OMPROT PP2 ver4 - Plant Physiology
... This membrane fractionation was performed on two samples, the first being FFE enriched mitochondria to yield enriched mitochondrial outer membrane (Mt OM), the second being the crude organelle enrichment HSP to yield a contaminated outer membrane fraction (HSPOM). Additional pre-fractionated samples ...
... This membrane fractionation was performed on two samples, the first being FFE enriched mitochondria to yield enriched mitochondrial outer membrane (Mt OM), the second being the crude organelle enrichment HSP to yield a contaminated outer membrane fraction (HSPOM). Additional pre-fractionated samples ...
Distinct C-terminal Amino Acid Sequence Motifs Serve
... Tail-anchored (TA) proteins are a unique class of functionally diverse membrane proteins that are defined by their single C-terminal transmembrane domain and their ability to insert post-translationally into specific organelles in an Ncytosol-CIMS orientation. While in recent years considerable prog ...
... Tail-anchored (TA) proteins are a unique class of functionally diverse membrane proteins that are defined by their single C-terminal transmembrane domain and their ability to insert post-translationally into specific organelles in an Ncytosol-CIMS orientation. While in recent years considerable prog ...
The novel cytochrome c6 of chloroplasts
... databases) and conserved indicated that it encoded a functional protein. Remarkably, all the predicted amino acid sequences for the plant protein contained a conserved insertion of 12 amino acids compared with the bacterial and algal proteins. This insertion occurs in a region corresponding to a loo ...
... databases) and conserved indicated that it encoded a functional protein. Remarkably, all the predicted amino acid sequences for the plant protein contained a conserved insertion of 12 amino acids compared with the bacterial and algal proteins. This insertion occurs in a region corresponding to a loo ...
Autophagy Contributes to Leaf Starch DegradationC
... in the central vacuole of mesophyll cells after 4 h of exposure to darkness, but fewer were visible at other time points. The red asterisk indicates a mitochondrion that has entered the vacuole. Cp, chloroplast; M, mitochondrion; S, starch; V, vacuole. Bars = 2.5 mm. (B) Representative ultrastructur ...
... in the central vacuole of mesophyll cells after 4 h of exposure to darkness, but fewer were visible at other time points. The red asterisk indicates a mitochondrion that has entered the vacuole. Cp, chloroplast; M, mitochondrion; S, starch; V, vacuole. Bars = 2.5 mm. (B) Representative ultrastructur ...
Autophagy Contributes to Leaf Starch DegradationC
... in the central vacuole of mesophyll cells after 4 h of exposure to darkness, but fewer were visible at other time points. The red asterisk indicates a mitochondrion that has entered the vacuole. Cp, chloroplast; M, mitochondrion; S, starch; V, vacuole. Bars = 2.5 mm. (B) Representative ultrastructur ...
... in the central vacuole of mesophyll cells after 4 h of exposure to darkness, but fewer were visible at other time points. The red asterisk indicates a mitochondrion that has entered the vacuole. Cp, chloroplast; M, mitochondrion; S, starch; V, vacuole. Bars = 2.5 mm. (B) Representative ultrastructur ...
Autophagy Contributes to Leaf Starch DegradationC
... in the central vacuole of mesophyll cells after 4 h of exposure to darkness, but fewer were visible at other time points. The red asterisk indicates a mitochondrion that has entered the vacuole. Cp, chloroplast; M, mitochondrion; S, starch; V, vacuole. Bars = 2.5 mm. (B) Representative ultrastructur ...
... in the central vacuole of mesophyll cells after 4 h of exposure to darkness, but fewer were visible at other time points. The red asterisk indicates a mitochondrion that has entered the vacuole. Cp, chloroplast; M, mitochondrion; S, starch; V, vacuole. Bars = 2.5 mm. (B) Representative ultrastructur ...
Presence of multiple group I introns closely 23S rRNAs of lichen-forming
... Relationship between introns at positions L1917, L1931 and L1951 with bacterial group I introns. With respect to host evolution, an examination of the LSU rDNA tree (Fig. 1B) shows that the members of the Trebouxia genus analyzed in this study form two distinct main lineages: lineage 1, defined by T ...
... Relationship between introns at positions L1917, L1931 and L1951 with bacterial group I introns. With respect to host evolution, an examination of the LSU rDNA tree (Fig. 1B) shows that the members of the Trebouxia genus analyzed in this study form two distinct main lineages: lineage 1, defined by T ...
curriculum vitae- michael hippler
... reinhardtii in aerobic and anaerobic (induced by 8 hours of argon bubbling) conditions were isolated and analyzed using comparative proteomics. A total of 2315 proteins were identified. Further analysis based on spectral counting localized 895 of these proteins to the chloroplast, including many pro ...
... reinhardtii in aerobic and anaerobic (induced by 8 hours of argon bubbling) conditions were isolated and analyzed using comparative proteomics. A total of 2315 proteins were identified. Further analysis based on spectral counting localized 895 of these proteins to the chloroplast, including many pro ...
