Finding motifs in preomoters
... We used the Promoter Database of Saccharomyces cerevisiae. It contains genes and for every gene the TFs that are known to bind its promoter. We took 24 Transcription Factors whose PSWM is known, and 135 promoters of genes which are known to be bound by at least one of them. ...
... We used the Promoter Database of Saccharomyces cerevisiae. It contains genes and for every gene the TFs that are known to bind its promoter. We took 24 Transcription Factors whose PSWM is known, and 135 promoters of genes which are known to be bound by at least one of them. ...
Urine DNA Isolation Kit for Exfoliated Cells or Bacteria
... been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. The kit allows for the isolation of DNA from 1 to 50 mL of urine. The genomic DNA isolated from exfoliated cells can be used in a number of diagnostic and research applications including the diagnosis an ...
... been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. The kit allows for the isolation of DNA from 1 to 50 mL of urine. The genomic DNA isolated from exfoliated cells can be used in a number of diagnostic and research applications including the diagnosis an ...
Targeting Cell Division Cycle 7 Kinase
... such insults. Differently from other known DNA replication inhibitors, Cdc7 inhibition blocks DNA replication and induces apoptosis without eliciting the activation of the Chk1-dependent pathway. There is, in fact, growing evidence indicating that Cdc7 kinase activity is itself necessary for the act ...
... such insults. Differently from other known DNA replication inhibitors, Cdc7 inhibition blocks DNA replication and induces apoptosis without eliciting the activation of the Chk1-dependent pathway. There is, in fact, growing evidence indicating that Cdc7 kinase activity is itself necessary for the act ...
Protocol
... RNA interference (RNAi) refers to a mechanism by which double-strand RNAs (e.g. siRNA, shRNA, and miRNA) inhibit gene expression via nucleotide sequences complementary to the targeted messenger RNA, leading to mRNA degradation or translational repression (for reviews, see [1-7]). The discovery that ...
... RNA interference (RNAi) refers to a mechanism by which double-strand RNAs (e.g. siRNA, shRNA, and miRNA) inhibit gene expression via nucleotide sequences complementary to the targeted messenger RNA, leading to mRNA degradation or translational repression (for reviews, see [1-7]). The discovery that ...
Stimulation of nerve myelinating cell differentiation and potential for
... axonal integrity. In demyelinating diseases, such as Relapsing Remitting Multiple Sclerosis (MS), there is at first active remyelination (accounting for remissions) but when myelin loss exceeds repair there is progressive neuron death and brain ...
... axonal integrity. In demyelinating diseases, such as Relapsing Remitting Multiple Sclerosis (MS), there is at first active remyelination (accounting for remissions) but when myelin loss exceeds repair there is progressive neuron death and brain ...
Closely related proteins MBD2 and MBD3 play distinctive but
... To generate an Mbd2-mutant allele, exon 2 of the Mbd2 gene was replaced with the promoterless geo cassette (Fig. 2a). Transcription initiating at the promoter of the targeted Mbd2 locus will proceed as normal through exon 1 and intron 1 but then should terminate at the transcription stop site locat ...
... To generate an Mbd2-mutant allele, exon 2 of the Mbd2 gene was replaced with the promoterless geo cassette (Fig. 2a). Transcription initiating at the promoter of the targeted Mbd2 locus will proceed as normal through exon 1 and intron 1 but then should terminate at the transcription stop site locat ...
Key to RQ for Ex. 2
... therefore the regulatory action of rev protein is positive. (In other words, absence of rev protein blocks transport. If absence of rev protein caused constitutive transport, then regulation would be negative.) Explanation for 6B-3. The mRNA for gag protein can’t be spliced at the first pair of spli ...
... therefore the regulatory action of rev protein is positive. (In other words, absence of rev protein blocks transport. If absence of rev protein caused constitutive transport, then regulation would be negative.) Explanation for 6B-3. The mRNA for gag protein can’t be spliced at the first pair of spli ...
Construction and stable transformation of Tetrahymena
... of these genes in lifecycle by using functional complementation analysis based on phenotype with transfering genomic DNA library into mutant Tetrahymena cells using AC based vectors. There is no published literature describing an artificial chromosome for T.thermophila, Therefore, the aim of this st ...
... of these genes in lifecycle by using functional complementation analysis based on phenotype with transfering genomic DNA library into mutant Tetrahymena cells using AC based vectors. There is no published literature describing an artificial chromosome for T.thermophila, Therefore, the aim of this st ...
Breaking Down Cell-Cycle Barriers in the Adult Heart
... muscle was sufficient to promote DNA synthesis in adult ventricular myocytes in vitro and in vivo,10,16 supporting the role of Rb and related family members as key regulators of cell-cycle control in the heart. However, although these exciting early studies hinted toward the possibility of manipulat ...
... muscle was sufficient to promote DNA synthesis in adult ventricular myocytes in vitro and in vivo,10,16 supporting the role of Rb and related family members as key regulators of cell-cycle control in the heart. However, although these exciting early studies hinted toward the possibility of manipulat ...
Leukaemia Section t(6;12)(p21;p13) in lymphoid malignancies Atlas of Genetics and Cytogenetics
... Genes involved and Proteins ETV6 Location: 12p13 Note: The gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma. DNA / RNA 9 exons; alternate splicing. Protein The gene encodes an ETS family transcription factor; the produc ...
