Abstract About the Speaker Rocks, clots, and fertility: Fetuin family
... exon shuffling within the cystatin superfamily of genes. The genes form a cluster of type III cystatin proteins (hepatic, constitutively secreted) in the mouse and human genome. We study the structure-function relationship of fetuin-A, fetuin-B and HRG by gene knockout in mice. Fetuin-A is a serum p ...
... exon shuffling within the cystatin superfamily of genes. The genes form a cluster of type III cystatin proteins (hepatic, constitutively secreted) in the mouse and human genome. We study the structure-function relationship of fetuin-A, fetuin-B and HRG by gene knockout in mice. Fetuin-A is a serum p ...
chapter3_Sections 4
... linear sequence of amino acids (a polypeptide chain). Each type of protein has a unique primary structure. ...
... linear sequence of amino acids (a polypeptide chain). Each type of protein has a unique primary structure. ...
Fluorescent proteins Green Fluorescence Protein
... biological science by providing a way to monitor how individual genes are regulated and expressed within a living cell ; Localization and tracing of a target protein • Widespread use by their expression in other organisms as a reporter • Usually fused to N- or C-terminus of proteins by gene manipula ...
... biological science by providing a way to monitor how individual genes are regulated and expressed within a living cell ; Localization and tracing of a target protein • Widespread use by their expression in other organisms as a reporter • Usually fused to N- or C-terminus of proteins by gene manipula ...
SG-Glutamic-C™ (Cat. # 786-15)
... SG-Glutamic-C is a sequencing grade serine endopeptidase, from S. aureus V8 that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In ammonium bicarbonate or Tris-HCl buffer, in particular in the absence of pho ...
... SG-Glutamic-C is a sequencing grade serine endopeptidase, from S. aureus V8 that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In ammonium bicarbonate or Tris-HCl buffer, in particular in the absence of pho ...
Van der Waals bonds
... • Not all proteins can be crystallized, though, and even if they are, it is possible that the conformation in the crystal form is different from that in solution. In recent years nuclear magnetic resonance (NMR) spectroscopy has been successful in identifying the tertiary structure of some proteins ...
... • Not all proteins can be crystallized, though, and even if they are, it is possible that the conformation in the crystal form is different from that in solution. In recent years nuclear magnetic resonance (NMR) spectroscopy has been successful in identifying the tertiary structure of some proteins ...
Gelbart_040528
... Pool resources - compute common set of similarities a) + Consistency b) - Some MOD group has to run the computes c) - Stalinistic ...
... Pool resources - compute common set of similarities a) + Consistency b) - Some MOD group has to run the computes c) - Stalinistic ...
Protein folding and movement in the bacterial cell The action of
... because they lack SS $They also fold quickly… • SS target polypeptides to export (or secretion/Sec) machinery • Export machinery (Sec apparatus) depends on unfolded state of polypeptide for localization $SS can antagonize folding of protein… ...
... because they lack SS $They also fold quickly… • SS target polypeptides to export (or secretion/Sec) machinery • Export machinery (Sec apparatus) depends on unfolded state of polypeptide for localization $SS can antagonize folding of protein… ...
Poster - Protein Information Resource
... framework. Expert manual curation includes membership, annotation of specific biological functions, biochemical activities, and sequence features. Novel functional predictions for uncharacterized “hypothetical” proteins and protein families are routinely made in the annotation process. Fully curated ...
... framework. Expert manual curation includes membership, annotation of specific biological functions, biochemical activities, and sequence features. Novel functional predictions for uncharacterized “hypothetical” proteins and protein families are routinely made in the annotation process. Fully curated ...
Functional domains of the BRCA2 protein
... Published in Expert Reviews in Molecular Medicine by Cambridge University Press (2001) ...
... Published in Expert Reviews in Molecular Medicine by Cambridge University Press (2001) ...
Gene Section AKAP12 (A kinase (PRKA) anchor protein 12)
... A: Except for testes, most cells express four major isoforms of AKAP12 protein. The 305 kDa isoforms is the myristylated AKAP12alpha whereas the 287 kDa isoforms is AKAP12beta. The 250 kDa and 43 kDa isoforms are proteolytic cleavage products common to the AKAP12alpha and beta isoforms. B: Human AKA ...
... A: Except for testes, most cells express four major isoforms of AKAP12 protein. The 305 kDa isoforms is the myristylated AKAP12alpha whereas the 287 kDa isoforms is AKAP12beta. The 250 kDa and 43 kDa isoforms are proteolytic cleavage products common to the AKAP12alpha and beta isoforms. B: Human AKA ...
Spectrophotometric methods for determination of proteins
... characteristic rather than on some quantity or measured value. It can be a form of analysis that yields the identity of a compound. ...
... characteristic rather than on some quantity or measured value. It can be a form of analysis that yields the identity of a compound. ...
Heterologous expression and purification of proteins in E. coli
... N-terminus or C-terminal tags – what is known about protein function? – modified N-terminus can have big consequences: Met-RANTES – use a cleavable tag with a good protease like enterokinase or TEV ...
