Basics of Gene Expression Activity
... 13. Make an extension – using what you learned above, how could you dictate how much of a particular protein is made? Describe the “set-up” a cell might have in each case. a. To make a lot of a particular protein - _________________________________________________________ b. To make just a little - ...
... 13. Make an extension – using what you learned above, how could you dictate how much of a particular protein is made? Describe the “set-up” a cell might have in each case. a. To make a lot of a particular protein - _________________________________________________________ b. To make just a little - ...
Gene Expression
... Transcription is the process of creating RNA from DNA. Transcription occurs in the cell's nucleus. RNA polymerase is the protein molecule that reads the DNA and creates the RNA intermediary. Transcription requires: DNA, RNA polymerase, ribonucleotides, and some ATP for energy. Uracil (U) is substitu ...
... Transcription is the process of creating RNA from DNA. Transcription occurs in the cell's nucleus. RNA polymerase is the protein molecule that reads the DNA and creates the RNA intermediary. Transcription requires: DNA, RNA polymerase, ribonucleotides, and some ATP for energy. Uracil (U) is substitu ...
PROTIEN SYNTHESIS
... The form of RNA that mediates the transfer of genetic information from the cell nucleus to ribosomes in the cytoplasm, where it serves as a template for protein synthesis. It is synthesized from a DNA template during the process of transcription. transfer RNA One of a class of RNA molecules that tra ...
... The form of RNA that mediates the transfer of genetic information from the cell nucleus to ribosomes in the cytoplasm, where it serves as a template for protein synthesis. It is synthesized from a DNA template during the process of transcription. transfer RNA One of a class of RNA molecules that tra ...
Genetic Controls in Eukaryotes
... treated as exons and introns = different mRNA o Controlled by regulatory proteins specific to each cell type o Consequence = a single gene can code for more than one polypeptide = alternative RNA splicing o Humans can have fewer genes than proteins that are made. - mRNA degradation o How long mRNA s ...
... treated as exons and introns = different mRNA o Controlled by regulatory proteins specific to each cell type o Consequence = a single gene can code for more than one polypeptide = alternative RNA splicing o Humans can have fewer genes than proteins that are made. - mRNA degradation o How long mRNA s ...
PROTEIN SYNTHESIS QUESTIONS
... 2. The template strand of a gene contains the sequence 3’ TTCAGTCGT 5’. Draw the nontemplate sequence and the mRNA sequence, indicating 5’ and 3’ ends of each. Compare the two sequences. 3. Imagine that the nontemplate sequence in question 2 was traqnscribed instead of the template sequence. Draw th ...
... 2. The template strand of a gene contains the sequence 3’ TTCAGTCGT 5’. Draw the nontemplate sequence and the mRNA sequence, indicating 5’ and 3’ ends of each. Compare the two sequences. 3. Imagine that the nontemplate sequence in question 2 was traqnscribed instead of the template sequence. Draw th ...
Analysis of microarray data
... Analysis of microarray data • Normalization: Another source of noise is due to differences in labelling and detection efficiencies for the fluorescent labels and in the amount of RNA between the 2 samples (red/green). Normalization tries to get rid of this by dividing all the ratios by an appropria ...
... Analysis of microarray data • Normalization: Another source of noise is due to differences in labelling and detection efficiencies for the fluorescent labels and in the amount of RNA between the 2 samples (red/green). Normalization tries to get rid of this by dividing all the ratios by an appropria ...
Disease Identification
... 1.Mendelian/Monogenic Diseases : A mutation in just one of the genes ( 20,000-25,000) is responsible for disease i. Autosomal Recessive Single-Gene Diseases ii. Autosomal Dominant Single-Gene Diseases iii. X Chromosome–Linked Recessive Single-Gene Diseases iv. X Chromosome–Linked Dominant Single-Gen ...
... 1.Mendelian/Monogenic Diseases : A mutation in just one of the genes ( 20,000-25,000) is responsible for disease i. Autosomal Recessive Single-Gene Diseases ii. Autosomal Dominant Single-Gene Diseases iii. X Chromosome–Linked Recessive Single-Gene Diseases iv. X Chromosome–Linked Dominant Single-Gen ...
