viral networks
... http://www.dnatube.com/video/993/Plasmid• Only need the sequenced genome (or sequence of Cloning interest) • Scalable, its possible to screen for interactions among many proteins creating a more high-throughput screen (ex. viral genome) • Protein/polypeptides can be from various sources; eukaryotes, ...
... http://www.dnatube.com/video/993/Plasmid• Only need the sequenced genome (or sequence of Cloning interest) • Scalable, its possible to screen for interactions among many proteins creating a more high-throughput screen (ex. viral genome) • Protein/polypeptides can be from various sources; eukaryotes, ...
The DNA Structure
... created single gene mutations that incapacitated specific enzymes, so that the molds with these mutations required an external supply of the substance that the enzyme normally produced, and the substance that the enzyme normally used, piled up in the cell • These results confirmed their one geneone ...
... created single gene mutations that incapacitated specific enzymes, so that the molds with these mutations required an external supply of the substance that the enzyme normally produced, and the substance that the enzyme normally used, piled up in the cell • These results confirmed their one geneone ...
argC Orthologs from Rhizobiales Show Diverse Profiles of
... loti). The argC gene is necessary for the synthesis of arginine, an amino acid that is central to protein and cellular metabolism. Strains were obtained carrying plasmids with argC orthologs expressed under the speB and argC (S. meliloti) and lac (Escherichia coli) promoters. Complementation analysi ...
... loti). The argC gene is necessary for the synthesis of arginine, an amino acid that is central to protein and cellular metabolism. Strains were obtained carrying plasmids with argC orthologs expressed under the speB and argC (S. meliloti) and lac (Escherichia coli) promoters. Complementation analysi ...
domain_searching.pdf
... Humans have 1.8 times as many protein architectures as fly or worm and 5.8 times as many as yeast. The increase in protein architectures is particularly evident in the development of new extracellular and transmembrane architectures. ...
... Humans have 1.8 times as many protein architectures as fly or worm and 5.8 times as many as yeast. The increase in protein architectures is particularly evident in the development of new extracellular and transmembrane architectures. ...
Bovine prolactin soluble receptor ECD ECD-11
... and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, s ...
... and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, s ...
chapter 6
... 9. List the components of the endomembrane system, and describe the structure and functions of each component. 10. Compare the structure and functions of smooth and rough ER. 11. Explain the significance of the cis and trans sides of the Golgi apparatus. 12. Describe three examples of intracellular ...
... 9. List the components of the endomembrane system, and describe the structure and functions of each component. 10. Compare the structure and functions of smooth and rough ER. 11. Explain the significance of the cis and trans sides of the Golgi apparatus. 12. Describe three examples of intracellular ...
Bioinformatics: Integrative Analyses of Genome
... (developing a database framework for classifying motions in terms of packing), and expression analysis (relating gene expression to protein subcellular localization). The ongoing research program in the lab extends and expands previous work as described below. Our work is fundamentally data-driven a ...
... (developing a database framework for classifying motions in terms of packing), and expression analysis (relating gene expression to protein subcellular localization). The ongoing research program in the lab extends and expands previous work as described below. Our work is fundamentally data-driven a ...
Toward a New Theoretical Framework for Biology
... Specificity of interaction between proteins or between enzymes and their substrates depends on precise molecular fit, complementary shapes that are stabilized by a number of weak (~0.1 -5 kcal/ mole) inter-atomic bonds. How is a protein’s shape, and hence its function, encoded? The ultimate, functio ...
... Specificity of interaction between proteins or between enzymes and their substrates depends on precise molecular fit, complementary shapes that are stabilized by a number of weak (~0.1 -5 kcal/ mole) inter-atomic bonds. How is a protein’s shape, and hence its function, encoded? The ultimate, functio ...
and y-crystallin X - Prof. N. Srinivasan
... many aspartates have been replaced with glutamates. These differences in charge distribution may be related to the different locations of these proteins in the lens. y-Crystallins are found mainly in the central densely packed core region of the lens, whereas yS-crystallin occurs in the more hydrate ...