... Genes involved and Proteins ETV6 Location: 12p13 Note: The gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma. DNA / RNA 9 exons; alternate splicing. Protein The gene encodes an ETS family transcription factor; the produc ...
The Difference Makers
... researchers simulate, and what did they learn from their research? Possible student response: The researchers used cells from humans and chimpanzees and reprogrammed them to act like stem cells, and eventually, cranial neural crest cells, which help form the face. The process allowed the researchers ...
... researchers simulate, and what did they learn from their research? Possible student response: The researchers used cells from humans and chimpanzees and reprogrammed them to act like stem cells, and eventually, cranial neural crest cells, which help form the face. The process allowed the researchers ...
Hereditary Hyperferritinemia-Cataract Syndrome: Two Novel
... 1,389 µg/L but normal transferrin saturation. Similar abnormalities were noted in the proband’s sister, and liver biopsy specimens from both these individuals showed no iron overload. Sequencing of genomic DNA from the proband showed a heterozygous point mutation that corresponded to a 139 C = U sub ...
... 1,389 µg/L but normal transferrin saturation. Similar abnormalities were noted in the proband’s sister, and liver biopsy specimens from both these individuals showed no iron overload. Sequencing of genomic DNA from the proband showed a heterozygous point mutation that corresponded to a 139 C = U sub ...
Chapter 4: DNA and Chromosomes
... Lg number of transposable elements Lg average gene size 27,000 bp when only 1300 required to encode avg size protein Critical info seems to be in alarming state of disarray! ...
... Lg number of transposable elements Lg average gene size 27,000 bp when only 1300 required to encode avg size protein Critical info seems to be in alarming state of disarray! ...
Gene Section
... SMARCA4 somatic mutations have been identified in some cancer cell lines including those from the lung, prostate, breast, pancreas and colon. While somatic mutations have been detected in a small subset of lung primary tumors, about one third of the lung cancer cell lines of the non-small cell lung ...
... SMARCA4 somatic mutations have been identified in some cancer cell lines including those from the lung, prostate, breast, pancreas and colon. While somatic mutations have been detected in a small subset of lung primary tumors, about one third of the lung cancer cell lines of the non-small cell lung ...
1 BIOL 3200 Spring 2015 DNA Subway and RNA
... Part II: What is RNA-Seq and how does it work? As we have discussed in lecture, each cell in an organism contains the same DNA, i.e. the same genes, so how do organisms respond to developmental and environmental changes? They certainly cannot just make new genes on the fly. The best way is to regul ...
... Part II: What is RNA-Seq and how does it work? As we have discussed in lecture, each cell in an organism contains the same DNA, i.e. the same genes, so how do organisms respond to developmental and environmental changes? They certainly cannot just make new genes on the fly. The best way is to regul ...
Nucleic Acid Structures, Energetics, and Dynamics
... Analysis of DNA Sequence. The Human Genome Project is supported by NIH and DOE to identify all human genes and thus to revolutionize the diagnosis, prevention, and treatment of disease. Less than 10% of the human DNA codes for genes; most of the rest has no known function, although some is involved ...
... Analysis of DNA Sequence. The Human Genome Project is supported by NIH and DOE to identify all human genes and thus to revolutionize the diagnosis, prevention, and treatment of disease. Less than 10% of the human DNA codes for genes; most of the rest has no known function, although some is involved ...
The Arabinose Operon (http://faculty.clintoncc.suny.edu/faculty
... Ara C also binds to other sites within the operon, inhibiting transcription of the three structural genes. The genes therefore are normally not active. ...
... Ara C also binds to other sites within the operon, inhibiting transcription of the three structural genes. The genes therefore are normally not active. ...
S1 Table. Experimental evidence supporting interactions between
... cytokinins in the regulation of shoot and root meristem activity. The Plant cell. 15:2532-2550. Whitford, R., A. Fernandez, R. De Groodt, E. Ortega, and P. Hilson. 2008. Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells. Proceedings of the Nati ...
... cytokinins in the regulation of shoot and root meristem activity. The Plant cell. 15:2532-2550. Whitford, R., A. Fernandez, R. De Groodt, E. Ortega, and P. Hilson. 2008. Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells. Proceedings of the Nati ...
Nuclear Architecture, Chromosome Territories, Chromatin Dynamics
... combination with 3D-microscopy and image reconstruction is a key method to analyze the spatial arrangement of targeted DNA sequences in the nucleus. Using confocal microscopy that allows the distinct visualization of three or more different fluorochromes, differently labeled nuclear targets can be d ...
... combination with 3D-microscopy and image reconstruction is a key method to analyze the spatial arrangement of targeted DNA sequences in the nucleus. Using confocal microscopy that allows the distinct visualization of three or more different fluorochromes, differently labeled nuclear targets can be d ...
12) Inheritance, genes and chromosomes • 13) DNA
... • Genetic material is expressed as the phenotype— nucleotide sequence determines sequence of amino acids in proteins. ...
... • Genetic material is expressed as the phenotype— nucleotide sequence determines sequence of amino acids in proteins. ...
The DNA repair helicase UvrD is essential for replication
... • translocates 3' to 5' • can unwind from nicks or blunt ends if at high concentration • can unwind DNA/DNA and RNA/DNA duplexes • required for nucleotide excision repair • required for mismatch repair ...
... • translocates 3' to 5' • can unwind from nicks or blunt ends if at high concentration • can unwind DNA/DNA and RNA/DNA duplexes • required for nucleotide excision repair • required for mismatch repair ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.