Lecture 15 Biol302 Spring 2011
... although possibly no more than accidental, that in all desoxypentose nucleic acids examined thus far the molar ratios of total purines to total pyrimidines were not far from 1. More should not be read into these figures.’’ Later in 1950, apparently as a last-minute insertion in the paper, Chargaff w ...
... although possibly no more than accidental, that in all desoxypentose nucleic acids examined thus far the molar ratios of total purines to total pyrimidines were not far from 1. More should not be read into these figures.’’ Later in 1950, apparently as a last-minute insertion in the paper, Chargaff w ...
protein synthesis notes
... tRNA w/ anticodon UAC and carrying a.a. methionine binds to start codon The next codon, in “A” site, binds w/ complimentary tRNA (carrying the corresponding a.a.) Enzyme forms a peptide bond between adjacent a.a. tRNA in “P” site now exits via “E” site and is ...
... tRNA w/ anticodon UAC and carrying a.a. methionine binds to start codon The next codon, in “A” site, binds w/ complimentary tRNA (carrying the corresponding a.a.) Enzyme forms a peptide bond between adjacent a.a. tRNA in “P” site now exits via “E” site and is ...
protein synthesis (simplified)
... It is the Sequence of bases that act like a code The sequence (order) of bases tells the cell what proteins to make. The sequence of bases dictates the sequence of amino acids, which determines the shape of a protein. ...
... It is the Sequence of bases that act like a code The sequence (order) of bases tells the cell what proteins to make. The sequence of bases dictates the sequence of amino acids, which determines the shape of a protein. ...
Introduction to Genetics and Genomics
... high-throughput techniques – development of bioinformatics -- (needs the use of computers to manage biological information) -direct consequence of more information available derived from genomics studies. ...
... high-throughput techniques – development of bioinformatics -- (needs the use of computers to manage biological information) -direct consequence of more information available derived from genomics studies. ...
Concept Check Questions with answers
... •Many nonidentical genes near each other •Different genes means different kinds of globin can be made at different stages of development ...
... •Many nonidentical genes near each other •Different genes means different kinds of globin can be made at different stages of development ...
replication (nucleus) transcription (nucleus) translation (cytoplasm
... A large transcription complex, including RNA polymerase and other proteins, assembles at the start of a gene and begins to unwind the DNA. Using one strand of the DNA as a template, RNA polymerase strings together a complementary strand of RNA. The RNA strand detaches from the DNA as it is transcri ...
... A large transcription complex, including RNA polymerase and other proteins, assembles at the start of a gene and begins to unwind the DNA. Using one strand of the DNA as a template, RNA polymerase strings together a complementary strand of RNA. The RNA strand detaches from the DNA as it is transcri ...
Supplementary Materials (doc 54K)
... For ALL cases #1-5, total RNA was isolated from primary ALL samples or splenocytes from transplanted mice (xenografts) using the RNeasy Mini Kit (Qiagen, Valencia, CA). Common leukemia translocations (i.e. TEL/AML1, BCR/ABL major and minor, E2A/PBX, MLL/AF4) were identified using the Signature® LTx ...
... For ALL cases #1-5, total RNA was isolated from primary ALL samples or splenocytes from transplanted mice (xenografts) using the RNeasy Mini Kit (Qiagen, Valencia, CA). Common leukemia translocations (i.e. TEL/AML1, BCR/ABL major and minor, E2A/PBX, MLL/AF4) were identified using the Signature® LTx ...
Chapter 13
... Process in which a ribosome reads the mRNA & makes a protein (polypeptide). Ribosome Structure: 1. Has two subunits: small & large 2. Large subunit has two sites: p site (polypeptide site) a site (amino acid site) ...
... Process in which a ribosome reads the mRNA & makes a protein (polypeptide). Ribosome Structure: 1. Has two subunits: small & large 2. Large subunit has two sites: p site (polypeptide site) a site (amino acid site) ...
