Gene Expression-Based Assays for Cancers of Unknown Primary
... MiRview® mets (Rosetta Genomics, Philadelphia, PA) is another microarray technology which uses microRNAs (miRNA), small non-coding, single-stranded RNA molecules that regulate genes post-transcription, as a signature for tumor differentiation. The expression levels of these miRNAs have been shown to ...
... MiRview® mets (Rosetta Genomics, Philadelphia, PA) is another microarray technology which uses microRNAs (miRNA), small non-coding, single-stranded RNA molecules that regulate genes post-transcription, as a signature for tumor differentiation. The expression levels of these miRNAs have been shown to ...
Role of Tension and Twist in Single
... does not elastically deform the DNA, but transforms regions of the molecule from B-form DNA into an alternate structure [24]. Similarly, regime (iii) can be explained by the transition of part of the DNA to P-DNA with 2.6 bases per turn. This has been reported to occur at F 3 pN and a degree of su ...
... does not elastically deform the DNA, but transforms regions of the molecule from B-form DNA into an alternate structure [24]. Similarly, regime (iii) can be explained by the transition of part of the DNA to P-DNA with 2.6 bases per turn. This has been reported to occur at F 3 pN and a degree of su ...
Recurrent Tandem Gene Duplication Gave Rise
... Recurrent Tandem Gene Duplication Gave Rise to Functionally Divergent Genes in Drosophila Chuanzhu Fan,*1 Ying Chen, 2 and Manyuan Long* *Department of Ecology and Evolution, The University of Chicago; and The Committee on Genetics, The University of Chicago Tandem gene duplication is one of the m ...
... Recurrent Tandem Gene Duplication Gave Rise to Functionally Divergent Genes in Drosophila Chuanzhu Fan,*1 Ying Chen, 2 and Manyuan Long* *Department of Ecology and Evolution, The University of Chicago; and The Committee on Genetics, The University of Chicago Tandem gene duplication is one of the m ...
Solid Tumour Section Angiomatoid fibrous histiocytoma (AFH) Atlas of Genetics and Cytogenetics
... Angiomatoid fibrous histiocytoma (AFH)). Activation of the EWSR1-ATF1 oncogene is probably an early step in the transformation process, but the overall gene expression patterns are likely to vary considerably between AFH and CCS, in keeping with their clinopathologic differences. EWS/ATF1 functions ...
... Angiomatoid fibrous histiocytoma (AFH)). Activation of the EWSR1-ATF1 oncogene is probably an early step in the transformation process, but the overall gene expression patterns are likely to vary considerably between AFH and CCS, in keeping with their clinopathologic differences. EWS/ATF1 functions ...
Biology QUIZ: 13-2 and 13-3 Multiple Choice Identify the choice that
... ____ 19. In cystic fibrosis, a change in a single gene causes the protein called CFTR to a. become less soluble. b. fold improperly. c. destroy the cell membrane. d. transport sodium ions instead of chloride ions. ____ 20. Compared with normal hemoglobin, the hemoglobin of a person with sickle cell ...
... ____ 19. In cystic fibrosis, a change in a single gene causes the protein called CFTR to a. become less soluble. b. fold improperly. c. destroy the cell membrane. d. transport sodium ions instead of chloride ions. ____ 20. Compared with normal hemoglobin, the hemoglobin of a person with sickle cell ...
DNA Clean/Extraction Kit
... separate it into multiple tubes. 2) Cut large gel slices into several pieces to accelerate the gel dissolution. 3) Extend the incubation time to completely dissolve the gel slice. ...
... separate it into multiple tubes. 2) Cut large gel slices into several pieces to accelerate the gel dissolution. 3) Extend the incubation time to completely dissolve the gel slice. ...
Rhom-2 Expression Does Not Always Correlate With
... ern blot was prepared using BamHI-digested DNA. Hybridization of the Southern blot with a TCR-p probez4is shown in Fig 1A. As controls, DNAs from the KB and HPB cell lines were included. A germline band of 23 kb and two smaller rearranged bands for the TCR-p gene were observed for the Kl3 and HPB ce ...
... ern blot was prepared using BamHI-digested DNA. Hybridization of the Southern blot with a TCR-p probez4is shown in Fig 1A. As controls, DNAs from the KB and HPB cell lines were included. A germline band of 23 kb and two smaller rearranged bands for the TCR-p gene were observed for the Kl3 and HPB ce ...
