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Ch.14 - Jamestown School District
Ch.14 - Jamestown School District

...  Cystic fibrosis is caused by the deletion of 3 bases in the DNA of a single gene – As a result, the body does not produce a protein needed to transport chloride ions ...
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... circle and move outward! • UCG = Serine • CAC = • GGU = • UAA =  There are 64 codons but only 20 amino acids. So, different codons can code for the same amino acid. ...
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Ch .15 - Crestwood Local Schools
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... based on triplets of bases. Has redundancy; some AA's have more than 1 code. Proof - make artificial RNA and see what AAs are used in protein synthesis (early 1960’s). ...
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... For the mini-TN-seq validation, olj376 was paired with HK178 in the first PCR reaction and HK179 was paired with an indexing primer in the second PCR reaction. The custom sequencing primer HK180 was used. ...
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MicroRNA: A novel class of master regulators of gene expression

... by destabilizing the mRNA. Since most target sites on the mRNA have only partial base complementarity with their corresponding microRNA, individual microRNAs may target as many as 100 different mRNAs. Additionally, individual mRNAs may contain multiple binding sites for different microRNA, leading t ...
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... that ALL humans are very similar in their DNA sequence - .01% Difference; 99.99% Identical. That is amazing!) Single Nucleotide Polymorphisms – this term refers to genes where a single nucleotide difference in sequence can affect the phenotypic outcome (For example the color of the actual protein pi ...
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... restriction enzymes (cut) and ligase (paste) DNA • Cloning DNA: E coli, lambda bacteriophage, yeast • Studying where and when mRNA is expressed: in situ hybridization, RT-PCR, Northern blots, Gene arrays • Transgenic mice • Homologous recombination ...
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... • The STE genes can be used to track the effects of mutations at other loci, such as MAT. • STE response be measured as fertility/sterility (mating). • Or, reporter gene constructs made with the transcriptional response elements from STE genes can drive the E. coli -galactosidase gene. • The report ...
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... Distribution of the gene regulatory proteins responsible for ensuring that eve is expressed in stripe 2. The distributions of these proteins were visualized by staining a developing Drosophila embryo with antibodies directed against each of the four proteins The expression of eve in stripe 2 occurs ...
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... RNA and protein construction • The nucleotide base sequence of mRNA is encoded from DNA and transmits sequences of bases used to determine the amino acid sequence of the protein. • mRNA (“Messenger RNA”) associates with the ribosome (mRNA and protein portion). • RNA (“Transfer RNA”) also required • ...
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Abell Endowment Distinguished Lecture in Computer Engineering

... Since 1995, large-scale gene discovery and mapping focused on disease gene mutation discovery and understanding, have been at the heart of research efforts in the CLCG and CBCB at the University of Iowa. Together with faculty in the UI Carver College of Medicine, advanced computational, mathematical ...
PSYC 200 Chapter 3
PSYC 200 Chapter 3

... What Genes Are Allele • A variation that makes a gene different in some way from other genes for the same characteristics • Many genes never vary; others have several possible alleles ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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