Non-coding RNAs - Biology Department | UNC Chapel Hill
... transcribed by RNA polymerase II and contain three important recognition elements (boxed): a 5′-trimethylguanosine (TMG) cap, an Sm-protein-binding site (Sm site) and a 3′ stem–loop structure. In metazoans, the Sm site and the 3′-stem elements are required for recognition by the survival motor neuro ...
... transcribed by RNA polymerase II and contain three important recognition elements (boxed): a 5′-trimethylguanosine (TMG) cap, an Sm-protein-binding site (Sm site) and a 3′ stem–loop structure. In metazoans, the Sm site and the 3′-stem elements are required for recognition by the survival motor neuro ...
Conservation ofParaHoxgenes` function in patterning of the
... Background: Presence of all three ParaHox genes has been described in deuterostomes and lophotrochozoans, but to date one of these three genes, Xlox has not been reported from any ecdysozoan taxa and both Xlox and Gsx are absent in nematodes. There is evidence that the ParaHox genes were ancestrally ...
... Background: Presence of all three ParaHox genes has been described in deuterostomes and lophotrochozoans, but to date one of these three genes, Xlox has not been reported from any ecdysozoan taxa and both Xlox and Gsx are absent in nematodes. There is evidence that the ParaHox genes were ancestrally ...
TriFecta Dicer-Substrate RNAi Manual
... In cells, small interfering RNAs (siRNAs) are produced by enzymatic cleavage of long dsRNAs by the RNase-III class endoribonuclease Dicer. The siRNAs associate with the RNA Induced Silencing Complex (RISC) in a process that is facilitated by Dicer. Dicer-Substrate RNAi methods take advantage of the ...
... In cells, small interfering RNAs (siRNAs) are produced by enzymatic cleavage of long dsRNAs by the RNase-III class endoribonuclease Dicer. The siRNAs associate with the RNA Induced Silencing Complex (RISC) in a process that is facilitated by Dicer. Dicer-Substrate RNAi methods take advantage of the ...
32 Fungal Genetics Newsletter Ursula Kües , Michaela J. Klaus
... monokaryons AT8 (A43, B43, trp-3, ade-8), FA2222 (A5 B6 acu-1 trp-1.1,1.6), LT2 (A6 B6 trp-1.1,1.6) and 218 (A3 B1 trp.1.1,1.6) (Kertesz-Chaloupková et al. 1998 Fungal Genet. Biol. 23 :95-109) as hosts and either plasmid pDB3 with the C. cinereus trp-3 + wild type gene (Burrows 1991 PhD Thesis, Lond ...
... monokaryons AT8 (A43, B43, trp-3, ade-8), FA2222 (A5 B6 acu-1 trp-1.1,1.6), LT2 (A6 B6 trp-1.1,1.6) and 218 (A3 B1 trp.1.1,1.6) (Kertesz-Chaloupková et al. 1998 Fungal Genet. Biol. 23 :95-109) as hosts and either plasmid pDB3 with the C. cinereus trp-3 + wild type gene (Burrows 1991 PhD Thesis, Lond ...
Molecular cloning and functional characterisation of a glucose
... We have cloned the first glucose transporter CaHGT1 (Candida albicans high-affinity glucose transporter) of a pathogenic yeast, Candida albicans. The DNA sequence (GenBank accession number Y16834) analysis revealed an ORF encoding a novel protein of 545 amino acids with a predicted molecular mass of ...
... We have cloned the first glucose transporter CaHGT1 (Candida albicans high-affinity glucose transporter) of a pathogenic yeast, Candida albicans. The DNA sequence (GenBank accession number Y16834) analysis revealed an ORF encoding a novel protein of 545 amino acids with a predicted molecular mass of ...
Transcription Networks
... regulate changes in expression profiles of specific genes. The transcription factors when bound change the probability per unit time of RNA polymerase binding to the promoter to produce the mRNA. Transcription factors can also function as activators which increase the transcription rate of a gene or ...
... regulate changes in expression profiles of specific genes. The transcription factors when bound change the probability per unit time of RNA polymerase binding to the promoter to produce the mRNA. Transcription factors can also function as activators which increase the transcription rate of a gene or ...
Relative Rates of Nucleotide Substitution in Frogs
... mtDNA substitution rates to a greater extent than scnDNA substitution rates (Rand 1994). Alternatively, the ratio of substitution rates may be more or less constant across divergent taxa, implying that evolution has arrived at some fundamental constant determined by the balance between error minimiz ...
