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Mass-Action Ratios!
Mass-Action Ratios!

Amino Acids Analysis
Amino Acids Analysis

... The Amino Acids Analysis (Urine or Plasma) is an invaluable tool for evaluating dietary protein adequacy and assimilation, as well as metabolic imbalances underlying many chronic disorders. With the precise results and comprehensive commentary provided, nutritional deficits, metabolic impairments, a ...
Carbohydrate Metabolism
Carbohydrate Metabolism

... • The uronic acid pathway is a source of UDPglucuronate. • UDP-glucuronate is a precursor in biosynthesis of proteoglycans (glycosaminoglycans) and glycoproteins. • UDP-glucuronate is involved in detoxification reactions that occur in liver. Many naturally occurring waste substances (like bilirubin ...
intermediary metabolism
intermediary metabolism

... mutations of organisms, in which a given enzyme fails to be synthesized in active form. Such a defect, if not lethal, may result in the accumulation and excretion of the substrate of the defective enzyme. From genetic defects in specific enzymes occurring in people it has been possible for e.g., to ...
Student notes in ppt
Student notes in ppt

... PKU is an autosomal recessive genetic disease The phenylalanine hydroxylase gene is located on chromosome 12 making it an autosomal recessive genetic disease. An autosomal genetic disease is one in which the mutation is located on one of the 22 autosomal chromosomes (all chromosomes except the X or ...
GRN plugin The GRN query plugin expects to receive input data in
GRN plugin The GRN query plugin expects to receive input data in

... The application gives the user the options to select the databases of interest to retrieve the regulatory information from and also identify some parameters related to each one such as the species, and automatic update with every run. Additionally, the user can set the binding motif parameters such ...
Overview on Reactions with Multi
Overview on Reactions with Multi

... high affinity towards the enzyme, and it exhibits high activity even with such low substrate concentrations. Otherwise, too much enzyme would be consumed. Additionally, amino acid oxidases are competitively inhibited by a-keto acids,23,24 which would make a continuously operated enzyme membrane reac ...
Analysis of energy metabolism in acetic acid bacteria during
Analysis of energy metabolism in acetic acid bacteria during

... many useful materials such as l-sorbose and 6-amino-lsorbose, which are precursors of vitamin C and the anti-diabetic drug miglitol, respectively. Complete genome sequences of some acetic acid bacteria are now available and considerable biochemical research on these bacteria has been published. Howe ...
Chapter 2 The Chemistry of Life Worksheets
Chapter 2 The Chemistry of Life Worksheets

... that knowledge of chemistry as well as biology is needed to understand fully the life processes of organisms at the level of the cell. The sum of all the biochemical reactions in an organism is called metabolism. It includes both exothermic and endothermic reactions. Types of Biochemical Reactions E ...
Comparative genomics and metabolic reconstruction of
Comparative genomics and metabolic reconstruction of

... Comparative genomics and metabolic reconstruction of bacterial pathogens Mikhail Gelfand Institute for Information Transmission Problems, RAS ...
Lecture 11 (Parker) - Department of Chemistry ::: CALTECH
Lecture 11 (Parker) - Department of Chemistry ::: CALTECH

... Vitamins are organic molecules that are needed in small amounts in the diets of higher animals as higher animals have lost the capacity to synthesize these molecules during the course of evoluBon ...
File
File

... State that metabolic pathways consist of chains and cycles of enzyme-catalysed reactions. Describe the induced-fit model. (This is an extension of the lock-and-key model. Its importance in accounting for the ability of some enzymes to bind to several substrates should be mentioned). Explain that enz ...
AtMetExpress Development: A Phytochemical
AtMetExpress Development: A Phytochemical

B3. Enzymes - IGCSEBiology-Dnl
B3. Enzymes - IGCSEBiology-Dnl

... amount of energy required for chemical reaction to occur (activation energy)  this allows biochemical reactions to take place at a faster rate in the cells of living organisms at body temperature which is usually low  enzymes catalyse metabolic reactions (i.e. chemical reactions that sustain life) ...
QuASI: Question Answering using Statistics, Semantics, and
QuASI: Question Answering using Statistics, Semantics, and

... • Each sub-query returns a set of documents with a relevance score from the text search engine (or a fixed value for MeSH matches) • The aggregated score is the weighted SUM of the individual scores with optional weights applied to each sub-query score. • SUM performs better than MAX, since it gives ...
(i) Enzymes are (1)
(i) Enzymes are (1)

From phylogenetic trees to networks
From phylogenetic trees to networks

Fatty Acid & Protein Metabolism
Fatty Acid & Protein Metabolism

... • Ketoacidosis occurs in Type I Diabetes • Lack of insulin causes hyperglycemia • Dehydration and sweet taste to urine ...
Metabolic acidosis
Metabolic acidosis

... • Serum Osm = 2 (Na) + BUN/2.8 + glc/18 • Calculated and determined Osm should agree within 10 to 15 mOsm/kg. • If not, then serum Na may be spuriously low OR osmolytes other then Na, glc or urea have accumulated. • The osmolar gap is a reliable and helpful tool when screening for toxin-associated h ...


... Choice B: PFK-1 in glycolysis and fructose-bis-phosphatase-1 in gluconeogenesis are regulated both by energy sensing as well as by hormones. Briefly explain how these two pathways are regulated by one of these regulatory methods. You need not discuss how the levels of F2,6P are regulated, by you sho ...
Approach to inborn errors of metabolism presenting in the neonate
Approach to inborn errors of metabolism presenting in the neonate

... 5) The infant will need careful observation and follow-up for the first few months, as IEM may present in different age groups in members of the same family. Long term treatment of IEM The following modalities are available: 1) Dietary treatment: This is the mainstay of treatment in phenylketonuria, ...
Chapter6summaryHO
Chapter6summaryHO

... E.coli can do anaerobic and aerobic respiration, and fermentation. Streptococcus pneumoniae can only ferment because it lacks an electron transport chain. The only ATP generating pathway is glycolysis and involve substrate level phosphorylation. The other additional steps oxidize NADH to regenerate ...
Unit 3 (Bioenergetics) Objectives and Essay Samples
Unit 3 (Bioenergetics) Objectives and Essay Samples

... Explain the carbon fixing reactions (Calvin Cycle) in detail ...
Full-Text PDF
Full-Text PDF

... diagnostic tool within the right discovery context, thus, provides a powerful means to gain novel biological insight into the metabolic regulatory network. For this purpose, this review does not attempt to detail individual metabolite changes in response to abiotic stress conditions; for a recent co ...
Extra slides (lecture Mon. 11/2)
Extra slides (lecture Mon. 11/2)

... features in enzyme mechanisms • participation of multiple side chains (or cofactors makes concerted acid-base mechanisms possible (and common) for enzymes • the use of acid-base mechanisms is one reason the activities of most enzymes are pH dependence • details of the pH dependence can often be used ...
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Metabolic network modelling



Metabolic network reconstruction and simulation allows for an in-depth insight into the molecular mechanisms of a particular organism. In particular, these models correlate the genome with molecular physiology. A reconstruction breaks down metabolic pathways (such as glycolysis and the Citric acid cycle) into their respective reactions and enzymes, and analyzes them within the perspective of the entire network. In simplified terms, a reconstruction collects all of the relevant metabolic information of an organism and compiles it in a mathematical model. Validation and analysis of reconstructions can allow identification of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. This knowledge can then be applied to create novel biotechnology.In general, the process to build a reconstruction is as follows: Draft a reconstruction Refine the model Convert model into a mathematical/computational representation Evaluate and debug model through experimentation↑
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