Enzyme Mechanisms
... Subtilisin: externals very different from mammalian serine proteases; triad same ...
... Subtilisin: externals very different from mammalian serine proteases; triad same ...
Protein Chemistry
... Secondary structure: This level is only concerned with the local or close in structures on the protein - peptide backbone. The side chains are not considered here, even though they have an affect on the secondary structure. Two common secondary structures - alpha helix and beta pleated sheet Non- ...
... Secondary structure: This level is only concerned with the local or close in structures on the protein - peptide backbone. The side chains are not considered here, even though they have an affect on the secondary structure. Two common secondary structures - alpha helix and beta pleated sheet Non- ...
Practice AP Multiple Choice Exam 1 Do NOT write on this! 1. Which
... 86) Reactants capable of interacting to form products in a chemical reaction must first overcome a thermodynamic barrier known as the reaction's A) entropy. B) activation energy. C) endothermic level. D) heat content. E) free-energy content. 87) Which of the following statements regarding enzymes is ...
... 86) Reactants capable of interacting to form products in a chemical reaction must first overcome a thermodynamic barrier known as the reaction's A) entropy. B) activation energy. C) endothermic level. D) heat content. E) free-energy content. 87) Which of the following statements regarding enzymes is ...
1 - Free
... 1. what is the most biological important function og HSP 70? 2. draw the double reciprocal plot of an uncompetitive inhibition for a one substrate enzyme reaction, inhibited by an inhibitor at two different concentrations. 3. from what compound and where is oxygen produced? 4. write two possibiliti ...
... 1. what is the most biological important function og HSP 70? 2. draw the double reciprocal plot of an uncompetitive inhibition for a one substrate enzyme reaction, inhibited by an inhibitor at two different concentrations. 3. from what compound and where is oxygen produced? 4. write two possibiliti ...
Hemophilia - Genomics Help
... “Promoter/Upstream by 1000 bases” and uncheck the box for “Introns” (this will remove all introns from the sequence that is retrieved). We also want to add 500 bases past the end of the gene, so check the box for “Downstream by 100 bases.” Also, make sure that under “Sequence Formatting Options,” th ...
... “Promoter/Upstream by 1000 bases” and uncheck the box for “Introns” (this will remove all introns from the sequence that is retrieved). We also want to add 500 bases past the end of the gene, so check the box for “Downstream by 100 bases.” Also, make sure that under “Sequence Formatting Options,” th ...
OMNI kit - EnzyPep
... A pH between pH 8.0 - 8.5 is optimal and required. At lower pH, the amine fragment is protonated, and thus unreactive; at higher pH, the Cam-ester is chemically hydrolyzed. Because peptides are usually acidic after TFA (trifluoroacetic acid) cleavage and a preparative HPLC step, it is critical to ch ...
... A pH between pH 8.0 - 8.5 is optimal and required. At lower pH, the amine fragment is protonated, and thus unreactive; at higher pH, the Cam-ester is chemically hydrolyzed. Because peptides are usually acidic after TFA (trifluoroacetic acid) cleavage and a preparative HPLC step, it is critical to ch ...
Chapter 3: Energy, Catalysis, and Biosynthesis
... provides a means of direct exchange of material goods. For example, the owner of a cow may have excess milk and need eggs, whereas a chicken owner has excess eggs and needs milk. Provided that these two people are in close proximity and can communicate, they may exchange or barter eggs for milk. But ...
... provides a means of direct exchange of material goods. For example, the owner of a cow may have excess milk and need eggs, whereas a chicken owner has excess eggs and needs milk. Provided that these two people are in close proximity and can communicate, they may exchange or barter eggs for milk. But ...
Topic 2 Review
... substrate concentration on enzyme activity • 2.3.4 – Define denaturation • 2.3.5 – Explain the use of pectinase in fruit juice production and one other commercial application of enzymes ...
... substrate concentration on enzyme activity • 2.3.4 – Define denaturation • 2.3.5 – Explain the use of pectinase in fruit juice production and one other commercial application of enzymes ...
Synthetic Consordium for Cellulose Hydrolysis and Ethanol Production
... Endoglucanase, Exoglucanase, β-glucosidase, and the scaffolding to hold all three enzymes together. The three cellulase-secreting yeast secrete all the enzymes made out into the common medium while the scaffolding latches on to the cell membrane as a surface display glycoprotein. To create the four ...