... N-terminus or C-terminal tags – what is known about protein function? – modified N-terminus can have big consequences: Met-RANTES – use a cleavable tag with a good protease like enterokinase or TEV ...
Getting the most out of milk
... tertiary structure. These interact physically and chemically with other such structures in a specific manner, called the quaternary structure. This forms the active protein. ...
... tertiary structure. These interact physically and chemically with other such structures in a specific manner, called the quaternary structure. This forms the active protein. ...
Searching for the key to bone formation
... constitute bones in mammals. Therefore, we propose that the function of this protein is very important in determining if cells will be competent in constructing osseous tissue, in addition to later helping to maintain the skeleton strong and healthy throughout an individual’s lifetime,” explains Dr. ...
... constitute bones in mammals. Therefore, we propose that the function of this protein is very important in determining if cells will be competent in constructing osseous tissue, in addition to later helping to maintain the skeleton strong and healthy throughout an individual’s lifetime,” explains Dr. ...
BNFO 602 Lecture 1 - New Jersey Institute of Technology
... T (thymine) and C (cytosine) always pairs with G (guanine) knowing only one side of the ladder is enough • We represent DNA as a sequence of letters where each letter could be A,C,G, or T. • For example, for the helix shown here we would represent this as CAGT. ...
... T (thymine) and C (cytosine) always pairs with G (guanine) knowing only one side of the ladder is enough • We represent DNA as a sequence of letters where each letter could be A,C,G, or T. • For example, for the helix shown here we would represent this as CAGT. ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... PART - B Answer the following in about 500 words only; Draw diagrams wherever necessary: ( 5 x 8 = 40 marks) 21 a) Enlist the modern version of cell theory. OR b) Write an account of extracellular matrix. 22 a) Explain the galactose metabolism in yeast. OR b) Give an account of various RNA processin ...
... PART - B Answer the following in about 500 words only; Draw diagrams wherever necessary: ( 5 x 8 = 40 marks) 21 a) Enlist the modern version of cell theory. OR b) Write an account of extracellular matrix. 22 a) Explain the galactose metabolism in yeast. OR b) Give an account of various RNA processin ...
Gene Regulation - Marblehead High School
... His parents do not have this condition Your Assignment: Do your medical research to find out the cause of this student’s situation. Describe what is happening to him and explain ...
... His parents do not have this condition Your Assignment: Do your medical research to find out the cause of this student’s situation. Describe what is happening to him and explain ...
HANDOUT: CH 18 pt 1 Study
... Regulation of Gene Expression: Prokaryotes vs. Eukaryotes (p. 351-366) 1) What are the two levels within which metabolic control can occur in bacteria? ...
... Regulation of Gene Expression: Prokaryotes vs. Eukaryotes (p. 351-366) 1) What are the two levels within which metabolic control can occur in bacteria? ...
CHAPTER 16
... (but a different one for each of the many different proteins, which have different amino acid sequences)? The way to surmount this analytical problem, of course, was to look at the synthesis of a single protein, without “interference” from other proteins. It is in understanding the importance of th ...
... (but a different one for each of the many different proteins, which have different amino acid sequences)? The way to surmount this analytical problem, of course, was to look at the synthesis of a single protein, without “interference” from other proteins. It is in understanding the importance of th ...
Transgenic organisms - Ken Pitts` Biological Science Page
... IL-10 (one from a virus, one from the mouse) and generated plants in which this protein was targeted to three different compartments within the cell, to see which would work most effectively.The researchers found that tobacco plants were able to process both forms of IL-10 correctly, producing the a ...
... IL-10 (one from a virus, one from the mouse) and generated plants in which this protein was targeted to three different compartments within the cell, to see which would work most effectively.The researchers found that tobacco plants were able to process both forms of IL-10 correctly, producing the a ...
Macronutrients: Carbs, Proteins and Fats
... are considered “good” or “bad”, how much of each we are supposed to consume and what are the ideal ratios. First of all, it is important to distinguish fad dieting from developing good, healthy longterm eating habits. Sure, fad diets may help you lose weight in the short-term, but this singleminded ...
... are considered “good” or “bad”, how much of each we are supposed to consume and what are the ideal ratios. First of all, it is important to distinguish fad dieting from developing good, healthy longterm eating habits. Sure, fad diets may help you lose weight in the short-term, but this singleminded ...
the-cell-factory Excellent
... carbohydrates, and proteins into smaller molecules that can be used by the cell Also digest organelles that have outlived their usefulness ...
... carbohydrates, and proteins into smaller molecules that can be used by the cell Also digest organelles that have outlived their usefulness ...
Caenorhabditis Annotation in UniProtKB
... The nematode worm, C.elegans, is a transparent soil dwelling roundworm. It is approximately 1mm in length and has a relatively short life cycle and thus it is a versatile model organism for studying gene and protein function in complex biological processes. The use of this model is adopted by thousa ...
... The nematode worm, C.elegans, is a transparent soil dwelling roundworm. It is approximately 1mm in length and has a relatively short life cycle and thus it is a versatile model organism for studying gene and protein function in complex biological processes. The use of this model is adopted by thousa ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.