Genome engineering of mammalian haploid embryonic stem cells
... Streptococcus pyogenes is the simplest. In this system, a single gene encoding the Cas9 protein and two RNAs, a mature CRISPR RNA (crRNA) and a partially complementary trans-acting RNA (tracrRNA), are sufficient for RNA-guided cleavage of foreign DNAs (Jinek et al., 2012). Maturation of crRNA requir ...
... Streptococcus pyogenes is the simplest. In this system, a single gene encoding the Cas9 protein and two RNAs, a mature CRISPR RNA (crRNA) and a partially complementary trans-acting RNA (tracrRNA), are sufficient for RNA-guided cleavage of foreign DNAs (Jinek et al., 2012). Maturation of crRNA requir ...
Independent specialization of the human and mouse X
... were averaged, it was important to rule out the possibility that only one family member was actively transcribed in the testis—which we did by scrutinizing the testis mRNA-seq data for sequence variants that differentiated members of a gene family (Supplementary Table 9). The testis-predominant expr ...
... were averaged, it was important to rule out the possibility that only one family member was actively transcribed in the testis—which we did by scrutinizing the testis mRNA-seq data for sequence variants that differentiated members of a gene family (Supplementary Table 9). The testis-predominant expr ...
Inheritance of Color And The Polled Trait
... roan. There is also incomplete dominance between the gene for red (R) and the gene for white (r), producing the red-roan when these two genes are present. An individual with two genes for white (r) will be white. Another set of genes determines if the color is diluted or not diluted. The gene for d ...
... roan. There is also incomplete dominance between the gene for red (R) and the gene for white (r), producing the red-roan when these two genes are present. An individual with two genes for white (r) will be white. Another set of genes determines if the color is diluted or not diluted. The gene for d ...
dominance relationships between two allelic genfs
... lacking glycosylation genes could easily be detected by its typical morphology of the petals. The other crossover product with both gG and gx cannot be scored in these crosses, as the presence of gene gG suppresses the phenotypic expression of g x (BREDERODE and NIGTEVECHT 197213). Among a total pro ...
... lacking glycosylation genes could easily be detected by its typical morphology of the petals. The other crossover product with both gG and gx cannot be scored in these crosses, as the presence of gene gG suppresses the phenotypic expression of g x (BREDERODE and NIGTEVECHT 197213). Among a total pro ...
CAPSTONE - Bioinformatics at School of Informatics
... • Multiple Genome Browser (MGB) ▫ synteny regions between genomes. ...
... • Multiple Genome Browser (MGB) ▫ synteny regions between genomes. ...
Non-Mendelian Genetics
... 6. Expressivity Sometimes the same genotype will produce different “degrees” of phenotype in individuals • Expressivity = the severity or extent of the phenotype an individual shows ex Hypercholesterolemia – Some individuals have extremely high cholesterol from birth, others can control with diet a ...
... 6. Expressivity Sometimes the same genotype will produce different “degrees” of phenotype in individuals • Expressivity = the severity or extent of the phenotype an individual shows ex Hypercholesterolemia – Some individuals have extremely high cholesterol from birth, others can control with diet a ...
A Novel Chimeric Low-Molecular-Weight Glutenin
... and the three subregions of the C-terminal domain, as well as eight conserved cysteine residues. The signal peptide sequences were highly conserved in the 4 cloned LMW-m genes and no dot mutation or insertion/ deletion was presented in this domain. Compared to other LMW-m type genes, their eight cys ...
... and the three subregions of the C-terminal domain, as well as eight conserved cysteine residues. The signal peptide sequences were highly conserved in the 4 cloned LMW-m genes and no dot mutation or insertion/ deletion was presented in this domain. Compared to other LMW-m type genes, their eight cys ...
Chromosome segregation: Samurai separation
... protein encoded by a gene called pituitary tumor transforming gene (PTTG). Each of these vertebrate proteins is only about 15% identical in sequence to yeast Pds1, but their genes appear to be functional homologs of PDS1 and Cut2. PTTG associates with hESP1 and nondegradable mutant forms of xPDS1 ca ...
... protein encoded by a gene called pituitary tumor transforming gene (PTTG). Each of these vertebrate proteins is only about 15% identical in sequence to yeast Pds1, but their genes appear to be functional homologs of PDS1 and Cut2. PTTG associates with hESP1 and nondegradable mutant forms of xPDS1 ca ...
Full-text PDF
... Genomes and the genetic information they contain have a generally hierarchical structure: DNA consists of two strands, each of which contains features, which in turn have an associated type and function, and so on. Furthermore, some biological reactions occurring within the genome also tend to be hi ...
... Genomes and the genetic information they contain have a generally hierarchical structure: DNA consists of two strands, each of which contains features, which in turn have an associated type and function, and so on. Furthermore, some biological reactions occurring within the genome also tend to be hi ...
msc_botnay_pre_pap1_bl2
... replication or transcription machinery to naked DNA. These modifications include ubiquitination, acetylation, methylation and phosphorylation of some specific amino acid residues of histones. Acetylation and methylation occur on the free amino groups of lysines residues. Methylation also occurs on a ...
... replication or transcription machinery to naked DNA. These modifications include ubiquitination, acetylation, methylation and phosphorylation of some specific amino acid residues of histones. Acetylation and methylation occur on the free amino groups of lysines residues. Methylation also occurs on a ...
Full Text
... in the Platyhelminthes phyla. In situ hybridization studies showed an ubiquitous expression of GtPax6A in the whole central nervous system (Fig. 1A). During head regeneration Pax6 is activated in the new cephalic ganglia (Fig. 1B). Electron microscopy in situ hybridization also showed expression in ...
