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No Slide Title
No Slide Title

... 2) An enzyme “slices” the DNA, making it single stranded. 3) Another enzyme (polymerase) synthesizes a strand of RNA using one of the DNA strands as a template. ...
Extracting Nucleic Acids from UK NEQAS LI Samples
Extracting Nucleic Acids from UK NEQAS LI Samples

... • It can be added directly to the lyophilised cells but will remain too viscous to do anything with unless they are left alone for at least five minutes ...
CHAPTER 36: RNA Synthesis and Regulation in Bacteria
CHAPTER 36: RNA Synthesis and Regulation in Bacteria

... • Encodes message from DNA to ribosomes • Rapidly degraded by nucleases ...
Ch. 17: From Gene to Protein
Ch. 17: From Gene to Protein

... recognition; transport 3) RNA splicing: exons (expressed sequences) kept introns (intervening sequences) are spliced out forming a spliceosome ...
1. A 6-frame translation map of a segment of DNA is shown, with
1. A 6-frame translation map of a segment of DNA is shown, with

... 2b. Complete your corrected diagram to show RNA transcripts of relatively correct length on the RNA polymerases. Transcription goes right-to-left through ORF B (because the Crick strand is the coding strand) so, the RNA transcripts will be smallest at the right (where transcription has just begun) a ...
Lecture slides
Lecture slides

... Gene regulation • Gene regulation is responsible for dynamic cell • Gene expression varies according to: ...
Mutation identification by whole genome sequencing
Mutation identification by whole genome sequencing

... and repeat multiple times 4) run in an analyzer to separate DNA products of different sizes and detect them by fluorescence 5) Obtain sequence 2. Next Generation Sequencing by the Illumina method a. Completed in a flow cell. 8 lanes on each cell can produce 12 billion bases of sequence information b ...
Chapter 5 part I
Chapter 5 part I

... • Large-scale, whole-genome experimentation, with the analysis of many samples at one time. • Proteomics – study of entire protein population of various cell types and tissues and numerous protein-protein interactions. • As new methods were implemented and research targets become more focused, other ...
Why teach a course in bioinformatics?
Why teach a course in bioinformatics?

... bothered to show up. Sounds like a pie-inthe-sky dream, doesn't it? But according to Victor Markovitz, vice president of bioinformatics systems at Gene Logic Inc., this actually happened at a recent biotech fair. And it is more or less typical of the prevailing global job market in bioinformatics an ...
DNA and RNA
DNA and RNA

... together by two types of bonds.  Phosphodiester bonds link the phosphate group of one nucleotide to the sugar of an adjacent nucleotide along the side of the double helix.  The nitrogenous bases are held together by hydrogen bonds across a rung. ...
Slide 1
Slide 1

... More than Error Correction?  Can correct biases from vastly different experiments  Aggregate data from multiple labs across multiple time points  Analyze and incorporate new data as it comes in  Metagenes discovered in vitro can be used as in vivo ...
Chapter 13: RNA and Protein Synthesis
Chapter 13: RNA and Protein Synthesis

... • Removal of entire chromosome ...
Introduction to Genomics - Department of Microbiology and Plant
Introduction to Genomics - Department of Microbiology and Plant

... Sequencing of genomes Computational and comparative genomics; Organization and evolution of genomes; SNPs Functional genomics: characterization of the function of the genes; molecular studies of gene expression Introduction to the concept of “epigenome” ...
Microarrays - Arizona State University
Microarrays - Arizona State University

1 BIOL 213 Fifth Exam All atoms, chemical bonding and structures
1 BIOL 213 Fifth Exam All atoms, chemical bonding and structures

... Text). "Whereas the general transcription factors that assemble at the promoter are the same for all genes transcribed by RNA polymerase II, the gene regulatory proteins and the locations of their binding sites are different for different genes." ...
Workflow for processing high throughput Single Molecule Real
Workflow for processing high throughput Single Molecule Real

... There are many sequencing-based approaches to understanding complex metagenomic communities spanning targeted amplification to whole-sample shotgun sequencing. While targeted approaches provide valuable data at low sequencing depth, they are limited by primer design and PCR. Whole-sample shotgun exp ...
Solid Tumor
Solid Tumor

... NUT TFE3 c-MYC ...
Complementary DNA Sequencing: Expressed Sequence Tags and
Complementary DNA Sequencing: Expressed Sequence Tags and

... • Found 230 ESTs, representing new genes • Random selection approach yields a high amount of highly represented clones in the cDNA libraries used ~ NOT GOOD!! • EST and physical mapping → high resolution map of the location of genes on chromosomes ~ more efficient and cheaper than genomic sequencing ...
Exam II Study Guide  Chapter 8:  Cellular Reproduction cell cycle
Exam II Study Guide Chapter 8: Cellular Reproduction cell cycle

... either increase or decrease the expression of that gene, including transcription, RNA processing, and translation. Regulating transcription: regulatory proteins called transcription factors (which can be activators or repressors) bind to control sequences around eukaryotic genes (such as the promote ...
Notes Unit 4 Part 7
Notes Unit 4 Part 7

... 5. The remaining pieces of RNA, known as exons, are _______________ together to form the completely complementary strand of RNA known as mRNA  exons = DNA sequences that ________ for proteins *** What would be the base sequence of mRNA if DNA is CGTAATTGCGCA?*** D. Translation = the decoding of the ...
Document
Document

... The order of the nitrogen bases along a gene forms a genetic code that specifies what type of protein will be produced.  DNA—double helix o sugar, phosphates, nitrogen bases  chromosomesDNAorder of bases amino acids proteins EQ 10 How does a cell produce proteins? During protein synthesis, the ...
The evolution of life science methodologies: From single gene
The evolution of life science methodologies: From single gene

... (b) In shotgun sequencing with cyclic‐array methods, common  adaptors are ligated to fragmented genomic DNA, which is then  subjected to one of several protocols that results in an array of  millions of spatially immobilized PCR colonies or 'polonies'15.  Each polony consists of many copies of a sin ...
RNA STRUCTURE - mbbsclub.com
RNA STRUCTURE - mbbsclub.com

... attached to the 3′-end .This poly-A tail is not transcribed from the DNA, but is added after transcription.  These tails help stabilize the mRNA and facilitate their exit from the nucleus. After the mRNA enters the cytosol, the poly-A tail is gradually shortened ...
Transcription_12_Teacher
Transcription_12_Teacher

... Transcribed mRNA (pre-mRNA) must be modified before leaving the nucleus There are special “dividers” in the mRNA that need to be cut out. They are not part of the genetic information, just organizers. ...
The noncoding explosion
The noncoding explosion

... the genome has ­revolutionized the ability to probe biological systems. ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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