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A Maize Glutaredoxin Gene, Abphyl2, Regulates
A Maize Glutaredoxin Gene, Abphyl2, Regulates

... had normal, alternate phyllotaxy (Figure 3A). We detected the YFPtagged ABPH2 protein in nuclei of young leaf primordia, but not in the central region of the meristem (Figure 3D). We did not observe YFP-ABPH2 in the meristem proper, including the P0, which may be due to the limited number of samples ...
unit3_lesson10_translation1_markscheme
unit3_lesson10_translation1_markscheme

... POD Mark Scheme Explain the translation of a protein from DNA [8]. ...
Natural selection and the function of genome imprinting:
Natural selection and the function of genome imprinting:

... homologues during DNA repair and recombination in both meiotic and mitotic cells. Maintenance of differences in chromatin structure in somatic cells can sometimes result in the transcription of only one allele at a locus. This pattern of transcription might be selected, in some instances, for reason ...
Agrobacterium-mediated transformation of banana Musa acuminata
Agrobacterium-mediated transformation of banana Musa acuminata

... transgenic plants at first generation (T0) of transformation. To prove the existence of hpt gene in the fruits of transgenic banana plants, PCR analysis were also carried out. The data showed that the hpt gene could be amplified from banana fruits of tested samples. These result demonstrates that th ...
Applicability of Yeast Genetics to Neurologic Disease
Applicability of Yeast Genetics to Neurologic Disease

... Mark William Walberg, MD, PhD ...
Solving Multiple Sequence Alignment Problems using Various E
Solving Multiple Sequence Alignment Problems using Various E

... Bioinformatics is hypothesizing biology in terms of molecules (in the sense of physical chemistry) and then applying informatics techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with these molecules, on a large-scale ...
MAGMA manual (version 1.06)
MAGMA manual (version 1.06)

... the p-value file that contains the sample size used per SNP. This option is recommended if such a column is present, especially when analysing SNP meta-analysis results where sample size per SNP can vary considerably. It should be noted that (at present) the specified sample size does not directly a ...
Human Germline Gene Therapy1
Human Germline Gene Therapy1

... with the exact, desired genetic change has finally been established. However, genetic changes can occur during long term culture, and there is evidence to suggest that subtle mutations are frequently induced in regions treated by targeted homologous recombination (6). Thus, it may be necessary to re ...
Risks from GMOs due to Horizontal Gene Transfer
Risks from GMOs due to Horizontal Gene Transfer

... et al., 2004), whereas the simplified conditions in laboratory studies probably lack many of the appropriate biotic and abiotic signals that facilitate HGT in nature (Mel and Mekalanos, 1996; Nielsen and van Elsas, 2001). For example, the presence of algae stimulates the release of bacterial plasmid ...
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR

... dishes were incubated at 30oC for one to three weeks. The separated colony was removed to PDA slant and incubated at 30oC until the rough and white light grey spores was appeared. The isolation process was repeated to obtain the pure isolate. Morphology, physiology and biochemical characteristics Af ...
The R2R3 MYB Transcription Factor GhMYB109 Is
The R2R3 MYB Transcription Factor GhMYB109 Is

... most important fiber crop for the textile industry. Current understanding of cotton fiber development is limited to computational and expression analyses of high-quality ESTs and the isolation and characterization of fiberrelated genes. Therefore, deciphering the molecular control of fiber developme ...
Huntington`s Disease Like-2: Review and Update
Huntington`s Disease Like-2: Review and Update

... protein level, long polyalanine repeats aggregate and are toxic in cell culture(42,43). HDL2 intranuclear inclusions might arise from expanded polyalanine or polyleucine repeats, which could adopt a conformation detectable by the 1C2 antibody. Another possibility is that the repeat expansion might a ...
A Primer on Gene Regulation
A Primer on Gene Regulation

