Package `Rsubread`
... (GT/AG), or cross different chromosomes or are located on different strands within the same chromosome, in addition to the canonical exon-exon junctions. Details The align function implements the Subread aligner (Liao et al., 2013) that uses a new mapping paradigm called “seed-and-vote". Subread is ...
... (GT/AG), or cross different chromosomes or are located on different strands within the same chromosome, in addition to the canonical exon-exon junctions. Details The align function implements the Subread aligner (Liao et al., 2013) that uses a new mapping paradigm called “seed-and-vote". Subread is ...
The use of the twin model to investigate the genetics and
... senescence in melanocytes have shown that patients with an excess of naevi have a delayed senescence in vitro.11 This finding suggests that different genes may have an effect on the appearance of naevi in early adulthood compared with the senescence process which occurs from the age of 40 years onwa ...
... senescence in melanocytes have shown that patients with an excess of naevi have a delayed senescence in vitro.11 This finding suggests that different genes may have an effect on the appearance of naevi in early adulthood compared with the senescence process which occurs from the age of 40 years onwa ...
Nutritional Genomics
... Maternal Met supplementation and embryo transcriptome 1. Lab work - total RNA extraction, amplification, library preparation, and sequencing (following Illumina mRNA-Seq protocol) 2. Map the reads to the reference genome 3. Reconstruct transcript models 4. Generate a final assembly merging together ...
... Maternal Met supplementation and embryo transcriptome 1. Lab work - total RNA extraction, amplification, library preparation, and sequencing (following Illumina mRNA-Seq protocol) 2. Map the reads to the reference genome 3. Reconstruct transcript models 4. Generate a final assembly merging together ...
Methylocapsa palsarum sp. nov., a Methanotrophic Bacterium from a
... cells of strain NE2T displayed well-developed intracellular membranes (ICM) packed in parallel ...
... cells of strain NE2T displayed well-developed intracellular membranes (ICM) packed in parallel ...
High-Throughput Neurotechnology
... 5. Steinmeyer, J., Gilleland, C., Pardo, C., Angel, M., and M. F. Yanik, “Construction of a femtosecond laser microsurgery system", Nature Protocols 5, 395 (2010). 6. Gilleland C., Rohde C., Zeng F., Yanik M. F., “Microfluidic immobilization of physiologically active Caenorhabditis elegans”, Nature ...
... 5. Steinmeyer, J., Gilleland, C., Pardo, C., Angel, M., and M. F. Yanik, “Construction of a femtosecond laser microsurgery system", Nature Protocols 5, 395 (2010). 6. Gilleland C., Rohde C., Zeng F., Yanik M. F., “Microfluidic immobilization of physiologically active Caenorhabditis elegans”, Nature ...
SFE1, a Constituent of the Fertilization Envelope
... tail. However, plaque hybridization experiments and 59 rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR) protocols to identify 59 coding regions were not successful in recovering any more than a few hundred bases 59 of the cysteine repeats, and the existing consensus sequence is ...
... tail. However, plaque hybridization experiments and 59 rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR) protocols to identify 59 coding regions were not successful in recovering any more than a few hundred bases 59 of the cysteine repeats, and the existing consensus sequence is ...
3. RESULTATS
... characterised CF alleles to 2.2%. Segregation studies of the mutations in the CF families allowed us to identify one case of a de novo mutation (L1065R) (Casals et al. 1997). The uniparental inheritance of CFTR microsatellite alleles allowed us to detect two large deletions (CFdel#1 and delE.3) (Mor ...
... characterised CF alleles to 2.2%. Segregation studies of the mutations in the CF families allowed us to identify one case of a de novo mutation (L1065R) (Casals et al. 1997). The uniparental inheritance of CFTR microsatellite alleles allowed us to detect two large deletions (CFdel#1 and delE.3) (Mor ...
Journal of Molecular Microbiology and Biotechnology
... mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Duitman et al., 1999], suggesting that additional NRPS operons could be integrated in different ways in this area either as an insertion or as a substitution of existing NRPS ope ...
... mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Duitman et al., 1999], suggesting that additional NRPS operons could be integrated in different ways in this area either as an insertion or as a substitution of existing NRPS ope ...
