
Variations on a theme: Genomics of sex
... same coverage in males and females like autosomal region. This copy number variation strategy has previously been used to identify non-recombining regions on fish sex chromosomes [39, 40]. To identify male-limited loci, we built a RAD locus catalog from the father as a reference (instead of using th ...
... same coverage in males and females like autosomal region. This copy number variation strategy has previously been used to identify non-recombining regions on fish sex chromosomes [39, 40]. To identify male-limited loci, we built a RAD locus catalog from the father as a reference (instead of using th ...
Transition Bias
... numbers, your knowledge is of a meagre and unsatisfactory kind; it may be the beginning of knowledge, but you have scarcely in your thoughts advanced to the state of Science, whatever the matter may ...
... numbers, your knowledge is of a meagre and unsatisfactory kind; it may be the beginning of knowledge, but you have scarcely in your thoughts advanced to the state of Science, whatever the matter may ...
Exogenous glutamine increases lipid accumulation in
... (Qiagen, Valencia, CA) following the manufacturer’s protocol. cDNA libraries were constructed and then sequenced by Novogene (Beijing, China) using an Illumina HiSeq 2000 platform to generate single-end 50 bp reads. Sequencing raw data from each library was preprocessed using TopHat, allowing up to ...
... (Qiagen, Valencia, CA) following the manufacturer’s protocol. cDNA libraries were constructed and then sequenced by Novogene (Beijing, China) using an Illumina HiSeq 2000 platform to generate single-end 50 bp reads. Sequencing raw data from each library was preprocessed using TopHat, allowing up to ...
GENOTYPE-PHENOTYPE CORRELATION USING
... cells, screening mutant cells for a phenotype of interest and identifying mutated genes in affected cells. In the above example, Escherichia coli cells are randomly mutated, cells that show antibiotic resistance phenotype are selected, and mutated genes are identified. Reverse genetics entails intro ...
... cells, screening mutant cells for a phenotype of interest and identifying mutated genes in affected cells. In the above example, Escherichia coli cells are randomly mutated, cells that show antibiotic resistance phenotype are selected, and mutated genes are identified. Reverse genetics entails intro ...
Testing the ABC floral-organ identity model: expression of
... Only those cells in which both AP3 and PI are expressed in stage 3 will continue to express the genes in later stages. (AP3 and PI polypeptides have been shown to interact and bind the MADS-box DNA target sequence CC(A/T)6GG.) ...
... Only those cells in which both AP3 and PI are expressed in stage 3 will continue to express the genes in later stages. (AP3 and PI polypeptides have been shown to interact and bind the MADS-box DNA target sequence CC(A/T)6GG.) ...
14lctout - Evergreen Archives
... (1) Cells are exposed to mutagens and grown on glucose medium. (2) Each cell forms a colony. This is the "master plate." (3) A block covered with sterile velvet is pressed onto the master plate. (4) Some cells from each colony are transferred to the velvet. (5) The velvet is pressed onto a plate of ...
... (1) Cells are exposed to mutagens and grown on glucose medium. (2) Each cell forms a colony. This is the "master plate." (3) A block covered with sterile velvet is pressed onto the master plate. (4) Some cells from each colony are transferred to the velvet. (5) The velvet is pressed onto a plate of ...
Evolution of the Actin Gene Family in Testate Lobose Amoebae
... PCR amplification yields artifactual recombinants (chimeras) when multiple closely related target sequences are present in the mixture (Judo et al. 1998). We chose to rely on an empirical strategy, performing extensive PCR-artifact formation experiments in our study system to determine chimera-reduc ...
... PCR amplification yields artifactual recombinants (chimeras) when multiple closely related target sequences are present in the mixture (Judo et al. 1998). We chose to rely on an empirical strategy, performing extensive PCR-artifact formation experiments in our study system to determine chimera-reduc ...
The nucleotide sequence of Saccharomyces cerevisiae
... PMA1 and ATE1 loci. Analysis of these data led to the estimation of a minimum number of 5,300 expressed genes in yeast. In this centromeric region a recombination frequency of 1 cM corresponded to an average distance of 3.3 kb, compared with 2.9 kb for the complete chromosome. These extrapolations h ...