... mtDNA substitution rates to a greater extent than scnDNA substitution rates (Rand 1994). Alternatively, the ratio of substitution rates may be more or less constant across divergent taxa, implying that evolution has arrived at some fundamental constant determined by the balance between error minimiz ...
Document
... What will the gene combinations be for these offspring? Copy this into your notebook and try to fill out the Punnett’s square. Continue when you are done. ...
... What will the gene combinations be for these offspring? Copy this into your notebook and try to fill out the Punnett’s square. Continue when you are done. ...
NCEA Level 2 Biology (91157) 2012 Assessment Schedule
... Gene pool is (all) the genes or alleles (held by the individuals) in a population. Mutation can be defined as a (permanent) change in the DNA. Somatic mutations occur in any cells of the body other than in the gametes Gametic mutations only occur in sex cells, eg, sperm /eggs (accept pollen). Explan ...
... Gene pool is (all) the genes or alleles (held by the individuals) in a population. Mutation can be defined as a (permanent) change in the DNA. Somatic mutations occur in any cells of the body other than in the gametes Gametic mutations only occur in sex cells, eg, sperm /eggs (accept pollen). Explan ...
This article appeared in a journal published by Elsevier. The
... N-terminal half of the 2b protein [9]. Based on RNA sequence data, CMV strains can be classified into three subgroups, IA, IB and II [20]. It has been found that subgroup I strains are, in general, more virulent than subgroup II strains and some studies have suggested that this differential virulence ...
... N-terminal half of the 2b protein [9]. Based on RNA sequence data, CMV strains can be classified into three subgroups, IA, IB and II [20]. It has been found that subgroup I strains are, in general, more virulent than subgroup II strains and some studies have suggested that this differential virulence ...
Robust Prediction of Expression Differences among Human
... computed and after correcting for multiple hypotheses testing, the set of SNPs that are significantly associated with the expression changes of every gene can be identified. Using this approach researchers were able to examine all of the SNPs measured in the human genome for significant associations ...
... computed and after correcting for multiple hypotheses testing, the set of SNPs that are significantly associated with the expression changes of every gene can be identified. Using this approach researchers were able to examine all of the SNPs measured in the human genome for significant associations ...
Genome browsers and other resources
... assembly and large-scale migration of genome annotations VectorBase: an updated bioinformatics resource for invertebrate vectors and other organisms related with human diseases SuperFlyL a comparative database for quantified spatio-temporal gene expression patterns in early dipteran embryos Do ...
... assembly and large-scale migration of genome annotations VectorBase: an updated bioinformatics resource for invertebrate vectors and other organisms related with human diseases SuperFlyL a comparative database for quantified spatio-temporal gene expression patterns in early dipteran embryos Do ...
Document
... from the ff container aside (petri dish) before beginning the next round. • 8. Count the F and f alleles (beans) that were placed in each of the "furred rabbit" dishes in the first round and record the number in the chart in the columns labeled "Number of F Alleles" and "Number of f Alleles." (This ...
... from the ff container aside (petri dish) before beginning the next round. • 8. Count the F and f alleles (beans) that were placed in each of the "furred rabbit" dishes in the first round and record the number in the chart in the columns labeled "Number of F Alleles" and "Number of f Alleles." (This ...
What happened to my genes? Insights on gene family dynamics
... number of genes separated by non-coding sequences (figure 1). Genes are delimited by predefined signaling sequences indicating transcription and translation start and stop. Transcription initiates at promoters, defined in the model as sequences that differ from an (arbitrarily chosen) 22-bp consensu ...
... number of genes separated by non-coding sequences (figure 1). Genes are delimited by predefined signaling sequences indicating transcription and translation start and stop. Transcription initiates at promoters, defined in the model as sequences that differ from an (arbitrarily chosen) 22-bp consensu ...
1. Interpreting rich epigenomic datasets
... Allele-specific chromatin marks: cis-vs-trans effects ...
... Allele-specific chromatin marks: cis-vs-trans effects ...
Document
... – Expression of sex-linked genes is similar to autosomal genes in females. – X chromosome inactivation randomly “turns off” one X chromosome – ensures that females, like males, have one functional copy of the X chromosome in each body cell ...
... – Expression of sex-linked genes is similar to autosomal genes in females. – X chromosome inactivation randomly “turns off” one X chromosome – ensures that females, like males, have one functional copy of the X chromosome in each body cell ...
