and 2) Those that are not designed to ...
... 1) Those that can neither replicate nor generate nucleic acids that can replicate in any living cell (e.g. oligonucleotides or other synthetic that do not contain an origin of replication or contain elements known to interact with either DNA or RNA polymerase), and 2) Those that are not designed to ...
excercise handout
... 1. Provide findings for 8 genes as best you can. For at least 3 genes, all information should be fully completed based on having read 1+ article as thoroughly as you can. For the other 5 genes, the required information should be filled out as best you can. If you have more than 8 genes, you will get ...
... 1. Provide findings for 8 genes as best you can. For at least 3 genes, all information should be fully completed based on having read 1+ article as thoroughly as you can. For the other 5 genes, the required information should be filled out as best you can. If you have more than 8 genes, you will get ...
et al. MATERIALS AND METHODS Construction of the synthetic network
... The numerical prefactors in all terms come from time averaging due to the finite protein life-time. It is assumed that the effective protein and plasmid life-times are dominated by dilution due to cell growth and therefore are determined by the cell doubling time. The coefficients presented here cor ...
... The numerical prefactors in all terms come from time averaging due to the finite protein life-time. It is assumed that the effective protein and plasmid life-times are dominated by dilution due to cell growth and therefore are determined by the cell doubling time. The coefficients presented here cor ...
Studies on Chlamydomonas Chloroplast Transformation: Foreign
... Recently, Sanford and co-workers (Klein et al., 1987) have developed a DNA transfer technique that relies upon bombardment of recipient cells with high-velocity tungsten microprojectiles coated with DNA. Using this delivery system, Klein et al. (1988) have measured transient expression of the chlora ...
... Recently, Sanford and co-workers (Klein et al., 1987) have developed a DNA transfer technique that relies upon bombardment of recipient cells with high-velocity tungsten microprojectiles coated with DNA. Using this delivery system, Klein et al. (1988) have measured transient expression of the chlora ...
Mutations, Karyotyping, Pedigrees
... What is a pedigree? •similar to a family tree •Shows genotypes and phenotypes of a genetic trait through several generations of a family ...
... What is a pedigree? •similar to a family tree •Shows genotypes and phenotypes of a genetic trait through several generations of a family ...
Document
... • Very similar to the Chg. Corr., but it only considers positive changes. All negative values for the arc tangent are set to zero. • Make a new vector A from a by looking at the change b/w each pair of elements of a. • The value created b/w two values a i and a i+1 is max(atan(a i+1 /a i )- /4.0). ...
... • Very similar to the Chg. Corr., but it only considers positive changes. All negative values for the arc tangent are set to zero. • Make a new vector A from a by looking at the change b/w each pair of elements of a. • The value created b/w two values a i and a i+1 is max(atan(a i+1 /a i )- /4.0). ...
Genetics Powerpoint
... • Example: In rabbits black coat (B) is dominant over brown (b) and straight hair (H) is dominant to curly (h). Cross a rabbit that is homozygous dominant for both traits with a rabbit that is homozygous dominant for black coat and heterozygous for straight hair. Then give the phenotypic ratio for ...
... • Example: In rabbits black coat (B) is dominant over brown (b) and straight hair (H) is dominant to curly (h). Cross a rabbit that is homozygous dominant for both traits with a rabbit that is homozygous dominant for black coat and heterozygous for straight hair. Then give the phenotypic ratio for ...
Dosage Compensation: Transcription-Level Regulation of X
... Drosophila, it is not surprising that gene activity of X and autosome segments is autonomous in translocations: X-linked genes are subjected to dosage compensation even when they are relocated elsewhere in the genome; autosomal genes transposed to the X do not become dosage compensated. An example o ...
... Drosophila, it is not surprising that gene activity of X and autosome segments is autonomous in translocations: X-linked genes are subjected to dosage compensation even when they are relocated elsewhere in the genome; autosomal genes transposed to the X do not become dosage compensated. An example o ...
Operon: Lac trp Is the metabolic pathway anabolic or catabolic
... 1. Under what circumstances would the lac operon be “on” versus “off”? The trp operon? The lac operon would be off when there is no lactose in the cell. The lac operon would be on when lactose is present and there is little or no glucose in the cell. However, the lac operon would be off (or operatin ...
... 1. Under what circumstances would the lac operon be “on” versus “off”? The trp operon? The lac operon would be off when there is no lactose in the cell. The lac operon would be on when lactose is present and there is little or no glucose in the cell. However, the lac operon would be off (or operatin ...
Reproduction—The Importance of Reproduction
... • If you could look at DNA in detail, you would see that it is shaped like a twisted ladder. • The two sides of the ladder form the backbone of the DNA molecule. • The sides support the rungs, which hold all the genetic information. ...