FROM MOLECULAR PATTERNS TO MORPHOGENESIS THE LESSONS FROM DROSOPHILA
... In all species, many of the gene products present in the embryo are supplied by the mother during oogenesis. These include most RNAs and proteins needed for normal cell functions during early stages, as well as certain inhomogeneities that are used to define initial axes. In our experiments, homozyg ...
... In all species, many of the gene products present in the embryo are supplied by the mother during oogenesis. These include most RNAs and proteins needed for normal cell functions during early stages, as well as certain inhomogeneities that are used to define initial axes. In our experiments, homozyg ...
ANSWER - Issaquah Connect
... Reproduction : 10 - Answer False: All cells should have a controlled rate of cell division. Cancer cells have lost the ability to control division. Mutations have occurred within the genes that code for genes that regulate cell division. Can you remember which genes these are? ...
... Reproduction : 10 - Answer False: All cells should have a controlled rate of cell division. Cancer cells have lost the ability to control division. Mutations have occurred within the genes that code for genes that regulate cell division. Can you remember which genes these are? ...
trial by probability: bayes` theorem in court - UW
... cops, the evidence must have been planted. O.J. was found not guilty. ...
... cops, the evidence must have been planted. O.J. was found not guilty. ...
PTC_241Lecture005Cells
... used. Heterochromatin on the other hand is highly condensed. It’s all bound up very nicely with histones into those nucleosomes and it can’t be activated because of that and it’s very, very dark in color. So you might look at a nucleus during interphase and you might see some dark areas and then som ...
... used. Heterochromatin on the other hand is highly condensed. It’s all bound up very nicely with histones into those nucleosomes and it can’t be activated because of that and it’s very, very dark in color. So you might look at a nucleus during interphase and you might see some dark areas and then som ...
Identification of a novel streptococcal gene cassette mediating
... Cell viability was determined by counting the number of colonies. Using this protocol for the S. uberis ATCC BAA-854, the highest UV dose used (50 J/m2) was found to result in 20% (standard deviation, ⫾6.7%) cell survival, calculated from three independent experiments. To determine UV-induced antibi ...
... Cell viability was determined by counting the number of colonies. Using this protocol for the S. uberis ATCC BAA-854, the highest UV dose used (50 J/m2) was found to result in 20% (standard deviation, ⫾6.7%) cell survival, calculated from three independent experiments. To determine UV-induced antibi ...
Cell Division: Turning cell cycle entry on its head | eLife
... Figure 1. Revising the role of cyclin D/CDK in the regulation of Rb activity. (A) The cell cycle involves the following phases: the S-phase, during which DNA is duplicated, and the M-phase (or mitosis), during which chromosome segregation and cell division happen. A gap or G1-phase precedes the S-ph ...
... Figure 1. Revising the role of cyclin D/CDK in the regulation of Rb activity. (A) The cell cycle involves the following phases: the S-phase, during which DNA is duplicated, and the M-phase (or mitosis), during which chromosome segregation and cell division happen. A gap or G1-phase precedes the S-ph ...
A new ferrochelatase mutation combined with low
... fact, mutant cDNA containing the O1 mutation expressed in E. coli showed a significant amount of ferrochelatase protein with normal activity (Figure 6). In contrast, the O4 deletion, which is a 16 base pair deletion involving nucleotide base pairs 574–589, and results in translation into a truncated ...
... fact, mutant cDNA containing the O1 mutation expressed in E. coli showed a significant amount of ferrochelatase protein with normal activity (Figure 6). In contrast, the O4 deletion, which is a 16 base pair deletion involving nucleotide base pairs 574–589, and results in translation into a truncated ...
Ku Binds Telomeric DNA in Vitro - Titia de Lange Lab
... Loss of HDF1 or HDF2 has a dramatic effect on telomere position effect, equivalent to impairment of SIR2, SIR3, or SIR4 (34, 36). Second, although the nuclear localization of yeast Ku has not been determined directly, in HDF12 cells, the subnuclear localization of telomeres appears to be altered (37 ...
... Loss of HDF1 or HDF2 has a dramatic effect on telomere position effect, equivalent to impairment of SIR2, SIR3, or SIR4 (34, 36). Second, although the nuclear localization of yeast Ku has not been determined directly, in HDF12 cells, the subnuclear localization of telomeres appears to be altered (37 ...