A wide-range phylogenetic analysis of Zic proteins: Implications for
... genes that appeared in ancestral Eumetazoa or metazoans, probably in the pre-Cambrian era, may have shared common structural features, namely the ZF and ZF-NC domains. Exon–intron boundaries are not conserved between Eumetazoa and cnidarians. Therefore, we postulate that the earliest Zic genes may n ...
... genes that appeared in ancestral Eumetazoa or metazoans, probably in the pre-Cambrian era, may have shared common structural features, namely the ZF and ZF-NC domains. Exon–intron boundaries are not conserved between Eumetazoa and cnidarians. Therefore, we postulate that the earliest Zic genes may n ...
Pelagia Research Library Exogenous gene transfer in Assam tea
... 0.65 % (w/v) agar (pH 5.6-5.8). After a month, somatic embryogenic calli derived from the cotyledons were transferred to SEM) containing half-strength basal MS salts, 20 g.L-1 sucrose, 0.65 % (w/v) agar, and 1 mg.L-1 BAP and 2 mg.L-1 NAA with pH 5.6-5.8. The secondary somatic embryos developed there ...
... 0.65 % (w/v) agar (pH 5.6-5.8). After a month, somatic embryogenic calli derived from the cotyledons were transferred to SEM) containing half-strength basal MS salts, 20 g.L-1 sucrose, 0.65 % (w/v) agar, and 1 mg.L-1 BAP and 2 mg.L-1 NAA with pH 5.6-5.8. The secondary somatic embryos developed there ...
Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics
... were compared with those of influenza B virus (15), approximately 60% homology was detected. The nucleotide sequences were analyzed by the maximum-parsimony method, and an evolutionary tree was constructed (Fig. 1). The diagram indicates four distinct lineages for the PB1 gene, the first comprising ...
... were compared with those of influenza B virus (15), approximately 60% homology was detected. The nucleotide sequences were analyzed by the maximum-parsimony method, and an evolutionary tree was constructed (Fig. 1). The diagram indicates four distinct lineages for the PB1 gene, the first comprising ...
Dragon Genetics 1 Teacher Prep
... that both sexes are equally likely to inherit an autosomal genetic condition such as sickle cell anemia. ...
... that both sexes are equally likely to inherit an autosomal genetic condition such as sickle cell anemia. ...
Advances in Environmental Biology (
... producers have easy access to other forms of auxiliary feeding [25]. These breeds are commonly found in a wide range of countries in Asia especially the Middle East and North Africa [3] .The study of genes underlying phenotypic variation can be performed in two different ways, first, from phenotype ...
... producers have easy access to other forms of auxiliary feeding [25]. These breeds are commonly found in a wide range of countries in Asia especially the Middle East and North Africa [3] .The study of genes underlying phenotypic variation can be performed in two different ways, first, from phenotype ...
multiplex rt-pcr reaction for simultaneous detection of tomato torrado
... labor-saving method. Diagnostics are then possible of more than one virus in one reaction tube, that can be used in a wider range of applications (Yin et al. 2012). As we have shown, three pairs of primers efficiently amplified targets: the partial sequence of Vp26, TGB, and GAPDH (Fig. 1) of ToTV, ...
... labor-saving method. Diagnostics are then possible of more than one virus in one reaction tube, that can be used in a wider range of applications (Yin et al. 2012). As we have shown, three pairs of primers efficiently amplified targets: the partial sequence of Vp26, TGB, and GAPDH (Fig. 1) of ToTV, ...
Myrick LK, Nakamoto-Kinoshita M, Lindor NM, Warren ST , et al. Fragile X syndrome due to a missense mutation. European Journal of Human Genetics (2014) 22, 1185-1189.
... established functions of FMRP in vitro. One of the penultimate consequences of FMRP loss is exaggerated AMPA receptor internalization.5 Cultured mouse hippocampal neurons derived either from wild-type (WT) or Fmr1 knockout (KO) mice have been shown to exhibit marked differences in AMPA receptor traf ...
... established functions of FMRP in vitro. One of the penultimate consequences of FMRP loss is exaggerated AMPA receptor internalization.5 Cultured mouse hippocampal neurons derived either from wild-type (WT) or Fmr1 knockout (KO) mice have been shown to exhibit marked differences in AMPA receptor traf ...
