- California State University
... damage to the DNA during the amplification process and therefore allows researchers to observe the exact patterns of ancient DNA breakage due purely to degradation over time. This method also improves the recovery of long molecules, as well as the recovery of short molecules smaller than 30 base pai ...
... damage to the DNA during the amplification process and therefore allows researchers to observe the exact patterns of ancient DNA breakage due purely to degradation over time. This method also improves the recovery of long molecules, as well as the recovery of short molecules smaller than 30 base pai ...
Array-based sequencing of filaggrin gene for
... allelic variant between CNV repeats (Figure 2F). However, this particular variant was not informative for CNV identification in Caucasian samples we analysed (data not shown). Based on the allele frequency of the SNV together with coverage data we make a call regarding the presence of repeat 82. Fin ...
... allelic variant between CNV repeats (Figure 2F). However, this particular variant was not informative for CNV identification in Caucasian samples we analysed (data not shown). Based on the allele frequency of the SNV together with coverage data we make a call regarding the presence of repeat 82. Fin ...
No more than 14: the end of the amphioxus Hox cluster
... amphioxus cluster surprisingly showed the presence not only of 13 Hox genes, but a 14th Hox gene, AmphiHox14 [7]. The lack of a Hox14 gene in vertebrate Hox clusters and the faster rate of sequence divergence of the posterior class of Hox genes (so-called “posterior flexibility”) had made it difficu ...
... amphioxus cluster surprisingly showed the presence not only of 13 Hox genes, but a 14th Hox gene, AmphiHox14 [7]. The lack of a Hox14 gene in vertebrate Hox clusters and the faster rate of sequence divergence of the posterior class of Hox genes (so-called “posterior flexibility”) had made it difficu ...
p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA
... that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb in the human gene), which has been conserved through evolut ...
... that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb in the human gene), which has been conserved through evolut ...
PowerPoint
... integration and analysis of PID phenotype with the genotype data that are taken from RAPID. It currently holds 1438 PIDphenotype terms that are mapped and standardized using logic based assessment approach and represented in the form of Web Ontology Language (OWL) and Resource Description Framework ...
... integration and analysis of PID phenotype with the genotype data that are taken from RAPID. It currently holds 1438 PIDphenotype terms that are mapped and standardized using logic based assessment approach and represented in the form of Web Ontology Language (OWL) and Resource Description Framework ...
Adenylate Energy Charge
... Starvation of organisms. Bacteria were harvested late in the period of arithmetic growth and just before the onset of stationary phase. They were washed with 67 mM-KNa phosphate buffer, pH 6.8, and then resuspended in the same buffer at approx. 0.8 mg dry wt,"ml.The suspension was placed in a starva ...
... Starvation of organisms. Bacteria were harvested late in the period of arithmetic growth and just before the onset of stationary phase. They were washed with 67 mM-KNa phosphate buffer, pH 6.8, and then resuspended in the same buffer at approx. 0.8 mg dry wt,"ml.The suspension was placed in a starva ...
- Sankara Nethralaya
... nominal evidence of association and a shallower anterior chamber depth (ACD) when all data were meta-analyzed (β = −0.033, P = 0.021). However, when multiple testing was considered, the observation was non-significant.10 In another recent GWAS study from a larger Asian cohort ABCC5 was found to influe ...
... nominal evidence of association and a shallower anterior chamber depth (ACD) when all data were meta-analyzed (β = −0.033, P = 0.021). However, when multiple testing was considered, the observation was non-significant.10 In another recent GWAS study from a larger Asian cohort ABCC5 was found to influe ...
Epistemic goal as aspect of meaning
... Pragmatic factors are relevant for biological terms in two ways. First, a semantic approach such as two-dimensional semantics rightly acknowledges that in addition to reference a term has also an intension (construed truth-conditionally), but it ignores the fact that even scientific terms can exhibi ...
... Pragmatic factors are relevant for biological terms in two ways. First, a semantic approach such as two-dimensional semantics rightly acknowledges that in addition to reference a term has also an intension (construed truth-conditionally), but it ignores the fact that even scientific terms can exhibi ...
Archives of Microbiology 167:
... genomic cosmid library (Cohen et al. 1994). Nine cosmids hybridized when probed with the flanking DNA from pSCR210. Upon subsequent isolation and characterization, three cosmids showed similar restriction patterns in Southern hybridizations. We attempted to clone several larger genomic fragments (5. ...
... genomic cosmid library (Cohen et al. 1994). Nine cosmids hybridized when probed with the flanking DNA from pSCR210. Upon subsequent isolation and characterization, three cosmids showed similar restriction patterns in Southern hybridizations. We attempted to clone several larger genomic fragments (5. ...