The effect of DNA phase structure on DNA walks
... ratio than the whole genome (see Gardiner [3] for review). For the yeast genome the correlation between “coding density” and (G + C)/(A + T ) ratio has been shown by Sharp and Lloyd [11]). During transcription each strand of a DNA sequence can be read only in one direction in natural systems. The RN ...
... ratio than the whole genome (see Gardiner [3] for review). For the yeast genome the correlation between “coding density” and (G + C)/(A + T ) ratio has been shown by Sharp and Lloyd [11]). During transcription each strand of a DNA sequence can be read only in one direction in natural systems. The RN ...
Catalogue of Expressed Sequence Tags (ESTs) from
... allergens of other mite species. In additional, 7% ESTs show no significant similarity to any other DNA or protein sequences in the database. This lack of similarity to other sequences may indicate some role of these sequences, specific only to Acarus siro. This has opened up new possibilities in al ...
... allergens of other mite species. In additional, 7% ESTs show no significant similarity to any other DNA or protein sequences in the database. This lack of similarity to other sequences may indicate some role of these sequences, specific only to Acarus siro. This has opened up new possibilities in al ...
Tissue-Specific Expression and Promoter Analysis of the Tobacco
... LTPs so far cloned contain a leader sequence responsible for insertion into the ER and subsequent secretion of the protein (Bernhard et al., 1991; Madrid, 1991). In situ hybridizations have shown accumulation of Itp transcripts in epidermal layers of tobacco (Fleming et al., 1992), tomato (Fleming e ...
... LTPs so far cloned contain a leader sequence responsible for insertion into the ER and subsequent secretion of the protein (Bernhard et al., 1991; Madrid, 1991). In situ hybridizations have shown accumulation of Itp transcripts in epidermal layers of tobacco (Fleming et al., 1992), tomato (Fleming e ...
PDF
... set of three genes for a short biosynthetic chain that resulted in ‘yellow rice’. (They were fortunate in being able to introduce two of these genes at one time.) This chain of genes produced the enzymes required to enable rice grains to form b-carotene, which humans convert into vitamin A13. The co ...
... set of three genes for a short biosynthetic chain that resulted in ‘yellow rice’. (They were fortunate in being able to introduce two of these genes at one time.) This chain of genes produced the enzymes required to enable rice grains to form b-carotene, which humans convert into vitamin A13. The co ...
Word file (122 KB )
... All of the 24 pol30 mutants were in Plasmids pBL-230-x (ARS, CEN TRP1 pol30x). Sixteen mutants were kindly provided by Peter Burgers 1,2. We made six additional mutants based on information that the corresponding human PCNA mutants bound weakly to the human p150 (CAC1) in vitro compared to wild type ...
... All of the 24 pol30 mutants were in Plasmids pBL-230-x (ARS, CEN TRP1 pol30x). Sixteen mutants were kindly provided by Peter Burgers 1,2. We made six additional mutants based on information that the corresponding human PCNA mutants bound weakly to the human p150 (CAC1) in vitro compared to wild type ...
An overview of the structures of protein-DNA complexes
... only the Cro and Repressor and homeodomain families contain proteins with different amino-acid sequences. The pairwise sequence identities between the subunits in the Cro and Repressor family range from 68% (1lmbA and 1perA) to 100% for identical proteins (1lliA and 1lmbA). Pairwise SSAP scores are ...
... only the Cro and Repressor and homeodomain families contain proteins with different amino-acid sequences. The pairwise sequence identities between the subunits in the Cro and Repressor family range from 68% (1lmbA and 1perA) to 100% for identical proteins (1lliA and 1lmbA). Pairwise SSAP scores are ...
The Effects of Plasmids of Genotype and Phenotype
... independently of the bacterial chromosome, and many plasmids can also be transferred naturally among their bacterial hosts. Generally, plasmid genes code for proteins that are not essential for the survival of their host in its normal environment. However, some plasmid DNAs encode genes that confer ...
... independently of the bacterial chromosome, and many plasmids can also be transferred naturally among their bacterial hosts. Generally, plasmid genes code for proteins that are not essential for the survival of their host in its normal environment. However, some plasmid DNAs encode genes that confer ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.