Department of Drug Delivery Research
... chemotherapy to control the distribution of drugs and it is one of the most important fields and/or basic technologies supporting drug discovery and development in the pharmaceutical sciences associated with biomedicine and gene medicine. We have already developed a targeting system for macromolecul ...
... chemotherapy to control the distribution of drugs and it is one of the most important fields and/or basic technologies supporting drug discovery and development in the pharmaceutical sciences associated with biomedicine and gene medicine. We have already developed a targeting system for macromolecul ...
Genome_annotation
... •pilot phase focused on 30 Mb (~ 1%) of the genome •international consortium of computational and laboratory-based scientists working to develop and apply high-throughput approaches for detecting all sequence elements that confer biological function •now in its second phase, extending study to entir ...
... •pilot phase focused on 30 Mb (~ 1%) of the genome •international consortium of computational and laboratory-based scientists working to develop and apply high-throughput approaches for detecting all sequence elements that confer biological function •now in its second phase, extending study to entir ...
dilemmas regarding clinical obligation
... detection in heterozygous cases such as these, conveying of this information, and how it is done requires careful education and explanation. We believe these three examples are likely to be representative of multiple additional genes where clinical interpretation of aCGH results needs to be carefull ...
... detection in heterozygous cases such as these, conveying of this information, and how it is done requires careful education and explanation. We believe these three examples are likely to be representative of multiple additional genes where clinical interpretation of aCGH results needs to be carefull ...
Determining the Role of Wnt Signaling in Zebrafish
... followed by examining the changes in mRNA concentrations for β-catenin and Dishevelled over the course of oocyte maturation in order to determine the role and importance of the Wnt signaling pathway in this process. Changes in mRNA concentrations are determined through real-time RTPCR analysis. The ...
... followed by examining the changes in mRNA concentrations for β-catenin and Dishevelled over the course of oocyte maturation in order to determine the role and importance of the Wnt signaling pathway in this process. Changes in mRNA concentrations are determined through real-time RTPCR analysis. The ...
control of gene expression
... eukaryotes • This is much more complicated due to the numbers and arrangement of genes within the genome • Several genes may be responsible for one characteristic, and while they may be clustered together, the mechanisms that control them may be located on different chromosomes ...
... eukaryotes • This is much more complicated due to the numbers and arrangement of genes within the genome • Several genes may be responsible for one characteristic, and while they may be clustered together, the mechanisms that control them may be located on different chromosomes ...
Document
... DNA Polymerase – a protein complex that copies DNA to DNA RNA Polymerase – a protein complex that copies DNA to RNA Spliceosome – a protein/RNA complex that removes introns from pre-mRNA Ribosome – a protein/RNA complex that translates mRNA codons to amino acids, making proteins Intron – a non-codin ...
... DNA Polymerase – a protein complex that copies DNA to DNA RNA Polymerase – a protein complex that copies DNA to RNA Spliceosome – a protein/RNA complex that removes introns from pre-mRNA Ribosome – a protein/RNA complex that translates mRNA codons to amino acids, making proteins Intron – a non-codin ...
Name Ch 12 Study Guide
... 10) Assume that the two parent strands of DNA have been separated and that the base sequence on one parent strand is A-T-T-C-G-C; the base sequence that will complement that parent strand is __________________________________________ 11) Who was Rosalind Franklin? 12) What was her contribution to th ...
... 10) Assume that the two parent strands of DNA have been separated and that the base sequence on one parent strand is A-T-T-C-G-C; the base sequence that will complement that parent strand is __________________________________________ 11) Who was Rosalind Franklin? 12) What was her contribution to th ...
Gramene: A Resource for Comparative Grass Genomics
... Ontologies can be indexed “objectively” by a computer.\ Computers can infer new knowledge ...
... Ontologies can be indexed “objectively” by a computer.\ Computers can infer new knowledge ...
Document
... Junker and Schreiber, “Analysis of Biological Networks,” Wiley, 2008 West, “Introduction to graph theory,” 2nd edition, Prentice Hall, 2001 or T. Cormen et al., “Analysis of Algorithms”, 3rd edition, MIT press, 2009 A list of up-to-date research papers selected by the instructor: see ...
... Junker and Schreiber, “Analysis of Biological Networks,” Wiley, 2008 West, “Introduction to graph theory,” 2nd edition, Prentice Hall, 2001 or T. Cormen et al., “Analysis of Algorithms”, 3rd edition, MIT press, 2009 A list of up-to-date research papers selected by the instructor: see ...
Nature Biotechnology, 21(4) - Weizmann Institute of Science
... group regarding these parameters was not signifiaThe table divides the predicted 2,667 pairs according to the following two parameters: number of clusters in the pair (0, 1, or 2) that contain a known mRNA; and number of clusters in the pair (0, 1, or 2) that cantly different from that of the rest o ...
... group regarding these parameters was not signifiaThe table divides the predicted 2,667 pairs according to the following two parameters: number of clusters in the pair (0, 1, or 2) that contain a known mRNA; and number of clusters in the pair (0, 1, or 2) that cantly different from that of the rest o ...
DNA, RNA, and Central Dogma
... The amino acids sequence determines how the polypeptides will twist and fold into the protein. The shape of the protein is critical to its function. ...
... The amino acids sequence determines how the polypeptides will twist and fold into the protein. The shape of the protein is critical to its function. ...
High performance solution-based target selection using individually
... 5’-biotinylated 120nt oligonucleotide baits were also spiked into array-derived RNA baits to enhance coverage of high-GC regions. For these captures, either 1,000 oligo baits (~133kb target territory) or 3 oligo baits (1 exon) were mixed with the array/RNA baits; otherwise the conditions were equiva ...
