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Over-expression of a putative poplar glycosyltransferase gene
Over-expression of a putative poplar glycosyltransferase gene

... several individual poplar glycosyltransferase genes have also been characterized. For example, Zhou et al. (2007) cloned two poplar glycosyltransferase genes, PoGT8D and PoGT43B (belonging to the GT8 and GT43 families, respectively) and found that they were associated with the secondary wall and inv ...
Ovule and embryo development, apomixis and fertilization Abdul M
Ovule and embryo development, apomixis and fertilization Abdul M

... the first cell division, in the outer integument cell enlargement occurs but cell divisions are not detected. Gametogenesis is arrested after the formation of tetrads. In the ino mutants the outer integuments are missing but the inner integuments develop normally. The development of the embryo sac i ...
The landscape of microbial phenotypic traits and associated genes
The landscape of microbial phenotypic traits and associated genes

... from the collection of differentially expressed genes during sporulation (52). For the analysis of conserved gene neighborhoods associated with sporulation, we considered only organisms belonging to the Firmicutes and Actinobacteria, as almost all known spore-forming bacteria are contained within. T ...
How Can Transposons Accelerate Your Genomics
How Can Transposons Accelerate Your Genomics

... • Express novel gene, or cloned library of genes, in E. coli background • Identify regulatory or functional regions, study protein-protein interactions Method ...
1st Lecture: Pro-‐ and an
1st Lecture: Pro-‐ and an

... studies  (short  life  cycle,  defined  gene;c  associated  phenotype,   conserved  pathways  and  responses  etc…)   5.  Many  of  the  C.  elegans  longevity/aging  pathways  are  conserved   with  higher  eukaryotes   6.  Aging  can  be  ar;fi ...
Example
Example

... Biologists have wasted a lot of time and effort in searching for all of the available information about each small area of research It is further hampered by the wide variations in terminology that may be common usage at any given time A simple example: if you were searching for new targets for anti ...
The percentage of bacterial genes on leading versus
The percentage of bacterial genes on leading versus

... levels versus genes on leading and lagging strands of E. coli, which has a substantial amount of microarray gene-expression data1. We found that the percentage of genes with similar expression levels on the leading strand increases as the expression level (averaged over all the available experimenta ...
GENETIC TRAITS
GENETIC TRAITS

... and Dad, but only certain genes get expressed and some traits require multiple genes. We call these sets of genes, alleles. • How do we know what trait we will have? We don’t, we only know what is definitely possible by looking at our parents; these are phenotypes. To know for sure the likelihood of ...
Coexpression of Linked Genes in Mammalian
Coexpression of Linked Genes in Mammalian

... probe set provides the best expression measure of a target gene (Liao and Zhang 2006a), we arbitrarily chose the probe set with highest expression level (Jordan et al. 2005), which was defined by the summation of S across all the examined tissues. As a result, 16,457 human and 16,134 mouse Ensembl g ...
Biochimica et Biophysica Acta
Biochimica et Biophysica Acta

... this system has been to identify protein variants that can bind to mutant RNA targets, hence providing detailed information about specific protein–RNA contacts [20,21]. This two-plasmid strategy was applied to the L30e protein and its target RNA. Although the initial two-plasmid constructs produced v ...
Effects of the Pattern of Energy Supply on the Efficiency of Nitrogen
Effects of the Pattern of Energy Supply on the Efficiency of Nitrogen

MUTATIONS
MUTATIONS

... oxidises a guanine base (see below). The oxidised guanine now binds with adenine instead of cytosine, and during subsequent interphase events, DNA polymerase will mistakenly create an A-T pairing in place of the original G-C pair at this point in the DNA molecule. ...
Genome engineering of mammalian haploid embryonic stem cells
Genome engineering of mammalian haploid embryonic stem cells

