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rna polymerases
rna polymerases

... • RNA polymerase moves along the template strand in the 3' to 5' direction as it synthesizes the RNA product in the 5' to 3' direction using NTPs (ATP, GTP, CTP, UTP) as substrates. RNA polymerase does not proofread its work. The RNA product is complementary and antiparallel to the template strand. ...
Biosynthetic Pathway
Biosynthetic Pathway

... Northern blot analysis and quantitative RT-PCR. We have expressed all seven genes as GST fusion proteins in E. coli, and verified enzyme activity on the proposed substrates. We are conducting biochemical characterization to determine enzyme kinetics and substrate specificities. We are utilizing soyb ...
File - Perkins Science
File - Perkins Science

... unknown genotype with a homozygous recessive Protein: an organic compound composed of one or individual to determine the unknown genotype more chains of polypeptides, which in turn are Thymine: a nitrogen-containing base, one formed from amino acids component of a nucleotide, pairs with adenine Prot ...
Large-Scale High-Resolution Orthology Using Gene Trees
Large-Scale High-Resolution Orthology Using Gene Trees

... and Orthology Two genes in two species are orthologous if they derive from one gene in their last common ancestor • Orthologous genes are likely to have the same function • Much stronger than “tend to have similar function” ...
Matters of Sex - Old Saybrook Public Schools
Matters of Sex - Old Saybrook Public Schools

... No SRY gene = female Defective SRY gene = female 46, XY Rarely the SRY gene is translocated to an X chromosome 46, XX male ...
Chapter 17.
Chapter 17.

... From Gene to Protein ...
Chapter 17. - Cloudfront.net
Chapter 17. - Cloudfront.net

... From Gene to Protein ...
Proteins determine what?
Proteins determine what?

... vs ribose sugar 2. ACGT base pairs (DNA) vs. ACGU (RNA) 3. Double stranded (DNA) vs single stranded (RNA). 4. Helix(DNA) vs. Not usually helixed (RNA) ...
Gene Annotation
Gene Annotation

... •  Choose  a  gene  of  interest  from  Rice,  A.  thaliana  or  related  characterised  organism   •  Iden9fy  gene  homologs/orthologs  in  other  species  by  homology  based  BLAST  analysis   •  Obtain  protein  and  nucleo9de  sequences ...
Gene Disruption (cont) & Protein
Gene Disruption (cont) & Protein

... • Gene Disruption and Drug Discovery ...
as a PDF
as a PDF

... In practice, there was a catch. Although biologists had deciphered the code for translating DNA information into proteins, they could not yet read any natural DNA sequences--not even the sequence of a single gene out of the thousands present within a cell. They lacked the text on which to practice t ...
ppt - Computer Science & Engineering
ppt - Computer Science & Engineering

... • Dominant/Recessive gene: a dominant allele/ an allele that will be present only if it is present by itself • Genotype: genetic makeup of an individual cell • Phenotype: the overall effect of a gene • Homozygote: a diploid cell that has two copies of the same allele • Heterozygote : a diploid cell ...
TRPGR: Sequencing the barley gene-space
TRPGR: Sequencing the barley gene-space

... Chan et al., Barley sequencing discovery efforts in grass genomics. Thus, this barley GE sequencing initiative is the logical next step in the US commitment to the international effort to physically map and sequence the barley “gene space”. Another important preliminary step towards sequencing the ...
GENETICS I. Review of DNA/RNA – A. Basic Structure – DNA 3
GENETICS I. Review of DNA/RNA – A. Basic Structure – DNA 3

... 2. One cell that undergoes meiosis will yield how many independent cells? a) 1 b) 2 c) 3 d) 4 e) 5 3. In which generation of a monohybrid cross do all the individuals look the same? a) Parental (P) b) F1 c) F2 d) F3 4. In a monohybrid cross, the two parental genotypes are AA and aa. The genotype of ...
Protein Synthesis Powerpoint
Protein Synthesis Powerpoint

... 1. The mRNA molecule moves through a pore in the nuclear envelope and in to the cytoplasm. It joins with a ribosome and is translated one amino acid at a time. 2. ‘INITIATION’: the first codon on any mRNA molecule is called the ‘INITIATOR’. This codon is always AUG, which codes for the amino acid me ...
slides - ODU Computer Science
slides - ODU Computer Science

... • cDNA, vs. genomic DNA reverse transcriptase represent currently active mRNA population function, stage of the cell A cool animation http://www.maxanim.com/genetics/cDNA/cDNA.htm ...
A 3D pattern matching algorithm for DNA sequences
A 3D pattern matching algorithm for DNA sequences

... Biologists usually work with textual DNA sequences (A, C, G, T). Linear coding offers only a local and a onedimensional vision of the molecule. The 3D structure of DNA is known to be very important in many essential biological mechanisms. ...
STRUCTURAL CHROMOSOMAL ABERRATIONS Structural
STRUCTURAL CHROMOSOMAL ABERRATIONS Structural

... The next page continues looking at these chromosome mutations and mutations that happen within genes that can prove to be more harmful to the organism at hand. The following pages also investigates polyploidy in species. ...
Yeast Expression Vector (example) (baker’s yeast) LEU2 μ =  2 micron plasmid
Yeast Expression Vector (example) (baker’s yeast) LEU2 μ = 2 micron plasmid

... Protein DNA complexes migrate more slowly than naked DNA ...
Facts for Consumers - Physicians and Scientists for Global
Facts for Consumers - Physicians and Scientists for Global

... inserted into the genome of a new host. This is done by constructing a VECTOR that can invade the genome of the new host and insert the foreign DNA sequences into it. The inserted DNA sequence is usually made up of the TRANSGENE (the chosen genetic information), and a MARKER gene (usually conferring ...
Genetics IB Syllabus
Genetics IB Syllabus

...  Students should be able to recall one specific base substitution that causes glutamic acid to be substituted by valine as the sixth amino acid in the hemoglobin polypeptide.  The number of genes in a species should not be referred to as genome size as this term is used for the total amount of DNA ...
Slide 1
Slide 1

... given locus, neighboring genes are usually silent • CpG hypomethylation correlates with gene expression me ...
Discovering conserved DNA
Discovering conserved DNA

... How to Assign Targets for Enhancer Binding Transcription Factors? • Regulatory potential: sum of binding sites weighted by distance to TSS with exponential decay • Decay modeled from Hi-C experiments TSS ...
Biol 213 Genetics (13 September 2000) Relationship between
Biol 213 Genetics (13 September 2000) Relationship between

... You might view this transition warily, as an obstacle to overcome. After all, you’ve just gotten used to the way Brad does things, and here comes a new way. It’s true, Brad and I approach things somewhat differently. This not because one way is better than the other but rather because we each have d ...
Contemporary Biology Per
Contemporary Biology Per

... nucleotides that _____ the assembly of the new strand at certain places, allowing the pieces to be separated using gel electrophoresis. 26. The gel produces a _________ of bands that shows the sequence of the original DNA strand. This research is now completed and analyzed by computers. 27. This was ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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