... 5’-biotinylated 120nt oligonucleotide baits were also spiked into array-derived RNA baits to enhance coverage of high-GC regions. For these captures, either 1,000 oligo baits (~133kb target territory) or 3 oligo baits (1 exon) were mixed with the array/RNA baits; otherwise the conditions were equiva ...
When gene marriages don`t work out: divorce by subfunctionalization
... copies after gene duplication – provides an attractive explanation for why so many duplicated genes exist in eukaryotes, without requiring each duplication event to have conferred a selective advantage [1]. For many duplicated genes, however, it has been difficult to pinpoint different subfunctions ...
... copies after gene duplication – provides an attractive explanation for why so many duplicated genes exist in eukaryotes, without requiring each duplication event to have conferred a selective advantage [1]. For many duplicated genes, however, it has been difficult to pinpoint different subfunctions ...
BB30055: Genes and genomes
... Point mutations in single base pairs > 1.4million SNPs identified (~ 1 in every 1.9kb length on average) ~60,000 SNPs lie within exons and untranslated regions (85% of exons lie within 5kb of a SNP) May or may not affect the ORF (synonymous or non synonymous) Most SNPs may be regulatory ...
... Point mutations in single base pairs > 1.4million SNPs identified (~ 1 in every 1.9kb length on average) ~60,000 SNPs lie within exons and untranslated regions (85% of exons lie within 5kb of a SNP) May or may not affect the ORF (synonymous or non synonymous) Most SNPs may be regulatory ...
regulatory transcription factors
... There are two main types – General transcription factors • Required for the binding of the RNA pol to the core promoter and its progression to the elongation stage • Are necessary for basal transcription – Regulatory transcription factors • Serve to regulate the rate of transcription of nearby genes ...
... There are two main types – General transcription factors • Required for the binding of the RNA pol to the core promoter and its progression to the elongation stage • Are necessary for basal transcription – Regulatory transcription factors • Serve to regulate the rate of transcription of nearby genes ...
Genetic Variation
... • Cells have many smaller structures called organelles, that perform functions essential to life. • The nucleus of our cells contains our genetic information. ...
... • Cells have many smaller structures called organelles, that perform functions essential to life. • The nucleus of our cells contains our genetic information. ...
Supplementary Material and Methods
... the reaction was stopped by adding glycine to final concentration 0.2M for 10 minutes at room temperature. Fixed cells were rinsed twice with PBS and resuspended in lysis buffer (10 mM EDTA pH 8, 50 mM Tris-HCl pH 8, SDS 1 %). Lysate was sonicated 30 min (30 sec on / 30 sec off) in Diagenode water b ...
... the reaction was stopped by adding glycine to final concentration 0.2M for 10 minutes at room temperature. Fixed cells were rinsed twice with PBS and resuspended in lysis buffer (10 mM EDTA pH 8, 50 mM Tris-HCl pH 8, SDS 1 %). Lysate was sonicated 30 min (30 sec on / 30 sec off) in Diagenode water b ...
Microarray Protocol
... 6) Discard flow-through and add 2.5ml Buffer RPE, and centrifuge for 2 min. Add another 2.5 ml RPE, and repeat centrifuge for 5 min. 7) Place column in a new 15ml tube and add 250l DEPC water, let stand 1 min and centrifuge for 3 minutes to elute RNA (use only 150l water if sample is less than 150 ...
... 6) Discard flow-through and add 2.5ml Buffer RPE, and centrifuge for 2 min. Add another 2.5 ml RPE, and repeat centrifuge for 5 min. 7) Place column in a new 15ml tube and add 250l DEPC water, let stand 1 min and centrifuge for 3 minutes to elute RNA (use only 150l water if sample is less than 150 ...
WHY DO TUMOURS DEVELOP
... Causes a wide range of different types of damage to DNA Single and double stranded breaks Base damage Effects depend on quality of radiation and dose DNA repair mechanisms important Incorrect repair of DNA damage mutation ...
... Causes a wide range of different types of damage to DNA Single and double stranded breaks Base damage Effects depend on quality of radiation and dose DNA repair mechanisms important Incorrect repair of DNA damage mutation ...
Document
... proteins. • In human genome gene-dense regions contain about 1 gene per 10000 bases, with average estimated at 1 gene per 30000bases. • Therefore, corresponding error rate would be roughly one amino acid substitution in 100 proteins. • But large scale error in sequence assembly can also occur. Missi ...
... proteins. • In human genome gene-dense regions contain about 1 gene per 10000 bases, with average estimated at 1 gene per 30000bases. • Therefore, corresponding error rate would be roughly one amino acid substitution in 100 proteins. • But large scale error in sequence assembly can also occur. Missi ...
FocalScan: Scanning for altered genes in cancer based on
... by default scaled in relation to the median level of all nonzero expression values in the data set. A scale factor can be given to further adjust this value relative to the median. We found that a scale factor of 10 gave the best performance on average across different cancer data sets, which was th ...
... by default scaled in relation to the median level of all nonzero expression values in the data set. A scale factor can be given to further adjust this value relative to the median. We found that a scale factor of 10 gave the best performance on average across different cancer data sets, which was th ...
MicroRNAs as Oncogenes and Tumor Suppressors
... expression at the post-transcriptional level, the availability of a comprehensive microRNA expression profile could provide information that is complementary to that derived from mRNA transcriptional profiling. MicroRNAs are evolutionarily selected gene regulatory molecules. Therefore, microRNA expr ...
... expression at the post-transcriptional level, the availability of a comprehensive microRNA expression profile could provide information that is complementary to that derived from mRNA transcriptional profiling. MicroRNAs are evolutionarily selected gene regulatory molecules. Therefore, microRNA expr ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.