Multiple Lines of Evidence Localize Signaling
... Using GFP tagging, mitochondria have been visualized to undergo fusion, fission, and rapid movements, suggesting a dynamic interaction with components of the cytoskeleton (Sheahan et al., 2004, 2005; Logan, 2010). However, many of the specific proteins that mediate such processes remain unknown. Whi ...
... Using GFP tagging, mitochondria have been visualized to undergo fusion, fission, and rapid movements, suggesting a dynamic interaction with components of the cytoskeleton (Sheahan et al., 2004, 2005; Logan, 2010). However, many of the specific proteins that mediate such processes remain unknown. Whi ...
Functional Characterisation of Plant
... outer membrane proteome between plants and other groups of organisms. Whilst the mitochondrial outer membrane proteome has been studied in model organisms such as Saccharomyces cerevisiae or Neurospora crassa, the components present in Arabidopsis thaliana (Arabidopsis) have been mostly deduced from ...
... outer membrane proteome between plants and other groups of organisms. Whilst the mitochondrial outer membrane proteome has been studied in model organisms such as Saccharomyces cerevisiae or Neurospora crassa, the components present in Arabidopsis thaliana (Arabidopsis) have been mostly deduced from ...
In vivo quantification of peroxisome tethering to chloroplasts in
... throughout the plant life cycle (Hu et al. 2012), then an understanding of if and how ...
... throughout the plant life cycle (Hu et al. 2012), then an understanding of if and how ...
Identification of a Novel Gene, CIA6, Required for
... Chlamydomonas reinhardtii possesses a CO2-concentrating mechanism (CCM) that allows the alga to grow at low CO2 concentrations. One common feature seen in photosynthetic organisms possessing a CCM is the tight packaging of Rubisco within the cell. In many eukaryotic algae, Rubisco is localized to th ...
... Chlamydomonas reinhardtii possesses a CO2-concentrating mechanism (CCM) that allows the alga to grow at low CO2 concentrations. One common feature seen in photosynthetic organisms possessing a CCM is the tight packaging of Rubisco within the cell. In many eukaryotic algae, Rubisco is localized to th ...
Mitochondria - Physical Mathematics
... most striking features of both mitochondria and chloroplasts are their extensive internal membrane systems. These internal membranes contain sets of membrane protein complexes that work together to produce most of the cell’s ATP. In bacteria, simpler versions of essentially the same protein complexe ...
... most striking features of both mitochondria and chloroplasts are their extensive internal membrane systems. These internal membranes contain sets of membrane protein complexes that work together to produce most of the cell’s ATP. In bacteria, simpler versions of essentially the same protein complexe ...
The Role of the Bundle Sheath in the Leaf Development of C3 plants
... (CAB) gene underexpressed1 (cue1) (Streatfield et al., 1999), differential development of vasculature associated cells1 (dov1) (Kinsman and Pyke, 1998), and venosa3 and 6 (ven3 and ven6) (Mollá-Morales et al., 2011). By the start of the project, only the function of cue1 was identified. Cue1 is defe ...
... (CAB) gene underexpressed1 (cue1) (Streatfield et al., 1999), differential development of vasculature associated cells1 (dov1) (Kinsman and Pyke, 1998), and venosa3 and 6 (ven3 and ven6) (Mollá-Morales et al., 2011). By the start of the project, only the function of cue1 was identified. Cue1 is defe ...
Proteome analysis of tobacco BY-2 cell culture - ETH E
... Plastids, essential cell organelles, are present in all living cells of plants, except pollen. They are responsible for many of the essential biosynthetic and metabolic activities required for the basic architecture and functions of plant cells. Depending on the tissue type, they are differentiated ...
... Plastids, essential cell organelles, are present in all living cells of plants, except pollen. They are responsible for many of the essential biosynthetic and metabolic activities required for the basic architecture and functions of plant cells. Depending on the tissue type, they are differentiated ...
Evidence for chloroplast control of external Ca -induced
... Recently, molecular genetic studies have suggested that chloroplasts may be involved in the generation of cytosolic Ca2+ signals in plant cells. A putative thylakoid membranelocalized Ca2+ carrier protein, PPF1, may regulate flowering time by modulating the Ca2+ storage capacity of chloroplasts (Li ...
... Recently, molecular genetic studies have suggested that chloroplasts may be involved in the generation of cytosolic Ca2+ signals in plant cells. A putative thylakoid membranelocalized Ca2+ carrier protein, PPF1, may regulate flowering time by modulating the Ca2+ storage capacity of chloroplasts (Li ...
A re-evaluation of the ATP :NADPH budget
... chloroplastic GS/GOGAT system, is not shown (this process will involve utilization of photosynthetic electrons and ATP and is included in the calculations of Tables 1 and 2). Unless stated otherwise, the calculations of Tables 1 and 2 assume that hydroxypyruvate reduction is NADHdependent and is per ...