Flexibility in a Gene Network Affecting a Simple Behavior
... element is described by 60 average effects (at each of 60 time points), which are the deviations from the mean curve for the matrix. In order to generate a predicted curve for a transheterozygote based on additive effects, the average effects of each component are added to the grand mean data at eac ...
... element is described by 60 average effects (at each of 60 time points), which are the deviations from the mean curve for the matrix. In order to generate a predicted curve for a transheterozygote based on additive effects, the average effects of each component are added to the grand mean data at eac ...
Hierarchical Text Categorization and Its Evaluation
... • not a learning technique on its own, but a method in which a family of “weakly” learning agents (simple learners) is used for learning • based on the fact that multiple classifiers that disagree with one another can be together more accurate than its component classifiers • if there are L classifi ...
... • not a learning technique on its own, but a method in which a family of “weakly” learning agents (simple learners) is used for learning • based on the fact that multiple classifiers that disagree with one another can be together more accurate than its component classifiers • if there are L classifi ...
Origin and evolution of the slime molds (Mycetozoa)
... screening of whole colonies using M13 primers (19). For each amplification product, a minimum of five clones were further screened by partial sequencing (20). Final sequencing was done on an Applied Biosystems andyor Licor automatic sequencer. Both DNA strands were sequenced in their entirety, and a ...
... screening of whole colonies using M13 primers (19). For each amplification product, a minimum of five clones were further screened by partial sequencing (20). Final sequencing was done on an Applied Biosystems andyor Licor automatic sequencer. Both DNA strands were sequenced in their entirety, and a ...
proposal for complex variants
... According to the current HGVS recommendations, a single nucleotide substitution in the gene conversion and intronic insertion examples above would have to be described using the accession.version number of the sequence submitted to GenBank. Please note that the purpose of the extended description is ...
... According to the current HGVS recommendations, a single nucleotide substitution in the gene conversion and intronic insertion examples above would have to be described using the accession.version number of the sequence submitted to GenBank. Please note that the purpose of the extended description is ...
15_detaillectout
... homologous chromosomes at metaphase I of meiosis, which leads to the independent assortment of alleles. ...
... homologous chromosomes at metaphase I of meiosis, which leads to the independent assortment of alleles. ...
a heat-sensitive cellular function located in the nucleolus
... These lesions appear at 42°C and consist of a loss of the granular ribonucleoprotein (RNP) component and intranucleolar chromatin, and a disappearance of the nucleolar reticulum. The material remaining in the morphologically homogeneous nucleolus is a large amount of closely packed fibrillar RNP. Th ...
... These lesions appear at 42°C and consist of a loss of the granular ribonucleoprotein (RNP) component and intranucleolar chromatin, and a disappearance of the nucleolar reticulum. The material remaining in the morphologically homogeneous nucleolus is a large amount of closely packed fibrillar RNP. Th ...
Expression of an Entire Bacterial Operon in Plants
... Prokaryotic genes are usually clustered in operons under the control of a common promoter, and proteins are translated from a polycistronic mRNA (Jacob and Monod, 1961; Kozak, 1983; Salgado et al., 2000; Ermolaeva et al., 2001). Eukaryotic proteins are translated from their generally monocistronic ...
... Prokaryotic genes are usually clustered in operons under the control of a common promoter, and proteins are translated from a polycistronic mRNA (Jacob and Monod, 1961; Kozak, 1983; Salgado et al., 2000; Ermolaeva et al., 2001). Eukaryotic proteins are translated from their generally monocistronic ...
CHAPTER 17 FROM GENE TO PROTEIN Section C: The Synthesis
... transcription and translation in very similar ways, they do have differences in cellular machinery and in details of the processes. • Eukaryotic RNA polymerases differ from those of prokaryotes and require transcription factors. • They differ in how transcription is terminated. • Their ribosomes are ...
... transcription and translation in very similar ways, they do have differences in cellular machinery and in details of the processes. • Eukaryotic RNA polymerases differ from those of prokaryotes and require transcription factors. • They differ in how transcription is terminated. • Their ribosomes are ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.