... the electrophoretic mobility shift assay (Figure 8). A segment of DNA containing an operator site is subjected to electrophoresis through a gel in the presence of an electric field in which the negative pole is at one end (top end in the figure) of the gel and the positive pole at the other. The DNA ...
Clustering Genes using Heterogeneous Data Sources
Clustering Genes using Heterogeneous Data Sources

... Clustering of gene expression data is a standard exploratory technique used to identify closely related genes. Many other sources of data are also likely to be of great assistance in the analysis of gene expression data. Such sources include proteinprotein interaction data, transcription factor and ...
gene - Archbishop Ryan High School
gene - Archbishop Ryan High School

... One fourth (1/4) of the F2 plants have two alleles for tallness (TT); 2/4, or 1/2, of the F2 plants have one allele for tallness and one allele for shortness (Tt) Because the allele for tallness is dominant over the allele for shortness, 3/4 of the F2 plants should be tall Overall, there are 3 tall ...
Table S1.
Table S1.

... Confirmed segmental duplications, defined as having similarity to sequences in the Segmental Duplication Database (SDD) Duplications of at least 1 Kb of the total sequence (containing at least 500 bp of nonRepeatMasked sequence) with a sequence identity of at least ...
Comparison of two codon optimization strategies to enhance
Comparison of two codon optimization strategies to enhance

... Species-specific variations in codon usage are often cited as one of the major causes impacting protein expression levels [3, 4]. The presence of rare codons, which are correlated with low ...
Chapter 3
Chapter 3

... this common feature is the ancestral role of twist genes in triploblasts. In order to study whether twist is involved in mesoderm formation in lophotrochozoans, we set out to clone a putative twist ortholog of the gastropod mollusc Patella vulgata. We present here the cloning, characterization and t ...
Background and Overview of Comparative Genomics
Background and Overview of Comparative Genomics

... are derived from the majority noncoding and therefore more variable class of DNA (type I markers). However, these highly polymorphic markers are of very limited use for comparisons between genomes because their variability makes it impossible to detect homology across species. Although they may be e ...
Recombination and clonal groupings within Helicobacter pylori from
Recombination and clonal groupings within Helicobacter pylori from

... isolated from East Asians and Europeans (Atherton et al ., 1995; Ito et al ., 1998; Pan et al ., 1998; van Doorn et al ., 1998a), suggesting that recombination is rare between bacteria from different continents or that particular alleles are selected for in certain populations. However, a different ...
Near Neutrality, Rate Heterogeneity, and Linkage Govern
Near Neutrality, Rate Heterogeneity, and Linkage Govern

... 1999), bouts of adaptive evolution (McVean 2001), or mutational hot spots (Galtier et al. 2006). Nonetheless, the necessary enzymes for recombination are present in the mitochondria, and a few paternal mitochondria do penetrate the egg during fertilization (Thygarajan et al. 1996). Recombination is ...
video slide - Greenwood School District 50 / Overview
video slide - Greenwood School District 50 / Overview

... Colonies of cells containing the gene of interest have been identified by nucleic acid hybridization. Cells from colonies tagged with the probe can be grown in large tanks of liquid growth medium. Large amounts of the DNA containing the gene of interest can be isolated from these cultures. By using ...
Introduction to Genetics The Work of Gregor Mendel
Introduction to Genetics The Work of Gregor Mendel

... • Green seed color or yellow seed color • Tall plant or short plant ...
Isolation and Characterization of Rice MADS Box Gene
Isolation and Characterization of Rice MADS Box Gene

... C1032. Genomic DNA from 90 F 2 plants with dp-1 was prepared, and the polymorphism in the dp-1 phenotype was analyzed by using 3'-specific probes from C1032. However, dp-1 segregated from C1032 and thus does not correspond to C1032 for its phenotype (data not shown). E31864, which belongs to the API ...
Exon skipping and reading through stop codons
Exon skipping and reading through stop codons

... influence the progress of the disease with drug-like substances. But, with the exception of steroids, all this has not been done in living boys, but in their isolated muscle cells in the laboratory or in animals. However, clinical trials are being planned now for the near future, mainly trials with ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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