... chloroplastic GS/GOGAT system, is not shown (this process will involve utilization of photosynthetic electrons and ATP and is included in the calculations of Tables 1 and 2). Unless stated otherwise, the calculations of Tables 1 and 2 assume that hydroxypyruvate reduction is NADHdependent and is per ...
A Personal Account of Chaperonin History
... short there was too much Rubisco in the pot. So Pierre was sent back to the bench with instructions to use equimolar amounts of unfolded Rubisco and GroEL 14-mers. Wow! Now we recovered ⬎80%. It was almost too good to be true and so the very next day I repeated (successfully) the experiment with my ...
... short there was too much Rubisco in the pot. So Pierre was sent back to the bench with instructions to use equimolar amounts of unfolded Rubisco and GroEL 14-mers. Wow! Now we recovered ⬎80%. It was almost too good to be true and so the very next day I repeated (successfully) the experiment with my ...
The metabolism of photosynthetically fixed
... under the conditions of these experiments are of the right order to account for the observed weight differences. This paper describes a preliminary study of how the animal metabolizes photosynthetically fixed carbon released from its “symbiotic” chloroplasts. Previous experimental investigations of ...
... under the conditions of these experiments are of the right order to account for the observed weight differences. This paper describes a preliminary study of how the animal metabolizes photosynthetically fixed carbon released from its “symbiotic” chloroplasts. Previous experimental investigations of ...
Electron microscopy in structural studies of Photosystem II
... are termed stroma lamellae whereas the stacked regions form grana membranes. Unstacked regions are termed stroma lamellae whereas stacked regions form grana membranes. Grana membranes are approximately circular with a diameter of about 500 nm and are in close contact with two thylakoid vesicles, as ...
... are termed stroma lamellae whereas the stacked regions form grana membranes. Unstacked regions are termed stroma lamellae whereas stacked regions form grana membranes. Grana membranes are approximately circular with a diameter of about 500 nm and are in close contact with two thylakoid vesicles, as ...
Intraplastidic Localization of the Enzymesthat Convert Cucumber
... Laboratory of Plant Pigment Biochemistry and Photobiology, 202 ABL, University of Illinois, Urbana, Illinois 61801 these enzymes is more controversial. On the basis of osmotic lysis of crude etiochloroplast preparations accompanied by differential centrifugation, Smith and Rebeiz (27) proposed that ...
... Laboratory of Plant Pigment Biochemistry and Photobiology, 202 ABL, University of Illinois, Urbana, Illinois 61801 these enzymes is more controversial. On the basis of osmotic lysis of crude etiochloroplast preparations accompanied by differential centrifugation, Smith and Rebeiz (27) proposed that ...
Principles of redox control in photosynthesis gene
... components during chlororespiration. In the dark, it can be reduced by reduction equivalents via the NDH complex (Feild et al. 1998, Kofer et al. 1998). In addition, it was found that PQ can act as a electron carrier between thylakoid NADH dehydrogenase and peroxidase (Casano et al. 2000). Therefor ...
... components during chlororespiration. In the dark, it can be reduced by reduction equivalents via the NDH complex (Feild et al. 1998, Kofer et al. 1998). In addition, it was found that PQ can act as a electron carrier between thylakoid NADH dehydrogenase and peroxidase (Casano et al. 2000). Therefor ...
The End of the Line: Can Ferredoxin and Ferredoxin NADP(H
... the light harvesting complex of PSII can generate the extremely dangerous singlet oxygen radical [18]. Higher plants and algae prevent this through a variety of mechanisms referred to as non-photochemical quenching (NPQ) [19]. In this case, activity of PSII is down regulated through a combination of ...
... the light harvesting complex of PSII can generate the extremely dangerous singlet oxygen radical [18]. Higher plants and algae prevent this through a variety of mechanisms referred to as non-photochemical quenching (NPQ) [19]. In this case, activity of PSII is down regulated through a combination of ...
ATP-dependent Clp Proteases in Photosynthetic Organisms~ A Cut
... the best characterized proteases to date, and were the first shown to rely on an ATPase regulatory subunit possessing molecular chaperone activity, which functions both within the proteolytic complex and independently. A range of Clp proteins has been identified from many different bacteria and euka ...
... the best characterized proteases to date, and were the first shown to rely on an ATPase regulatory subunit possessing molecular chaperone activity, which functions both within the proteolytic complex and independently. A range of Clp proteins has been identified from many different bacteria and euka ...
Focus Issue on Plastid Biology Update Novel
... inactivation of target proteins e.g. in the Calvin cycle, is unrelated to the value of Em but highly relevant for photoinhibition under non-optimal environmental conditions such as chilling temperatures (Hutchinson et al. 2000). This complexity is partly explained by the fact that thiol modulation i ...
... inactivation of target proteins e.g. in the Calvin cycle, is unrelated to the value of Em but highly relevant for photoinhibition under non-optimal environmental conditions such as chilling temperatures (Hutchinson et al. 2000). This complexity is partly explained by the fact that thiol modulation i ...