Specific PCR Primers for the Identification of Salmonella enterica
... Total of 285 chicken-related samples either obtained directly from markets or collected as swabs from chicken farms were detected for S. enterica serovar Enteritidis using both the conventional culture method (BAM) and the PCR procedure developed in this study. Table 3 shows that a single 535-bp PCR ...
... Total of 285 chicken-related samples either obtained directly from markets or collected as swabs from chicken farms were detected for S. enterica serovar Enteritidis using both the conventional culture method (BAM) and the PCR procedure developed in this study. Table 3 shows that a single 535-bp PCR ...
PDF
... known about the genetic control of patterned cell differentiation during organ development. We show that the conserved Teashirt transcription factors are decisive for the differentiation of a subset of secretory cells, stellate cells, in Drosophila melanogaster renal tubules. Teashirt controls the e ...
... known about the genetic control of patterned cell differentiation during organ development. We show that the conserved Teashirt transcription factors are decisive for the differentiation of a subset of secretory cells, stellate cells, in Drosophila melanogaster renal tubules. Teashirt controls the e ...
Mak5 and Ebp2 Act Together on Early Pre-60S Particles and
... Ribosomes are the molecular machines that translate mRNAs into proteins. The synthesis of ribosomes is therefore a fundamental cellular process and consists in the ordered assembly of 79 ribosomal proteins (r-proteins) and four ribosomal RNAs (rRNAs) into a small 40S and a large 60S ribosomal subuni ...
... Ribosomes are the molecular machines that translate mRNAs into proteins. The synthesis of ribosomes is therefore a fundamental cellular process and consists in the ordered assembly of 79 ribosomal proteins (r-proteins) and four ribosomal RNAs (rRNAs) into a small 40S and a large 60S ribosomal subuni ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... Of particular interest were studies on some proteins that bound to nucleic acids (Diggle and Peacocke 1968; Haydon and Peacocke 1968; Diggle and Peacocke 1971; Diggle, McVittie et al. 1975). Now it is known that hundreds perhaps thousands of proteins in the body bind to nucleic acids. In Peacocke’s ...
... Of particular interest were studies on some proteins that bound to nucleic acids (Diggle and Peacocke 1968; Haydon and Peacocke 1968; Diggle and Peacocke 1971; Diggle, McVittie et al. 1975). Now it is known that hundreds perhaps thousands of proteins in the body bind to nucleic acids. In Peacocke’s ...
Gene replacement with the human BRCA1 locus
... otherwise lethal phenotype observed in the homozygous Brca17/7 mice. We isolated human BRCA1 protein from these lines and found a correlation between expression level and rescue. Furthermore, the protein was correctly translated and stabilized following exposure to DNA damage. From these results, it ...
... otherwise lethal phenotype observed in the homozygous Brca17/7 mice. We isolated human BRCA1 protein from these lines and found a correlation between expression level and rescue. Furthermore, the protein was correctly translated and stabilized following exposure to DNA damage. From these results, it ...
Could distal MSH2 upstream deletions cause HNPCC?
... The primer set for MSH2P4 is 10 kb upstream of MSH2 ...
... The primer set for MSH2P4 is 10 kb upstream of MSH2 ...
A Single Eubacterial Origin of Eukaryotic
... were used to amplify a fragment of 1,539 bp, which was cloned into pGEMT (Promega). Two clones were sequenced on both strands. To distinguish between this PCR product and the published G. lamblia PFO gene sequence, we refer to the published sequence as G. lamblia PFO1 and to the gene represented by ...
... were used to amplify a fragment of 1,539 bp, which was cloned into pGEMT (Promega). Two clones were sequenced on both strands. To distinguish between this PCR product and the published G. lamblia PFO gene sequence, we refer to the published sequence as G. lamblia PFO1 and to the gene represented by ...
Gene Expression Profiles of Cold
... broaden our current knowledge of transcripts present in the mature pollen grain. However, further gene expression and functional analyses of individual transcripts (da Costa-Nunes and Grossniklaus 2003), as well as the establishment and characterization of the gene expression profiles in germinating ...
... broaden our current knowledge of transcripts present in the mature pollen grain. However, further gene expression and functional analyses of individual transcripts (da Costa-Nunes and Grossniklaus 2003), as well as the establishment and characterization of the gene expression profiles in germinating ...
Provisional PDF
... losses (Shen et al., 2015). For example, hepatopancreatic necrosis disease (HPND, “shuibiezi” in Chinese), which affects E. sinensis, has been a major problem in the crab-cultivated area of Jiangsu Province since 2015. Crabs with HPND exhibit multiple clinical symptoms, including hepatopancreata tha ...
... losses (Shen et al., 2015). For example, hepatopancreatic necrosis disease (HPND, “shuibiezi” in Chinese), which affects E. sinensis, has been a major problem in the crab-cultivated area of Jiangsu Province since 2015. Crabs with HPND exhibit multiple clinical symptoms, including hepatopancreata tha ...
Partial report - GEP Community Server
... Complete report form for Models" each geneoninthe your project. Copy and paste this form tobe create as because of misannotations or because of updates to the D. melanogaster gene models from FlyBase. many copies as needed within this report. This could result in situations where you will need to co ...
... Complete report form for Models" each geneoninthe your project. Copy and paste this form tobe create as because of misannotations or because of updates to the D. melanogaster gene models from FlyBase. many copies as needed within this report. This could result in situations where you will need to co ...
DNA supercoiling factor contributes to dosage
... dramatic reduction in the viability of males carrying both GAL4 and UAS-IRscf (Fig. 1B). Most of these males died during the pupal stage, and the few individuals that did survive to adulthood died shortly after eclosion. The effects on male viability do not appear to be due to position effects, as t ...