... many aspartates have been replaced with glutamates. These differences in charge distribution may be related to the different locations of these proteins in the lens. y-Crystallins are found mainly in the central densely packed core region of the lens, whereas yS-crystallin occurs in the more hydrate ...
Chapter 12 Lecture Notes: The Nature of the Gene I. How genes
... Benzer wanted to know if recombination could occur within genes. He used his T4 rIIA mutant phage for recombination analysis, looking for rIIA+ recombinants that would form wildtype plaques on E. coli K. He was able to detect these very rare recombinants due to the incredably high sensitivity of his ...
... Benzer wanted to know if recombination could occur within genes. He used his T4 rIIA mutant phage for recombination analysis, looking for rIIA+ recombinants that would form wildtype plaques on E. coli K. He was able to detect these very rare recombinants due to the incredably high sensitivity of his ...
tpj12930-sup-0001-FigS1
... and subjected to gPCR using specific primers for both CA2 and CAL2 or CAL1 genes and T-DNA border primers (LB). Double homozygous plants were identified for ca2cal2 and ca2cal1 double mutants. (b) Transcript levels of WT and double mutant ca2cal1 plants were assessed by RT-PCR at 40 cycles using spe ...
... and subjected to gPCR using specific primers for both CA2 and CAL2 or CAL1 genes and T-DNA border primers (LB). Double homozygous plants were identified for ca2cal2 and ca2cal1 double mutants. (b) Transcript levels of WT and double mutant ca2cal1 plants were assessed by RT-PCR at 40 cycles using spe ...
PCB 6528 Exam – Organelle genomes and gene expression
... 3 [50 pt] In the table below, list the molecular processes that contribute to organelle gene expression resulting in fully assembled respiratory and photosynthetic complexes. Indicate which processes are currently known to involve proteins of the pentatricopeptide repeat (PPR) class. For those proce ...
... 3 [50 pt] In the table below, list the molecular processes that contribute to organelle gene expression resulting in fully assembled respiratory and photosynthetic complexes. Indicate which processes are currently known to involve proteins of the pentatricopeptide repeat (PPR) class. For those proce ...
Macromolecules of the Cell
... formation of two structural patterns, referred to as a-helix and B-sheet conformations. Alpha-helix pattern: Every peptide bond in the helix is H-bonded through its CO group to the peptide bond immediately below it in the spiral and through its NH group to the peptide bond just above it. B-sheet pat ...
... formation of two structural patterns, referred to as a-helix and B-sheet conformations. Alpha-helix pattern: Every peptide bond in the helix is H-bonded through its CO group to the peptide bond immediately below it in the spiral and through its NH group to the peptide bond just above it. B-sheet pat ...
Lecture 3
... macromolecules to form complex polysaccharides: Glycoproteins: polysaccharides + proteins Glycolipids: polysaccharides + lipids Cellular functions Cell-surface receptor molecules; typically reside on external surfaces of the membrane Glycolipids important in cell walls of gram-negative bac ...
... macromolecules to form complex polysaccharides: Glycoproteins: polysaccharides + proteins Glycolipids: polysaccharides + lipids Cellular functions Cell-surface receptor molecules; typically reside on external surfaces of the membrane Glycolipids important in cell walls of gram-negative bac ...
Slides
... involved in certain pathways and disease states. However, differences in the half-lives of RNA and proteins, as well as post-translational modifications important to protein function prevent mRNA profiles from being perfectly correlated to the cells’ actual protein profiles. ...
... involved in certain pathways and disease states. However, differences in the half-lives of RNA and proteins, as well as post-translational modifications important to protein function prevent mRNA profiles from being perfectly correlated to the cells’ actual protein profiles. ...
The samples were dissolved in 25 µL of 100 mM ammonium
... Each sample was split equally (10 µL/tube) into 2 tubes. One sample was digested with trypsin and the other tube was digested with LysC. The digestion protocol was the same for both enzymes and involved first diluting the sample 1:1 with 2,2,2-trifluoroethanol, then reducing with DTT at 90°C for 20 ...