... PMA1 and ATE1 loci. Analysis of these data led to the estimation of a minimum number of 5,300 expressed genes in yeast. In this centromeric region a recombination frequency of 1 cM corresponded to an average distance of 3.3 kb, compared with 2.9 kb for the complete chromosome. These extrapolations h ...
Synonymous Codon Usage, Accuracy of Translation, and Gene
... hypotheses could be proposed to explain this observation. Akashi (1994) tested mutational alternatives that could potentially explain the higher frequency of optimal codons at constrained codons than at nonconstrained ones, and all of them were clearly rejected. We could not test these mutational hy ...
... hypotheses could be proposed to explain this observation. Akashi (1994) tested mutational alternatives that could potentially explain the higher frequency of optimal codons at constrained codons than at nonconstrained ones, and all of them were clearly rejected. We could not test these mutational hy ...
A dominant mutation in the gene for the Nag
... Methods Bacteriologicalmethods. The E. coli strains used in this work are listed in Table 1. The nags-'lac2 fusion carried by a Ad857 transducing phage, 1NagB-lacZ, and its use in measuring expression of the nag regulon have been described previously (Plumbridge, 1990). The same fusion transferred t ...
... Methods Bacteriologicalmethods. The E. coli strains used in this work are listed in Table 1. The nags-'lac2 fusion carried by a Ad857 transducing phage, 1NagB-lacZ, and its use in measuring expression of the nag regulon have been described previously (Plumbridge, 1990). The same fusion transferred t ...
Wolstencroft
... involved in the ability of mice to expel the parasite. Manual experimentation: Two year study of candidate genes, processes unidentified ...
... involved in the ability of mice to expel the parasite. Manual experimentation: Two year study of candidate genes, processes unidentified ...
16S rRNA characterization of Bacillus strain and its
... The isolation of microorganisms from different highly contaminated environments offers novel bacteria of unique functionality and potential applications in different biotechnological processes. It also entails a variety of genes responsible for microbial tolerance or defense against extreme conditio ...
... The isolation of microorganisms from different highly contaminated environments offers novel bacteria of unique functionality and potential applications in different biotechnological processes. It also entails a variety of genes responsible for microbial tolerance or defense against extreme conditio ...
ACEMBL System:
... Acceptor vectors, and in fact the entire multiple integration element (MIE) can be exchanged with a favored expression cassette by using restriction/ligation cloning with appropriate enzymes (for example ClaI/PmeI, Illustration 2) or insertion into linearized ACEMBL vectors where the MIE was removed ...
... Acceptor vectors, and in fact the entire multiple integration element (MIE) can be exchanged with a favored expression cassette by using restriction/ligation cloning with appropriate enzymes (for example ClaI/PmeI, Illustration 2) or insertion into linearized ACEMBL vectors where the MIE was removed ...
YEAST GENETICS AND MOLECULAR BIOLOGY
... One such haploid-specific gene is RME, encoding the repressor of meiosis. Although it is not expressed in diploids the meiosis and sporulation programme will only start once nutrients become limiting Taken together, cell type is determined with very few primary transcription factors that act individ ...
... One such haploid-specific gene is RME, encoding the repressor of meiosis. Although it is not expressed in diploids the meiosis and sporulation programme will only start once nutrients become limiting Taken together, cell type is determined with very few primary transcription factors that act individ ...
Resistance to cephalosporins and carbapenems in Gram
... the acyl–enzyme complex. There is sufficient evidence that class A -lactamases perform several conformational changes induced by substrate binding and substrate reaction. In parallel, most substrates undergo chemical rearrangements triggered by the enzyme attack which can lead to more complex kineti ...
... the acyl–enzyme complex. There is sufficient evidence that class A -lactamases perform several conformational changes induced by substrate binding and substrate reaction. In parallel, most substrates undergo chemical rearrangements triggered by the enzyme attack which can lead to more complex kineti ...
Splicing together sister chromatids
... Figure 1. The control of sororin levels by the spliceosome determines proper sister chromatid cohesion during mitosis. The protein levels of sororin, encoded by the CDCA5 gene in humans, depend on the proper splicing of CDCA5 pre-mRNAs in interphase. After replication (S-phase), the cohesin ring mai ...
... Figure 1. The control of sororin levels by the spliceosome determines proper sister chromatid cohesion during mitosis. The protein levels of sororin, encoded by the CDCA5 gene in humans, depend on the proper splicing of CDCA5 pre-mRNAs in interphase. After replication (S-phase), the cohesin ring mai ...