... dramatic reduction in the viability of males carrying both GAL4 and UAS-IRscf (Fig. 1B). Most of these males died during the pupal stage, and the few individuals that did survive to adulthood died shortly after eclosion. The effects on male viability do not appear to be due to position effects, as t ...
Phytopathology
... hybridized spot and revealed a consistent pattern for multiple strains of each species or subspecies isolated from diverse geographical regions. In preliminary tests, bacteria could be correctly identified and detected by hybridizing to the array amplicons from mixed cultures and inoculated potato t ...
... hybridized spot and revealed a consistent pattern for multiple strains of each species or subspecies isolated from diverse geographical regions. In preliminary tests, bacteria could be correctly identified and detected by hybridizing to the array amplicons from mixed cultures and inoculated potato t ...
Loss of heterozygosity at D8S262: an early genetic event of
... of NAH without SCC and all NAH with SCC in liver cirrhosis tissue may represent monoclonal hyperplasia. The occurrence of SCC is a late event during NAH progression, and a premalignant morphologic phenotype [9]. These observations further support the contention that NAH is a precancerous lesion of H ...
... of NAH without SCC and all NAH with SCC in liver cirrhosis tissue may represent monoclonal hyperplasia. The occurrence of SCC is a late event during NAH progression, and a premalignant morphologic phenotype [9]. These observations further support the contention that NAH is a precancerous lesion of H ...
The EGF-CFC Protein One-Eyed Pinhead Is Essential for
... to double mutants for sqt and cyc, two zebrafish nodalrelated genes (Figure 1F). Mutant embryos display severe defects not seen in Zoep mutants, including lack of the notochord, pronephros, and blood. A variable number of somites that form in the tail region are the only nonectodermal structures det ...
... to double mutants for sqt and cyc, two zebrafish nodalrelated genes (Figure 1F). Mutant embryos display severe defects not seen in Zoep mutants, including lack of the notochord, pronephros, and blood. A variable number of somites that form in the tail region are the only nonectodermal structures det ...
A12_ScienceAndFiction - Collegiate Quiz Bowl Packet Archive
... effects of war on children. These charitable efforts were a natural outgrowth of her research into child psychology*. She is perhaps best known for “Food and Culture” in which she identified three categories of Infantile Feeding Disturbances: organic disturbances, instinctive processes, and neurotic ...
... effects of war on children. These charitable efforts were a natural outgrowth of her research into child psychology*. She is perhaps best known for “Food and Culture” in which she identified three categories of Infantile Feeding Disturbances: organic disturbances, instinctive processes, and neurotic ...
Horizontal gene transfer from flowering plants to Gnetum
... base pairs inserted before the GTGCG motif that is typical of the start of group II introns (Tables 3 and 4 and Figs. 1 and 6). Angiosperm-type nad1 exons found in Gnetum share five nucleotide substitutions with angiosperms that are not found in other seed plants. They are also characterized by a fo ...
... base pairs inserted before the GTGCG motif that is typical of the start of group II introns (Tables 3 and 4 and Figs. 1 and 6). Angiosperm-type nad1 exons found in Gnetum share five nucleotide substitutions with angiosperms that are not found in other seed plants. They are also characterized by a fo ...
Real-time PCR Handbook
... One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA, allowing the polymerase to synthesize nonspecific product. The problem of nonspecific products resulting from mis-priming can be ...
... One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA, allowing the polymerase to synthesize nonspecific product. The problem of nonspecific products resulting from mis-priming can be ...
The Nicotiana tabacum genome encodes two cytoplasmic
... (Marty and Meyer 1991). The hybridization and washing conditions were identical to those described in the protocols provided by Stratagene and Amersham. One positive lambda clone was purified. The Bluescript phagemid containing the cloned DNA insert was excised in vivo by coinfection with the helper ...
... (Marty and Meyer 1991). The hybridization and washing conditions were identical to those described in the protocols provided by Stratagene and Amersham. One positive lambda clone was purified. The Bluescript phagemid containing the cloned DNA insert was excised in vivo by coinfection with the helper ...
Gene Section ATF4 (activating transcription factor 4 (tax responsive enhancer element B67)) -
... hydroxylase) domain containing enzymes (PHD) [also called HIF Prolyl Hydroxylase, HPH], specifically PHD3. The betaTrCP recognition motif is another degradation motif, which when phosphorylated, is recognized by betaTrCP and targeted for proteasomal degradation. ...
... hydroxylase) domain containing enzymes (PHD) [also called HIF Prolyl Hydroxylase, HPH], specifically PHD3. The betaTrCP recognition motif is another degradation motif, which when phosphorylated, is recognized by betaTrCP and targeted for proteasomal degradation. ...
PCR Clean-up Kit / 96-well PCR Clean
... cosmid DNA (per well) from up to 1.3 ml of bacterial culture. This method is based on a modified alkaline lysis of the bacteria in combination with the selective binding of the plasmid DNA to a special silica membrane. The purified plasmid or cosmid DNA is free from protein, genomic DNA and RNA and ...
... cosmid DNA (per well) from up to 1.3 ml of bacterial culture. This method is based on a modified alkaline lysis of the bacteria in combination with the selective binding of the plasmid DNA to a special silica membrane. The purified plasmid or cosmid DNA is free from protein, genomic DNA and RNA and ...
Document
... Base your answers to questions 27 - 28 on the information and data table below and on your knowledge of biology. When a culture of cells is exposed to gamma rays, chromosome damage results. This damage is very evident when the cells are stained and observed with a compound light microscope. The chro ...
... Base your answers to questions 27 - 28 on the information and data table below and on your knowledge of biology. When a culture of cells is exposed to gamma rays, chromosome damage results. This damage is very evident when the cells are stained and observed with a compound light microscope. The chro ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.