Week 3. Gel electrophoresis and Bioinformatics
... laboratory by following the instructions provided. When you are finished with the laboratory, upload your completed handout to the course management system. Task 1: Prepare the samples The PCR reactions from last week have a total volume of 25 L. Pipette 10 L of this reaction into a clean 500 L t ...
... laboratory by following the instructions provided. When you are finished with the laboratory, upload your completed handout to the course management system. Task 1: Prepare the samples The PCR reactions from last week have a total volume of 25 L. Pipette 10 L of this reaction into a clean 500 L t ...
Efficient Ends-Out Gene Targeting In Drosophila
... pRK2 was generated by adding GMR enhancer into the BsiWI site of pRK1. In addition, enzyme sites in 5' MCS are ordered similarly to the popular pUAST vector. Thus, when pRK1 or pRK2 is used for making knock-in constructs, such as generating GFP fusion alleles of a target gene, the same GFP tagged 5' ...
... pRK2 was generated by adding GMR enhancer into the BsiWI site of pRK1. In addition, enzyme sites in 5' MCS are ordered similarly to the popular pUAST vector. Thus, when pRK1 or pRK2 is used for making knock-in constructs, such as generating GFP fusion alleles of a target gene, the same GFP tagged 5' ...
Drosophila Muller F Elements Maintain a Distinct Set of Genomic
... using a sliding window of 1 kb with a step size of 500 bp. Gene annotations This comparative analysis used the high-quality D. melanogaster gene annotations (release 5.50) produced by FlyBase as reference (Marygold et al. 2013). The annotation protocol has been described previously (Shaffer et al. 2 ...
... using a sliding window of 1 kb with a step size of 500 bp. Gene annotations This comparative analysis used the high-quality D. melanogaster gene annotations (release 5.50) produced by FlyBase as reference (Marygold et al. 2013). The annotation protocol has been described previously (Shaffer et al. 2 ...
Twinning and Embryonic Left-Right Asymmetry
... illustrates the expression pattern of three such genes as assayed by in situ hybridization with riboprobes to the relevant genes: Sonic Hedgehog (Shh), Nodal, and PTC (Levin et al., 1995). Shh is expressed only on the left side of Hensen’s node in the gastrulating chick embryo. Shortly thereafter, N ...
... illustrates the expression pattern of three such genes as assayed by in situ hybridization with riboprobes to the relevant genes: Sonic Hedgehog (Shh), Nodal, and PTC (Levin et al., 1995). Shh is expressed only on the left side of Hensen’s node in the gastrulating chick embryo. Shortly thereafter, N ...
Hereditary Inflammatory Breast Cancer Associated With BRCA2
... damage in BRCA-mutated breast cancers have done so in the previously treated metastatic setting.13-15 We do know that higher complete response (CR) rates have been achieved in ER-negative, BRCA1-mutated breast cancers treated in the neoadjuvant setting.16 Recently, it has also been shown that while ...
... damage in BRCA-mutated breast cancers have done so in the previously treated metastatic setting.13-15 We do know that higher complete response (CR) rates have been achieved in ER-negative, BRCA1-mutated breast cancers treated in the neoadjuvant setting.16 Recently, it has also been shown that while ...
Xiong, N., C.H. Kang, and D.H. Raulet. 2002. Redundant and unique roles of two enhancer elements in the TCR gamma locus in gene regulation and gamma delta T cell development. Immunity 16:453-463.
... separately has little effect. In contrast to these results in thymocytes, deletion of HsA alone reduces transcription of one V␥ gene specifically in peripheral ␥␦ T cells. Thus, the two elements exhibit functional redundancy in thymocytes but also have unique functions in other settings. Introductio ...
... separately has little effect. In contrast to these results in thymocytes, deletion of HsA alone reduces transcription of one V␥ gene specifically in peripheral ␥␦ T cells. Thus, the two elements exhibit functional redundancy in thymocytes but also have unique functions in other settings. Introductio ...
Evidence, Mechanisms and Models for the Inheritance of Acquired
... mammals, to be established and maintained. Since the fidelity with which functional states are transmitted can be very high, it is not surprising that there are many cases where a hereditary variation that was at first assumed to be a variation in DNA sequence, was later shown to be an epigenetic va ...
... mammals, to be established and maintained. Since the fidelity with which functional states are transmitted can be very high, it is not surprising that there are many cases where a hereditary variation that was at first assumed to be a variation in DNA sequence, was later shown to be an epigenetic va ...
Basic sequence analyses and submission
... R2) do not have vector because the primers were designed within the cloned segment. 5. Use BLAST 2 sequences to Align M13_F with F1 http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi (create a bookmark for this site) Copy the M13_F sequence in the Sequence 1 window and the F1 sequence in the Seque ...