Thomas Hunt Morgan`s Conclusions
... - modified Mendel’s work - used the fruit fly (Drosophila) to study inheritance Why use fruit flies? - Reproduce rapidly - Large number of offspring to study - Life cycle is only 10 – 15 days so it is possible to study many generations in a short period of time - Small size so many can fit into a sm ...
... - modified Mendel’s work - used the fruit fly (Drosophila) to study inheritance Why use fruit flies? - Reproduce rapidly - Large number of offspring to study - Life cycle is only 10 – 15 days so it is possible to study many generations in a short period of time - Small size so many can fit into a sm ...
5b Gene Expression
... - The Nature of Chromosomes - The Cell Cycle - Mitosis and Cytokinesis - Cancer Cells: Abnormal Cell Cycle • The Expression of Genes as Proteins: DNA gene --> RNA --> Protein - Transcription by RNA Polymerase (DNA gene --> mRNA) - The Three Types of RNA ...
... - The Nature of Chromosomes - The Cell Cycle - Mitosis and Cytokinesis - Cancer Cells: Abnormal Cell Cycle • The Expression of Genes as Proteins: DNA gene --> RNA --> Protein - Transcription by RNA Polymerase (DNA gene --> mRNA) - The Three Types of RNA ...
Gene Section FGA7 (Fused Gene 7 to AML1) in Oncology and Haematology
... transcripts. Top, diagram of AML1 shows the RUNT domain (RD) and the transactivation domain (TA) separated by exon 6. Center, the fusion of AML1 exon 6 to FGA7 results in the addition of 27 codons. The asterisks indicate the stop codon. Bottom, the alternative splice form that does not include exon ...
... transcripts. Top, diagram of AML1 shows the RUNT domain (RD) and the transactivation domain (TA) separated by exon 6. Center, the fusion of AML1 exon 6 to FGA7 results in the addition of 27 codons. The asterisks indicate the stop codon. Bottom, the alternative splice form that does not include exon ...
HOXA9 regulates expression of cancer stem cell markers and
... 服務單位:高雄醫學大學附設中和紀念醫院胃腸內科1 癌症中心2 高雄醫學大學藥學系3 高雄巿立小港醫院內科4 Background: Gastric cancer is one of the most common human cancers. According to Globocan 2000 estimates from World Health Organization (WHO), global gastric cancer incidence and mortality are 950,319 and 714,452 respectively. Nowadays, a growing ...
... 服務單位:高雄醫學大學附設中和紀念醫院胃腸內科1 癌症中心2 高雄醫學大學藥學系3 高雄巿立小港醫院內科4 Background: Gastric cancer is one of the most common human cancers. According to Globocan 2000 estimates from World Health Organization (WHO), global gastric cancer incidence and mortality are 950,319 and 714,452 respectively. Nowadays, a growing ...
Chorionic Gonadotropin (CG) 태반성 성선자극호르몬
... Primates and an Evolutionary History of Selection Glenn A. Maston & Maryellen Ruvolo Department of Anthropology, Harvard University, Cambridge, Massachusetts Abstract ...
... Primates and an Evolutionary History of Selection Glenn A. Maston & Maryellen Ruvolo Department of Anthropology, Harvard University, Cambridge, Massachusetts Abstract ...
Lecture 6
... • Gene finding is one of the first and most important steps in understanding the genome of a species once it has been sequenced. • In computational biology gene prediction or gene finding refers to the process of identifying the regions of genomic DNA that encode genes. – protein-coding genes – RNA ...
... • Gene finding is one of the first and most important steps in understanding the genome of a species once it has been sequenced. • In computational biology gene prediction or gene finding refers to the process of identifying the regions of genomic DNA that encode genes. – protein-coding genes – RNA ...
CH 13
... How does the mRNA tell us which amino acid comes next? •Each 3 bases in mRNA codes for one amino acid: ...
... How does the mRNA tell us which amino acid comes next? •Each 3 bases in mRNA codes for one amino acid: ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.