... Each sample was split equally (10 µL/tube) into 2 tubes. One sample was digested with trypsin and the other tube was digested with LysC. The digestion protocol was the same for both enzymes and involved first diluting the sample 1:1 with 2,2,2-trifluoroethanol, then reducing with DTT at 90°C for 20 ...
DNA AND PROTEIN SYNTHESIS
... (methionine) to the ribosome. • Each tRNA carries one type of amino acid. • The anticodon (three nitrogen bases on tRNA) must complement codon for amino acid to be added to protein chain ...
... (methionine) to the ribosome. • Each tRNA carries one type of amino acid. • The anticodon (three nitrogen bases on tRNA) must complement codon for amino acid to be added to protein chain ...
Biology II – Chapter 9: DNA: The Molecule of Heredity
... o A single faulty gene may be more serious because all of the proteins synthesized according to the base sequence will be defective o Mutations that occur in gamete cells can be passed on to future generations if not lethal. o 4 Types of Mutation: Point Mutation – or nucleotide substitution – when ...
... o A single faulty gene may be more serious because all of the proteins synthesized according to the base sequence will be defective o Mutations that occur in gamete cells can be passed on to future generations if not lethal. o 4 Types of Mutation: Point Mutation – or nucleotide substitution – when ...
Genetic Determinants of Neurological Disorders -
... patterns silence gene transcription, leading to reduced levels of FMR-1 protein. II. Most Complex Heritable Phenotypes in Humans are Multigenic. Multigenic includes both oligogenic and polygenic traits. An oligogenic trait or disorder is determined by a small number of genes, each contributing to th ...
... patterns silence gene transcription, leading to reduced levels of FMR-1 protein. II. Most Complex Heritable Phenotypes in Humans are Multigenic. Multigenic includes both oligogenic and polygenic traits. An oligogenic trait or disorder is determined by a small number of genes, each contributing to th ...
Enzyme Units FAQ
... An enzyme is usually valued for its function and activity rather than its mass, therefore enzyme units serve as a quantification of enzyme activity. For many enzymes, the activity under specified conditions can be expressed in International Units (IU), which is defined as the amount of enzyme that c ...
... An enzyme is usually valued for its function and activity rather than its mass, therefore enzyme units serve as a quantification of enzyme activity. For many enzymes, the activity under specified conditions can be expressed in International Units (IU), which is defined as the amount of enzyme that c ...
Chapter 3 Presentation: Macromolecules
... • The secondary structure of the polypeptide is the coiled and/or folded patterns that emerges as the amino acids begin to interact with one another (H-bonds) not from the R-groups, but from the backbones of the amino acids. ...
... • The secondary structure of the polypeptide is the coiled and/or folded patterns that emerges as the amino acids begin to interact with one another (H-bonds) not from the R-groups, but from the backbones of the amino acids. ...
Télécharger la version pdf
... photosynthesis. To this end, plants and algae use internal cell structures called chloroplasts, which are equipped with protein complexes, the photosystems. If certain nutrients are missing, such as iron, the alga temporarily dismantles its photosystems to recycle some of their components. Researche ...
... photosynthesis. To this end, plants and algae use internal cell structures called chloroplasts, which are equipped with protein complexes, the photosystems. If certain nutrients are missing, such as iron, the alga temporarily dismantles its photosystems to recycle some of their components. Researche ...
Heterologous Protein Production in Eukaryotic Cells
... Strain containing particular marker (LEU) is auxotropic for Leu. This strain carries a mutation inactivating isopropylmalate dehydrogenase that renders it unable to synthesize leucine and will only be able to grow if Leu can be taken up from the medium during cultivation. ...
... Strain containing particular marker (LEU) is auxotropic for Leu. This strain carries a mutation inactivating isopropylmalate dehydrogenase that renders it unable to synthesize leucine and will only be able to grow if Leu can be taken up from the medium during cultivation. ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.