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Problems X
Problems X

... and their relative abundances directly from short reads, allowing a profile of community metabolism and biomolecular functionality to be reconstructed in lieu of metagenome assembly. We validated this methodology using a collection of four synthetic metagenomes, determining the presence and abundanc ...
Human Genome Project - the Centre for Applied Genomics
Human Genome Project - the Centre for Applied Genomics

... “Sequencing” is the process of determining the specific order and identity of the three billion base pairs in the genome with the ultimate goal of identifying all of the genes. “Mapping” is the process of identifying discrete dna segments of known position on a chromosome which are then used for seq ...
Unit 5 Test Review 14-15
Unit 5 Test Review 14-15

... 10. During _____________________ the information carried by the mRNA is used to produce a protein. 11. The monomer of a protein is a(n): ___________________________________. 12. A polypeptide chain is a _______________________________. It is sometimes called a polypeptide chain because the nucleotid ...
Japanese Journal of Ophthalmology Vol.47 No.3
Japanese Journal of Ophthalmology Vol.47 No.3

... Background: Mutation of the human transforming growth factor beta-induced (TGFBI) gene causes granular corneal dystrophy (GCD) in various ethnic groups. In this report, we identify the genetic defect on the TGFBI gene in a Vietnamese family with atypical GCD. Cases: The patient and her relatives wer ...
The University of Oklahoma Health Sciences Center Laboratory for
The University of Oklahoma Health Sciences Center Laboratory for

... Polymorphism database (dbSNPs), which stores SNPs for many genes identified from the human genome project and other projects. Since Dr. Hallack’s course is focused on population genetics, the students learned how to obtain the genetic population data for the different alleles caused by SNPs. Lastly, ...
Genome & Protein “ Sequence Analysis Programs”
Genome & Protein “ Sequence Analysis Programs”

... a tool for correcting sequencing and basecaller errors using sequence alignment and chromatogram data. On average AutoEditor corrects 80% of erroneous base calls. It also greatly improves our ability to discover SNPs between closely related strains and isolates of the same species. ...
chapt16_lecture_edited [Compatibility Mode]
chapt16_lecture_edited [Compatibility Mode]

... At meiosis, one member of each chromosome pair segregates into one daughter nucleus and its homologue segregates into the other daughter nucleus. Each of the resulting haploid cells contains only one set of chromosomes. During the formation of haploid cells, the members of different chromosome pairs ...
Microarray data normalization and data transformation
Microarray data normalization and data transformation

... • Argument – subtracting background introduces significant variance into the estimation of hybridization intensity, decreasing the reliability • Although this may not produce any significant changes in relative expression levels between conditions for highly expressed genes, it can have profound eff ...
1.5 - Biology Junction
1.5 - Biology Junction

... Some genes use proteins that speed transcription. Sometimes regulation occurs at the level of protein synthesis. ...
Six3, a murine homologue of the sine oculis gene, demarcates the
Six3, a murine homologue of the sine oculis gene, demarcates the

... brain cDNA library was screened at low stringency using the previously isolated Six1 cDNA as probe. Approximately 106 plaques were screened and the positive clones were further purified. The largest cDNA insert isolated from these clones was of 1.8 kb (see Materials and Methods). Sequence analysis s ...
1 - BioMed Central
1 - BioMed Central

... Pairwise comparisons of chicken and zebra finch genes: A set of 3,653 chicken and zebra finch CDS pairwise alignments were examined to identify candidate genes potentially subject to directional selection. Ten candidate genes were observed with ω > 0.5 where the variable model was significantly favo ...
CS 2427 - Algorithms in Molecular Biology Lecture #2: 13 January
CS 2427 - Algorithms in Molecular Biology Lecture #2: 13 January

... to 3 direction, this tells us nothing about which way the fragment was originally oriented in the genome. In other words, the problem of putting all of the pieces together is complicated by the fact that we don’t know which strand each read came from; in fact, exactly half of the reads are from one ...
Evolution of prokaryotic genomes
Evolution of prokaryotic genomes

... dent of whether such change results in an alteration of involved in conjuration. These belong to the small autonphenotypic traits or not. omously replicating DNA molecules called plasmids. It It is a long experience of geneticists that mutations is interesting to note that these three basic processe ...
Gene Regulation - Lincoln Park High School
Gene Regulation - Lincoln Park High School

... in structure and function. Hox genes control the differentiation of cells and tissues in the embryo. ...
(lectures 5-7)  - Felsenstein/Kuhner lab
(lectures 5-7) - Felsenstein/Kuhner lab

... 4. Genetic drift leads ultimately to the population fixing for one allele or another. The A allele is either fixed or lost in each drifting population. 5. The analogy has been made to a “drunkard’s walk” in which the drunkard steps inaccurately and forgets where he has been. This will be demonstrate ...
Chapter12_Section05_edit-1
Chapter12_Section05_edit-1

... in structure and function. Hox genes control the differentiation of cells and tissues in the embryo. ...
DNA - Mrs. Barrett`s Biology Site
DNA - Mrs. Barrett`s Biology Site

... DNA profiling is a method of making a unique pattern of bands from the DNA of a person, which is used to distinguish that DNA from other DNA.  DNA is extracted from cells e.g. blood or semen by breaking up the cell membrane.  DNA amplification can be used if the quantity of DNA is low. Increasing ...
Presentation
Presentation

... caused by interactions of many genes and proteins and the environment. Alleles that cause genetic diseases may be inherited in a dominant or recessive pattern, and may be carried on autosomes or sex ...
Why Terminator technology won`t prevent GM
Why Terminator technology won`t prevent GM

... • There may be gene silencing or instability of one component leading to failure of the system. Depending on which gene was affected, there would be seed sterility at the wrong time (during seed production), or it would not occur when required (after sale to farmers). Gene silencing is one phenomeno ...
microRNA Targets in Stem Cell Differentiation
microRNA Targets in Stem Cell Differentiation

... MicroRNAs are non-coding RNAs that provide critical translational regulation within the cell1. This type of regulation occurs via base pairing of the microRNA (~ 22 nucleotides) to target sites in the 3' UTR of mammalian protein-coding genes, thus exerting control over a large proportion of the tran ...
2016 Midterm answer key
2016 Midterm answer key

... B) (EXTRA CREDIT - 2 pts) Assume that you screened the yeast library and found transformants that grow at 37C. Assume such temperature resistant colonies might arise due to two alternative possible mechanisms: 1) the presence of a complementing gene on the transformed plasmid or 2) from a chromosoma ...
Lecture 2
Lecture 2

... Definitions from the Language of Genetics Gene: The fundamental unit of heredity, which can be defined in three ways: i) A gene can be defined in molecular terms as a segment of DNA carrying the information necessary to express a complete protein or RNA molecule, including the promoter and coding s ...
Gene regulation in physiological stress
Gene regulation in physiological stress

... different environmental stresses, frequently highlighting previously unsuspected genes and proteins (and their cell functions) that participate in adaptive response. Easy entry into the study of virtually any gene/protein found in GenBank is available by using consensus sequences to design gene prim ...
Document
Document

... Next Step in Genomics • Transcriptomics involves large-scale analysis of messenger RNAs (molecules that are transcribed from active genes) to follow when, where, and under what conditions genes are expressed. • Proteomics—the study of protein expression and function—can bring researchers closer tha ...
Genetics 314 - Spring 2005
Genetics 314 - Spring 2005

... b) Starting to get frustrated, you decide to read the instructions. You read one warning saying that the kit will only work for genes with rho independent termination. What is meant by this and why would it possibly be a concern? The only potential problem here is if you were using a termination seq ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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