... Endoglucanase, Exoglucanase, β-glucosidase, and the scaffolding to hold all three enzymes together. The three cellulase-secreting yeast secrete all the enzymes made out into the common medium while the scaffolding latches on to the cell membrane as a surface display glycoprotein. To create the four ...
Origin of Life - stephen fleenor
... into polymers (DNA, RNA, proteins, and lipids) These molecules began self-replicating in water ...
... into polymers (DNA, RNA, proteins, and lipids) These molecules began self-replicating in water ...
Document
... • Raising gene dose simply increase the product, from products involving the activity of one or a few genes, such as enzymes. • This may be beneficial if the fermentation product is cell biomass or a primary metabolite. ...
... • Raising gene dose simply increase the product, from products involving the activity of one or a few genes, such as enzymes. • This may be beneficial if the fermentation product is cell biomass or a primary metabolite. ...
IB Topics DNA HL no writing
... consists of initiation, elongation and termination; mRNA translated in a 5' to 3' direction; binding of ribosome to mRNA; small sub-unit then large; first / initiator tRNA binds to start codon / to small subunit of ribosome; AUG is the start codon; second tRNA binds to ribosome; large subunit moves ...
... consists of initiation, elongation and termination; mRNA translated in a 5' to 3' direction; binding of ribosome to mRNA; small sub-unit then large; first / initiator tRNA binds to start codon / to small subunit of ribosome; AUG is the start codon; second tRNA binds to ribosome; large subunit moves ...
清华大学本科生考试试题专用纸
... Answer: The rate of triacylglycerol biosynthesis is affected by the action of several hormones, one of which is insulin. Insulin promotes the conversion of carbohydrates to triacylglycerols. People with severe diabetes, due to failure of insulin secretion or action, not only are unable to use glucos ...
... Answer: The rate of triacylglycerol biosynthesis is affected by the action of several hormones, one of which is insulin. Insulin promotes the conversion of carbohydrates to triacylglycerols. People with severe diabetes, due to failure of insulin secretion or action, not only are unable to use glucos ...
ch-3-bio-molecules
... Organic molecules are important because they are general types of molecules that all living organisms synthesize and use; they are essential for life ...
... Organic molecules are important because they are general types of molecules that all living organisms synthesize and use; they are essential for life ...
... supplied by a 15-watt Sylvania soft-white fluorescent tube. One milliliter of the treated conidial suspension was placed in each of twenty petri dishes and mixed with Neurqwra minimal ogar (Difco) supplemented with sorbore (8 sm./l .) to induce colony formotion. The plates were incubated at 2PC for ...
Crystal Structure of Octaprenyl Pyrophosphate Synthase from
... farnesyl pyrophosphate (FPP) with isopentenyl pyrophosphate (IPP) to generate C 40 octaprenyl pyrophosphate (OPP) which constitutes the side chain of bacterial ubiquinone or menaquinone. In this study, the first structure of long-chain C40-OPPs from Thermotoga maritima has been determined to 2.28 Å ...
... farnesyl pyrophosphate (FPP) with isopentenyl pyrophosphate (IPP) to generate C 40 octaprenyl pyrophosphate (OPP) which constitutes the side chain of bacterial ubiquinone or menaquinone. In this study, the first structure of long-chain C40-OPPs from Thermotoga maritima has been determined to 2.28 Å ...
DNA Restriction and Gel Electrophoresis This laboratory
... field. The molecule will migrate through the gel when the electric field is applied. We can use different types of gels that have different pore sizes. In this way, we can choose a gel that will separate DNA (or RNA or protein) fragments of a particular size best. The two types of gels most commonly ...
... field. The molecule will migrate through the gel when the electric field is applied. We can use different types of gels that have different pore sizes. In this way, we can choose a gel that will separate DNA (or RNA or protein) fragments of a particular size best. The two types of gels most commonly ...
Homology among (βα) 8 Barrels: Implications for the Evolution of
... SCOP, as the phosphate-binding site is occupied by IMP rather than by the NADP characteristic of other superfamily members. The recently solved structure of orotidine 50 -monophosphate decarboxylase is detected by our PSI-blast searches, and shares a similar phosphate-binding site (Appleby et al., 2 ...