... cells contain only one copy of each chromosome and disruption of one allele can directly cause loss-of-function phenotypes. Recently, mouse haploid embryonic stem cells (ESCs) have been successfully generated, providing an ideal tool for genetic analyses (Elling et al., 2011; Leeb & Wutz, 2011). Ha ...
THE IDENTIFICATION AND CHARACTERISATION OF THE
THE IDENTIFICATION AND CHARACTERISATION OF THE

... The sequences of clones A-18 and A-66 were identical and determined by the NRPSpredictor 2 to be specific for phenylalanine. Protein BLAST analysis of the amino acid sequence of both clones showed a high similarity to an amino acid adenylation protein in Granuliella mallensis MP5ACIX8 (YP_00507340.1 ...
EPISTASIS
EPISTASIS

... maximum amount of melanin and very dark skin. Another way to think about it is to imagine that each capital letter allele makes one unit of melanin…by that logic, a skin cell with the genotype AABBCC would make 6 units of melanin and be dark. A genotype with all lower case allele (aabbcc) has no cap ...
Journal of Experimental Botany
Journal of Experimental Botany

... (rmc plants, kindly supplied by Drs Barker and Smith, Adelaide, Australia) was performed as described by Benabdellah et al. (1999) using a soil±sand-based inoculum of the AM fungus G. mosseae (Nicol. and Gerd.) Gerd. and Trappe (BEG119). Control plants received a ®ltrate (<20 mm) of the AM inoculum. ...
Splicing regulation: a structural biology perspective
Splicing regulation: a structural biology perspective

... Three chapters have been dedicated to this nuclear macromolecular machinery in human, yeasts and plants. Here, we focus on the large number of splicing factors involved in the regulation of splicing (also referred as alternative-splicing). Recent estimations indicate that nearly 80 to 95% of human m ...
Thrombocytopenia
Thrombocytopenia

... – ESPECIALLY MDS and AML • 40% of affected individual • Mean age of onset 33 years ...
Cancer Prone Disease Section Oculocutaneous Albinism Atlas of Genetics and Cytogenetics
Cancer Prone Disease Section Oculocutaneous Albinism Atlas of Genetics and Cytogenetics

... Oculocutaneous Albinism type 3 (OCA3) and Oculocutaneous Albinism type 4 (OCA4). OCA1 affects 1 per 40000 individuals in most populations (King et al., 2001) but is very uncommon among African-Americans. The overall prevalence of OCA2 is estimated to be 1:36000 in USA, but it is a lot more common am ...
Motif Finding
Motif Finding

... An Introduction to Bioinformatics Algorithms ...
Massachusetts Institute of Technology
Massachusetts Institute of Technology

... discrete as well as the continuous form. We will use two small java-applets that can “animate” the coalescent process, the Wright-Fisher animator (discrete generations) and the Hudson-animator (continuous time). These tools can both be accessed through http://www.coalescent.dk. It is important that ...
Analyzis of DNA Copy Number Aberrations with Chipster
Analyzis of DNA Copy Number Aberrations with Chipster

... and also reduces problems with multiple testing. In Chipster, this can be done with a tool called Identify common regions from called copy number data. These regions are what should be used for downstream analysis steps such as clustering, between-group comparisons, and survival analysis. The output ...
PPR2263, a DYW-Subgroup Pentatricopeptide
PPR2263, a DYW-Subgroup Pentatricopeptide

... the PPR proteins are predicted to be targeted to either mitochondria or chloroplasts, whereas no clear prediction is made for the rest. In addition to numerous experimental localizations in plastids and mitochondria, nuclear (Ding et al., 2006) or dual localization to mitochondria and nuclei (Hamman ...
2 Traits and Inheritance
2 Traits and Inheritance

... never exactly the same. The same gene might have more than one version. The different versions of a gene are called alleles. Alleles may be dominant or recessive. A trait for an organism is usually identified with two letters, one for each allele. Dominant alleles are given capital letters (A). Rece ...
COAT AND COLOUR GENES IN DACHSHUNDS
COAT AND COLOUR GENES IN DACHSHUNDS

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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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