... R2) do not have vector because the primers were designed within the cloned segment. 5. Use BLAST 2 sequences to Align M13_F with F1 http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi (create a bookmark for this site) Copy the M13_F sequence in the Sequence 1 window and the F1 sequence in the Seque ...
Nonrandom cell-cycle timing of a somatic chromosomal
... Xp11.2 that encodes a member of the microphthalmia-TFE subfamily of basic-helix-loop-helix leucine zipper transcription factors, to ASPSCR1 (also known as ASPL), a novel gene at 17q25 (Ladanyi et al., 2001). The resulting fusion gene encodes chimeric ASPSCR1–TFE3 RNA transcripts. In ASPS, this trans ...
... Xp11.2 that encodes a member of the microphthalmia-TFE subfamily of basic-helix-loop-helix leucine zipper transcription factors, to ASPSCR1 (also known as ASPL), a novel gene at 17q25 (Ladanyi et al., 2001). The resulting fusion gene encodes chimeric ASPSCR1–TFE3 RNA transcripts. In ASPS, this trans ...
Sex-specific Trans-regulatory Variation on the Drosophila melanogaster X Chromosome
... When the X chromosome stops recombining with the Y chromosome, a unique genomic environment is formed. Several important population genetic parameters are presumably affected, and collectively this sets the scene for different patterns of evolution on the X chromosome [1–3]. In particular, the level ...
... When the X chromosome stops recombining with the Y chromosome, a unique genomic environment is formed. Several important population genetic parameters are presumably affected, and collectively this sets the scene for different patterns of evolution on the X chromosome [1–3]. In particular, the level ...
Molecular Evidence for Vector Implication of Onchocerca lupi in Los
... The nodules are primarily composed of eosinophils, histiocytes, fibroblasts, and newly formed blood vessels (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). This is typically associated with exophthalmos and possible third-eyelid protrusion (Komnenou, Egyed, Sréter, & Eberhard, 2003). In almost all ...
... The nodules are primarily composed of eosinophils, histiocytes, fibroblasts, and newly formed blood vessels (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). This is typically associated with exophthalmos and possible third-eyelid protrusion (Komnenou, Egyed, Sréter, & Eberhard, 2003). In almost all ...
Rapid communication: Nucleotide sequence of red seabream
... Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan, Minato-ku, Tokyo 108-8477, Japan ...
... Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan, Minato-ku, Tokyo 108-8477, Japan ...
Genetic analysis of metabolites in apple fruits indicates an mQTL
... genetic basis of metabolic variability in apple fruits. This study was initiated without any a priori knowledge with regard to the specific metabolite groups. For that reason, large-scale liquid chromatography–mass spectrometry (LCMS)-based metabolic profiling was chosen. Metabolomics is defined as ...
... genetic basis of metabolic variability in apple fruits. This study was initiated without any a priori knowledge with regard to the specific metabolite groups. For that reason, large-scale liquid chromatography–mass spectrometry (LCMS)-based metabolic profiling was chosen. Metabolomics is defined as ...
General remarks: A biodesign is a man made, mostly computer
... customer’s requirements for the biological or other like technical functionality of the DNAs or its gene products of interest. The results for this assessment of the best mode for the biodesign of gene variants are presented to the customer in a detailed report and discussed before starting the real ...
... customer’s requirements for the biological or other like technical functionality of the DNAs or its gene products of interest. The results for this assessment of the best mode for the biodesign of gene variants are presented to the customer in a detailed report and discussed before starting the real ...
Supplementary Notes S1 (doc 64K)
... polymorphisms listed in the DGV (version- variation.hg18.v10.nov.2010) as described above. Primers were designed using Primer Express (Applied Biosystems) and purchased from Integrated DNA Technologies (www.idtdna.com) in lab ready format. The patient's DNA was diluted in PCR-grade water, and the qu ...
... polymorphisms listed in the DGV (version- variation.hg18.v10.nov.2010) as described above. Primers were designed using Primer Express (Applied Biosystems) and purchased from Integrated DNA Technologies (www.idtdna.com) in lab ready format. The patient's DNA was diluted in PCR-grade water, and the qu ...
Mitonuclear linkage disequilibrium in human populations
... Figure 1. Illustration of sign epistasis in mitonuclear interactions. The coloured organelles reflect the concept of matched and mismatched pairings between mitochondria and nuclei. associated with physical manipulation of reproductive cells, but an additional concern has emerged from the field of e ...
... Figure 1. Illustration of sign epistasis in mitonuclear interactions. The coloured organelles reflect the concept of matched and mismatched pairings between mitochondria and nuclei. associated with physical manipulation of reproductive cells, but an additional concern has emerged from the field of e ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.