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ducing antibodies with a slightly different configuration at the antigen-
ducing antibodies with a slightly different configuration at the antigen-

... Proteolytic fragments can be helpful in analyzing how antibodies bind in vitro. For example, to test whether the Fc piece is important for a particular function, you can compare intact antibody with F(ab )2 fragments of the same antibody. When using antibodies to stain cells in immunohistochemical o ...
Testosterone and sexual selection
Testosterone and sexual selection

... Sperm cells are non-self to die male and therefore targets of immunological attacks in the reproductive tract Consequently, sperm cells, and eventually ejaculate quality, are influenced by a male's ability to down-regulate immune responses during the production df ejaculates, a process potentially r ...
Bacterial Kidney Disease in salmonid fish
Bacterial Kidney Disease in salmonid fish

Immunology Project - Harvard Life Sciences Outreach Program
Immunology Project - Harvard Life Sciences Outreach Program

Negative-Strand RNA Viruses Maturation and Adaptive Immunity by
Negative-Strand RNA Viruses Maturation and Adaptive Immunity by

Transplantation: The replacement of diseased organs by a
Transplantation: The replacement of diseased organs by a

Gene-set - Bader Lab
Gene-set - Bader Lab

... How do pathways and processes differ? – In a purely biological perspective, the question is philosophical (still worth speculating…) – In a bioinformatics perspective: • A gene is annotated for a GO Biological Processes if the curators deem it (significantly) contributes to the process (which is at ...
Definition of a Conserved Immunodominant Domain on Hepatitis C
Definition of a Conserved Immunodominant Domain on Hepatitis C

... epitopes that are conserved among different HCV genotypes. Five human monoclonal antibodies (HMAbs) are described that cross-compete with other antibodies to a cluster of overlapping epitopes, previously designated domain B. Each HMAb broadly neutralizes retroviral pseudotype particles expressing HC ...
Susceptibility to tuberculosis is associated with variants in
Susceptibility to tuberculosis is associated with variants in

... the MHC region, none of those loci was significantly associated with TB after Bonferroni correction (Supplementary Tables 3 and 4). Our only genome-wide significant TB-associated locus was on chromosome 8q24, where 11 SNPs located in introns of the ASAP1 gene had P < 5 × 10−8 (Fig. 1); the strongest ...
Complexity in cancer stem cells and tumor evolution: towards
Complexity in cancer stem cells and tumor evolution: towards

... seems plausible that both factors contribute to metastasis. The possibility that cancer cells can switch into CSCs under specific conditions dictated by a depletion in the number of CSCs or by the environments, opens a new perspective on the metastatic process. The metastatic process is usually intr ...
Suppression of adaptive immunity to heterologous antigens during
Suppression of adaptive immunity to heterologous antigens during

... after LPS stimulation (Figure 2e,f), suggesting that the presence of parasites does not result in DC activation. The viability of treated DCs and control cells was quantified after 24 hours of culture by trypan blue exclusion (Figure 2g) and propidium iodide (PI) and annexin V staining (data not sho ...
Probing Cell Wall Structure and Development by
Probing Cell Wall Structure and Development by

... antibody to bind. Specific epitopes may be structurally altered or masked by other components in the system, and thus inaccessible to the antibody (Knox, 2008). For example, the pre-treatment of sections of plant material with pectate lyase [in N-cyclohexyl3-aminopropanesulfonic acid (CAPS) buffer] ...
CONNECTIVE TISSUE
CONNECTIVE TISSUE

... - fibrous tissue with fewer cells (cells are mostly fibroblasts) - collagen fibers are bundles, without definite orientation - found in dermis, prostate, mammary glands, outer capsule of many organs 2. dense regularly arranged connective tissue (DRACT) - made of many fibers that run in the same dire ...
Aplastic anemia (AA) is a bone marrow failure disease, which mainly
Aplastic anemia (AA) is a bone marrow failure disease, which mainly

... be that the generation of CD4+ cells depend mainly on the thymus, while the formation and maturation of CD8+ cells are not completely dependent on the thymus[10]. Related research[11] showed that, lymphocyte subsets especially the dynamic balance of T lymphocyte subsets was related to immune functio ...
Successful Plating Strategies
Successful Plating Strategies

... yielded a strong response, the fusion should be repeated, but the choice of screening method should be re-evaluated. If the immune response was weak, new approaches to the immunization should be tried. If only a few positive clones were identified, these should be tested as early as possible to dete ...
Characterization of immune cells in psoriatic adipose tissue
Characterization of immune cells in psoriatic adipose tissue