Name: Chem 465 Biochemistry II - Test 3
... 12. In Chapter 24 you learned that much of the human genetic material consists of transposons. In Chapter 25 you learned that most transposons integrate using a recombination event. In Chapter 26 we learn that most eukariots transposons are retrotransposons. Put these three chapters together; what i ...
... 12. In Chapter 24 you learned that much of the human genetic material consists of transposons. In Chapter 25 you learned that most transposons integrate using a recombination event. In Chapter 26 we learn that most eukariots transposons are retrotransposons. Put these three chapters together; what i ...
Appendix: Fusion Gene Plasmid Construction
... was isolated from the pGEM-BAC 4.8 plasmid (3) as a BamH I - Bgl II fragment and subcloned into the BamH I - Bgl II digested -911 IGRP-CAT plasmid in the same orientation. The resulting plasmid contains IGRP promoter sequence from -1342 to +3, with a native IGRP Xba I restriction endonuclease site a ...
... was isolated from the pGEM-BAC 4.8 plasmid (3) as a BamH I - Bgl II fragment and subcloned into the BamH I - Bgl II digested -911 IGRP-CAT plasmid in the same orientation. The resulting plasmid contains IGRP promoter sequence from -1342 to +3, with a native IGRP Xba I restriction endonuclease site a ...
genetic mapping and dna sequencing of the loblolly pine genome
... markers had not yet been developed. RFLP probes derived from cDNA and genomic DNA were evaluated for use in loblolly pine. It was determined that as high, or higher, levels of polymorphism could be detected with cDNA probes than with genomic DNA probes (DEVEYet nl. 1991). Fur-thermore, cDNAs are the ...
... markers had not yet been developed. RFLP probes derived from cDNA and genomic DNA were evaluated for use in loblolly pine. It was determined that as high, or higher, levels of polymorphism could be detected with cDNA probes than with genomic DNA probes (DEVEYet nl. 1991). Fur-thermore, cDNAs are the ...
An Investigation of Codon Usage Bias Including
... small eukaryotic genomes. They can also be affected by such biases as those introduced by high or low GC-content [2]. In some cases these biases can coexist with translation bias [2, 8]. When this occurs translation bias can be obscured, making gene expression levels difficult to predict. Several ap ...
... small eukaryotic genomes. They can also be affected by such biases as those introduced by high or low GC-content [2]. In some cases these biases can coexist with translation bias [2, 8]. When this occurs translation bias can be obscured, making gene expression levels difficult to predict. Several ap ...
The DNA sequence of human chromosome 21
... matches arising from either cDNAs primed from intronic sites or repetitive elements frequently found in 59 or 39 untranslated regions. (3) Amino-acid similarities to known proteins or modular functional domains were considered to be signi®cant when an overall identity of greater than 25% over more t ...
... matches arising from either cDNAs primed from intronic sites or repetitive elements frequently found in 59 or 39 untranslated regions. (3) Amino-acid similarities to known proteins or modular functional domains were considered to be signi®cant when an overall identity of greater than 25% over more t ...
Jody Rosnik - ED591geneticslesson
... there is one dominant gene and one recessive gene in a gene pair, the color will be what the dominant gene says. This is the dominant/recessive relationship. Using the Punnett Square: To illustrate the combining of chromosomes scientists use a model called the Punnett Square. We can see through the ...
... there is one dominant gene and one recessive gene in a gene pair, the color will be what the dominant gene says. This is the dominant/recessive relationship. Using the Punnett Square: To illustrate the combining of chromosomes scientists use a model called the Punnett Square. We can see through the ...
7.2mb ppt - UCLA.edu
... deaminase (AID), which is expressed specifically in germinal-centre centroblasts1, is required for this process, but its exact role is unknown2. Here we show that AID is required for SHM in the centroblast-like Ramos cells, and that expression of AID is sufficient to induce SHM in hybridoma cells, w ...
... deaminase (AID), which is expressed specifically in germinal-centre centroblasts1, is required for this process, but its exact role is unknown2. Here we show that AID is required for SHM in the centroblast-like Ramos cells, and that expression of AID is sufficient to induce SHM in hybridoma cells, w ...
Translational Initiation in Eukaryotes
... codons in these mRNAs. So she engineered such a situation in the preproinsulin mRNA and tested its affect on translation. ...
... codons in these mRNAs. So she engineered such a situation in the preproinsulin mRNA and tested its affect on translation. ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.