... • If you could look at DNA in detail, you would see that it is shaped like a twisted ladder. • The two sides of the ladder form the backbone of the DNA molecule. • The sides support the rungs, which hold all the genetic information. ...
Binary Vectors
... • synthetic T-DNA borders and a bar gene, which confers the plants resistance to the herbicide phosphinothricin. Also, the vectors use the phage P1 Cre/loxP site-specific recombinase system, which permits the transfer and integration of a target and marker genes as a single T-DNA unit into the plant ...
... • synthetic T-DNA borders and a bar gene, which confers the plants resistance to the herbicide phosphinothricin. Also, the vectors use the phage P1 Cre/loxP site-specific recombinase system, which permits the transfer and integration of a target and marker genes as a single T-DNA unit into the plant ...
Gene Section EXT1 (exostoses (multiple) 1) Atlas of Genetics and Cytogenetics
... proteoglycan biosynthesis controlling diffusion of an important segment polarity protein called Hedgehog (Hh). ...
... proteoglycan biosynthesis controlling diffusion of an important segment polarity protein called Hedgehog (Hh). ...
... Consider first the naming of a new N. crassa gene when its ortholog in another species is known, and when there are experimental data supporting its function in N. crassa that are consistent with the function of the known ortholog. As an example, consider the recently identified N. crassa gene spo11 ...
Gene Section PRDM1 (PR domain containing 1, with ZNF domain)
... methyltransferase G9a (Gyory et al., 2004), histone deacetylases HDAC1 and HDAC2 (Yu et al., 2000), and H3 lysine demethylase LSD1 (Su et al., 2009). PRDM1 can also tether Groucho family of transcription factors to mediate repression of gene transcription (Ren et al., 1999). Interaction of PRDM1/Bli ...
... methyltransferase G9a (Gyory et al., 2004), histone deacetylases HDAC1 and HDAC2 (Yu et al., 2000), and H3 lysine demethylase LSD1 (Su et al., 2009). PRDM1 can also tether Groucho family of transcription factors to mediate repression of gene transcription (Ren et al., 1999). Interaction of PRDM1/Bli ...
Studying DNA replication to find smarter cancer drugs
... for selective killing of cancer cells. Before a cell can successfully divide to produce two daughter cells, it must precisely duplicate its chromosomal DNA. This process, DNA replication, is targeted by several ‘antimetabolite’ cancer drugs such as methotrexate and fluorouracil that reduce the suppl ...
... for selective killing of cancer cells. Before a cell can successfully divide to produce two daughter cells, it must precisely duplicate its chromosomal DNA. This process, DNA replication, is targeted by several ‘antimetabolite’ cancer drugs such as methotrexate and fluorouracil that reduce the suppl ...
Weldon_McVean - Wellcome Trust Centre for Human Genetics
... • We know from previous work in a few regions that hotspot locations tend not to be shared between humans and chimpanzees • Calculations suggested that only 40% of human hotspots were driven by PRDM9 binding ...
... • We know from previous work in a few regions that hotspot locations tend not to be shared between humans and chimpanzees • Calculations suggested that only 40% of human hotspots were driven by PRDM9 binding ...
Ch09 Lecture-DNA and Its Role in Heredity
... Mutations are changes in the nucleotide sequence of DNA that are passed on from one cell, or organism, to another. Mutations occur by a variety of processes. Errors that are not corrected by repair systems are passed on to daughter cells. Mutations are of two types: ...
... Mutations are changes in the nucleotide sequence of DNA that are passed on from one cell, or organism, to another. Mutations occur by a variety of processes. Errors that are not corrected by repair systems are passed on to daughter cells. Mutations are of two types: ...
Uses and abuses of genetic engineering
... (“making transgenics”) has been with us for more than a decade. Today these earlier strategies are giving way to more sophisticated approaches, such as lineage-specific conditional knockouts in which a selected gene can be deleted in a ...
... (“making transgenics”) has been with us for more than a decade. Today these earlier strategies are giving way to more sophisticated approaches, such as lineage-specific conditional knockouts in which a selected gene can be deleted in a ...
Mcbio 316: Exam 1A Answers (10)1. A wild
... c. What frequency would you expect to find revertants that are both His+ and Cys+? ANSWER: Simply multiply the frequency of each independent event: 10-5 x 10-5 = 10-11 [Copy #1 10-12; copy #3 10-11] d. How could you directly select for such double revertants? ANSWER: Plate the cells on minimal mediu ...
... c. What frequency would you expect to find revertants that are both His+ and Cys+? ANSWER: Simply multiply the frequency of each independent event: 10-5 x 10-5 = 10-11 [Copy #1 10-12; copy #3 10-11] d. How could you directly select for such double revertants? ANSWER: Plate the cells on minimal mediu ...