... Immunophenotyping studies of human adipose have generally assumed that characteristic markers of immune cells described in animal models or in other human tissues can be applied similarly to adipose tissue. A major limitation of prior studies has been a paucity of data confirming flow cytometric ana ...
The Antinociceptive Effect of Dexmedetomidine Modulates Spleen
The Antinociceptive Effect of Dexmedetomidine Modulates Spleen

... injury through nociceptor-induced sympathetic activation, because immune cells of lymphoid organs express adrenorecptors [1]. Generally, pain enhances the cytotoxic activity of natural killer (NK) cells, the lymphoproliferative response of lymphoid tissue, and cytokine production by immune cells [2, ...
Postprint
Postprint

... was detectable both for the number of lesional parasites in infected skin (left panel) or the degree of parasite dissemination into spleen, which is a prominent feature of visceral leishmaniasis (right panel). Even though IL-22 does not directly signal to immune cells, it can initiate skin inflammat ...
Human Leukocyte Antigen-Class II-Positive
Human Leukocyte Antigen-Class II-Positive

... In the cornea, it is thought that Langerhans cells, which exist in limbal and peripheral cornea, can be potent APCs. It has been shown that rabbit corneal endothelium, expressing class II antigen after treatment with IFN-y, could stimulate fresh allogenic lymphocytes.25 On the other hand, human corn ...
Laughter..the best medicine!
Laughter..the best medicine!

innate immunity in c. elegans
innate immunity in c. elegans

... are absent from the C. elegans genome. One prominent class of PRRs, in vertebrates the TLRs, can sense outer membrane components of the bacteria, RNA or DNA.22 As mentioned above, the single worm TLR, TOL‑1, is involved in behavioural avoidance of some pathogenic bacteria,4,5 but does not seem to pl ...
Surface Protein-1 Carboxyl-Terminal Fragment of Merozoite
Surface Protein-1 Carboxyl-Terminal Fragment of Merozoite

... passively protected by adoptively transferred Abs. B10.BR mice are not protected following vaccination with GST-MSP119 (6). The reasons for this are not clear, but may relate to the titer of Ab induced by vaccination or to other factors such as the fine specificity of the Ab response. However, these ...
Article 1: Review - Cigarette Smoking and Innate Immunity
Article 1: Review - Cigarette Smoking and Innate Immunity

Airway smooth muscle cells respond directly to inhaled
Airway smooth muscle cells respond directly to inhaled

... Asthma was first described as a pathology of the airway smooth muscle since all asthma cases examined presented one unique pathology, an increased mass of airway smooth muscle bundles [23]. Today an increasing number of publications have provided evidence on both the clinical and experimental level ...
Chapter 5 Protein Function
Chapter 5 Protein Function

... surface of the killer T cells called T-cell receptors • The TH cells produce soluble signaling proteins called cytokines that interact with other proteins and cells of the immune system Chapter 5 ...
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Immunomics

Immunomics is the study of immune system regulation and response to pathogens using genome-wide approaches. With the rise of genomic and proteomic technologies, scientists have been able to visualize biological networks and infer interrelationships between genes and/or proteins; recently, these technologies have been used to help better understand how the immune system functions and how it is regulated. Two thirds of the genome is active in one or more immune cell types and less than 1% of genes are uniquely expressed in a given type of cell. Therefore, it is critical that the expression patterns of these immune cell types be deciphered in the context of a network, and not as an individual, so that their roles be correctly characterized and related to one another. Defects of the immune system such as autoimmune diseases, immunodeficiency, and malignancies can benefit from genomic insights on pathological processes. For example, analyzing the systematic variation of gene expression can relate these patterns with specific diseases and gene networks important for immune functions.Traditionally, scientists studying the immune system have had to search for antigens on an individual basis and identify the protein sequence of these antigens (“epitopes”) that would stimulate an immune response. This procedure required that antigens be isolated from whole cells, digested into smaller fragments, and tested against T- and B-cells to observe T- and B- cell responses. These classical approaches could only visualize this system as a static condition and required a large amount of time and labor.Immunomics has made this approach easier by its ability to look at the immune system as a whole and characterize it as a dynamic model. It has revealed that some of the immune system’s most distinguishing features are the continuous motility, turnover, and plasticity of its constituent cells. In addition, current genomic technologies, like microarrays, can capture immune system gene expression over time and can trace interactions of microorganisms with cells of the innate immune system. New, proteomic approaches, including T-cell and B-cells-epitope mapping, can also accelerate the pace at which scientists discover antibody-antigen relationships.
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