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Question 2 (20 points)
Question 2 (20 points)

Rapid enzyme assays investigating the variation in the glycolytic
Rapid enzyme assays investigating the variation in the glycolytic

... enzyme and how many enzymes exhibit significant variation in expression (i.e., how prevalent this variation is). To investigate these two questions we choose a system where temperature varies across a species' distribution in a consistent manner, because of the influence environmental temperature ha ...
ERT320 BIOSEPARATION ENGINEERING
ERT320 BIOSEPARATION ENGINEERING

Document
Document

... Remember, aerobic prokaryotes (bacteria) do not have mitochondria, but they do have cellular respiration = Kreb’s cycle and oxidative phosphorylation (electron-transport chain). ...
Self-Organizing Bio
Self-Organizing Bio

... Nature has “chosen” a path that leads to further developments/evolution (according to the laws n Physics and Chemistry) ...
Gelatinization of Starch
Gelatinization of Starch

... Both enzymes have trouble with α (1 - 6) linkages ...
Biomolecule Discussion Guide KEY
Biomolecule Discussion Guide KEY

... and fatty acids or glycerol and fatty acids plus a phosphate group. They are insoluble in water, and do not form large polymers—just two or three fatty acids with glycerol (diglyceride and triglyceride). b. Function: Energy storage, insulation, part of cell membrane, hormones ©2012, TESCCC ...
Judgement Statement – 2012
Judgement Statement – 2012

... Explains how transcription is affected by deletion in the myostatin gene. • The loss of 11 base pairs / deletion mutation means the DNA base sequence / triplets is changed / causes a reading frameshift, so that the nucleotides that make the RNA (codon) are now in a different sequence / codons the RN ...
Part 1B: Understanding Biochemical Testing for Bacterial
Part 1B: Understanding Biochemical Testing for Bacterial

... source of carbohydrate but in order to do this, they must first hydrolyze or break down the starch so it may enter the cell. ...
Schedule
Schedule

... Then only P allele will convert / make to purple / pigment. In this case for all purple seeds to be produced by a white-seeded and red-seeded plant, there must have been two dominant alleles present for each of C and P genes. This is an example of a gene interaction (epistasis / supplementary) where ...
ppt
ppt

PPT
PPT

... There are about 20 amino acids. The ones known as "essential" amino acids must be found in the diet because the human body cannot produce them. There are about 10 essential amino acids. There are many references for these amino acids and they do not all agree. Some sources say there are only nine an ...
Q.No Genetic engineering is the technique of introducing foreign
Q.No Genetic engineering is the technique of introducing foreign

... the ability to dissolve most of the compounds in it. This is possible because of the polarity which water has. The molecule of the water comprise of two 10 elements hydrogen and oxygen. ...
enzymes-inhibition-text
enzymes-inhibition-text

... with the catalysis (e.g. by reacting with side-chains important for the catalysis); ...
Previous lecture: Today:
Previous lecture: Today:

... promote the reactivity of the enzyme’s groups through electrostatic effects Examples include metalloenzymes (urease)-important for maintaining proper structure of protein and active site residues Also enzymes that accept co-factors in metal form (kinases) MgATP (allows ATP to bind as neutral molecul ...
Prof. Kamakaka`s Lecture 5 Notes
Prof. Kamakaka`s Lecture 5 Notes

Proteins
Proteins

... THREE fatty acids. (Fig 5-10 pg 65). When created, it is called triacylglycerol, or triglyceride. Phospholipids are formed from TWO fatty acids and a glycerol. They are usually the skeleton of a cell membrane ...
Biochemistry PowerPoint
Biochemistry PowerPoint

... Each individual is unique because of their proteins which is determined by your DNA ...
Cyclic AMP and Hormone Action
Cyclic AMP and Hormone Action

... that work through the action of 3’-5’-cyclic AMP (cAMP) control enzymes by using ATP to phosphorylate serine and threonine groups on target enzymes. These so-called protein kinases represent a sequel of catalytic steps designed to amplify the action of the hormone. The key word here is “catalytic”, ...
Valine Mydrogenase from Streptmzyces fiadipe
Valine Mydrogenase from Streptmzyces fiadipe

... K,HPO.,, 0.1;L-valine, 0.47; yeast extract, 0.05. Both media were adjusted to pH 7.3 with 1 M-HClbefore thermal sterilization. Cultivation was done in 500 ml flasks containing 70 ml medium on a reciprocal shaker (1.7 Hz, 29 "C). Enzyme and protein assay. The ammonium-assimilating activity of VDH was ...
Review Problems #2 (Enzyme Review, Phosphatases
Review Problems #2 (Enzyme Review, Phosphatases

... structure of chorismate? What are the three immediate products derived from chorismate that constitute the first unique steps in the synthesis of the three aromatic amino acids? 3) From where are the two carbons of the five-membered ring of tryptophan derived? Be specific. (Show the molecule and hig ...
Solutions - MIT OpenCourseWare
Solutions - MIT OpenCourseWare

... b) Which of these reactions, 1, 2, or 3 is most likely to proceed in the forward direction in the absence of an enzyme? If you do not have enough information to answer this question, write “Can’t tell” below. Explain your answer. Reaction 1 is most likely to proceed in the forward direction in the a ...
Nutrients are chemical substances in food that provide energy, form
Nutrients are chemical substances in food that provide energy, form

... Some enzymes consist only of proteins. Most enzymes, however, contain a protein called an apoenzyme that is inactive without a nonprotein component called the cofactor. Together the apoenzyme and cofactor are an activated holoenzyme, or whole enzyme. If the cofactor is removed, the apoenzyme will no ...
2007
2007

... showed that the production of CO2 by the extract increased when succinate was added. In fact, for every mole of succinate added, many extra moles of CO2 were produced. Explain this effect in terms of the known catabolic pathways. ...
Endocrine System: Overview
Endocrine System: Overview

... Most physiological systems are located in specific locations in the body. The endocrine system does not fit this description. Explain why not. ...
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Enzyme



Enzymes /ˈɛnzaɪmz/ are macromolecular biological catalysts. Enzymes accelerate, or catalyze, chemical reactions. The molecules at the beginning of the process are called substrates and the enzyme converts these into different molecules, called products. Almost all metabolic processes in the cell need enzymes in order to occur at rates fast enough to sustain life. The set of enzymes made in a cell determines which metabolic pathways occur in that cell. The study of enzymes is called enzymology.Enzymes are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Enzymes' specificity comes from their unique three-dimensional structures.Like all catalysts, enzymes increase the rate of a reaction by lowering its activation energy. Some enzymes can make their conversion of substrate to product occur many millions of times faster. An extreme example is orotidine 5'-phosphate decarboxylase, which allows a reaction that would otherwise take millions of years to occur in milliseconds. Chemically, enzymes are like any catalyst and are not consumed in chemical reactions, nor do they alter the equilibrium of a reaction. Enzymes differ from most other catalysts by being much more specific. Enzyme activity can be affected by other molecules: inhibitors are molecules that decrease enzyme activity, and activators are molecules that increase activity. Many drugs and poisons are enzyme inhibitors. An enzyme's activity decreases markedly outside its optimal temperature and pH.Some enzymes are used commercially, for example, in the synthesis of antibiotics. Some household products use enzymes to speed up chemical reactions: enzymes in biological washing powders break down protein, starch or fat stains on clothes, and enzymes in meat tenderizer break down proteins into smaller molecules, making the meat easier to chew.
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