... in the Platyhelminthes phyla. In situ hybridization studies showed an ubiquitous expression of GtPax6A in the whole central nervous system (Fig. 1A). During head regeneration Pax6 is activated in the new cephalic ganglia (Fig. 1B). Electron microscopy in situ hybridization also showed expression in ...
9. Axis Specification in Drosophila
... Torso kinases inactivate an inhibitor of tailless and huckebein Tailless and Huckebein specify termini Distinction between anterior and posterior = Bicoid Bicoid = acron formation ...
... Torso kinases inactivate an inhibitor of tailless and huckebein Tailless and Huckebein specify termini Distinction between anterior and posterior = Bicoid Bicoid = acron formation ...
Induction of the white egg3 mutant phenotype by injection of the
... of GFP dsRNA did not reduce endogenous Bmwh3 mRNA. Sequence-specific silencing was also shown for GFP expression by interfering with the transient expression of GFP from a plasmid without affecting the colour of the eggs and larvae. This is the first report in which it is shown that RNAi acts in the ...
... of GFP dsRNA did not reduce endogenous Bmwh3 mRNA. Sequence-specific silencing was also shown for GFP expression by interfering with the transient expression of GFP from a plasmid without affecting the colour of the eggs and larvae. This is the first report in which it is shown that RNAi acts in the ...
GHS-Express database http://genecanvas.ecgene.net/uploads/Fo
... expressions with a p-value < 10-5 are reported (n=225615) together with the position of the SNP and of the associated gene. “cistransDistance” is 109 when the SNP and gene are ...
... expressions with a p-value < 10-5 are reported (n=225615) together with the position of the SNP and of the associated gene. “cistransDistance” is 109 when the SNP and gene are ...
Using gene expression to investigate the genetic basis of complex
... these approaches (a field also called Genetical Genomics) explain variance in transcript levels of nearby genes (so called cis eQTLs) but a few distal acting regulators have also been reported (trans associations). The sample sizes of genome-wide expression association studies have been fairly small ...
... these approaches (a field also called Genetical Genomics) explain variance in transcript levels of nearby genes (so called cis eQTLs) but a few distal acting regulators have also been reported (trans associations). The sample sizes of genome-wide expression association studies have been fairly small ...
Hox genes and evolution of body plan Prof. LS Shashidhara
... ¾Changes in domain of Hox gene expression ¾ Changes in Hox gene that gives the protein new properties ¾Changes in Hox-protein responsive elements of downstream genes ...
... ¾Changes in domain of Hox gene expression ¾ Changes in Hox gene that gives the protein new properties ¾Changes in Hox-protein responsive elements of downstream genes ...
Molecular Pathology Studies of Mesothelioma in VDC-exposed F344/N Rats
... • Generates supplementary and supportive data for the NTP on molecular characterization of chemically induced rodent tumors ...
... • Generates supplementary and supportive data for the NTP on molecular characterization of chemically induced rodent tumors ...
Bio1100 Ch15W
... • Another example: Hemophilia is a __________________ trait defined by the absence of one or more clotting factors. • prolonged bleeding because a firm clot forms slowly. ...
... • Another example: Hemophilia is a __________________ trait defined by the absence of one or more clotting factors. • prolonged bleeding because a firm clot forms slowly. ...
Analysis of sequence variations in the factor VIII gene in
... f8 gene are usually asymptomatic carriers. Haemophilia A has been maintained in the population by an equilibrium between new mutations and selection. It therefore has a very high level of mutational heterogeneity and about 30% of the cases arise from new mutations (1). Haemophilia A occurs in mild, ...
... f8 gene are usually asymptomatic carriers. Haemophilia A has been maintained in the population by an equilibrium between new mutations and selection. It therefore has a very high level of mutational heterogeneity and about 30% of the cases arise from new mutations (1). Haemophilia A occurs in mild, ...
Human Sex Determination
... between mouse and human SRY protein exist, particularly at the C terminal. In the mouse, the Cterminal of Sry is 252 amino acids long (in humans, it is only 69 amino acids) and contains a glutamine/ histidine-rich domain that mediates transactivation in vitro. No such region has been identified in h ...
... between mouse and human SRY protein exist, particularly at the C terminal. In the mouse, the Cterminal of Sry is 252 amino acids long (in humans, it is only 69 amino acids) and contains a glutamine/ histidine-rich domain that mediates transactivation in vitro. No such region has been identified in h ...
Angelman Syndrome (AS) and UBE3A (E6-AP)
... Monoallelic expression of an isoform found in mouse brain tissue depression of expression is also evident in the hippocampus of a paternal UPD mouse Evidence for decrease of expression in human brain Clinical description of AS indicates a developmental brain disorder and so differential expression o ...
... Monoallelic expression of an isoform found in mouse brain tissue depression of expression is also evident in the hippocampus of a paternal UPD mouse Evidence for decrease of expression in human brain Clinical description of AS indicates a developmental brain disorder and so differential expression o ...
Spatially ordered transcription of regulatory DNA in
... change as development proceeds (Fig. 4). Probes spanning the whole iab region (from +58 to +150 kb) hybridize strongly to transcripts in PS13-15. When the germ band is extended both ectoderm and mesoderm show expression (Fig. 4C,E). Later, the signal is particularly strong in the ventral nerve cord, ...
... change as development proceeds (Fig. 4). Probes spanning the whole iab region (from +58 to +150 kb) hybridize strongly to transcripts in PS13-15. When the germ band is extended both ectoderm and mesoderm show expression (Fig. 4C,E). Later, the signal is particularly strong in the ventral nerve cord, ...