Problems in Protein Biosynthesis - The Journal of General Physiology
... aminoacyl-tRNA (25, 26). For example, a poly U-30S ribosome subunit complex binds phenylalanyl-tRNA. A single codon, a triplet, is sufficient to direct specific binding of aminoacyl-tRNA to the ribosome (27). The growing polypeptide chain, which is bound to tRNA through its terminal carboxyl group, ...
... aminoacyl-tRNA (25, 26). For example, a poly U-30S ribosome subunit complex binds phenylalanyl-tRNA. A single codon, a triplet, is sufficient to direct specific binding of aminoacyl-tRNA to the ribosome (27). The growing polypeptide chain, which is bound to tRNA through its terminal carboxyl group, ...
The Mycobacterium tuberculosis katG promoter region contains a
... is in the correct orientation to promote expression of the luciferase gene. The 262 bp HindIII–SphI fragment of pK20 containing the katG upstream activator region (UAR) was cloned upstream of the PAN promoter in pLPan to form pLPuar. The structures of all the above constructs were confirmed by DNA s ...
... is in the correct orientation to promote expression of the luciferase gene. The 262 bp HindIII–SphI fragment of pK20 containing the katG upstream activator region (UAR) was cloned upstream of the PAN promoter in pLPan to form pLPuar. The structures of all the above constructs were confirmed by DNA s ...
Behavioral Objectives
... with your students. Health insurance companies are already beginning to deny or cancel insurance for women carrying the breast cancer gene. Is this ethical? Should other people insured through the same company pay higher rates to help cover the claims of women who have breast cancer treatment? Would ...
... with your students. Health insurance companies are already beginning to deny or cancel insurance for women carrying the breast cancer gene. Is this ethical? Should other people insured through the same company pay higher rates to help cover the claims of women who have breast cancer treatment? Would ...
CHAPTER 9
... interruption occurs by agitation of the solution in which the bacteria are found. This type of study is used to map the locations of genes. It is necessary to interrupt mating so that you can vary the time and obtain information about the order of transfer; which gene transferred first, second, and ...
... interruption occurs by agitation of the solution in which the bacteria are found. This type of study is used to map the locations of genes. It is necessary to interrupt mating so that you can vary the time and obtain information about the order of transfer; which gene transferred first, second, and ...
Visualizations of Microarray Data in Partek Genomics Suite 6.6
... signal for each genomic feature is shown on the horizontal axis (A), while the ratio is shown on the vertical axis (M). ...
... signal for each genomic feature is shown on the horizontal axis (A), while the ratio is shown on the vertical axis (M). ...
Fig 16.12a - McGraw Hill Higher Education
... Example: a2 repressor in yeast • Determines mating type by binding to enhancers of certain genes In a haploids and a/a diploids, a2 binds to enhancers of a-specific genes but not to enhancers of haploid-specific genes ...
... Example: a2 repressor in yeast • Determines mating type by binding to enhancers of certain genes In a haploids and a/a diploids, a2 binds to enhancers of a-specific genes but not to enhancers of haploid-specific genes ...
The Promises and Pitfalls of Genoeconomics
... genetic polymorphisms (from the Greek poly, meaning “many,” and morphisms, meaning “forms”). For simplicity, our discussion here focuses on the most common kind of genetic polymorphism, called a single-nucleotide polymorphism (SNP). SNPs are locations in the DNA sequence in which individuals differ ...
... genetic polymorphisms (from the Greek poly, meaning “many,” and morphisms, meaning “forms”). For simplicity, our discussion here focuses on the most common kind of genetic polymorphism, called a single-nucleotide polymorphism (SNP). SNPs are locations in the DNA sequence in which individuals differ ...
Benefits of being biased! - Indian Academy of Sciences
... by multiple codons. However, it is known that certain codons for a given amino acid are preferred over other synonymous codons. Theoretical studies have predicted and experiments have shown that this kind of a bias can lead to higher efficiency of translation, presumably accompanied by benefits in t ...
... by multiple codons. However, it is known that certain codons for a given amino acid are preferred over other synonymous codons. Theoretical studies have predicted and experiments have shown that this kind of a bias can lead to higher efficiency of translation, presumably accompanied by benefits in t ...
RNA-Seq

RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.