... SCOP, as the phosphate-binding site is occupied by IMP rather than by the NADP characteristic of other superfamily members. The recently solved structure of orotidine 50 -monophosphate decarboxylase is detected by our PSI-blast searches, and shares a similar phosphate-binding site (Appleby et al., 2 ...
Islamic University of Gaza Advanced Biochemistry Faculty of
... C. The citrate synthase reaction is strongly exergonic. Why is this essential to maintaining flux through the TCA cycle? (2 points) Answer: The first reaction of the cycle is condensation of the methyl carbon of acetyl-CoA with the keto carbon (C-2) of oxaloacetate. The standard free energy of the r ...
... C. The citrate synthase reaction is strongly exergonic. Why is this essential to maintaining flux through the TCA cycle? (2 points) Answer: The first reaction of the cycle is condensation of the methyl carbon of acetyl-CoA with the keto carbon (C-2) of oxaloacetate. The standard free energy of the r ...
Antioxidant Enzymes and Function
... GSH = reduced glutathione; γ - Glu-Cys-Gly, γ- glutamylcysteinylglycine, GSSG = oxidized Rate limiting enzyme of pentose phosphate cycle is G-6-P dehydrogenase NADP & GSSG both overcome NADPH inhibition of G-6-P dehydrogenase BSO - buthionine sulfoximine inhibits GSH synthesis ...
... GSH = reduced glutathione; γ - Glu-Cys-Gly, γ- glutamylcysteinylglycine, GSSG = oxidized Rate limiting enzyme of pentose phosphate cycle is G-6-P dehydrogenase NADP & GSSG both overcome NADPH inhibition of G-6-P dehydrogenase BSO - buthionine sulfoximine inhibits GSH synthesis ...
bioCHEMISTRY 480 Molecular Biochemistry-‐
... keys to Spring 2015 tests, quizzes, homework, (5) Biochemistry web sites, (6) Bioinformatics CourseApproach: This course will study the chemical principles of structure and interactions of chemical species th ...
... keys to Spring 2015 tests, quizzes, homework, (5) Biochemistry web sites, (6) Bioinformatics CourseApproach: This course will study the chemical principles of structure and interactions of chemical species th ...
Practice Exam #1
... 3. ADP, Pi and H+ are substrates for ATP production in the mitochondria. 4. Pi is an allosteric activator of both phosphorylase and phosphofructokinase. 5. The low concentrations of substrates and products inside a cell make enzymes essential for meaningful product formation and the regulation of me ...
... 3. ADP, Pi and H+ are substrates for ATP production in the mitochondria. 4. Pi is an allosteric activator of both phosphorylase and phosphofructokinase. 5. The low concentrations of substrates and products inside a cell make enzymes essential for meaningful product formation and the regulation of me ...
Enzyme
Enzymes /ˈɛnzaɪmz/ are macromolecular biological catalysts. Enzymes accelerate, or catalyze, chemical reactions. The molecules at the beginning of the process are called substrates and the enzyme converts these into different molecules, called products. Almost all metabolic processes in the cell need enzymes in order to occur at rates fast enough to sustain life. The set of enzymes made in a cell determines which metabolic pathways occur in that cell. The study of enzymes is called enzymology.Enzymes are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Enzymes' specificity comes from their unique three-dimensional structures.Like all catalysts, enzymes increase the rate of a reaction by lowering its activation energy. Some enzymes can make their conversion of substrate to product occur many millions of times faster. An extreme example is orotidine 5'-phosphate decarboxylase, which allows a reaction that would otherwise take millions of years to occur in milliseconds. Chemically, enzymes are like any catalyst and are not consumed in chemical reactions, nor do they alter the equilibrium of a reaction. Enzymes differ from most other catalysts by being much more specific. Enzyme activity can be affected by other molecules: inhibitors are molecules that decrease enzyme activity, and activators are molecules that increase activity. Many drugs and poisons are enzyme inhibitors. An enzyme's activity decreases markedly outside its optimal temperature and pH.Some enzymes are used commercially, for example, in the synthesis of antibiotics. Some household products use enzymes to speed up chemical reactions: enzymes in biological washing powders break down protein, starch or fat stains on clothes, and enzymes in meat tenderizer break down proteins into smaller molecules, making the meat easier to chew.